Plant Circular RNA Database

Detail information of AT2G27210_circ_g.8

			
						 General Information
					
						CircRNA Name
						AT2G27210_circ_g.8
					
						ID in PlantcircBase
						ath_circ_015173
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 11635010-11635368  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, CIRI-full
					
						Parent gene
						
							AT2G27210						
					
						Parent gene annotation
						
							Serine/threonine-protein phosphatase BSL3
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G27210_circ_g.7 						
					
						Support reads
						9/NA
					
						Tissues
						whole_plant/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G27210.2:2
AT2G27210.1:2

General Information
CircRNA Name	AT2G27210_circ_g.8
ID in PlantcircBase	ath_circ_015173
Alias	NA
Organism	Arabidpsis thaliana
Position	chr2: 11635010-11635368 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, CIRI-full
Parent gene	AT2G27210
Parent gene annotation	Serine/threonine-protein phosphatase BSL3
Parent gene strand	+
Alternative splicing	AT2G27210_circ_g.7
Support reads	9/NA
Tissues	whole_plant/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G27210.2:2 AT2G27210.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTAAGG
CCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGGggtgaacgtgatgggatctgggtgtggc
atcgaataaaccgtttattcaattggctacctctggctgcactgattgagaagaaaatcatctg
tatgcatg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 230
Transcript exons: 11635010-11635075,11635113-11635211,11635304-11635368
GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG
CTGAATTGGTCGGTCGATAAATCATGTGGAGCAGATAGAAAATATCCAACGTCCAATTACGATG
GAAGCAGGCTCAATTGTACTTATGGATTTATTATGGTCTGAAAATGACAGTGTCGAAGGTTTAA
GGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG
Transcript length: 276
Transcript exons: 11635010-11635209,11635293-11635368
GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG
CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT
GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT
TTATTATGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTAAGGCCCAATGCTAGAGGCC
CTGGGTTGGTTACTTTTGGG
Transcript length: 359
Transcript exons: 11635010-11635368
GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG
CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT
GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT
TTATTATGGTAAGTTCATGTCTAATGCTGTATCATTCTATGTTGTTTCCGTGATCCTTGTCTCA
CTAGTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTA
AGGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG
						
					

						Genomic sequence
						
							GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG
CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT
GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT
TTATTATGGTAAGTTCATGTCTAATGCTGTATCATTCTATGTTGTTTCCGTGATCCTTGTCTCA
CTAGTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTA
AGGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG
						
					

			
		

Sequences
Splice junction sequence	GTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTAAGG CCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGGggtgaacgtgatgggatctgggtgtggc atcgaataaaccgtttattcaattggctacctctggctgcactgattgagaagaaaatcatctg tatgcatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 230 Transcript exons: 11635010-11635075,11635113-11635211,11635304-11635368 GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG CTGAATTGGTCGGTCGATAAATCATGTGGAGCAGATAGAAAATATCCAACGTCCAATTACGATG GAAGCAGGCTCAATTGTACTTATGGATTTATTATGGTCTGAAAATGACAGTGTCGAAGGTTTAA GGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG Transcript length: 276 Transcript exons: 11635010-11635209,11635293-11635368 GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT TTATTATGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTAAGGCCCAATGCTAGAGGCC CTGGGTTGGTTACTTTTGGG Transcript length: 359 Transcript exons: 11635010-11635368 GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT TTATTATGGTAAGTTCATGTCTAATGCTGTATCATTCTATGTTGTTTCCGTGATCCTTGTCTCA CTAGTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTA AGGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG
Genomic sequence	GGTGAACGTGATGGGATCTGGGTGTGGCATCGAATAAACCGTTTATTCAATTGGCTACCTCTGG CTGCACTGATTGAGAAGAAAATCATCTGTATGCATGGGGGAATTGGTCGGTCGATAAATCATGT GGAGCAGATAGAAAATATCCAACGTCCAATTACGATGGAAGCAGGCTCAATTGTACTTATGGAT TTATTATGGTAAGTTCATGTCTAATGCTGTATCATTCTATGTTGTTTCCGTGATCCTTGTCTCA CTAGTATGTCATCTTGTAATCTGTTAGGTCGGATCCAACTGAAAATGACAGTGTCGAAGGTTTA AGGCCCAATGCTAGAGGCCCTGGGTTGGTTACTTTTGGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.64172232

Conservation Information
Conserved circRNAs	NA
PMCS	0.64172232

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							11635103-11635365(+)
						
						Potential amino acid sequence
						
							MHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDSVEGLRPNARGPGLVTFGGER
DGIWVWHRINRLFNWLPLAALIEKKIICMHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLW
SDPTENDSVEGLRPNARGPGLVTFGGERDGIWVWHRINRLFNWLPLAALIEKKIICMHGGIGRS
INHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDSVEGLRPNARGPGLVTFGGERDGIWVWHR
INRLFNWLPLAALIEKKIICMHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDS
VEGLRPNARGPGLVTFG(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	11635103-11635365(+)
Potential amino acid sequence	MHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDSVEGLRPNARGPGLVTFGGER DGIWVWHRINRLFNWLPLAALIEKKIICMHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLW SDPTENDSVEGLRPNARGPGLVTFGGERDGIWVWHRINRLFNWLPLAALIEKKIICMHGGIGRS INHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDSVEGLRPNARGPGLVTFGGERDGIWVWHR INRLFNWLPLAALIEKKIICMHGGIGRSINHVEQIENIQRPITMEAGSIVLMDLLWSDPTENDS VEGLRPNARGPGLVTFG(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Liu et al., 2017

circRNA

Detail information of AT2G27210_circ_g.8

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