Plant Circular RNA Database

Detail information of Os03g0221500_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os03g0221500_circ_g.1
					
						ID in PlantcircBase
						osa_circ_018571
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 6371767-6373132  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0221500						
					
						Parent gene annotation
						
							Glycoside hydrolase, family 17 protein. (Os03t0221500-01);Glycos
ide hydrolase, family 17 protein. (Os03t0221500-02);Glycoside hy
drolase, family 17 protein. (Os03t0221500-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0221500_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0221500-02:2
Os03t0221500-03:2
Os03t0221500-01:2

General Information
CircRNA Name	Os03g0221500_circ_g.1
ID in PlantcircBase	osa_circ_018571
Alias	NA
Organism	Oryza sativa
Position	chr3: 6371767-6373132 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0221500
Parent gene annotation	Glycoside hydrolase, family 17 protein. (Os03t0221500-01);Glycos ide hydrolase, family 17 protein. (Os03t0221500-02);Glycoside hy drolase, family 17 protein. (Os03t0221500-03)
Parent gene strand	+
Alternative splicing	Os03g0221500_circ_g.2
Support reads	2
Tissues	shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0221500-02:2 Os03t0221500-03:2 Os03t0221500-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAAACCATAAGAGATTCCTGCTTTCCAAACATTTGGATTTGAGCTTATTTCGTTCTCTCTGTT
TTCTTCAGGCCACGGGTCTTGCGTTTACGCTGGAAGgggcgtacatcggcgtcaacattggcac
ggccatgacgtcggtaccggcgccgacacagatcacgacgctgctccgctcgcagaacatccgc
cacgtgcg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GGGCGTACATCGGCGTCAACATTGGCACGGCCATGACGTCGGTACCGGCGCCGACACAGATCAC
GACGCTGCTCCGCTCGCAGAACATCCGCCACGTGCGGCTGTACGACGCCGACCCGGCGATGCTC
GCGGCGCTCGCCAACACCGGCATCCGCGTCATCGTCTCCGTGCCGAACGAGCAGCTCCTCGCCA
TCGGCAACTCCAACGCCACGGCGGCCAACTGGGTGGCGCGCAACGTGGCCGCGCACTACCCTTC
CGTGAACATCACGGCCATCGCGGTCGGCTCCGAGGTGCTCTCCACGCTGCCCAACGCGGCGCCG
CTCCTCATGCCGGCCATCCGCTACCTCCAGAACGCGCTGGTGGCCGCGGCGCTCGACCGGTACA
TCAAGATCTCGACGCCGCACTCCTCGTCCATCATCCTCGACTCGTTCCCGCCGTCGCAGGCCTT
CTTCAACCGCTCCCTGGACCCGGTGCTGGTGCCGCTGCTCAAGTTCTTGCAGTCCACGGGCTCG
CCGCTGATGCTCAACGTGTACCCGTACTACGACTACATGCGCTCCAACGGCGTCATCCCGCTGG
ACTACGCGCTGTTCCGGCCGCTGCCGCCGAACAAGGAGGCGGTGGACGCCAACACCCTGCTGCA
CTACACCAACGTGTTCGACGCGGTCGTGGACGCTGCCTACTTCGCCATGGCGTACCTCAACGTC
ACCAACGTGCCGGTGATGGTGACGGAGACCGGGTGGCCGCACAAGGGCGACCCGTCCAACGAGC
CCGACGCCACCTCCGACAACGCCGACACCTACAACAGTAACCTCATCCGGCACGTGATGAACAC
CACCGGCACGCCGAAGCACCCCGGCGTCGCCGTGCCGACGTACATCTACGAGCTCTACGACGAG
GACACCCGCCCCGGCTCGACGTCGGAGAAGTACTGGGGCCTGTTCGACATGAACGGCATCCCGG
CGTACACCCTGCACCTGACAGGCTCCGGCGTGCTGCTCGCCAACGACACGACGAACCAGACCTA
CTGCGTGGCGCGGGAGGGCGCTGACGAGAAGATGCTGCAGGCGGCGCTCGACTGGGCTTGCGGC
CCGGGCAAGGTGGACTGCTCCGCGCTGATGCAGGGGCAGCCGTGCTACGACCCGGACAACGTCG
AGGCGCACGCCACCTACGCCTTCAACGCCTACTACCATGGCATGGGCATGGGCTCCGGGACGTG
CTACTTCAGCGGTGTCGCCGTGATCACCACCACCGATCCCAGTAAGTTCACAAAACCATAAGAG
ATTCCTGCTTTCCAAACATTTGGATTTGAGCTTATTTCGTTCTCTCTGTTTTCTTCAGGCCACG
GGTCTTGCGTTTACGCTGGAAG

Sequences
Splice junction sequence	CAAAACCATAAGAGATTCCTGCTTTCCAAACATTTGGATTTGAGCTTATTTCGTTCTCTCTGTT TTCTTCAGGCCACGGGTCTTGCGTTTACGCTGGAAGgggcgtacatcggcgtcaacattggcac ggccatgacgtcggtaccggcgccgacacagatcacgacgctgctccgctcgcagaacatccgc cacgtgcg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGGCGTACATCGGCGTCAACATTGGCACGGCCATGACGTCGGTACCGGCGCCGACACAGATCAC GACGCTGCTCCGCTCGCAGAACATCCGCCACGTGCGGCTGTACGACGCCGACCCGGCGATGCTC GCGGCGCTCGCCAACACCGGCATCCGCGTCATCGTCTCCGTGCCGAACGAGCAGCTCCTCGCCA TCGGCAACTCCAACGCCACGGCGGCCAACTGGGTGGCGCGCAACGTGGCCGCGCACTACCCTTC CGTGAACATCACGGCCATCGCGGTCGGCTCCGAGGTGCTCTCCACGCTGCCCAACGCGGCGCCG CTCCTCATGCCGGCCATCCGCTACCTCCAGAACGCGCTGGTGGCCGCGGCGCTCGACCGGTACA TCAAGATCTCGACGCCGCACTCCTCGTCCATCATCCTCGACTCGTTCCCGCCGTCGCAGGCCTT CTTCAACCGCTCCCTGGACCCGGTGCTGGTGCCGCTGCTCAAGTTCTTGCAGTCCACGGGCTCG CCGCTGATGCTCAACGTGTACCCGTACTACGACTACATGCGCTCCAACGGCGTCATCCCGCTGG ACTACGCGCTGTTCCGGCCGCTGCCGCCGAACAAGGAGGCGGTGGACGCCAACACCCTGCTGCA CTACACCAACGTGTTCGACGCGGTCGTGGACGCTGCCTACTTCGCCATGGCGTACCTCAACGTC ACCAACGTGCCGGTGATGGTGACGGAGACCGGGTGGCCGCACAAGGGCGACCCGTCCAACGAGC CCGACGCCACCTCCGACAACGCCGACACCTACAACAGTAACCTCATCCGGCACGTGATGAACAC CACCGGCACGCCGAAGCACCCCGGCGTCGCCGTGCCGACGTACATCTACGAGCTCTACGACGAG GACACCCGCCCCGGCTCGACGTCGGAGAAGTACTGGGGCCTGTTCGACATGAACGGCATCCCGG CGTACACCCTGCACCTGACAGGCTCCGGCGTGCTGCTCGCCAACGACACGACGAACCAGACCTA CTGCGTGGCGCGGGAGGGCGCTGACGAGAAGATGCTGCAGGCGGCGCTCGACTGGGCTTGCGGC CCGGGCAAGGTGGACTGCTCCGCGCTGATGCAGGGGCAGCCGTGCTACGACCCGGACAACGTCG AGGCGCACGCCACCTACGCCTTCAACGCCTACTACCATGGCATGGGCATGGGCTCCGGGACGTG CTACTTCAGCGGTGTCGCCGTGATCACCACCACCGATCCCAGTAAGTTCACAAAACCATAAGAG ATTCCTGCTTTCCAAACATTTGGATTTGAGCTTATTTCGTTCTCTCTGTTTTCTTCAGGCCACG GGTCTTGCGTTTACGCTGGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_014070 						
					
						PMCS
						0.305887622

Conservation Information
Conserved circRNAs	osi_circ_014070
PMCS	0.305887622

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							6371800-6372571(+)
6371961-6373008(-)
6372958-6373008(-)
						
					

						Potential amino acid sequence
						
							MTSVPAPTQITTLLRSQNIRHVRLYDADPAMLAALANTGIRVIVSVPNEQLLAIGNSNATAANW
VARNVAAHYPSVNITAIAVGSEVLSTLPNAAPLLMPAIRYLQNALVAAALDRYIKISTPHSSSI
ILDSFPPSQAFFNRSLDPVLVPLLKFLQSTGSPLMLNVYPYYDYMRSNGVIPLDYALFRPLPPN
KEAVDANTLLHYTNVFDAVVDAAYFAMAYLNVTNVPVMVTETGWPHKGDPSNEPDATSDNADTY
NSNLIRHVMNTTGTPKHPGVAVPTYIYELYDEDTRPGSTSEKYWGLFDMNGIPAYTLHLTGSGV
LLANDTTNQTYCVAREGADEKMLQAALDWACGPGKVDCSALMQGQPCYDPDNVEAHATYAFNAY
YHGMGMGSGTCYFSGVAVITTTDPSHGSCVYAGRGVHRRQHWHGHDVGTGADTDHDAAPLAEHP
PRAAVRRRPGDARGARQHRHPRHRLRAERAAPRHRQLQRHGGQLGGAQRGRALPFREHHGHRGR
LRGALHAAQRGAAPHAGHPLPPERAGGRGARPVHQDLDAALLVHHPRLVPAVAGLLQPLPGPGA
GAAAQVLAVHGLAADAQRVPVLRLHALQRRHPAGLRAVPAAAAEQGGGGRQHPAALHQRVRRGR
GRCLLRHGVPQRHQRAGDGDGDRVAAQGRPVQRARRHLRQRRHLQQ*(+)
MARSCSFGTETMTRMPVLASAASIAGSASYSRTWRMFCERSSVVICVGAGTDVMAVPMLTPMYA
PSSVNARPVAGIGGGDHGDTAEVARPGAHAHAMVVGVEGVGGVRLDVVRVVARLPLHQRGAVHL
ARAASPVERRLQHLLVSALPRHAVGLVRRVVGEQHAGACQVQGVRRDAVHVEQAPVLLRRRAGA
GVLVVELVDVRRHGDAGVLRRAGGVHHVPDEVTVVGVGVVGGGVGLVGRVALVRPPGLRHHHRH
VGDVEVRHGEVGSVHDRVEHVGVVQQGVGVHRLLVRRQRPEQRVVQRDDAVGAHVVVVRVHVEH
QRRARGLQELEQRHQHRVQGAVEEGLRRRERVEDDGRGVRRRDLDVPVERRGHQRVLEVADGRH
EERRRVGQRGEHLGADRDGRDVHGRVVRGHVARHPVGRRGVGVADGEELLVRHGDDDADAGVGE
RREHRRVGVVQPHVADVLRAEQRRDLCRRRYRRHGRANVDADVRPFQRKRKTRGWDRWW*(-)
MVVGVEGVGGVRLDVVRVVARLPLHQRGAVHLARAASPVERRLQHLLVSALPRHAVGLVRRVVG
EQHAGACQVQGVRRDAVHVEQAPVLLRRRAGAGVLVVELVDVRRHGDAGVLRRAGGVHHVPDEV
TVVGVGVVGGGVGLVGRVALVRPPGLRHHHRHVGDVEVRHGEVGSVHDRVEHVGVVQQGVGVHR
LLVRRQRPEQRVVQRDDAVGAHVVVVRVHVEHQRRARGLQELEQRHQHRVQGAVEEGLRRRERV
EDDGRGVRRRDLDVPVERRGHQRVLEVADGRHEERRRVGQRGEHLGADRDGRDVHGRVVRGHVA
RHPVGRRGVGVADGEELLVRHGDDDADAGVGERREHRRVGVVQPHVADVLRAEQRRDLCRRRYR
RHGRANVDADVRPFQRKRKTRGWDRWW*(-)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	6371800-6372571(+) 6371961-6373008(-) 6372958-6373008(-)
Potential amino acid sequence	MTSVPAPTQITTLLRSQNIRHVRLYDADPAMLAALANTGIRVIVSVPNEQLLAIGNSNATAANW VARNVAAHYPSVNITAIAVGSEVLSTLPNAAPLLMPAIRYLQNALVAAALDRYIKISTPHSSSI ILDSFPPSQAFFNRSLDPVLVPLLKFLQSTGSPLMLNVYPYYDYMRSNGVIPLDYALFRPLPPN KEAVDANTLLHYTNVFDAVVDAAYFAMAYLNVTNVPVMVTETGWPHKGDPSNEPDATSDNADTY NSNLIRHVMNTTGTPKHPGVAVPTYIYELYDEDTRPGSTSEKYWGLFDMNGIPAYTLHLTGSGV LLANDTTNQTYCVAREGADEKMLQAALDWACGPGKVDCSALMQGQPCYDPDNVEAHATYAFNAY YHGMGMGSGTCYFSGVAVITTTDPSHGSCVYAGRGVHRRQHWHGHDVGTGADTDHDAAPLAEHP PRAAVRRRPGDARGARQHRHPRHRLRAERAAPRHRQLQRHGGQLGGAQRGRALPFREHHGHRGR LRGALHAAQRGAAPHAGHPLPPERAGGRGARPVHQDLDAALLVHHPRLVPAVAGLLQPLPGPGA GAAAQVLAVHGLAADAQRVPVLRLHALQRRHPAGLRAVPAAAAEQGGGGRQHPAALHQRVRRGR GRCLLRHGVPQRHQRAGDGDGDRVAAQGRPVQRARRHLRQRRHLQQ(+) MARSCSFGTETMTRMPVLASAASIAGSASYSRTWRMFCERSSVVICVGAGTDVMAVPMLTPMYA PSSVNARPVAGIGGGDHGDTAEVARPGAHAHAMVVGVEGVGGVRLDVVRVVARLPLHQRGAVHL ARAASPVERRLQHLLVSALPRHAVGLVRRVVGEQHAGACQVQGVRRDAVHVEQAPVLLRRRAGA GVLVVELVDVRRHGDAGVLRRAGGVHHVPDEVTVVGVGVVGGGVGLVGRVALVRPPGLRHHHRH VGDVEVRHGEVGSVHDRVEHVGVVQQGVGVHRLLVRRQRPEQRVVQRDDAVGAHVVVVRVHVEH QRRARGLQELEQRHQHRVQGAVEEGLRRRERVEDDGRGVRRRDLDVPVERRGHQRVLEVADGRH EERRRVGQRGEHLGADRDGRDVHGRVVRGHVARHPVGRRGVGVADGEELLVRHGDDDADAGVGE RREHRRVGVVQPHVADVLRAEQRRDLCRRRYRRHGRANVDADVRPFQRKRKTRGWDRWW(-) MVVGVEGVGGVRLDVVRVVARLPLHQRGAVHLARAASPVERRLQHLLVSALPRHAVGLVRRVVG EQHAGACQVQGVRRDAVHVEQAPVLLRRRAGAGVLVVELVDVRRHGDAGVLRRAGGVHHVPDEV TVVGVGVVGGGVGLVGRVALVRPPGLRHHHRHVGDVEVRHGEVGSVHDRVEHVGVVQQGVGVHR LLVRRQRPEQRVVQRDDAVGAHVVVVRVHVEHQRRARGLQELEQRHQHRVQGAVEEGLRRRERV EDDGRGVRRRDLDVPVERRGHQRVLEVADGRHEERRRVGQRGEHLGADRDGRDVHGRVVRGHVA RHPVGRRGVGVADGEELLVRHGDDDADAGVGERREHRRVGVVQPHVADVLRAEQRRDLCRRRYR RHGRANVDADVRPFQRKRKTRGWDRWW*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0221500_circ_g.1

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