Plant Circular RNA Database

Detail information of Os12g0165000_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os12g0165000_circ_g.8
					
						ID in PlantcircBase
						osa_circ_010576
					
						Alias
						Os_ciR3323
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 3314012-3315647  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os12g0165000						
					
						Parent gene annotation
						
							WD40 repeat-like domain containing protein. (Os12t0165000-01);Si
milar to predicted protein. (Os12t0165000-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os12g0165000_circ_g.3 Os12g0165000_circ_g.4 Os12g0165000_circ_g.5 Os12g0165000_circ_g.6 Os12g0165000_circ_g.7 Os12g0165000_circ_g.9 Os12g0165000_circ_g.10 						
					
						Support reads
						3/1
					
						Tissues
						root/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0165000-01:6

General Information
CircRNA Name	Os12g0165000_circ_g.8
ID in PlantcircBase	osa_circ_010576
Alias	Os_ciR3323
Organism	Oryza sativa
Position	chr12: 3314012-3315647 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os12g0165000
Parent gene annotation	WD40 repeat-like domain containing protein. (Os12t0165000-01);Si milar to predicted protein. (Os12t0165000-02)
Parent gene strand	-
Alternative splicing	Os12g0165000_circ_g.3 Os12g0165000_circ_g.4 Os12g0165000_circ_g.5 Os12g0165000_circ_g.6 Os12g0165000_circ_g.7 Os12g0165000_circ_g.9 Os12g0165000_circ_g.10
Support reads	3/1
Tissues	root/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0165000-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCTCCTTTCTCGGTCGACTTGAACCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAG
ATGCCTAATTTATCTAAAACCGGAGCAGCTTATAGCctgccagtaggatcaagaagtaaagccc
tgatattgtcagtgtgccctctcaatttcattttctttgaaccagtcctgggatcccaaacgcg
aacaacct

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 845
Transcript exons: 3314012-3314109,3314248-3314541,3314630-3314719,3314792-
3314854,3315230-3315388,3315507-3315647
CTGCCAGTAGGATCAAGAAGTAAAGCCCTGATATTGTCAGTGTGCCCTCTCAATTTCATTTTCT
TTGAACCAGTCCTGGGATCCCAAACGCGAACAACCTTTTCAGTACCACCAGAGACAAGTGTGTT
TCCTGTGTCACTCATGGCCAATGCATAAACTGAATCTTTGTGACCTTTGGCAGTGATTGGACTG
TACCCATGTGATTGCCCATTGGTGGAAGCAAGGTTGCTGCTAGAATTTACATTGCACAAGGTTG
TCAAAGCAGGCCCAGAGTTTCCATTAGGAGCTTCATCCTCTTTTGCATCTACAGATTTGGCCAC
AGGAGCAAGAGAAGAATCAAGGTCCCATATAAAGACCTCACCGCCAAGGCCACCAGAGGCAACA
ATATTGCTATTTTTTTCAGATGCAGCAAGACAAATCACATAATCGGAATGCTGGCGGAGAGTCC
TGGTACAAGCACCATCTGATAAACAATTCCACACCTTTAGAGTGGTATCTGAGGAACAGGAAAC
AAGATTTTGGCCAACAATAATGGCATCATTAACCCAATCAACATGTGACTCAAAAGTTGCTGAG
AAGCTTGCGACTCCATTTTTTGGTTCCCACCTCTTCAAGGTACCATCCCGACTCCCGGTAAAAA
GATAATCACTCGTACCAGAAGTAGAGGCATTTAGGTATGACAAGCAGTTGATCCCAGCACAATG
CTTTTTGTTATCAGCGTCATTCAGCACATATGTAAAGCGCTTCTCCTTTCTCGGTCGACTTGAA
CCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAGATGCCTAATTTATCTAAAACCGG
AGCAGCTTATAGC
						
					

						Genomic sequence
						
							CTGCCAGTAGGATCAAGAAGTAAAGCCCTGATATTGTCAGTGTGCCCTCTCAATTTCATTTTCT
TTGAACCAGTCCTGGGATCCCAAACGCGAACAACCTACACTAAACAGGTACATATAAGGAAATA
TCACAGGAGAGGTCTCTCAAAAGCAGTCCAAAGAAATTCGCACATTATGGTGCCTCCTAACCCC
AAGGAAACTTGCATACTCATTCTCAAAGATGAAGAACATAATACCTTTTCAGTACCACCAGAGA
CAAGTGTGTTTCCTGTGTCACTCATGGCCAATGCATAAACTGAATCTTTGTGACCTTTGGCAGT
GATTGGACTGTACCCATGTGATTGCCCATTGGTGGAAGCAAGGTTGCTGCTAGAATTTACATTG
CACAAGGTTGTCAAAGCAGGCCCAGAGTTTCCATTAGGAGCTTCATCCTCTTTTGCATCTACAG
ATTTGGCCACAGGAGCAAGAGAAGAATCAAGGTCCCATATAAAGACCTCACCGCCAAGGCCACC
AGAGGCAACAATATTGCTCTACATTGCAGAGAATAAAAAAAAACATGTAAAACAAAAGGGATAA
AATGAGTAGTTCCACAGAAAAGCATAGTGCTGGATAATATACATTTTTTTCAGATGCAGCAAGA
CAAATCACATAATCGGAATGCTGGCGGAGAGTCCTGGTACAAGCACCATCTGATAAACAATTCC
ACACCTGTGAAACAATTAGGAATTAAAAAACATGTGGATCCAAAATGAATATATAAAAACTGCT
GAAACAACAGACCTTTAGAGTGGTATCTGAGGAACAGGAAACAAGATTTTGGCCAACAATAATG
GCATCATTAACCTGCATGATAAATATATCAGCTTAAATCATAGAAAGTACTTCAGAGATAATGA
AAGCGTCCATGTTACAAAATTAAGCTCAAATCTCCTCAAGGATGAGTTTCTCCATCAATGATAG
AATTCCATCCGAAACTATAATACTTGGATATTGAAAACTATGTAGTCTTCTTTCCATCTGATCC
TCTTTTGTTTTGGGGCAAGATGCTTATACATCTTTATTCAAATAAAAGTGGCAATGGATCATCA
AAAAACACGATAATGGATAAATGTTGCTATGGTATGCGTCATCATGTAACTTTGGGCTAGTAAA
ATTTCAAAACGTAATGGTCAAATAACAATATTAAATCGACTGGAGTAAAAACAGAGAAACGCAT
ACCCAATCAACATGTGACTCAAAAGTTGCTGAGAAGCTTGCGACTCCATTTTTTGGTTCCCACC
TCTTCAAGGTACCATCCCGACTCCCGGTAAAAAGATAATCACTCGTACCAGAAGTAGAGGCATT
TAGGTATGACAAGCAGTTGATCCCAGCACAATGCTGAAAAATAGACACAATGGAATGCATGATG
AGACATTTGCACTGAAAATGAATTCTTTTGAATCAACTGGATGCATCCGCGATTCTGAGGATAG
CTAGTTCTGGAAGTGGAACTGACCTTTTTGTTATCAGCGTCATTCAGCACATATGTAAAGCGCT
TCTCCTTTCTCGGTCGACTTGAACCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAG
ATGCCTAATTTATCTAAAACCGGAGCAGCTTATAGC
						
					

			
		

Sequences
Splice junction sequence	TCTCCTTTCTCGGTCGACTTGAACCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAG ATGCCTAATTTATCTAAAACCGGAGCAGCTTATAGCctgccagtaggatcaagaagtaaagccc tgatattgtcagtgtgccctctcaatttcattttctttgaaccagtcctgggatcccaaacgcg aacaacct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 845 Transcript exons: 3314012-3314109,3314248-3314541,3314630-3314719,3314792- 3314854,3315230-3315388,3315507-3315647 CTGCCAGTAGGATCAAGAAGTAAAGCCCTGATATTGTCAGTGTGCCCTCTCAATTTCATTTTCT TTGAACCAGTCCTGGGATCCCAAACGCGAACAACCTTTTCAGTACCACCAGAGACAAGTGTGTT TCCTGTGTCACTCATGGCCAATGCATAAACTGAATCTTTGTGACCTTTGGCAGTGATTGGACTG TACCCATGTGATTGCCCATTGGTGGAAGCAAGGTTGCTGCTAGAATTTACATTGCACAAGGTTG TCAAAGCAGGCCCAGAGTTTCCATTAGGAGCTTCATCCTCTTTTGCATCTACAGATTTGGCCAC AGGAGCAAGAGAAGAATCAAGGTCCCATATAAAGACCTCACCGCCAAGGCCACCAGAGGCAACA ATATTGCTATTTTTTTCAGATGCAGCAAGACAAATCACATAATCGGAATGCTGGCGGAGAGTCC TGGTACAAGCACCATCTGATAAACAATTCCACACCTTTAGAGTGGTATCTGAGGAACAGGAAAC AAGATTTTGGCCAACAATAATGGCATCATTAACCCAATCAACATGTGACTCAAAAGTTGCTGAG AAGCTTGCGACTCCATTTTTTGGTTCCCACCTCTTCAAGGTACCATCCCGACTCCCGGTAAAAA GATAATCACTCGTACCAGAAGTAGAGGCATTTAGGTATGACAAGCAGTTGATCCCAGCACAATG CTTTTTGTTATCAGCGTCATTCAGCACATATGTAAAGCGCTTCTCCTTTCTCGGTCGACTTGAA CCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAGATGCCTAATTTATCTAAAACCGG AGCAGCTTATAGC
Genomic sequence	CTGCCAGTAGGATCAAGAAGTAAAGCCCTGATATTGTCAGTGTGCCCTCTCAATTTCATTTTCT TTGAACCAGTCCTGGGATCCCAAACGCGAACAACCTACACTAAACAGGTACATATAAGGAAATA TCACAGGAGAGGTCTCTCAAAAGCAGTCCAAAGAAATTCGCACATTATGGTGCCTCCTAACCCC AAGGAAACTTGCATACTCATTCTCAAAGATGAAGAACATAATACCTTTTCAGTACCACCAGAGA CAAGTGTGTTTCCTGTGTCACTCATGGCCAATGCATAAACTGAATCTTTGTGACCTTTGGCAGT GATTGGACTGTACCCATGTGATTGCCCATTGGTGGAAGCAAGGTTGCTGCTAGAATTTACATTG CACAAGGTTGTCAAAGCAGGCCCAGAGTTTCCATTAGGAGCTTCATCCTCTTTTGCATCTACAG ATTTGGCCACAGGAGCAAGAGAAGAATCAAGGTCCCATATAAAGACCTCACCGCCAAGGCCACC AGAGGCAACAATATTGCTCTACATTGCAGAGAATAAAAAAAAACATGTAAAACAAAAGGGATAA AATGAGTAGTTCCACAGAAAAGCATAGTGCTGGATAATATACATTTTTTTCAGATGCAGCAAGA CAAATCACATAATCGGAATGCTGGCGGAGAGTCCTGGTACAAGCACCATCTGATAAACAATTCC ACACCTGTGAAACAATTAGGAATTAAAAAACATGTGGATCCAAAATGAATATATAAAAACTGCT GAAACAACAGACCTTTAGAGTGGTATCTGAGGAACAGGAAACAAGATTTTGGCCAACAATAATG GCATCATTAACCTGCATGATAAATATATCAGCTTAAATCATAGAAAGTACTTCAGAGATAATGA AAGCGTCCATGTTACAAAATTAAGCTCAAATCTCCTCAAGGATGAGTTTCTCCATCAATGATAG AATTCCATCCGAAACTATAATACTTGGATATTGAAAACTATGTAGTCTTCTTTCCATCTGATCC TCTTTTGTTTTGGGGCAAGATGCTTATACATCTTTATTCAAATAAAAGTGGCAATGGATCATCA AAAAACACGATAATGGATAAATGTTGCTATGGTATGCGTCATCATGTAACTTTGGGCTAGTAAA ATTTCAAAACGTAATGGTCAAATAACAATATTAAATCGACTGGAGTAAAAACAGAGAAACGCAT ACCCAATCAACATGTGACTCAAAAGTTGCTGAGAAGCTTGCGACTCCATTTTTTGGTTCCCACC TCTTCAAGGTACCATCCCGACTCCCGGTAAAAAGATAATCACTCGTACCAGAAGTAGAGGCATT TAGGTATGACAAGCAGTTGATCCCAGCACAATGCTGAAAAATAGACACAATGGAATGCATGATG AGACATTTGCACTGAAAATGAATTCTTTTGAATCAACTGGATGCATCCGCGATTCTGAGGATAG CTAGTTCTGGAAGTGGAACTGACCTTTTTGTTATCAGCGTCATTCAGCACATATGTAAAGCGCT TCTCCTTTCTCGGTCGACTTGAACCTGCCGTGTTACCAGCACTTCCAACACGATGCATTGAAAG ATGCCTAATTTATCTAAAACCGGAGCAGCTTATAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_003303* osi_circ_010116 zma_circ_007664 						
					
						PMCS
						0.366938809

Conservation Information
Conserved circRNAs	osi_circ_003303* osi_circ_010116 zma_circ_007664
PMCS	0.366938809

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3315613-3314302(+)
3315607-3315643(-)
						
						Potential amino acid sequence
						
							MPNLSKTGAAYSLPVGSRSKALILSVCPLNFIFFEPVLGSQTRTTFSVPPETSVFPVSLMANA*
(+)
MHRVGSAGNTAGSSRPRKEKRFTYVLNDADNKKHCAGINCLSYLNASTSGTSDYLFTGSRDGTL
KRWEPKNGVASFSATFESHVDWVNDAIIVGQNLVSCSSDTTLKVWNCLSDGACTRTLRQHSDYV
ICLAASEKNSNIVASGGLGGEVFIWDLDSSLAPVAKSVDAKEDEAPNGNSGPALTTLCNVNSSS
NLASTNGQSHGYSPITAKGHKDSVYALAMSDTGNTLVSGGTEKVVRVWDPRTGSKKMKLRGHTD
NIRALLLDPTGRL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3315613-3314302(+) 3315607-3315643(-)
Potential amino acid sequence	MPNLSKTGAAYSLPVGSRSKALILSVCPLNFIFFEPVLGSQTRTTFSVPPETSVFPVSLMANA* (+) MHRVGSAGNTAGSSRPRKEKRFTYVLNDADNKKHCAGINCLSYLNASTSGTSDYLFTGSRDGTL KRWEPKNGVASFSATFESHVDWVNDAIIVGQNLVSCSSDTTLKVWNCLSDGACTRTLRQHSDYV ICLAASEKNSNIVASGGLGGEVFIWDLDSSLAPVAKSVDAKEDEAPNGNSGPALTTLCNVNSSS NLASTNGQSHGYSPITAKGHKDSVYALAMSDTGNTLVSGGTEKVVRVWDPRTGSKKMKLRGHTD NIRALLLDPTGRL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os12g0165000_circ_g.8

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