Plant Circular RNA Database

Detail information of AT2G43950_circ_g.3

			
						 General Information
					
						CircRNA Name
						AT2G43950_circ_g.3
					
						ID in PlantcircBase
						ath_circ_018243
					
						Alias
						Ath_circ_FC1672
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 18201400-18201804  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT2G43950						
					
						Parent gene annotation
						
							Outer envelope pore protein 37, chloroplastic
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT2G43950_circ_g.1 AT2G43950_circ_g.2 						
					
						Support reads
						6
					
						Tissues
						inflorescences
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G43950.1:2
AT2G43950.2:2

General Information
CircRNA Name	AT2G43950_circ_g.3
ID in PlantcircBase	ath_circ_018243
Alias	Ath_circ_FC1672
Organism	Arabidpsis thaliana
Position	chr2: 18201400-18201804 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT2G43950
Parent gene annotation	Outer envelope pore protein 37, chloroplastic
Parent gene strand	-
Alternative splicing	AT2G43950_circ_g.1 AT2G43950_circ_g.2
Support reads	6
Tissues	inflorescences
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G43950.1:2 AT2G43950.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GATGGCCACTCACGTAACCAATAGGCACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGT
CAGATTTGCCTCCATAGCAACTTCTCCTTGTTGTGCcttataagcatatctcagcctcaactct
tcatctgtgtatagagcggtacaaacaccattcataacttgacgtttgaggatcccattaacag
ataaagtt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 267
Transcript exons: 18201400-18201579,18201718-18201804
CTTATAAGCATATCTCAGCCTCAACTCTTCATCTGTGTATAGAGCGGTACAAACACCATTCATA
ACTTGACGTTTGAGGATCCCATTAACAGATAAAGTTCTTTTCTGCTTCTCCTCCTCCTCCTCAT
CTTTTTCTTGTAACGAAATTTCGCCAAGTGGGAATTTAAGAGTTGCTCTTGGGTAACCAATAGG
CACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGTCAGATTTGCCTCCATAGCAACTTCT
CCTTGTTGTGC
						
						Genomic sequence
						
							CTTATAAGCATATCTCAGCCTCAACTCTTCATCTGTGTATAGAGCGGTACAAACACCATTCATA
ACTTGACGTTTGAGGATCCCATTAACAGATAAAGTTCTTTTCTGCTTCTCCTCCTCCTCCTCAT
CTTTTTCTTGTAACGAAATTTCGCCAAGTGGGAATTTAAGAGTTGCTCTTGGCTGCAGGAAGAG
TAAACCATATGGGATTATAATTAGCATTTAAAGAAAACTCCCAACCAAGCAAGAAAACAATTTA
CCGGTAATTGATACTACTGCAGTTGCAAAACCAAATAGACAATGTAAGAGATGGCCACTCACGT
AACCAATAGGCACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGTCAGATTTGCCTCCAT
AGCAACTTCTCCTTGTTGTGC

Sequences
Splice junction sequence	GATGGCCACTCACGTAACCAATAGGCACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGT CAGATTTGCCTCCATAGCAACTTCTCCTTGTTGTGCcttataagcatatctcagcctcaactct tcatctgtgtatagagcggtacaaacaccattcataacttgacgtttgaggatcccattaacag ataaagtt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 267 Transcript exons: 18201400-18201579,18201718-18201804 CTTATAAGCATATCTCAGCCTCAACTCTTCATCTGTGTATAGAGCGGTACAAACACCATTCATA ACTTGACGTTTGAGGATCCCATTAACAGATAAAGTTCTTTTCTGCTTCTCCTCCTCCTCCTCAT CTTTTTCTTGTAACGAAATTTCGCCAAGTGGGAATTTAAGAGTTGCTCTTGGGTAACCAATAGG CACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGTCAGATTTGCCTCCATAGCAACTTCT CCTTGTTGTGC
Genomic sequence	CTTATAAGCATATCTCAGCCTCAACTCTTCATCTGTGTATAGAGCGGTACAAACACCATTCATA ACTTGACGTTTGAGGATCCCATTAACAGATAAAGTTCTTTTCTGCTTCTCCTCCTCCTCCTCAT CTTTTTCTTGTAACGAAATTTCGCCAAGTGGGAATTTAAGAGTTGCTCTTGGCTGCAGGAAGAG TAAACCATATGGGATTATAATTAGCATTTAAAGAAAACTCCCAACCAAGCAAGAAAACAATTTA CCGGTAATTGATACTACTGCAGTTGCAAAACCAAATAGACAATGTAAGAGATGGCCACTCACGT AACCAATAGGCACAGGTGATGAAAGCTCCAGAGAGTAGCCAGGTTCAGTCAGATTTGCCTCCAT AGCAACTTCTCCTTGTTGTGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.25636315

Conservation Information
Conserved circRNAs	NA
PMCS	0.25636315

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18201784-18201402(-)
						
						Potential amino acid sequence
						
							MEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSVNGILKRQVMNG
VCTALYTDEELRLRYAYKAQQGEVAMEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEK
DEEEEEKQKRTLSVNGILKRQVMNGVCTALYTDEELRLRYAYKAQQGEVAMEANLTEPGYSLEL
SSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSVNGILKRQVMNGVCTALYTDEELRLR
YAYKAQQGEVAMEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSV
NGILKRQVMNGVCTALYTDEELRLRYAYK(-)
						
						Sponge-miRNAs
						
							ath-miR854b ath-miR854d 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	18201784-18201402(-)
Potential amino acid sequence	MEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSVNGILKRQVMNG VCTALYTDEELRLRYAYKAQQGEVAMEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEK DEEEEEKQKRTLSVNGILKRQVMNGVCTALYTDEELRLRYAYKAQQGEVAMEANLTEPGYSLEL SSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSVNGILKRQVMNGVCTALYTDEELRLR YAYKAQQGEVAMEANLTEPGYSLELSSPVPIGYPRATLKFPLGEISLQEKDEEEEEKQKRTLSV NGILKRQVMNGVCTALYTDEELRLRYAYK(-)
Sponge-miRNAs	ath-miR854b ath-miR854d
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT2G43950_circ_g.3

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