Plant Circular RNA Database

Detail information of Zm00001d047912_circ_g.3

			
						 General Information
					
						CircRNA Name
						Zm00001d047912_circ_g.3
					
						ID in PlantcircBase
						zma_circ_010065
					
						Alias
						Zm09circ00068, GRMZM2G101001_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 145086011-145086320  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d047912						
					
						Parent gene annotation
						
							Translation initiation factor IF-2
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d047912_circ_g.1 Zm00001d047912_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d047912_T003:2
Zm00001d047912_T001:2
Zm00001d047912_T002:2

General Information
CircRNA Name	Zm00001d047912_circ_g.3
ID in PlantcircBase	zma_circ_010065
Alias	Zm09circ00068, GRMZM2G101001_C1
Organism	Zea mays
Position	chr9: 145086011-145086320 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2
Parent gene	Zm00001d047912
Parent gene annotation	Translation initiation factor IF-2
Parent gene strand	-
Alternative splicing	Zm00001d047912_circ_g.1 Zm00001d047912_circ_g.2
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d047912_T003:2 Zm00001d047912_T001:2 Zm00001d047912_T002:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATACCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTTGGATGTCTTGGCATTACT
TCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGGttcttcaggaggatcatgcttctgccct
ggcaaaccatacactttcctctctttcttaaccttctctccctttacaatggccaaggccaact
ttgcctct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 234
Transcript exons: 145086011-145086148,145086225-145086320
TTCTTCAGGAGGATCATGCTTCTGCCCTGGCAAACCATACACTTTCCTCTCTTTCTTAACCTTC
TCTCCCTTTACAATGGCCAAGGCCAACTTTGCCTCTTCTTTGACACCCACTCGTTTCTTCCTTT
TCTTGTCCTCCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTTGGATGTCTTGGC
ATTACTTCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGG
						
						Genomic sequence
						
							TTCTTCAGGAGGATCATGCTTCTGCCCTGGCAAACCATACACTTTCCTCTCTTTCTTAACCTTC
TCTCCCTTTACAATGGCCAAGGCCAACTTTGCCTCTTCTTTGACACCCACTCGTTTCTTCCTTT
TCTTGTCCTCCTTCACCAAGTCACAAGATAATAAGCAAATTTACAACACAACAGGATATCAACC
TAAGTACAAGTAGATTGCATACCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTT
GGATGTCTTGGCATTACTTCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGG

Sequences
Splice junction sequence	ATACCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTTGGATGTCTTGGCATTACT TCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGGttcttcaggaggatcatgcttctgccct ggcaaaccatacactttcctctctttcttaaccttctctccctttacaatggccaaggccaact ttgcctct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 234 Transcript exons: 145086011-145086148,145086225-145086320 TTCTTCAGGAGGATCATGCTTCTGCCCTGGCAAACCATACACTTTCCTCTCTTTCTTAACCTTC TCTCCCTTTACAATGGCCAAGGCCAACTTTGCCTCTTCTTTGACACCCACTCGTTTCTTCCTTT TCTTGTCCTCCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTTGGATGTCTTGGC ATTACTTCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGG
Genomic sequence	TTCTTCAGGAGGATCATGCTTCTGCCCTGGCAAACCATACACTTTCCTCTCTTTCTTAACCTTC TCTCCCTTTACAATGGCCAAGGCCAACTTTGCCTCTTCTTTGACACCCACTCGTTTCTTCCTTT TCTTGTCCTCCTTCACCAAGTCACAAGATAATAAGCAAATTTACAACACAACAGGATATCAACC TAAGTACAAGTAGATTGCATACCTTGAGATCTTCCAAATCTTCATCTTTAATTTTTTTAACCTT GGATGTCTTGGCATTACTTCCAGCAGCACCTGCACTTTTCTTCTGCGCCGTTGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.230541061

Conservation Information
Conserved circRNAs	NA
PMCS	0.230541061

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							145086027-145086317(+)
145086088-145086317(+)
145086270-145086066(+)
145086050-145086084(-)
						
						Potential amino acid sequence
						
							MLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFLPFLVLLEIFQIFIFNFFNLGCLGITSSS
TCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFLPFLVLLEIFQIFIF
NFFNLGCLGITSSSTCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFL
PFLVLLEIFQIFIFNFFNLGCLGITSSSTCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNG
QGQLCLFFDTHSFLPFLVLLEIFQIFIFNFFNLGCLGITSSSTCTFLLRRW(+)
MAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLDVLALLPAAPALFFCAVGSSGGSCFCPGK
PYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLDVLALLPAAPALFFC
AVGSSGGSCFCPGKPYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLD
VLALLPAAPALFFCAVGSSGGSCFCPGKPYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSL
RSSKSSSLIFLTLDVLALLPAAPALFFCAV(+)
MSWHYFQQHLHFSSAPLVLQEDHASALANHTLSSLS*(+)
MVCQGRSMILLKNQRRRRKVQVLLEVMPRHPRLKKLKMKIWKISRRTRKGRNEWVSKKRQSWPW
PL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	145086027-145086317(+) 145086088-145086317(+) 145086270-145086066(+) 145086050-145086084(-)
Potential amino acid sequence	MLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFLPFLVLLEIFQIFIFNFFNLGCLGITSSS TCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFLPFLVLLEIFQIFIF NFFNLGCLGITSSSTCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNGQGQLCLFFDTHSFL PFLVLLEIFQIFIFNFFNLGCLGITSSSTCTFLLRRWFFRRIMLLPWQTIHFPLFLNLLSLYNG QGQLCLFFDTHSFLPFLVLLEIFQIFIFNFFNLGCLGITSSSTCTFLLRRW(+) MAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLDVLALLPAAPALFFCAVGSSGGSCFCPGK PYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLDVLALLPAAPALFFC AVGSSGGSCFCPGKPYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSLRSSKSSSLIFLTLD VLALLPAAPALFFCAVGSSGGSCFCPGKPYTFLSFLTFSPFTMAKANFASSLTPTRFFLFLSSL RSSKSSSLIFLTLDVLALLPAAPALFFCAV(+) MSWHYFQQHLHFSSAPLVLQEDHASALANHTLSSLS(+) MVCQGRSMILLKNQRRRRKVQVLLEVMPRHPRLKKLKMKIWKISRRTRKGRNEWVSKKRQSWPW PL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d047912_circ_g.3

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