Plant Circular RNA Database

Detail information of Os03g0119500_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0119500_circ_g.4
					
						ID in PlantcircBase
						osa_circ_017626
					
						Alias
						Os_ciR8443
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 1060630-1062976  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os03g0119500						
					
						Parent gene annotation
						
							Callose synthase, Ovary expansion by affecting vascular cell pat
terning (Os03t0119500-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0119500-01:7

General Information
CircRNA Name	Os03g0119500_circ_g.4
ID in PlantcircBase	osa_circ_017626
Alias	Os_ciR8443
Organism	Oryza sativa
Position	chr3: 1060630-1062976 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os03g0119500
Parent gene annotation	Callose synthase, Ovary expansion by affecting vascular cell pat terning (Os03t0119500-01)
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0119500-01:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TATCCGAGAAAGCATTTCCAAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATTGCCCAAT
GTCATTGCCAAGTTAGTTGCTGTAGCAGGAATTTTGatttaccttttggatatccatgtatttt
acaccgtgatgtctgctatatgtgggtttctccttggtgcacgcgatcgcctgggagaggttta
gcttcttg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 657
Transcript exons: 1060630-1060716,1061097-1061182,1061257-1061281,1062205-
1062299,1062388-1062469,1062584-1062744,1062856-1062976
ATTTACCTTTTGGATATCCATGTATTTTACACCGTGATGTCTGCTATATGTGGGTTTCTCCTTG
GTGCACGCGATCGCCTGGGAGAGATTAGGTCTGTTGAAGCAGTTCACAGATTCTTTGAGAAGTT
CCCTGAAGCATTCATGGATAAACTCCATGTTGCTGTACCGAAAAGGAAGCAGCTGCTATCATCG
AGTCAGCATCCAGAATTAAACAAGTTTGATGCATCTAAATTTGCTCCCTTTTGGAACGAAATTG
TGAGGAATATGAGGGAAGAGGATTATATTAATAACACTGAACTGGATTTACTCTTGATGCCTAA
GAATAATGGATCTCTTCCAATTGTGCAGTGGCCTCTCTTTTTGCTTGCTAGCAAGGTTTTCTTA
GCCAAAGATATTGCAATTGATTGTAAAGACTCACAAGAGGAACTATGGCTAAGGATATCAAAGG
ATGAATACATGCAATATGCTGTTGTAGAGTGCTATCACAGCATTTATTATATTCTGACATCTAT
ACTAGATAAAGAAGGACGTCTCTGGGTGGAGAGGATTTATGTTGGTATCCGAGAAAGCATTTCC
AAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATTGCCCAATGTCATTGCCAAGTTAGTTG
CTGTAGCAGGAATTTTG
						
					

						Genomic sequence
						
							ATTTACCTTTTGGATATCCATGTATTTTACACCGTGATGTCTGCTATATGTGGGTTTCTCCTTG
GTGCACGCGATCGCCTGGGAGAGGTTTAGCTTCTTGACTTTTAACTTTTGAGCTATTGCAATGT
GATTCTCTCTTGTGTGTAAAATTGTTATTGTTTATGCGAGGAAATCTTTGCTTGTTTACCAAAT
CCCAAATTCTCCAGCATATTGAAATGTTCAGGATGTTTCTACCTATGAAAATGCATTATTTGTG
GAAAGGCACTTGAAAATAATTTTGTACTATTAACATGCACATATGAAAGTAATTGTACCAATTT
TGTTAAGCCCATTTATGTAAAATGCATGTTTTTTTCTAGACCATTTTCCTTGGCTCTTCATCTG
TGTATAGCTAGTACTTCGATTGTTATTTTGCTTTTTTTTTTTTTGTCAATTTTTCTTATCTGGT
GGTTGTATGCCATTTTCAGATTAGGTCTGTTGAAGCAGTTCACAGATTCTTTGAGAAGTTCCCT
GAAGCATTCATGGATAAACTCCATGTTGCTGTACCGAAAAGGTCTTTGATGTTTTTATTGTTTT
TGATTTATACTTGAGTGGTATACACTAAAGTGGTTCTAATCTGTTATACAGGAAGCAGCTGCTA
TCATCGAGTCAGGTACTATTATCTTGTTACCTGTATTTTGCTTGTTTACTTATTCTATCTTTTC
TGTTGGTGGAGCTGTGGGACATTATTTGTTATCTTTCAATAACGCTCTTAAGTTGCTTTATTTA
TATGATATTGTCCTTTAGTAAAAAACTTGTACTTTCAAACCAGCTTTGGCTAACTGCCATCCGA
CAATATAAGAAAGTTCGAAAAACGAATTCGGAGGGATTTCTATGATGAAAGCACTTTCGTGATG
AGCATGCCCAATTTTCTTATAGCTCCTAGTATTTGTAAACGGATATGCGGTTAAAGTAATTGTT
CCATTTTGCTTGAATTTCTACATTTGTGGAGTATTTCCCCCCTAGTTTTTTATTTTAAAAAACA
GAACTAGAGTACTACTCCGCTAATTTTATACTAGGTAATAAGAAGTATGGCATTGTGGTTCCAT
TTGTGTATTTCCATTAAAAGATTCACTTAAGTAGATAGGCATTCCTGAATGTTGTGTATTTCCA
CATAACTGTCTTTTTCCTCTGAATGTTGTTTCATGATTACTCTCGTCCTAACCCCCTCGATTAC
CAGTCATCTGCTGCTTTTGCCTTAAGTCACATCCTCCCTTAATGGCATTTTTTTAGCATTTTAG
TATTTGCAGGTTATCTGATACAGTTTTTTTTTATAAATATTTTGTTTTCGGTTGTCCTGCTTGT
ATGCATGTGCTATGTAGCCATGATTTGTTGAATGAAATATCTTTGTATTGATTTGAGCTCAGAT
CAGTTGAGCTGTTCCTGAGTGTTTTCATCTTGACTGCTATGCGTGTTTAGAGTTTAGACAAGCT
AGAGAGATCTTTTGTTGTTCATAAATTATAGATATAGCATGCTTTCTTCCAGTTCTTTATCTTT
ACTTCCAATAAGTTGACACTGGTATTCACAATTTAACAGCATCCAGAATTAAACAAGTTTGATG
CATCTAAATTTGCTCCCTTTTGGAACGAAATTGTGAGGAATATGAGGGAAGAGGATTATATTAA
TAACACGTAAGTAAAGTTTCTTGGAATACTATGTTACTGTTCTTTCAAACGTCTTAATTAGATA
TGAGTTTTTTCTGATCTTTCATCCTTACAGTGAACTGGATTTACTCTTGATGCCTAAGAATAAT
GGATCTCTTCCAATTGTGCAGTGGCCTCTCTTTTTGCTTGCTAGCAAGGTCAAAAGTCAAAACT
TATTTTAGCTTTTAAAAAGCTCTCCCTGCATGATTTGATGATTTCCGCATCTTATGTTCGTTTT
GTCACTTTGGCGACCACTTCCATTTTCATTGCAGGTTTTCTTAGCCAAAGATATTGCAATTGAT
TGTAAAGACTCACAAGAGGAACTATGGCTAAGGATATCAAAGGATGAATACATGCAATATGCTG
TTGTAGAGTGCTATCACAGCATTTATTATATTCTGACATCTATACTAGATAAAGAAGGACGTCT
CTGGTAAGGGATTTTATTTATGTTCATCCTGTTCCTTACTTCTTTTCATCTAGTTCAACAGATG
TTTTTTTATCCGTGGGAGGGATGTGTTGGTGATGATTTTATTTTTTTTAGGGTGGAGAGGATTT
ATGTTGGTATCCGAGAAAGCATTTCCAAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATT
GCCCAATGTCATTGCCAAGTTAGTTGCTGTAGCAGGAATTTTG
						
					

			
		

Sequences
Splice junction sequence	TATCCGAGAAAGCATTTCCAAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATTGCCCAAT GTCATTGCCAAGTTAGTTGCTGTAGCAGGAATTTTGatttaccttttggatatccatgtatttt acaccgtgatgtctgctatatgtgggtttctccttggtgcacgcgatcgcctgggagaggttta gcttcttg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 657 Transcript exons: 1060630-1060716,1061097-1061182,1061257-1061281,1062205- 1062299,1062388-1062469,1062584-1062744,1062856-1062976 ATTTACCTTTTGGATATCCATGTATTTTACACCGTGATGTCTGCTATATGTGGGTTTCTCCTTG GTGCACGCGATCGCCTGGGAGAGATTAGGTCTGTTGAAGCAGTTCACAGATTCTTTGAGAAGTT CCCTGAAGCATTCATGGATAAACTCCATGTTGCTGTACCGAAAAGGAAGCAGCTGCTATCATCG AGTCAGCATCCAGAATTAAACAAGTTTGATGCATCTAAATTTGCTCCCTTTTGGAACGAAATTG TGAGGAATATGAGGGAAGAGGATTATATTAATAACACTGAACTGGATTTACTCTTGATGCCTAA GAATAATGGATCTCTTCCAATTGTGCAGTGGCCTCTCTTTTTGCTTGCTAGCAAGGTTTTCTTA GCCAAAGATATTGCAATTGATTGTAAAGACTCACAAGAGGAACTATGGCTAAGGATATCAAAGG ATGAATACATGCAATATGCTGTTGTAGAGTGCTATCACAGCATTTATTATATTCTGACATCTAT ACTAGATAAAGAAGGACGTCTCTGGGTGGAGAGGATTTATGTTGGTATCCGAGAAAGCATTTCC AAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATTGCCCAATGTCATTGCCAAGTTAGTTG CTGTAGCAGGAATTTTG
Genomic sequence	ATTTACCTTTTGGATATCCATGTATTTTACACCGTGATGTCTGCTATATGTGGGTTTCTCCTTG GTGCACGCGATCGCCTGGGAGAGGTTTAGCTTCTTGACTTTTAACTTTTGAGCTATTGCAATGT GATTCTCTCTTGTGTGTAAAATTGTTATTGTTTATGCGAGGAAATCTTTGCTTGTTTACCAAAT CCCAAATTCTCCAGCATATTGAAATGTTCAGGATGTTTCTACCTATGAAAATGCATTATTTGTG GAAAGGCACTTGAAAATAATTTTGTACTATTAACATGCACATATGAAAGTAATTGTACCAATTT TGTTAAGCCCATTTATGTAAAATGCATGTTTTTTTCTAGACCATTTTCCTTGGCTCTTCATCTG TGTATAGCTAGTACTTCGATTGTTATTTTGCTTTTTTTTTTTTTGTCAATTTTTCTTATCTGGT GGTTGTATGCCATTTTCAGATTAGGTCTGTTGAAGCAGTTCACAGATTCTTTGAGAAGTTCCCT GAAGCATTCATGGATAAACTCCATGTTGCTGTACCGAAAAGGTCTTTGATGTTTTTATTGTTTT TGATTTATACTTGAGTGGTATACACTAAAGTGGTTCTAATCTGTTATACAGGAAGCAGCTGCTA TCATCGAGTCAGGTACTATTATCTTGTTACCTGTATTTTGCTTGTTTACTTATTCTATCTTTTC TGTTGGTGGAGCTGTGGGACATTATTTGTTATCTTTCAATAACGCTCTTAAGTTGCTTTATTTA TATGATATTGTCCTTTAGTAAAAAACTTGTACTTTCAAACCAGCTTTGGCTAACTGCCATCCGA CAATATAAGAAAGTTCGAAAAACGAATTCGGAGGGATTTCTATGATGAAAGCACTTTCGTGATG AGCATGCCCAATTTTCTTATAGCTCCTAGTATTTGTAAACGGATATGCGGTTAAAGTAATTGTT CCATTTTGCTTGAATTTCTACATTTGTGGAGTATTTCCCCCCTAGTTTTTTATTTTAAAAAACA GAACTAGAGTACTACTCCGCTAATTTTATACTAGGTAATAAGAAGTATGGCATTGTGGTTCCAT TTGTGTATTTCCATTAAAAGATTCACTTAAGTAGATAGGCATTCCTGAATGTTGTGTATTTCCA CATAACTGTCTTTTTCCTCTGAATGTTGTTTCATGATTACTCTCGTCCTAACCCCCTCGATTAC CAGTCATCTGCTGCTTTTGCCTTAAGTCACATCCTCCCTTAATGGCATTTTTTTAGCATTTTAG TATTTGCAGGTTATCTGATACAGTTTTTTTTTATAAATATTTTGTTTTCGGTTGTCCTGCTTGT ATGCATGTGCTATGTAGCCATGATTTGTTGAATGAAATATCTTTGTATTGATTTGAGCTCAGAT CAGTTGAGCTGTTCCTGAGTGTTTTCATCTTGACTGCTATGCGTGTTTAGAGTTTAGACAAGCT AGAGAGATCTTTTGTTGTTCATAAATTATAGATATAGCATGCTTTCTTCCAGTTCTTTATCTTT ACTTCCAATAAGTTGACACTGGTATTCACAATTTAACAGCATCCAGAATTAAACAAGTTTGATG CATCTAAATTTGCTCCCTTTTGGAACGAAATTGTGAGGAATATGAGGGAAGAGGATTATATTAA TAACACGTAAGTAAAGTTTCTTGGAATACTATGTTACTGTTCTTTCAAACGTCTTAATTAGATA TGAGTTTTTTCTGATCTTTCATCCTTACAGTGAACTGGATTTACTCTTGATGCCTAAGAATAAT GGATCTCTTCCAATTGTGCAGTGGCCTCTCTTTTTGCTTGCTAGCAAGGTCAAAAGTCAAAACT TATTTTAGCTTTTAAAAAGCTCTCCCTGCATGATTTGATGATTTCCGCATCTTATGTTCGTTTT GTCACTTTGGCGACCACTTCCATTTTCATTGCAGGTTTTCTTAGCCAAAGATATTGCAATTGAT TGTAAAGACTCACAAGAGGAACTATGGCTAAGGATATCAAAGGATGAATACATGCAATATGCTG TTGTAGAGTGCTATCACAGCATTTATTATATTCTGACATCTATACTAGATAAAGAAGGACGTCT CTGGTAAGGGATTTTATTTATGTTCATCCTGTTCCTTACTTCTTTTCATCTAGTTCAACAGATG TTTTTTTATCCGTGGGAGGGATGTGTTGGTGATGATTTTATTTTTTTTAGGGTGGAGAGGATTT ATGTTGGTATCCGAGAAAGCATTTCCAAGAGGAACATTCAGAGTGATCTTCATTTCAGCAGATT GCCCAATGTCATTGCCAAGTTAGTTGCTGTAGCAGGAATTTTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.316955696

Conservation Information
Conserved circRNAs	NA
PMCS	0.316955696

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1060667-1062973(+)
1061167-1062955(-)
						
						Potential amino acid sequence
						
							MSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRKQLLSSSQHPELNKFDAS
KFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLASKVFLAKDIAIDCKDSQ
EELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGIRESISKRNIQSDLHFSR
LPNVIAKLVAVAGILIYLLDIHVFYTVMSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMD
KLHVAVPKRKQLLSSSQHPELNKFDASKFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLP
IVQWPLFLLASKVFLAKDIAIDCKDSQEELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGR
LWVERIYVGIRESISKRNIQSDLHFSRLPNVIAKLVAVAGILIYLLDIHVFYTVMSAICGFLLG
ARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRKQLLSSSQHPELNKFDASKFAPFWNEIV
RNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLASKVFLAKDIAIDCKDSQEELWLRISKD
EYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGIRESISKRNIQSDLHFSRLPNVIAKLVA
VAGILIYLLDIHVFYTVMSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRK
QLLSSSQHPELNKFDASKFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLA
SKVFLAKDIAIDCKDSQEELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGI
RESISKRNIQSDLHFSRLPNVIAKLVAVAGIL(+)
MEFIHECFRELLKESVNCFNRPNLSQAIACTKEKPTYSRHHGVKYMDIQKVNQNSCYSN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1060667-1062973(+) 1061167-1062955(-)
Potential amino acid sequence	MSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRKQLLSSSQHPELNKFDAS KFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLASKVFLAKDIAIDCKDSQ EELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGIRESISKRNIQSDLHFSR LPNVIAKLVAVAGILIYLLDIHVFYTVMSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMD KLHVAVPKRKQLLSSSQHPELNKFDASKFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLP IVQWPLFLLASKVFLAKDIAIDCKDSQEELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGR LWVERIYVGIRESISKRNIQSDLHFSRLPNVIAKLVAVAGILIYLLDIHVFYTVMSAICGFLLG ARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRKQLLSSSQHPELNKFDASKFAPFWNEIV RNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLASKVFLAKDIAIDCKDSQEELWLRISKD EYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGIRESISKRNIQSDLHFSRLPNVIAKLVA VAGILIYLLDIHVFYTVMSAICGFLLGARDRLGEIRSVEAVHRFFEKFPEAFMDKLHVAVPKRK QLLSSSQHPELNKFDASKFAPFWNEIVRNMREEDYINNTELDLLLMPKNNGSLPIVQWPLFLLA SKVFLAKDIAIDCKDSQEELWLRISKDEYMQYAVVECYHSIYYILTSILDKEGRLWVERIYVGI RESISKRNIQSDLHFSRLPNVIAKLVAVAGIL(+) MEFIHECFRELLKESVNCFNRPNLSQAIACTKEKPTYSRHHGVKYMDIQKVNQNSCYSN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os03g0119500_circ_g.4

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