Plant Circular RNA Database

Detail information of Os05g0519400_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os05g0519400_circ_g.5
					
						ID in PlantcircBase
						osa_circ_028543
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 25783526-25784946  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0519400						
					
						Parent gene annotation
						
							Homologue of NSF (N-ethylmaleimide sensitive factor), ATPase, Re
gulation of vacuolar morphology in endosperm cell (Os05t0519400-
01);Similar to predicted protein. (Os05t0519400-02);Similar to p
redicted protein. (Os05t0519400-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0519400_circ_g.3 Os05g0519400_circ_g.4 Os05g0519400_circ_g.6 Os05g0519400_circ_g.7 Os05g0519400_circ_g.8 Os05g0519400_circ_g.9 Os05g0519400_circ_g.10 Os05g0519400_circ_g.11 Os05g0519400_circ_g.12 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0519400-02:4
Os05t0519400-03:4
Os05t0519400-01:4

General Information
CircRNA Name	Os05g0519400_circ_g.5
ID in PlantcircBase	osa_circ_028543
Alias	NA
Organism	Oryza sativa
Position	chr5: 25783526-25784946 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0519400
Parent gene annotation	Homologue of NSF (N-ethylmaleimide sensitive factor), ATPase, Re gulation of vacuolar morphology in endosperm cell (Os05t0519400- 01);Similar to predicted protein. (Os05t0519400-02);Similar to p redicted protein. (Os05t0519400-03)
Parent gene strand	-
Alternative splicing	Os05g0519400_circ_g.3 Os05g0519400_circ_g.4 Os05g0519400_circ_g.6 Os05g0519400_circ_g.7 Os05g0519400_circ_g.8 Os05g0519400_circ_g.9 Os05g0519400_circ_g.10 Os05g0519400_circ_g.11 Os05g0519400_circ_g.12
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0519400-02:4 Os05t0519400-03:4 Os05t0519400-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGAGCTTTCTTTCATCTTATTCGTATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGC
AGGTTTATCTCAATATGAACTTCCAATCGTCCAGGCaattttgacgtaagcaaaatcactgtca
atgccaacagtagcagccaaagccgatttaccactgacaagcaaaggaaatgttaattgaattg
aggctctg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 538
Transcript exons: 25783526-25783586,25783672-25783803,25784255-25784465,25
784813-25784946
AATTTTGACGTAAGCAAAATCACTGTCAATGCCAACAGTAGCAGCCAAAGCCGATTTACCACTT
CCAGCAGGACCTTCCAAGAGGCATGTCACAAGTGGACTACCTTTGCTAACTTTAACTTGCTCAA
CAAGAAGCATACCTCTCTCATACAAGTGCCTATGTGCCTTGCCACAATCAACCATACCCCTTAG
CCTGCATCTTTCAAGGTCATCAGTGGAAGCACCAAATGCAGGGGTAATTTCATGAAGTGCATTC
ACAAAATCATCCATAGTAACCTTAATGCTTTCTTCATCCAAAGGCTTGGTAAGGTCATCCATGC
TTATCTGCCTGTTCAAAGCATATGAGACTGCACTTTTGACGACACCTTCCAGCTCTGCTCCACT
GTAGTTCTTTGTCCGTGCAGCTAGCTCCTGAAGATTAACATTCGGGGAAAGGAAAGAGCTTTCT
TTCATCTTATTCGTATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGCAGGTTTATCT
CAATATGAACTTCCAATCGTCCAGGC
						
					

						Genomic sequence
						
							AATTTTGACGTAAGCAAAATCACTGTCAATGCCAACAGTAGCAGCCAAAGCCGATTTACCACTG
ACAAGCAAAGGAAATGTTAATTGAATTGAGGCTCTGGTAATGTTTTTAAAGATGAACATTCTGT
TTTAGTTAGTTACAATACCTTCCAGCAGGACCTTCCAAGAGGCATGTCACAAGTGGACTACCTT
TGCTAACTTTAACTTGCTCAACAAGAAGCATACCTCTCTCATACAAGTGCCTATGTGCCTTGCC
ACAATCAACCATACCCCTTAGCCTGTAATTTTTGCACAAAGGTATGGAAAATAGCATTAAACTT
CTACCAAATATTCAGACAAACAGTAAAGAGTAAATAGAACACATGAAATTAATGAATGAAAGGG
CATAATGCATTAGGTCATCCAAAACAATAAATTATGATAAATAACCAAGTGATCTGTTTAGCAA
AATATTAAATTGACATGAGTTATCATTAATAAAAAAGATGTATTAATGCAAAGGAAAAAGCCCT
ATCAACTACTACACAAGAATGACATGCCCATAGCCAACAATGACTATGAATTAGGAATTGCATT
GTGCCGTATCAAACGGCATAAGAATTAGATAGTCCCATGGGACACAGGAAAATAAAAAATATAA
ATTTGAAAGATGAGCCCAGAAACACCCTAAGAAGAAACTGATCATTAAACATTTTGCTAAAGCA
ATGCTCGGGGTATCATAGCACAGACCTGCATCTTTCAAGGTCATCAGTGGAAGCACCAAATGCA
GGGGTAATTTCATGAAGTGCATTCACAAAATCATCCATAGTAACCTTAATGCTTTCTTCATCCA
AAGGCTTGGTAAGGTCATCCATGCTTATCTGCCTGTTCAAAGCATATGAGACTGCACTTTTGAC
GACACCTTCCAGCTCTGCTCCACTGTAGTTCTTTGTCCGTGCAGCTTCATTCAGAAGAGCATGT
CACAACCATCAATCACATTATTTTTGTAGATAATCGATTAAAACCCAGCCTCTACAACCAACAT
GGATGTACACAGCCAAGATCACATGCAGCACATATCTTTTAGAACAAGAAATTCCCACAGGCAC
CTACTCAGTCATACTAAAATGGGTGTTGAAGTAAGAAGCATTGCTAAGACAGCAATTTTTGGAA
ATGGGAAACTAAAACCTTTGAAGCTTGAGTAATAAGACTAGAATGAGCTAATCAGTCCATTTTA
AGAGATCATTTACAACATTTGTTATAATGAACGAATGCCTGAAAACAAATATGAATTATCAAAG
AATATACCTAGCTCCTGAAGATTAACATTCGGGGAAAGGAAAGAGCTTTCTTTCATCTTATTCG
TATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGCAGGTTTATCTCAATATGAACTTC
CAATCGTCCAGGC
						
					

			
		

Sequences
Splice junction sequence	AGAGCTTTCTTTCATCTTATTCGTATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGC AGGTTTATCTCAATATGAACTTCCAATCGTCCAGGCaattttgacgtaagcaaaatcactgtca atgccaacagtagcagccaaagccgatttaccactgacaagcaaaggaaatgttaattgaattg aggctctg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 538 Transcript exons: 25783526-25783586,25783672-25783803,25784255-25784465,25 784813-25784946 AATTTTGACGTAAGCAAAATCACTGTCAATGCCAACAGTAGCAGCCAAAGCCGATTTACCACTT CCAGCAGGACCTTCCAAGAGGCATGTCACAAGTGGACTACCTTTGCTAACTTTAACTTGCTCAA CAAGAAGCATACCTCTCTCATACAAGTGCCTATGTGCCTTGCCACAATCAACCATACCCCTTAG CCTGCATCTTTCAAGGTCATCAGTGGAAGCACCAAATGCAGGGGTAATTTCATGAAGTGCATTC ACAAAATCATCCATAGTAACCTTAATGCTTTCTTCATCCAAAGGCTTGGTAAGGTCATCCATGC TTATCTGCCTGTTCAAAGCATATGAGACTGCACTTTTGACGACACCTTCCAGCTCTGCTCCACT GTAGTTCTTTGTCCGTGCAGCTAGCTCCTGAAGATTAACATTCGGGGAAAGGAAAGAGCTTTCT TTCATCTTATTCGTATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGCAGGTTTATCT CAATATGAACTTCCAATCGTCCAGGC
Genomic sequence	AATTTTGACGTAAGCAAAATCACTGTCAATGCCAACAGTAGCAGCCAAAGCCGATTTACCACTG ACAAGCAAAGGAAATGTTAATTGAATTGAGGCTCTGGTAATGTTTTTAAAGATGAACATTCTGT TTTAGTTAGTTACAATACCTTCCAGCAGGACCTTCCAAGAGGCATGTCACAAGTGGACTACCTT TGCTAACTTTAACTTGCTCAACAAGAAGCATACCTCTCTCATACAAGTGCCTATGTGCCTTGCC ACAATCAACCATACCCCTTAGCCTGTAATTTTTGCACAAAGGTATGGAAAATAGCATTAAACTT CTACCAAATATTCAGACAAACAGTAAAGAGTAAATAGAACACATGAAATTAATGAATGAAAGGG CATAATGCATTAGGTCATCCAAAACAATAAATTATGATAAATAACCAAGTGATCTGTTTAGCAA AATATTAAATTGACATGAGTTATCATTAATAAAAAAGATGTATTAATGCAAAGGAAAAAGCCCT ATCAACTACTACACAAGAATGACATGCCCATAGCCAACAATGACTATGAATTAGGAATTGCATT GTGCCGTATCAAACGGCATAAGAATTAGATAGTCCCATGGGACACAGGAAAATAAAAAATATAA ATTTGAAAGATGAGCCCAGAAACACCCTAAGAAGAAACTGATCATTAAACATTTTGCTAAAGCA ATGCTCGGGGTATCATAGCACAGACCTGCATCTTTCAAGGTCATCAGTGGAAGCACCAAATGCA GGGGTAATTTCATGAAGTGCATTCACAAAATCATCCATAGTAACCTTAATGCTTTCTTCATCCA AAGGCTTGGTAAGGTCATCCATGCTTATCTGCCTGTTCAAAGCATATGAGACTGCACTTTTGAC GACACCTTCCAGCTCTGCTCCACTGTAGTTCTTTGTCCGTGCAGCTTCATTCAGAAGAGCATGT CACAACCATCAATCACATTATTTTTGTAGATAATCGATTAAAACCCAGCCTCTACAACCAACAT GGATGTACACAGCCAAGATCACATGCAGCACATATCTTTTAGAACAAGAAATTCCCACAGGCAC CTACTCAGTCATACTAAAATGGGTGTTGAAGTAAGAAGCATTGCTAAGACAGCAATTTTTGGAA ATGGGAAACTAAAACCTTTGAAGCTTGAGTAATAAGACTAGAATGAGCTAATCAGTCCATTTTA AGAGATCATTTACAACATTTGTTATAATGAACGAATGCCTGAAAACAAATATGAATTATCAAAG AATATACCTAGCTCCTGAAGATTAACATTCGGGGAAAGGAAAGAGCTTTCTTTCATCTTATTCG TATGAATTTGAAGAATCTGCAAACGGCCATTTTCATCAGGCAGGTTTATCTCAATATGAACTTC CAATCGTCCAGGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.267655425

Conservation Information
Conserved circRNAs	NA
PMCS	0.267655425

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							25784872-25783530(+)
25784863-25784922(-)
						
						Potential amino acid sequence
						
							MNLKNLQTAIFIRQVYLNMNFQSSRQF*(+)
MKESSFLSPNVNLQELAARTKNYSGAELEGVVKSAVSYALNRQISMDDLTKPLDEESIKVTMDD
FVNALHEITPAFGASTDDLERCRLRGMVDCGKAHRHLYERGMLLVEQVKVSKGSPLVTCLLEGP
AGSGKSALAATVGIDSDFAYVKIAWTIGSSY*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	25784872-25783530(+) 25784863-25784922(-)
Potential amino acid sequence	MNLKNLQTAIFIRQVYLNMNFQSSRQF(+) MKESSFLSPNVNLQELAARTKNYSGAELEGVVKSAVSYALNRQISMDDLTKPLDEESIKVTMDD FVNALHEITPAFGASTDDLERCRLRGMVDCGKAHRHLYERGMLLVEQVKVSKGSPLVTCLLEGP AGSGKSALAATVGIDSDFAYVKIAWTIGSSY(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0519400_circ_g.5

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