Plant Circular RNA Database

Detail information of orai.010G076500_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.010G076500_circ_g.1
					
						ID in PlantcircBase
						gra_circ_001099
					
						Alias
						Chr10:10997785|10998277
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr10: 10997785-10998277  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.010G076500						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						4/14
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.010G076500.1:2

General Information
CircRNA Name	orai.010G076500_circ_g.1
ID in PlantcircBase	gra_circ_001099
Alias	Chr10:10997785\|10998277
Organism	Gossypium raimondii
Position	chrChr10: 10997785-10998277 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.010G076500
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	4/14
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.010G076500.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCTCTGGATAACCAGTTCCATTATCTTTCAG
ATATGGATTCAACTCTCTAGGATGCATCGTCTTTATcctttcagaatttctttctgactttctt
tccctttgattgtctgcaacattttgtttctcattatctgggccttgctttctatttcttatgg
catgcaga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 384
Transcript exons: 10997785-10997964,10998074-10998277
CCTTTCAGAATTTCTTTCTGACTTTCTTTCCCTTTGATTGTCTGCAACATTTTGTTTCTCATTA
TCTGGGCCTTGCTTTCTATTTCTTATGGCATGCAGATGGGCTCGAGGTGCAGCAGCTCTACGAG
ATGCTCCATATGCAGCCAGTATATCAAGAGAACCCCAACGTTCTAGAACAACCTCCTCAATACT
TCGTCCTTCTCGAGAAGCTTGCTCTTCTGCACGCTTCAAAGCTTTAAGTCTCCAACTAGCACCT
CCATCCCCTACAAACGAAGAAGATAAAAGCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCT
CTGGATAACCAGTTCCATTATCTTTCAGATATGGATTCAACTCTCTAGGATGCATCGTCTTTAT
						
						Genomic sequence
						
							CCTTTCAGAATTTCTTTCTGACTTTCTTTCCCTTTGATTGTCTGCAACATTTTGTTTCTCATTA
TCTGGGCCTTGCTTTCTATTTCTTATGGCATGCAGATGGGCTCGAGGTGCAGCAGCTCTACGAG
ATGCTCCATATGCAGCCAGTATATCAAGAGAACCCCAACGTTCTAGAACAACCTGTATGGTTTC
AACAGTAAAGGCTAATGGAATCACACTTTAAACTGTTAAAAAAAAAAAAAAAAAGCAACATGGC
ACCCTTCCTGATATACAATTTTCCAAAAATTACCTCCTCAATACTTCGTCCTTCTCGAGAAGCT
TGCTCTTCTGCACGCTTCAAAGCTTTAAGTCTCCAACTAGCACCTCCATCCCCTACAAACGAAG
AAGATAAAAGCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCTCTGGATAACCAGTTCCATT
ATCTTTCAGATATGGATTCAACTCTCTAGGATGCATCGTCTTTAT

Sequences
Splice junction sequence	GCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCTCTGGATAACCAGTTCCATTATCTTTCAG ATATGGATTCAACTCTCTAGGATGCATCGTCTTTATcctttcagaatttctttctgactttctt tccctttgattgtctgcaacattttgtttctcattatctgggccttgctttctatttcttatgg catgcaga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 384 Transcript exons: 10997785-10997964,10998074-10998277 CCTTTCAGAATTTCTTTCTGACTTTCTTTCCCTTTGATTGTCTGCAACATTTTGTTTCTCATTA TCTGGGCCTTGCTTTCTATTTCTTATGGCATGCAGATGGGCTCGAGGTGCAGCAGCTCTACGAG ATGCTCCATATGCAGCCAGTATATCAAGAGAACCCCAACGTTCTAGAACAACCTCCTCAATACT TCGTCCTTCTCGAGAAGCTTGCTCTTCTGCACGCTTCAAAGCTTTAAGTCTCCAACTAGCACCT CCATCCCCTACAAACGAAGAAGATAAAAGCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCT CTGGATAACCAGTTCCATTATCTTTCAGATATGGATTCAACTCTCTAGGATGCATCGTCTTTAT
Genomic sequence	CCTTTCAGAATTTCTTTCTGACTTTCTTTCCCTTTGATTGTCTGCAACATTTTGTTTCTCATTA TCTGGGCCTTGCTTTCTATTTCTTATGGCATGCAGATGGGCTCGAGGTGCAGCAGCTCTACGAG ATGCTCCATATGCAGCCAGTATATCAAGAGAACCCCAACGTTCTAGAACAACCTGTATGGTTTC AACAGTAAAGGCTAATGGAATCACACTTTAAACTGTTAAAAAAAAAAAAAAAAAGCAACATGGC ACCCTTCCTGATATACAATTTTCCAAAAATTACCTCCTCAATACTTCGTCCTTCTCGAGAAGCT TGCTCTTCTGCACGCTTCAAAGCTTTAAGTCTCCAACTAGCACCTCCATCCCCTACAAACGAAG AAGATAAAAGCCTGTCTACACCAGCTCTCTTTTCTTCTGCTTCCTCTGGATAACCAGTTCCATT ATCTTTCAGATATGGATTCAACTCTCTAGGATGCATCGTCTTTAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							ghi_circ_000300* gar_circ_000877* ghi_circ_000085* 						
					
						PMCS
						1.101562847

Conservation Information
Conserved circRNAs	ghi_circ_000300* gar_circ_000877* ghi_circ_000085*
PMCS	1.101562847

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10998269-10997787(-)
10997847-10998276(-)
						
						Potential amino acid sequence
						
							MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE
EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT
MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE
EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT
MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE
EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT
MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE
EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSER(-)
MRNKMLQTIKGKESQKEILKG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	10998269-10997787(-) 10997847-10998276(-)
Potential amino acid sequence	MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSERIKT MHPRELNPYLKDNGTGYPEEAEEKRAGVDRLLSSSFVGDGGASWRLKALKRAEEQASREGRSIE EVVLERWGSLDILAAYGASRRAAAPRAHLHAIRNRKQGPDNEKQNVADNQRERKSERNSER(-) MRNKMLQTIKGKESQKEILKG*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.010G076500_circ_g.1

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