Plant Circular RNA Database

Detail information of Zm00001d004091_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d004091_circ_g.2
					
						ID in PlantcircBase
						zma_circ_007211
					
						Alias
						Zm02circ00064, zma_circ_0000776, GRMZM2G065839_C2
					
						Organism
						
							Zea mays						
					
						Position
						
							chr2: 81995014-81995861  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d004091						
					
						Parent gene annotation
						
							Metal tolerance protein C4
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d004091_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d004091_T004:3
Zm00001d004091_T003:4
Zm00001d004091_T001:4
Zm00001d004091_T005:4
Zm00001d004091_T002:4

General Information
CircRNA Name	Zm00001d004091_circ_g.2
ID in PlantcircBase	zma_circ_007211
Alias	Zm02circ00064, zma_circ_0000776, GRMZM2G065839_C2
Organism	Zea mays
Position	chr2: 81995014-81995861 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d004091
Parent gene annotation	Metal tolerance protein C4
Parent gene strand	-
Alternative splicing	Zm00001d004091_circ_g.1
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d004091_T004:3 Zm00001d004091_T003:4 Zm00001d004091_T001:4 Zm00001d004091_T005:4 Zm00001d004091_T002:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATTTTGAACTCCATGCACAATTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATG
AGAGACCATACAAATCTTTCCTTAGAATAACCATAGcatccccagtaaattgccaacaatgatt
gagccaattggatcatacatagcatttcctgtcatttgaacagctaccaaagatgctgcagcaa
tagcaagg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 412
Transcript exons: 81995014-81995133,81995343-81995461,81995565-81995622,81
995747-81995861
CATCCCCAGTAAATTGCCAACAATGATTGAGCCAATTGGATCATACATAGCATTTCCTGTCATT
TGAACAGCTACCAAAGATGCTGCAGCAATAGCAAGGCCTGTAACAGCAGCACCATCTTCAGTCA
TAACAGCAACGGAAGTTGGATCATGACCACGCCAGATATAGTCCCAGATACTCATTCCCTCTGC
TTCTGCACCTTTCCTAACAGCCTTTATAGCAACAAGCAGTGAAGCACCTTCAATTAGGAAGGAC
CCACCAATCACTAATGCAGCCCAGTGAATATTCTCTGGAGGATGAGAATTCCATAAATTTTGAA
CTCCATGCACAATTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATGAGAGACCA
TACAAATCTTTCCTTAGAATAACCATAG
						
						Genomic sequence
						
							CATCCCCAGTAAATTGCCAACAATGATTGAGCCAATTGGATCATACATAGCATTTCCTGTCATT
TGAACAGCTACCAAAGATGCTGCAGCAATAGCAAGGCCTGTAACAGCAGCACCATCCTGTAGAC
AAATATCCAATGTGAAAAATGAGAATGAAATCAATACAGAAAAGTACTAGGTGATGAAAGGTAA
ATGAACAAGAAATCAATTAAGAAAACTATGAGATAATAGTTTCCATTAAAGTCAACCATTACAA
ATGGCAGAAACGAAAATGAGTATGATGATGTGATTGAACTACATAAAAACTGTTAGGTAGTCAT
ATTACTAACTTCAGTCATAACAGCAACGGAAGTTGGATCATGACCACGCCAGATATAGTCCCAG
ATACTCATTCCCTCTGCTTCTGCACCTTTCCTAACAGCCTTTATAGCAACAAGCAGTGAAGCAC
CTAACAGACAACAGGATTTCACAAGTAAATGTAAATTATAGCTAGAATAGACACCCCAACAAAA
AGAGAGGTATTGAGAGAGCTAGCAGCAAGCGAGAATCACCTTCAATTAGGAAGGACCCACCAAT
CACTAATGCAGCCCAGTGAATATTCTCTGGAGGCTGTCAAACATAAGTATGGATATGAACATTA
TCGTACTTGGATGGCGATTGATGACATTTGAAAATAAAATGTAATTCTGTGCATAAAAGTTATT
ATGACTCAAATGACACTTTGGTGACGTACATGAGAATTCCATAAATTTTGAACTCCATGCACAA
TTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATGAGAGACCATACAAATCTTTC
CTTAGAATAACCATAG

Sequences
Splice junction sequence	ATTTTGAACTCCATGCACAATTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATG AGAGACCATACAAATCTTTCCTTAGAATAACCATAGcatccccagtaaattgccaacaatgatt gagccaattggatcatacatagcatttcctgtcatttgaacagctaccaaagatgctgcagcaa tagcaagg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 412 Transcript exons: 81995014-81995133,81995343-81995461,81995565-81995622,81 995747-81995861 CATCCCCAGTAAATTGCCAACAATGATTGAGCCAATTGGATCATACATAGCATTTCCTGTCATT TGAACAGCTACCAAAGATGCTGCAGCAATAGCAAGGCCTGTAACAGCAGCACCATCTTCAGTCA TAACAGCAACGGAAGTTGGATCATGACCACGCCAGATATAGTCCCAGATACTCATTCCCTCTGC TTCTGCACCTTTCCTAACAGCCTTTATAGCAACAAGCAGTGAAGCACCTTCAATTAGGAAGGAC CCACCAATCACTAATGCAGCCCAGTGAATATTCTCTGGAGGATGAGAATTCCATAAATTTTGAA CTCCATGCACAATTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATGAGAGACCA TACAAATCTTTCCTTAGAATAACCATAG
Genomic sequence	CATCCCCAGTAAATTGCCAACAATGATTGAGCCAATTGGATCATACATAGCATTTCCTGTCATT TGAACAGCTACCAAAGATGCTGCAGCAATAGCAAGGCCTGTAACAGCAGCACCATCCTGTAGAC AAATATCCAATGTGAAAAATGAGAATGAAATCAATACAGAAAAGTACTAGGTGATGAAAGGTAA ATGAACAAGAAATCAATTAAGAAAACTATGAGATAATAGTTTCCATTAAAGTCAACCATTACAA ATGGCAGAAACGAAAATGAGTATGATGATGTGATTGAACTACATAAAAACTGTTAGGTAGTCAT ATTACTAACTTCAGTCATAACAGCAACGGAAGTTGGATCATGACCACGCCAGATATAGTCCCAG ATACTCATTCCCTCTGCTTCTGCACCTTTCCTAACAGCCTTTATAGCAACAAGCAGTGAAGCAC CTAACAGACAACAGGATTTCACAAGTAAATGTAAATTATAGCTAGAATAGACACCCCAACAAAA AGAGAGGTATTGAGAGAGCTAGCAGCAAGCGAGAATCACCTTCAATTAGGAAGGACCCACCAAT CACTAATGCAGCCCAGTGAATATTCTCTGGAGGCTGTCAAACATAAGTATGGATATGAACATTA TCGTACTTGGATGGCGATTGATGACATTTGAAAATAAAATGTAATTCTGTGCATAAAAGTTATT ATGACTCAAATGACACTTTGGTGACGTACATGAGAATTCCATAAATTTTGAACTCCATGCACAA TTGTAGCACCGGAACCCAAGCAAAATATTCCAACTGCAGATATGAGAGACCATACAAATCTTTC CTTAGAATAACCATAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.286994797

Conservation Information
Conserved circRNAs	NA
PMCS	0.286994797

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							81995775-81995036(+)
81995407-81995849(-)
81995068-81995849(-)
						
						Potential amino acid sequence
						
							MHNCSTGTQAKYSNCRYERPYKSFLRITIASPVNCQQ*(+)
MSIWDYIWRGHDPTSVAVMTEDGAAVTGLAIAAASLVAVQMTGNAMYDPIGSIIVGNLLGMLWL
F*(-)
MLCMIQLAQSLLAIYWGCYGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWNSHPPENIHWA
ALVIGGSFLIEGASLLVAIKAVRKGAEAEGMSIWDYIWRGHDPTSVAVMTEDGAAVTGLAIAAA
SLVAVQMTGNAMYDPIGSIIVGNLLGMLWLF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	81995775-81995036(+) 81995407-81995849(-) 81995068-81995849(-)
Potential amino acid sequence	MHNCSTGTQAKYSNCRYERPYKSFLRITIASPVNCQQ(+) MSIWDYIWRGHDPTSVAVMTEDGAAVTGLAIAAASLVAVQMTGNAMYDPIGSIIVGNLLGMLWL F(-) MLCMIQLAQSLLAIYWGCYGYSKERFVWSLISAVGIFCLGSGATIVHGVQNLWNSHPPENIHWA ALVIGGSFLIEGASLLVAIKAVRKGAEAEGMSIWDYIWRGHDPTSVAVMTEDGAAVTGLAIAAA SLVAVQMTGNAMYDPIGSIIVGNLLGMLWLF*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d004091_circ_g.2

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