Plant Circular RNA Database

Detail information of Os03g0586300_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0586300_circ_g.2
					
						ID in PlantcircBase
						osa_circ_020545
					
						Alias
						Os_ciR8652
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 21625506-21627389  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0586300						
					
						Parent gene annotation
						
							Reticulon family protein. (Os03t0586300-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0586300_circ_g.1 						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0586300-01:5

General Information
CircRNA Name	Os03g0586300_circ_g.2
ID in PlantcircBase	osa_circ_020545
Alias	Os_ciR8652
Organism	Oryza sativa
Position	chr3: 21625506-21627389 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0586300
Parent gene annotation	Reticulon family protein. (Os03t0586300-01)
Parent gene strand	-
Alternative splicing	Os03g0586300_circ_g.1
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0586300-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTATCATCAGATAAGAGTAAGTCTTTCCAATCTTTTGCCAGCCTCTTAGCTGGTTCAGTGGGTC
GCCAATGGAAAATACCGATATTGTATGCACCAGTAActcaaaaacatgatccattggacagagg
aaaggttgtctggtcattgttccttccaaaactccaggatgtccaaaccacatcctatcaagcc
tgcaccat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 640
Transcript exons: 21625506-21625596,21626138-21626191,21626299-21626393,21
626747-21626889,21627133-21627389
CTCAAAAACATGATCCATTGGACAGAGGAAAGGTTGTCTGGTCATTGTTCCTTCCAAAACTCCA
GGATGTCCAAACCACATCCTATCAAGCCAGGGTCCTCTTTAATAGGGAAGCAATAGCGAGTGCA
GTTCTTATTTGCTTCAACTGGTAATTGACCAATGCAAAATGAGATTTAACAGTATGAGCGCCAT
CTAACAGCAAGCTTTTAGGAATGTTGGGTCTAAATGATAGGAAACCTCCTGGTGAGTCATAGTA
TGATGGCTGATCAAAGAAAAGCATTGCTTCACGCAATCTATGACGCTTTCCCACAGTACCTGCA
TACTGGAAAGTAGTATGCACAGCATATGGTTCTAGCCGAAGCTGCTGATACATGCCCTGGCAAC
TGCACAAAATATGTATGACCACTACAGAATATACTTGCTGGTAGGACTCCAAGCTTCAGTTTCC
CATCATAAGAATATACAAGTTCATCTTCTCCAACAACCGGGTATCCAAATTTTTTGTGTATAAG
GTCATTAAAAGCATTCTGGTCCCACAACGTATCATCAGATAAGAGTAAGTCTTTCCAATCTTTT
GCCAGCCTCTTAGCTGGTTCAGTGGGTCGCCAATGGAAAATACCGATATTGTATGCACCAGTAA
						
					

						Genomic sequence
						
							CTCAAAAACATGATCCATTGGACAGAGGAAAGGTTGTCTGGTCATTGTTCCTTCCAAAACTCCA
GGATGTCCAAACCACATCCTATCAAGCCTGCACCATAATGGAGGCATTACCTAAAAGTACAAAC
AGTAGTCAGAAATTGGGTGTCCACAAGTTTGAGCACAATACATCGAACAGCGTAATTCTGCTCT
CAAACACCTCCAGAAGTTATCTAAGATAAAAACTTATCCTCAAAAAAGCAAGCATGAAGCACCT
ACCATACATTACTACTCAACACCTCAATACTCACTAATTAGCTGCAGCTTACTCATTTAAGGAT
AAATGCACTTTTGCAAGTTGTAAATTTTGTATCAAATTAAGATGGTGCACACATCATCAGTAAA
GACAAAATTAACATCTAAAAGATAAGAACATATTAATAATCATTTCAACCTTTTAACATATAGA
AAGTTTATGGAAAGAGATCACATTCAAGAAATCGAATTGCAGGTTAAATCAATCTCAACTAAAT
GTTTACACTTAATGATCTGCATGTCCACTACCTCACAATCACGAATAGTAAGTGTGTAGTATTC
ACACTACCATCATGGAGTTCAAATCAAACTAGAAAAAGAATAGAAATTCCACTGACCAGGGTCC
TCTTTAATAGGGAAGCAATAGCGAGTGCAGTTCTTATTTGCTTCAACTGAAAATTTTCAAGCAC
ATGACTAATCAGGAAAAAGAAAAGGAATAACCATCAATAACAGAAGTGCAGCCACAAATTTGCA
GAAATATATGTGTAGCTGCAGATACCTGGTAATTGACCAATGCAAAATGAGATTTAACAGTATG
AGCGCCATCTAACAGCAAGCTTTTAGGAATGTTGGGTCTAAATGATAGGAAACCTCCTGCATCA
TAATACCAGGTAAATTATGGACATGATGAAAAAAAGTAGTAAGTAAAGATTGTAGGATTCAAAT
CCGAATAATGTTATATGCATGAAAATAATTTCATGTGACCATCTTTTCATTCTTGGATCATATA
TGGCTACAGCCTACATGGTTTGTTTAACAGTGCTCCATTGGTAATTCTGAGATAACCCATACAG
ACGAAACATTTTAACACTAGGGTACATCATGGTCTTCAAACATAAAATCAGAAGGCATTTGTAC
ACTAGAACACATCATGATCTTTAAACATGAAGTCATGAACCTCTGTCCCAAAGAAGTTCCGTCA
TGATAGGGTCAGAGAATAAACATACCTGGTGAGTCATAGTATGATGGCTGATCAAAGAAAAGCA
TTGCTTCACGCAATCTATGACGCTTTCCCACAGTACCTGCATACTGGAAAGTAGTATGCACAGC
ATATGGTTCTAGCCGAAGCTGCTGATACATGCCCTGGCAACTCAAGAAAAAAATACATTTCAGG
TATAAACAACCCAAGAAAATTACAGAGAATTTTAGACGAAAGGGACAGTATAAACAAGATGCAC
AAAGTGTGTGAAACAAAAGAACAAGAACCAAACATATGCAGGACAACAAAGATAAAAAATGCAA
TTGCTAACTAACTGAAAATTTACTCACCAAAGAAAAATTTTGACATGCAAGATATTTAATTTGC
CAATTTCAAACAAATAAGAGCAACTACCTGCACAAAATATGTATGACCACTACAGAATATACTT
GCTGGTAGGACTCCAAGCTTCAGTTTCCCATCATAAGAATATACAAGTTCATCTTCTCCAACAA
CCGGGTATCCAAATTTTTTGTGTATAAGGTCATTAAAAGCATTCTGGTCCCACAACGTATCATC
AGATAAGAGTAAGTCTTTCCAATCTTTTGCCAGCCTCTTAGCTGGTTCAGTGGGTCGCCAATGG
AAAATACCGATATTGTATGCACCAGTAA
						
					

			
		

Sequences
Splice junction sequence	GTATCATCAGATAAGAGTAAGTCTTTCCAATCTTTTGCCAGCCTCTTAGCTGGTTCAGTGGGTC GCCAATGGAAAATACCGATATTGTATGCACCAGTAActcaaaaacatgatccattggacagagg aaaggttgtctggtcattgttccttccaaaactccaggatgtccaaaccacatcctatcaagcc tgcaccat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 640 Transcript exons: 21625506-21625596,21626138-21626191,21626299-21626393,21 626747-21626889,21627133-21627389 CTCAAAAACATGATCCATTGGACAGAGGAAAGGTTGTCTGGTCATTGTTCCTTCCAAAACTCCA GGATGTCCAAACCACATCCTATCAAGCCAGGGTCCTCTTTAATAGGGAAGCAATAGCGAGTGCA GTTCTTATTTGCTTCAACTGGTAATTGACCAATGCAAAATGAGATTTAACAGTATGAGCGCCAT CTAACAGCAAGCTTTTAGGAATGTTGGGTCTAAATGATAGGAAACCTCCTGGTGAGTCATAGTA TGATGGCTGATCAAAGAAAAGCATTGCTTCACGCAATCTATGACGCTTTCCCACAGTACCTGCA TACTGGAAAGTAGTATGCACAGCATATGGTTCTAGCCGAAGCTGCTGATACATGCCCTGGCAAC TGCACAAAATATGTATGACCACTACAGAATATACTTGCTGGTAGGACTCCAAGCTTCAGTTTCC CATCATAAGAATATACAAGTTCATCTTCTCCAACAACCGGGTATCCAAATTTTTTGTGTATAAG GTCATTAAAAGCATTCTGGTCCCACAACGTATCATCAGATAAGAGTAAGTCTTTCCAATCTTTT GCCAGCCTCTTAGCTGGTTCAGTGGGTCGCCAATGGAAAATACCGATATTGTATGCACCAGTAA
Genomic sequence	CTCAAAAACATGATCCATTGGACAGAGGAAAGGTTGTCTGGTCATTGTTCCTTCCAAAACTCCA GGATGTCCAAACCACATCCTATCAAGCCTGCACCATAATGGAGGCATTACCTAAAAGTACAAAC AGTAGTCAGAAATTGGGTGTCCACAAGTTTGAGCACAATACATCGAACAGCGTAATTCTGCTCT CAAACACCTCCAGAAGTTATCTAAGATAAAAACTTATCCTCAAAAAAGCAAGCATGAAGCACCT ACCATACATTACTACTCAACACCTCAATACTCACTAATTAGCTGCAGCTTACTCATTTAAGGAT AAATGCACTTTTGCAAGTTGTAAATTTTGTATCAAATTAAGATGGTGCACACATCATCAGTAAA GACAAAATTAACATCTAAAAGATAAGAACATATTAATAATCATTTCAACCTTTTAACATATAGA AAGTTTATGGAAAGAGATCACATTCAAGAAATCGAATTGCAGGTTAAATCAATCTCAACTAAAT GTTTACACTTAATGATCTGCATGTCCACTACCTCACAATCACGAATAGTAAGTGTGTAGTATTC ACACTACCATCATGGAGTTCAAATCAAACTAGAAAAAGAATAGAAATTCCACTGACCAGGGTCC TCTTTAATAGGGAAGCAATAGCGAGTGCAGTTCTTATTTGCTTCAACTGAAAATTTTCAAGCAC ATGACTAATCAGGAAAAAGAAAAGGAATAACCATCAATAACAGAAGTGCAGCCACAAATTTGCA GAAATATATGTGTAGCTGCAGATACCTGGTAATTGACCAATGCAAAATGAGATTTAACAGTATG AGCGCCATCTAACAGCAAGCTTTTAGGAATGTTGGGTCTAAATGATAGGAAACCTCCTGCATCA TAATACCAGGTAAATTATGGACATGATGAAAAAAAGTAGTAAGTAAAGATTGTAGGATTCAAAT CCGAATAATGTTATATGCATGAAAATAATTTCATGTGACCATCTTTTCATTCTTGGATCATATA TGGCTACAGCCTACATGGTTTGTTTAACAGTGCTCCATTGGTAATTCTGAGATAACCCATACAG ACGAAACATTTTAACACTAGGGTACATCATGGTCTTCAAACATAAAATCAGAAGGCATTTGTAC ACTAGAACACATCATGATCTTTAAACATGAAGTCATGAACCTCTGTCCCAAAGAAGTTCCGTCA TGATAGGGTCAGAGAATAAACATACCTGGTGAGTCATAGTATGATGGCTGATCAAAGAAAAGCA TTGCTTCACGCAATCTATGACGCTTTCCCACAGTACCTGCATACTGGAAAGTAGTATGCACAGC ATATGGTTCTAGCCGAAGCTGCTGATACATGCCCTGGCAACTCAAGAAAAAAATACATTTCAGG TATAAACAACCCAAGAAAATTACAGAGAATTTTAGACGAAAGGGACAGTATAAACAAGATGCAC AAAGTGTGTGAAACAAAAGAACAAGAACCAAACATATGCAGGACAACAAAGATAAAAAATGCAA TTGCTAACTAACTGAAAATTTACTCACCAAAGAAAAATTTTGACATGCAAGATATTTAATTTGC CAATTTCAAACAAATAAGAGCAACTACCTGCACAAAATATGTATGACCACTACAGAATATACTT GCTGGTAGGACTCCAAGCTTCAGTTTCCCATCATAAGAATATACAAGTTCATCTTCTCCAACAA CCGGGTATCCAAATTTTTTGTGTATAAGGTCATTAAAAGCATTCTGGTCCCACAACGTATCATC AGATAAGAGTAAGTCTTTCCAATCTTTTGCCAGCCTCTTAGCTGGTTCAGTGGGTCGCCAATGG AAAATACCGATATTGTATGCACCAGTAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.2359283

Conservation Information
Conserved circRNAs	NA
PMCS	0.2359283

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							21625588-21627191(-)
						
						Potential amino acid sequence
						
							MWFGHPGVLEGTMTRQPFLCPMDHVFELLVHTISVFSIGDPLNQLRGWQKIGKTYSYLMIRCGT
RMLLMTLYTKNLDTRLLEKMNLYILMMGN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	21625588-21627191(-)
Potential amino acid sequence	MWFGHPGVLEGTMTRQPFLCPMDHVFELLVHTISVFSIGDPLNQLRGWQKIGKTYSYLMIRCGT RMLLMTLYTKNLDTRLLEKMNLYILMMGN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0586300_circ_g.2

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