Plant Circular RNA Database

Detail information of Zm00001d045516_circ_g.3

			
						 General Information
					
						CircRNA Name
						Zm00001d045516_circ_g.3
					
						ID in PlantcircBase
						zma_circ_002917
					
						Alias
						circ1373
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 25243028-25244407  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Zm00001d045516						
					
						Parent gene annotation
						
							Probable transcriptional regulator SLK2
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d045516_circ_g.1 Zm00001d045516_circ_g.2 						
					
						Support reads
						9
					
						Tissues
						seedling leaves
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-CA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d045516_T012:3
Zm00001d045516_T018:4
Zm00001d045516_T023:2
Zm00001d045516_T014:3
Zm00001d045516_T022:4
Zm00001d045516_T010:3
Zm00001d045516_T019:3
Zm00001d045516_T002:3
Zm00001d045516_T015:3
Zm00001d045516_T020:3
Zm00001d045516_T005:4
Zm00001d045516_T003:3
Zm00001d045516_T013:3
Zm00001d045516_T001:3
Zm00001d045516_T021:2
Zm00001d045516_T004:3
Zm00001d045516_T008:3
Zm00001d045516_T006:3
Zm00001d045516_T011:3

General Information
CircRNA Name	Zm00001d045516_circ_g.3
ID in PlantcircBase	zma_circ_002917
Alias	circ1373
Organism	Zea mays
Position	chr9: 25243028-25244407 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI
Parent gene	Zm00001d045516
Parent gene annotation	Probable transcriptional regulator SLK2
Parent gene strand	+
Alternative splicing	Zm00001d045516_circ_g.1 Zm00001d045516_circ_g.2
Support reads	9
Tissues	seedling leaves
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-CA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d045516_T012:3 Zm00001d045516_T018:4 Zm00001d045516_T023:2 Zm00001d045516_T014:3 Zm00001d045516_T022:4 Zm00001d045516_T010:3 Zm00001d045516_T019:3 Zm00001d045516_T002:3 Zm00001d045516_T015:3 Zm00001d045516_T020:3 Zm00001d045516_T005:4 Zm00001d045516_T003:3 Zm00001d045516_T013:3 Zm00001d045516_T001:3 Zm00001d045516_T021:2 Zm00001d045516_T004:3 Zm00001d045516_T008:3 Zm00001d045516_T006:3 Zm00001d045516_T011:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GATATATAAAAACACGATTTCATGCAATTTCTTATATTCTCTATGGTACAGGATACGTGGCGAT
GTGGCATTTGCAATACACATGGTGGGAAAGGATATGggttctggctaaagtacgttaaggtctt
ttttatcagcaattccagtaatgtgccttgcctcttttacgtgactatacaagacaatgaatgg
aactcatc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GGTTCTGGCTAAAGTACGTTAAGGTCTTTTTTATCAGCAATTCCAGTAATGTGCCTTGCCTCTT
TTACGTGACTATACAAGACAATGAATGGAACTCATCCCTGGGCCTCCATGTCCGGAGGTCCATG
TTCTAACCTTGGATATGCCAGGGACATGAATGGTAATGTTCCAATAAGCACTACAAATTCTTCT
GGGCCAAGCATTGGAGTTAGCTCATTGGTGACTGACGCCAACTCGTCACTTTCCGGTGGTGCCC
AGTTACAGCCAAGTACTAGCATGAATGGTGATTCATTGATGCGTGTTCCTGCGTCACCAATATC
GTTTTCATCCAATAACATCTCTGGCTCTTCAGTCATTGACGGTTGCATCATGCAGCAAAGTCCA
CCCCAAGAGCAGGTGCAGAAACAGAGGTCTTCAAGTGTAACATCACAACCTGTGATTGATGCTG
GTGGTGCATTGCATGCTCAGAAGAAATCAAGGATTGATGTTTGTCAGGGTGGTATCACGGAACA
ACAGCTGATTCAGCAACTGCTCCATGGTCAGAATTCTCTTCATTACCAGGGGCAACAGAATCCA
CAGCTTCAAGCTTTAATGCAGCATCATAAACTGGCACAACTTCAGCAACGGCAGCAGCAGCATC
CATTTTCTCAGATGCAACAACCCCAAGTTGGTATTCCTCGGCAGCCTCAACTTAGGCCACCACT
GGCACAGACTGGAATGCAGTTAGGTGGTCCTGTTAGGTCTCCTATCGAGAGTGGGATTTGTTCT
CGCAGGATACTGCAGTATTTATATCACAAGCGTCATCGCCCAGAGGTGAGTTTCTCTATAGTAT
TAATTGTTTGTGGGATTATGAGGTACAAGGTATTGACAGTACAATTAATTTGAATGGTGTTTTC
TAGAATAACCCTATAACATACTGGAGGAAGCTTGTTGAAGAGTACTTCGCACCTCGAGCAAGAG
AAAGATGGTGTGTCTCTTCTTATGAAAACAGAGGGAATTCTTCAGCTGCTGCTCCACAGAAAGC
TTTGGTAATTAATACTTCACACACTTGTATTTTACTTCCCATTGTATAGTGCTCTTTATTTCAT
GGCTGGACATCAGTTTCTGCTTGATGTTCAGTATTAGATTGATTAGCATCACTGTGGAGTTCTT
CTTGGGTTACTGAAAACCACATTTAATTTATTATGTGCCCTTAATGGTAGGAAAATAGATCTTG
TATGCACAACTTTTTGCAGTAAAGAGTTCCATTGTAGTCTGCAGCAGTTAAAATTTCCTTTTTC
GATATATAAAAACACGATTTCATGCAATTTCTTATATTCTCTATGGTACAGGATACGTGGCGAT
GTGGCATTTGCAATACACATGGTGGGAAAGGATATG

Sequences
Splice junction sequence	GATATATAAAAACACGATTTCATGCAATTTCTTATATTCTCTATGGTACAGGATACGTGGCGAT GTGGCATTTGCAATACACATGGTGGGAAAGGATATGggttctggctaaagtacgttaaggtctt ttttatcagcaattccagtaatgtgccttgcctcttttacgtgactatacaagacaatgaatgg aactcatc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGTTCTGGCTAAAGTACGTTAAGGTCTTTTTTATCAGCAATTCCAGTAATGTGCCTTGCCTCTT TTACGTGACTATACAAGACAATGAATGGAACTCATCCCTGGGCCTCCATGTCCGGAGGTCCATG TTCTAACCTTGGATATGCCAGGGACATGAATGGTAATGTTCCAATAAGCACTACAAATTCTTCT GGGCCAAGCATTGGAGTTAGCTCATTGGTGACTGACGCCAACTCGTCACTTTCCGGTGGTGCCC AGTTACAGCCAAGTACTAGCATGAATGGTGATTCATTGATGCGTGTTCCTGCGTCACCAATATC GTTTTCATCCAATAACATCTCTGGCTCTTCAGTCATTGACGGTTGCATCATGCAGCAAAGTCCA CCCCAAGAGCAGGTGCAGAAACAGAGGTCTTCAAGTGTAACATCACAACCTGTGATTGATGCTG GTGGTGCATTGCATGCTCAGAAGAAATCAAGGATTGATGTTTGTCAGGGTGGTATCACGGAACA ACAGCTGATTCAGCAACTGCTCCATGGTCAGAATTCTCTTCATTACCAGGGGCAACAGAATCCA CAGCTTCAAGCTTTAATGCAGCATCATAAACTGGCACAACTTCAGCAACGGCAGCAGCAGCATC CATTTTCTCAGATGCAACAACCCCAAGTTGGTATTCCTCGGCAGCCTCAACTTAGGCCACCACT GGCACAGACTGGAATGCAGTTAGGTGGTCCTGTTAGGTCTCCTATCGAGAGTGGGATTTGTTCT CGCAGGATACTGCAGTATTTATATCACAAGCGTCATCGCCCAGAGGTGAGTTTCTCTATAGTAT TAATTGTTTGTGGGATTATGAGGTACAAGGTATTGACAGTACAATTAATTTGAATGGTGTTTTC TAGAATAACCCTATAACATACTGGAGGAAGCTTGTTGAAGAGTACTTCGCACCTCGAGCAAGAG AAAGATGGTGTGTCTCTTCTTATGAAAACAGAGGGAATTCTTCAGCTGCTGCTCCACAGAAAGC TTTGGTAATTAATACTTCACACACTTGTATTTTACTTCCCATTGTATAGTGCTCTTTATTTCAT GGCTGGACATCAGTTTCTGCTTGATGTTCAGTATTAGATTGATTAGCATCACTGTGGAGTTCTT CTTGGGTTACTGAAAACCACATTTAATTTATTATGTGCCCTTAATGGTAGGAAAATAGATCTTG TATGCACAACTTTTTGCAGTAAAGAGTTCCATTGTAGTCTGCAGCAGTTAAAATTTCCTTTTTC GATATATAAAAACACGATTTCATGCAATTTCTTATATTCTCTATGGTACAGGATACGTGGCGAT GTGGCATTTGCAATACACATGGTGGGAAAGGATATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.115341896

Conservation Information
Conserved circRNAs	NA
PMCS	0.115341896

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							25243186-25243158(+)
25244010-25243036(+)
						
						Potential amino acid sequence
						
							MPSTSMNGDSLMRVPASPISFSSNNISGSSVIDGCIMQQSPPQEQVQKQRSSSVTSQPVIDAGG
ALHAQKKSRIDVCQGGITEQQLIQQLLHGQNSLHYQGQQNPQLQALMQHHKLAQLQQRQQQHPF
SQMQQPQVGIPRQPQLRPPLAQTGMQLGGPVRSPIESGICSRRILQYLYHKRHRPENNPITYWR
KLVEEYFAPRARERWCVSSYENRGNSSAAAPQKALDTWRCGICNTHGGKGYGFWLKYVKVFFIS
NSSNVPCLFYVTIQDNEWNSSLGLHVRRSMF*(+)
MKTEGILQLLLHRKLWIRGDVAFAIHMVGKDMGSG*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	25243186-25243158(+) 25244010-25243036(+)
Potential amino acid sequence	MPSTSMNGDSLMRVPASPISFSSNNISGSSVIDGCIMQQSPPQEQVQKQRSSSVTSQPVIDAGG ALHAQKKSRIDVCQGGITEQQLIQQLLHGQNSLHYQGQQNPQLQALMQHHKLAQLQQRQQQHPF SQMQQPQVGIPRQPQLRPPLAQTGMQLGGPVRSPIESGICSRRILQYLYHKRHRPENNPITYWR KLVEEYFAPRARERWCVSSYENRGNSSAAAPQKALDTWRCGICNTHGGKGYGFWLKYVKVFFIS NSSNVPCLFYVTIQDNEWNSSLGLHVRRSMF(+) MKTEGILQLLLHRKLWIRGDVAFAIHMVGKDMGSG(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chen et al., 2017b

circRNA

Detail information of Zm00001d045516_circ_g.3

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