Plant Circular RNA Database

Detail information of Os08g0135900_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os08g0135900_circ_g.4
					
						ID in PlantcircBase
						osa_circ_035761
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 2031295-2031737  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0135900						
					
						Parent gene annotation
						
							Similar to Tryptophan synthase beta chain 1 (EC 4.2.1.20) (Orang
e pericarp 1) (Fragment). (Os08t0135900-01);Similar to Tryptopha
n synthase beta-subunit. (Os08t0135900-02);Similar to Tryptophan
 synthase beta-subunit. (Os08t0135900-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os08g0135900_circ_g.1 Os08g0135900_circ_g.2 Os08g0135900_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0136001-00:1
Os08t0135900-01:1
Os08t0135900-03:1
Os08t0136001-00:1
Os08t0135900-02:1

General Information
CircRNA Name	Os08g0135900_circ_g.4
ID in PlantcircBase	osa_circ_035761
Alias	NA
Organism	Oryza sativa
Position	chr8: 2031295-2031737 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0135900
Parent gene annotation	Similar to Tryptophan synthase beta chain 1 (EC 4.2.1.20) (Orang e pericarp 1) (Fragment). (Os08t0135900-01);Similar to Tryptopha n synthase beta-subunit. (Os08t0135900-02);Similar to Tryptophan synthase beta-subunit. (Os08t0135900-03)
Parent gene strand	+
Alternative splicing	Os08g0135900_circ_g.1 Os08g0135900_circ_g.2 Os08g0135900_circ_g.3
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0136001-00:1 Os08t0135900-01:1 Os08t0135900-03:1 Os08t0136001-00:1 Os08t0135900-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTGAGCTATGTGTTGCAGGATGATGA
TGGACAAGTTATTGAACCCCACTCCATTAGTGCTGGgtgagggcagtacattctgggacagcaa
ctttgaaggatgccacctcggaggccatccgtgactgggtcaccaatgttgagaacactcacta
cattttgg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 443
Transcript exons: 2031295-2031737
GTGAGGGCAGTACATTCTGGGACAGCAACTTTGAAGGATGCCACCTCGGAGGCCATCCGTGACT
GGGTCACCAATGTTGAGAACACTCACTACATTTTGGGATCAGTTGCTGGTCCGCATCCATACCC
GATGATGGTGAGAGAGTTCCATAAGGTGATTGGCAAGGAGACCCGTAGACAGGCCATGGAGAAG
TGGGGTGGCAAGCCTGATGTCTTGGTTGCTTGTGTTGGTGGTGGATCAAACGCCATGGGTCTCT
TCCATGAGTTCGTTGATGATCAAGATATAAGAATGATTGGAGTGGAGGCTGCTGGCTATGGTGT
AGACACTGACAAACATGCTGCCACTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTG
AGCTATGTGTTGCAGGATGATGATGGACAAGTTATTGAACCCCACTCCATTAGTGCTGG
						
						Genomic sequence
						
							GTGAGGGCAGTACATTCTGGGACAGCAACTTTGAAGGATGCCACCTCGGAGGCCATCCGTGACT
GGGTCACCAATGTTGAGAACACTCACTACATTTTGGGATCAGTTGCTGGTCCGCATCCATACCC
GATGATGGTGAGAGAGTTCCATAAGGTGATTGGCAAGGAGACCCGTAGACAGGCCATGGAGAAG
TGGGGTGGCAAGCCTGATGTCTTGGTTGCTTGTGTTGGTGGTGGATCAAACGCCATGGGTCTCT
TCCATGAGTTCGTTGATGATCAAGATATAAGAATGATTGGAGTGGAGGCTGCTGGCTATGGTGT
AGACACTGACAAACATGCTGCCACTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTG
AGCTATGTGTTGCAGGATGATGATGGACAAGTTATTGAACCCCACTCCATTAGTGCTGG

Sequences
Splice junction sequence	CTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTGAGCTATGTGTTGCAGGATGATGA TGGACAAGTTATTGAACCCCACTCCATTAGTGCTGGgtgagggcagtacattctgggacagcaa ctttgaaggatgccacctcggaggccatccgtgactgggtcaccaatgttgagaacactcacta cattttgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 443 Transcript exons: 2031295-2031737 GTGAGGGCAGTACATTCTGGGACAGCAACTTTGAAGGATGCCACCTCGGAGGCCATCCGTGACT GGGTCACCAATGTTGAGAACACTCACTACATTTTGGGATCAGTTGCTGGTCCGCATCCATACCC GATGATGGTGAGAGAGTTCCATAAGGTGATTGGCAAGGAGACCCGTAGACAGGCCATGGAGAAG TGGGGTGGCAAGCCTGATGTCTTGGTTGCTTGTGTTGGTGGTGGATCAAACGCCATGGGTCTCT TCCATGAGTTCGTTGATGATCAAGATATAAGAATGATTGGAGTGGAGGCTGCTGGCTATGGTGT AGACACTGACAAACATGCTGCCACTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTG AGCTATGTGTTGCAGGATGATGATGGACAAGTTATTGAACCCCACTCCATTAGTGCTGG
Genomic sequence	GTGAGGGCAGTACATTCTGGGACAGCAACTTTGAAGGATGCCACCTCGGAGGCCATCCGTGACT GGGTCACCAATGTTGAGAACACTCACTACATTTTGGGATCAGTTGCTGGTCCGCATCCATACCC GATGATGGTGAGAGAGTTCCATAAGGTGATTGGCAAGGAGACCCGTAGACAGGCCATGGAGAAG TGGGGTGGCAAGCCTGATGTCTTGGTTGCTTGTGTTGGTGGTGGATCAAACGCCATGGGTCTCT TCCATGAGTTCGTTGATGATCAAGATATAAGAATGATTGGAGTGGAGGCTGCTGGCTATGGTGT AGACACTGACAAACATGCTGCCACTTTGACAAAGGGAGAAGTGGGAGTTCTCCATGGATCATTG AGCTATGTGTTGCAGGATGATGATGGACAAGTTATTGAACCCCACTCCATTAGTGCTGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.724940835

Conservation Information
Conserved circRNAs	NA
PMCS	0.724940835

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2031425-2031295(+)
2031684-2031353(+)
2031352-2031730(-)
2031311-2031683(-)
						
						Potential amino acid sequence
						
							MMVREFHKVIGKETRRQAMEKWGGKPDVLVACVGGGSNAMGLFHEFVDDQDIRMIGVEAAGYGV
DTDKHAATLTKGEVGVLHGSLSYVLQDDDGQVIEPHSISAG*(+)
MCCRMMMDKLLNPTPLVLGEGSTFWDSNFEGCHLGGHP*(+)
MASEVASFKVAVPECTALTQH*(-)
MYCPHPALMEWGSITCPSSSCNT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2031425-2031295(+) 2031684-2031353(+) 2031352-2031730(-) 2031311-2031683(-)
Potential amino acid sequence	MMVREFHKVIGKETRRQAMEKWGGKPDVLVACVGGGSNAMGLFHEFVDDQDIRMIGVEAAGYGV DTDKHAATLTKGEVGVLHGSLSYVLQDDDGQVIEPHSISAG(+) MCCRMMMDKLLNPTPLVLGEGSTFWDSNFEGCHLGGHP(+) MASEVASFKVAVPECTALTQH(-) MYCPHPALMEWGSITCPSSSCNT(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0135900_circ_g.4

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