Plant Circular RNA Database

Detail information of Os03g0855700_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os03g0855700_circ_g.1
					
						ID in PlantcircBase
						osa_circ_022783
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 36082779-36093748  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0855700						
					
						Parent gene annotation
						
							Nucleic acid-binding, OB-fold domain containing protein. (Os03t0
855700-01);Hypothetical conserved gene. (Os03t0855700-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0855700_circ_g.2 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0855700-01:3

General Information
CircRNA Name	Os03g0855700_circ_g.1
ID in PlantcircBase	osa_circ_022783
Alias	NA
Organism	Oryza sativa
Position	chr3: 36082779-36093748 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0855700
Parent gene annotation	Nucleic acid-binding, OB-fold domain containing protein. (Os03t0 855700-01);Hypothetical conserved gene. (Os03t0855700-02)
Parent gene strand	+
Alternative splicing	Os03g0855700_circ_g.2
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0855700-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAAGATACTGGACCACCATCCACGTTCCCATCTTATGCCTGCAAAATTCTTTCTTTTGATGAAC
TCAGAGCTCGGGCTTACAAGAAAGATATTATTTCAGgctgcactgctggattggggattctctg
ttggcgttttcacttgggggagggagttgcctccctcgtctactgctcgcaaaggtattttgtg
ctctctcg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 500
Transcript exons: 36082779-36082860,36093253-36093393,36093472-36093748
GCTGCACTGCTGGATTGGGGATTCTCTGTTGGCGTTTTCACTTGGGGGAGGGAGTTGCCTCCCT
CGTCTACTGCTCGCAAAGATGGATTACAGTCTCCTAAAGGATGTCACACAGGAATCACACCGTT
GGCGCGTACGAGTGCGGGCAACCCGCTTCTCGGAGTTCACCACAGCTAATGAGCCTGACAAGAT
TTTGCGCCTTGATTTAGTCCTGCTTGATGAGCAGGGCGACATGATGGATGCGCAAATCCCAGGA
CGCCGCGTTGTTCAATTCAAGCCTTTGCTAAAGGAAGGCGCTGTGTATTACATCAAATACTTCG
AAGTCGCCGAGGCACGCCCCCAGTATAGGCCTGTTGATCGACTGTTGATGGCAAAGTTCACTGC
TCACACAACCGTTACAGAAGATACTGGACCACCATCCACGTTCCCATCTTATGCCTGCAAAATT
CTTTCTTTTGATGAACTCAGAGCTCGGGCTTACAAGAAAGATATTATTTCAG
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	GAAGATACTGGACCACCATCCACGTTCCCATCTTATGCCTGCAAAATTCTTTCTTTTGATGAAC TCAGAGCTCGGGCTTACAAGAAAGATATTATTTCAGgctgcactgctggattggggattctctg ttggcgttttcacttgggggagggagttgcctccctcgtctactgctcgcaaaggtattttgtg ctctctcg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 500 Transcript exons: 36082779-36082860,36093253-36093393,36093472-36093748 GCTGCACTGCTGGATTGGGGATTCTCTGTTGGCGTTTTCACTTGGGGGAGGGAGTTGCCTCCCT CGTCTACTGCTCGCAAAGATGGATTACAGTCTCCTAAAGGATGTCACACAGGAATCACACCGTT GGCGCGTACGAGTGCGGGCAACCCGCTTCTCGGAGTTCACCACAGCTAATGAGCCTGACAAGAT TTTGCGCCTTGATTTAGTCCTGCTTGATGAGCAGGGCGACATGATGGATGCGCAAATCCCAGGA CGCCGCGTTGTTCAATTCAAGCCTTTGCTAAAGGAAGGCGCTGTGTATTACATCAAATACTTCG AAGTCGCCGAGGCACGCCCCCAGTATAGGCCTGTTGATCGACTGTTGATGGCAAAGTTCACTGC TCACACAACCGTTACAGAAGATACTGGACCACCATCCACGTTCCCATCTTATGCCTGCAAAATT CTTTCTTTTGATGAACTCAGAGCTCGGGCTTACAAGAAAGATATTATTTCAG
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.191231648

Conservation Information
Conserved circRNAs	NA
PMCS	0.191231648

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							36093254-36093268(+)
36093684-36093344(+)
						
						Potential amino acid sequence
						
							MDYSLLKDVTQESHRWRVRVRATRFSEFTTANEPDKILRLDLVLLDEQGDMMDAQIPGRRVVQF
KPLLKEGAVYYIKYFEVAEARPQYRPVDRLLMAKFTAHTTVTEDTGPPSTFPSYACKILSFDEL
RARAYKKDIISGCTAGLGILCWRFHLGEGVASLVYCSQRWITVS*(+)
MPAKFFLLMNSELGLTRKILFQAALLDWGFSVGVFTWGRELPPSSTARKDGLQSPKGCHTGITP
LARTSAGNPLLGVHHS*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	36093254-36093268(+) 36093684-36093344(+)
Potential amino acid sequence	MDYSLLKDVTQESHRWRVRVRATRFSEFTTANEPDKILRLDLVLLDEQGDMMDAQIPGRRVVQF KPLLKEGAVYYIKYFEVAEARPQYRPVDRLLMAKFTAHTTVTEDTGPPSTFPSYACKILSFDEL RARAYKKDIISGCTAGLGILCWRFHLGEGVASLVYCSQRWITVS(+) MPAKFFLLMNSELGLTRKILFQAALLDWGFSVGVFTWGRELPPSSTARKDGLQSPKGCHTGITP LARTSAGNPLLGVHHS(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0855700_circ_g.1

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