Plant Circular RNA Database

Detail information of AT5G18590_circ_g.8

			
						 General Information
					
						CircRNA Name
						AT5G18590_circ_g.8
					
						ID in PlantcircBase
						ath_circ_039029
					
						Alias
						At_ciR444, AT5G18590_C2
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 6180209-6180926  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, CIRI, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT5G18590						
					
						Parent gene annotation
						
							Galactose oxidase/kelch repeat superfamily protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G18590_circ_g.2 AT5G18590_circ_g.3 AT5G18590_circ_g.4 AT5G18590_circ_g.5 AT5G18590_circ_g.6 AT5G18590_circ_g.7 AT5G18590_circ_g.9 AT5G18590_circ_g.10 						
					
						Support reads
						17/4
					
						Tissues
						leaf/root, leaf, aerial, inflorescences, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G18590.1:4
AT5G18590.2:4

General Information
CircRNA Name	AT5G18590_circ_g.8
ID in PlantcircBase	ath_circ_039029
Alias	At_ciR444, AT5G18590_C2
Organism	Arabidpsis thaliana
Position	chr5: 6180209-6180926 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, CIRI, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT5G18590
Parent gene annotation	Galactose oxidase/kelch repeat superfamily protein
Parent gene strand	-
Alternative splicing	AT5G18590_circ_g.2 AT5G18590_circ_g.3 AT5G18590_circ_g.4 AT5G18590_circ_g.5 AT5G18590_circ_g.6 AT5G18590_circ_g.7 AT5G18590_circ_g.9 AT5G18590_circ_g.10
Support reads	17/4
Tissues	leaf/root, leaf, aerial, inflorescences, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G18590.1:4 AT5G18590.2:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GGTGATGGGTGAAATCCTCTTATC
 R: GAGCGGGTAGTTGTGGAGTTC
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GGTGATGGGTGAAATCCTCTTATC R: GAGCGGGTAGTTGTGGAGTTC PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CACAAACTTCTAAATGTATAATGAGGACTGAGAGTTACCGGTAAGTCTCCTTTTGCATCCATTA
GTGACCAGCATTCCGAATCAGTATCAAATGCCCACAtcttctttctgcttcctcctccggttat
ataccatttagtaccgcaaagaactccacaactacccgctcttggtgatgggtgaaatcctctt
atctttat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 444
Transcript exons: 6180209-6180323,6180403-6180529,6180624-6180763,6180865-
6180926
TCTTCTTTCTGCTTCCTCCTCCGGTTATATACCATTTAGTACCGCAAAGAACTCCACAACTACC
CGCTCTTGGTGATGGGTGAAATCCTCTTATCTTTATTCTTGACCATACCATCGTTTCAAAGTCG
AGTGAGTATAAGTCATTTAGTGTTTTGTTCTTGCCCGAACCTCCAAACACAAACAGAATTTTGT
CGTCGAATAACGTGGCTACATGATGTGACCTTGCACACGGCCGTGTTCCTGTGCAATTAAGAGG
AAGCCATGTGGATGATTTGAGATCAAACATATGAAGATCGTTTAGTTTCCTCTTCTTAGAGTCT
TCACCACCGAATAGTATCAATACCGAACTAGCCCTCACCACTGTGTGACCGCTGCGACTGACCG
GTAAGTCTCCTTTTGCATCCATTAGTGACCAGCATTCCGAATCAGTATCAAATGCCCACA
						
						Genomic sequence
						
							TCTTCTTTCTGCTTCCTCCTCCGGTTATATACCATTTAGTACCGCAAAGAACTCCACAACTACC
CGCTCTTGGTGATGGGTGAAATCCTCTTATCTTTATTCTTGACCATACCATCTTGATGACACCA
AGAGATAAGAGATGTTCAATGTCAAGAAACCAAATAAATAAAAAACATTATAGCAATAAGTATT
ACCGTTTCAAAGTCGAGTGAGTATAAGTCATTTAGTGTTTTGTTCTTGCCCGAACCTCCAAACA
CAAACAGAATTTTGTCGTCGAATAACGTGGCTACATGATGTGACCTTGCACACGGCCGTGTTCC
TCTGTTTTTTTAATGAGAAGAACCGTATCAAGAAATCACAAGTAGGAGTTCAATGTAAACAATC
ATCAGAAAAATGTTAAAAAGTAAAAACTCACGTGCAATTAAGAGGAAGCCATGTGGATGATTTG
AGATCAAACATATGAAGATCGTTTAGTTTCCTCTTCTTAGAGTCTTCACCACCGAATAGTATCA
ATACCGAACTAGCCCTCACCACTGTGTGACCGCTGCGACTGACCTGCATTTTCATATCCAAATC
AGATTTTTTTCTCAAGTCACTTCACAATCCAAACCATAAAGCCACAAACTTCTAAATGTATAAT
GAGGACTGAGAGTTACCGGTAAGTCTCCTTTTGCATCCATTAGTGACCAGCATTCCGAATCAGT
ATCAAATGCCCACA

Sequences
Splice junction sequence	CACAAACTTCTAAATGTATAATGAGGACTGAGAGTTACCGGTAAGTCTCCTTTTGCATCCATTA GTGACCAGCATTCCGAATCAGTATCAAATGCCCACAtcttctttctgcttcctcctccggttat ataccatttagtaccgcaaagaactccacaactacccgctcttggtgatgggtgaaatcctctt atctttat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 444 Transcript exons: 6180209-6180323,6180403-6180529,6180624-6180763,6180865- 6180926 TCTTCTTTCTGCTTCCTCCTCCGGTTATATACCATTTAGTACCGCAAAGAACTCCACAACTACC CGCTCTTGGTGATGGGTGAAATCCTCTTATCTTTATTCTTGACCATACCATCGTTTCAAAGTCG AGTGAGTATAAGTCATTTAGTGTTTTGTTCTTGCCCGAACCTCCAAACACAAACAGAATTTTGT CGTCGAATAACGTGGCTACATGATGTGACCTTGCACACGGCCGTGTTCCTGTGCAATTAAGAGG AAGCCATGTGGATGATTTGAGATCAAACATATGAAGATCGTTTAGTTTCCTCTTCTTAGAGTCT TCACCACCGAATAGTATCAATACCGAACTAGCCCTCACCACTGTGTGACCGCTGCGACTGACCG GTAAGTCTCCTTTTGCATCCATTAGTGACCAGCATTCCGAATCAGTATCAAATGCCCACA
Genomic sequence	TCTTCTTTCTGCTTCCTCCTCCGGTTATATACCATTTAGTACCGCAAAGAACTCCACAACTACC CGCTCTTGGTGATGGGTGAAATCCTCTTATCTTTATTCTTGACCATACCATCTTGATGACACCA AGAGATAAGAGATGTTCAATGTCAAGAAACCAAATAAATAAAAAACATTATAGCAATAAGTATT ACCGTTTCAAAGTCGAGTGAGTATAAGTCATTTAGTGTTTTGTTCTTGCCCGAACCTCCAAACA CAAACAGAATTTTGTCGTCGAATAACGTGGCTACATGATGTGACCTTGCACACGGCCGTGTTCC TCTGTTTTTTTAATGAGAAGAACCGTATCAAGAAATCACAAGTAGGAGTTCAATGTAAACAATC ATCAGAAAAATGTTAAAAAGTAAAAACTCACGTGCAATTAAGAGGAAGCCATGTGGATGATTTG AGATCAAACATATGAAGATCGTTTAGTTTCCTCTTCTTAGAGTCTTCACCACCGAATAGTATCA ATACCGAACTAGCCCTCACCACTGTGTGACCGCTGCGACTGACCTGCATTTTCATATCCAAATC AGATTTTTTTCTCAAGTCACTTCACAATCCAAACCATAAAGCCACAAACTTCTAAATGTATAAT GAGGACTGAGAGTTACCGGTAAGTCTCCTTTTGCATCCATTAGTGACCAGCATTCCGAATCAGT ATCAAATGCCCACA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.493737513

Conservation Information
Conserved circRNAs	NA
PMCS	0.493737513

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6180889-6180211(-)
						
						Potential amino acid sequence
						
							MDAKGDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSH
HVATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYIT
GGGSRKKMWAFDTDSECWSLMDAKGDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDL
KSSTWLPLNCTGTRPCARSHHVATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGF
HPSPRAGSCGVLCGTKWYITGGGSRKKMWAFDTDSECWSLMDAKGDLPVSRSGHTVVRASSVLI
LFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHVATLFDDKILFVFGGSGKNKTLN
DLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYITGGGSRKKMWAFDTDSECWSLMDAK
GDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHVAT
LFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYITGGGS
RKK(-)
						
						Sponge-miRNAs
						
							ath-miR5021 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						regulation of development and senescence of leaves

Functional Information
Coding potential	Y
Potential coding position	6180889-6180211(-)
Potential amino acid sequence	MDAKGDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSH HVATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYIT GGGSRKKMWAFDTDSECWSLMDAKGDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDL KSSTWLPLNCTGTRPCARSHHVATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGF HPSPRAGSCGVLCGTKWYITGGGSRKKMWAFDTDSECWSLMDAKGDLPVSRSGHTVVRASSVLI LFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHVATLFDDKILFVFGGSGKNKTLN DLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYITGGGSRKKMWAFDTDSECWSLMDAK GDLPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHVAT LFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPRAGSCGVLCGTKWYITGGGS RKK(-)
Sponge-miRNAs	ath-miR5021
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	regulation of development and senescence of leaves

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Liu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT5G18590_circ_g.8

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