Plant Circular RNA Database

Detail information of AT3G29185_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT3G29185_circ_g.5
					
						ID in PlantcircBase
						ath_circ_024302
					
						Alias
						At_ciR3377, AT3G29185_C2
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 11155468-11155611  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT3G29185						
					
						Parent gene annotation
						
							Glutamate NMDA receptor subunit epsilon-1, putative (DUF3598)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT3G29185_circ_g.1 AT3G29185_circ_g.2 AT3G29185_circ_g.3 AT3G29185_circ_g.4 AT3G29185_circ_g.6 AT3G29185_circ_g.7 AT3G29185_circ_g.8 AT3G29185_circ_g.9 AT3G29185_circ_g.10 						
					
						Support reads
						2/9
					
						Tissues
						leaf/aerial, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G29185.2:1
AT3G29185.1:1

General Information
CircRNA Name	AT3G29185_circ_g.5
ID in PlantcircBase	ath_circ_024302
Alias	At_ciR3377, AT3G29185_C2
Organism	Arabidpsis thaliana
Position	chr3: 11155468-11155611 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT3G29185
Parent gene annotation	Glutamate NMDA receptor subunit epsilon-1, putative (DUF3598)
Parent gene strand	-
Alternative splicing	AT3G29185_circ_g.1 AT3G29185_circ_g.2 AT3G29185_circ_g.3 AT3G29185_circ_g.4 AT3G29185_circ_g.6 AT3G29185_circ_g.7 AT3G29185_circ_g.8 AT3G29185_circ_g.9 AT3G29185_circ_g.10
Support reads	2/9
Tissues	leaf/aerial, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G29185.2:1 AT3G29185.1:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCTTTCT
GCATCGTTctggactacttgacctttgtcattcatttcaagaacagcaacagtatcggcttctg
atagtgttgctccata

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 126
Transcript exons: 11155468-11155523,11155542-11155611
CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGCACCGCTT
CGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCTTTCTGCATCGTT
Transcript length: 144
Transcript exons: 11155468-11155611
CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT
GCTCCATAGACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTA
TCCTTTCTGCATCGTT
Transcript length: 140
Transcript exons: 11155468-11155611
CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT
GCTCCATAGACACCCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCT
TTCTGCATCGTT
						
						Genomic sequence
						
							CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT
GCTCCATAGACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTA
TCCTTTCTGCATCGTT

Sequences
Splice junction sequence	GACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCTTTCT GCATCGTTctggactacttgacctttgtcattcatttcaagaacagcaacagtatcggcttctg atagtgttgctccata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 126 Transcript exons: 11155468-11155523,11155542-11155611 CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGCACCGCTT CGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCTTTCTGCATCGTT Transcript length: 144 Transcript exons: 11155468-11155611 CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT GCTCCATAGACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTA TCCTTTCTGCATCGTT Transcript length: 140 Transcript exons: 11155468-11155611 CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT GCTCCATAGACACCCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTATCCT TTCTGCATCGTT
Genomic sequence	CTGGACTACTTGACCTTTGTCATTCATTTCAAGAACAGCAACAGTATCGGCTTCTGATAGTGTT GCTCCATAGACACCGCTTCGTTTTGTTACCGAACGACCTTTCCAAGTACCGAGAAATGGGTTTA TCCTTTCTGCATCGTT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.523727373

Conservation Information
Conserved circRNAs	NA
PMCS	0.523727373

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							11155495-11155470(-)
11155587-11155608(+)
						
						Potential amino acid sequence
						
							MNDKGQVVQNDAERINPFLGTWKGRSVTKRSGVYGATLSEADTVAVLEMNDKGQVVQNDAERIN
PFLGTWKGRSVTKRSGVYGATLSEADTVAVLEMNDKGQVVQNDAERINPFLGTWKGRSVTKRSG
VYGATLSEADTVAVLEMNDKGQVVQ(-)
MGLSFLHRSGLLDLCHSFQEQQQYRLLIVLLHRHRFVLLPNDLSKYREMGLSFLHRSGLLDLCH
SFQEQQQYRLLIVLLHRHRFVLLPNDLSKYREMGLSFLHRSGLLDLCHSFQEQQQYRLLIVLLH
RHRFVLLPNDLSKYREMGLSFLHR(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	11155495-11155470(-) 11155587-11155608(+)
Potential amino acid sequence	MNDKGQVVQNDAERINPFLGTWKGRSVTKRSGVYGATLSEADTVAVLEMNDKGQVVQNDAERIN PFLGTWKGRSVTKRSGVYGATLSEADTVAVLEMNDKGQVVQNDAERINPFLGTWKGRSVTKRSG VYGATLSEADTVAVLEMNDKGQVVQ(-) MGLSFLHRSGLLDLCHSFQEQQQYRLLIVLLHRHRFVLLPNDLSKYREMGLSFLHRSGLLDLCH SFQEQQQYRLLIVLLHRHRFVLLPNDLSKYREMGLSFLHRSGLLDLCHSFQEQQQYRLLIVLLH RHRFVLLPNDLSKYREMGLSFLHR(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT3G29185_circ_g.5

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