Plant Circular RNA Database

Detail information of Os09g0563300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os09g0563300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_040200
					
						Alias
						Os_ciR11946
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr9: 22384305-22384961  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os09g0563300						
					
						Parent gene annotation
						
							Similar to GroEL-like chaperone, ATPase. (Os09t0563300-01);Alpha
 subunit of chaperonin protein 60, Chloroplast development at ea
rly leaf stage (Os09t0563300-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os09g0563300_circ_g.2 Os09g0563300_circ_g.3 Os09g0563300_circ_g.4 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os09t0563300-02:2
Os09t0563300-01:2

General Information
CircRNA Name	Os09g0563300_circ_g.1
ID in PlantcircBase	osa_circ_040200
Alias	Os_ciR11946
Organism	Oryza sativa
Position	chr9: 22384305-22384961 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	find_circ
Parent gene	Os09g0563300
Parent gene annotation	Similar to GroEL-like chaperone, ATPase. (Os09t0563300-01);Alpha subunit of chaperonin protein 60, Chloroplast development at ea rly leaf stage (Os09t0563300-02)
Parent gene strand	+
Alternative splicing	Os09g0563300_circ_g.2 Os09g0563300_circ_g.3 Os09g0563300_circ_g.4
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os09t0563300-02:2 Os09t0563300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTAACAAACTGAATGGACTGCTAAATGTTGCTGTTGTTAAATGTCCTGGTTTGGGAGATGAAA
AGAAGGCTATTCTCCAAGATATTGCTATTATGACAGctgttgcttcaatatcagctggcaatga
tgaatatgttggcgatctcattgctgatgcattggagaagataggtcctgatggaataattaaa
attgagtc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 453
Transcript exons: 22384305-22384450,22384655-22384961
CTGTTGCTTCAATATCAGCTGGCAATGATGAATATGTTGGCGATCTCATTGCTGATGCATTGGA
GAAGATAGGTCCTGATGGAATAATTAAAATTGAGTCCTCATCTTCAATATATACTTCAGTTGAG
GTGCAAGAAGGAATGAAGATAGACAAAGGTTATATCTCTCCACATTTCATCACAAATCAAGACA
AAGCAATTGTTGAATTTGAAAATGCTAGGGTACTTTTAACTGATCAGCGGGTGGATGATGTCCA
AGAGATACTTCCTTTGCTAGAGAAGACCACACAGTTGAGTGTCCCTTTGTTGATTATTGCGGAG
GATGTTTCACATACGGTGTATTCAACTCTTGTTCTTAACAAACTGAATGGACTGCTAAATGTTG
CTGTTGTTAAATGTCCTGGTTTGGGAGATGAAAAGAAGGCTATTCTCCAAGATATTGCTATTAT
GACAG
						
						Genomic sequence
						
							CTGTTGCTTCAATATCAGCTGGCAATGATGAATATGTTGGCGATCTCATTGCTGATGCATTGGA
GAAGATAGGTCCTGATGGAATAATTAAAATTGAGTCCTCATCTTCAATATATACTTCAGTTGAG
GTGCAAGAAGGAATGAAGGTATGTTTATGTGGATATGAAATTTCTCCCTACAATATGCTACCTG
TATGCAAAGAACTTCCCTTTTGCAACAAAATGCAGCTATGTCTCAGGGTGTGTCAAACAAAATC
ATTCCATTGAACCACTAATCAACATTTAGTGCAGAAAGTAGCTGGTGTAAATTTATAGTACTAT
GGTTCATGATTCTCCGTCTGGTCGTTGCAGATAGACAAAGGTTATATCTCTCCACATTTCATCA
CAAATCAAGACAAAGCAATTGTTGAATTTGAAAATGCTAGGGTACTTTTAACTGATCAGCGGGT
GGATGATGTCCAAGAGATACTTCCTTTGCTAGAGAAGACCACACAGTTGAGTGTCCCTTTGTTG
ATTATTGCGGAGGATGTTTCACATACGGTGTATTCAACTCTTGTTCTTAACAAACTGAATGGAC
TGCTAAATGTTGCTGTTGTTAAATGTCCTGGTTTGGGAGATGAAAAGAAGGCTATTCTCCAAGA
TATTGCTATTATGACAG

Sequences
Splice junction sequence	CTTAACAAACTGAATGGACTGCTAAATGTTGCTGTTGTTAAATGTCCTGGTTTGGGAGATGAAA AGAAGGCTATTCTCCAAGATATTGCTATTATGACAGctgttgcttcaatatcagctggcaatga tgaatatgttggcgatctcattgctgatgcattggagaagataggtcctgatggaataattaaa attgagtc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 453 Transcript exons: 22384305-22384450,22384655-22384961 CTGTTGCTTCAATATCAGCTGGCAATGATGAATATGTTGGCGATCTCATTGCTGATGCATTGGA GAAGATAGGTCCTGATGGAATAATTAAAATTGAGTCCTCATCTTCAATATATACTTCAGTTGAG GTGCAAGAAGGAATGAAGATAGACAAAGGTTATATCTCTCCACATTTCATCACAAATCAAGACA AAGCAATTGTTGAATTTGAAAATGCTAGGGTACTTTTAACTGATCAGCGGGTGGATGATGTCCA AGAGATACTTCCTTTGCTAGAGAAGACCACACAGTTGAGTGTCCCTTTGTTGATTATTGCGGAG GATGTTTCACATACGGTGTATTCAACTCTTGTTCTTAACAAACTGAATGGACTGCTAAATGTTG CTGTTGTTAAATGTCCTGGTTTGGGAGATGAAAAGAAGGCTATTCTCCAAGATATTGCTATTAT GACAG
Genomic sequence	CTGTTGCTTCAATATCAGCTGGCAATGATGAATATGTTGGCGATCTCATTGCTGATGCATTGGA GAAGATAGGTCCTGATGGAATAATTAAAATTGAGTCCTCATCTTCAATATATACTTCAGTTGAG GTGCAAGAAGGAATGAAGGTATGTTTATGTGGATATGAAATTTCTCCCTACAATATGCTACCTG TATGCAAAGAACTTCCCTTTTGCAACAAAATGCAGCTATGTCTCAGGGTGTGTCAAACAAAATC ATTCCATTGAACCACTAATCAACATTTAGTGCAGAAAGTAGCTGGTGTAAATTTATAGTACTAT GGTTCATGATTCTCCGTCTGGTCGTTGCAGATAGACAAAGGTTATATCTCTCCACATTTCATCA CAAATCAAGACAAAGCAATTGTTGAATTTGAAAATGCTAGGGTACTTTTAACTGATCAGCGGGT GGATGATGTCCAAGAGATACTTCCTTTGCTAGAGAAGACCACACAGTTGAGTGTCCCTTTGTTG ATTATTGCGGAGGATGTTTCACATACGGTGTATTCAACTCTTGTTCTTAACAAACTGAATGGAC TGCTAAATGTTGCTGTTGTTAAATGTCCTGGTTTGGGAGATGAAAAGAAGGCTATTCTCCAAGA TATTGCTATTATGACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.325303881

Conservation Information
Conserved circRNAs	NA
PMCS	0.325303881

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							22384446-22384958(+)
22384355-22384746(-)
						
						Potential amino acid sequence
						
							MKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIAEDVSH
TVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMTAVASISAGNDEYVGDLIADALEKI
GPDGIIKIESSSSIYTSVEVQEGMKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEI
LPLLEKTTQLSVPLLIIAEDVSHTVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMTA
VASISAGNDEYVGDLIADALEKIGPDGIIKIESSSSIYTSVEVQEGMKIDKGYISPHFITNQDK
AIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIAEDVSHTVYSTLVLNKLNGLLNVA
VVKCPGLGDEKKAILQDIAIMTAVASISAGNDEYVGDLIADALEKIGPDGIIKIESSSSIYTSV
EVQEGMKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIA
EDVSHTVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMT(+)
MRSPTYSSLPADIEATAVIIAISWRIAFFSSPKPGHLTTATFSSPFSLLRTRVEYTVCETSSAI
INKGTLNCVVFSSKGSISWTSSTR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	22384446-22384958(+) 22384355-22384746(-)
Potential amino acid sequence	MKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIAEDVSH TVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMTAVASISAGNDEYVGDLIADALEKI GPDGIIKIESSSSIYTSVEVQEGMKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEI LPLLEKTTQLSVPLLIIAEDVSHTVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMTA VASISAGNDEYVGDLIADALEKIGPDGIIKIESSSSIYTSVEVQEGMKIDKGYISPHFITNQDK AIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIAEDVSHTVYSTLVLNKLNGLLNVA VVKCPGLGDEKKAILQDIAIMTAVASISAGNDEYVGDLIADALEKIGPDGIIKIESSSSIYTSV EVQEGMKIDKGYISPHFITNQDKAIVEFENARVLLTDQRVDDVQEILPLLEKTTQLSVPLLIIA EDVSHTVYSTLVLNKLNGLLNVAVVKCPGLGDEKKAILQDIAIMT(+) MRSPTYSSLPADIEATAVIIAISWRIAFFSSPKPGHLTTATFSSPFSLLRTRVEYTVCETSSAI INKGTLNCVVFSSKGSISWTSSTR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os09g0563300_circ_g.1

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