Plant Circular RNA Database

Detail information of Os02g0100333_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0100333_circ_g.2
					
						ID in PlantcircBase
						osa_circ_012572
					
						Alias
						Os02circ00022/Os_ciR2166
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 14379-14549  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ie-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os02g0100333						
					
						Parent gene annotation
						
							Hypothetical gene. (Os02t0100333-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0100333_circ_g.1 Os02g0100333_circ_g.3 Os02g0100333_circ_g.4 Os02g0100333_circ_g.5 Os02g0100333_circ_g.6 Os02g0100333_circ_g.7 						
					
						Support reads
						2/6/1
					
						Tissues
						leaf/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0100300-01:1
Os02t0100333-00:1
Os02t0100300-01:1

General Information
CircRNA Name	Os02g0100333_circ_g.2
ID in PlantcircBase	osa_circ_012572
Alias	Os02circ00022/Os_ciR2166
Organism	Oryza sativa
Position	chr2: 14379-14549 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ie-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os02g0100333
Parent gene annotation	Hypothetical gene. (Os02t0100333-00)
Parent gene strand	-
Alternative splicing	Os02g0100333_circ_g.1 Os02g0100333_circ_g.3 Os02g0100333_circ_g.4 Os02g0100333_circ_g.5 Os02g0100333_circ_g.6 Os02g0100333_circ_g.7
Support reads	2/6/1
Tissues	leaf/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0100300-01:1 Os02t0100333-00:1 Os02t0100300-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGAAGGAGACATTGTCGAATCTGAC
CATCAGGACCCTCCGCAAAAGccgtcctccatggatgacatcgacgatgaatacgacgacgacg
acgacgacgatctcaacatgagggatgccactccggagccgga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 144
Transcript exons: 14379-14489,14517-14549
CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA
GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGGTCGAATCTGACCATCA
GGACCCTCCGCAAAAG
Transcript length: 151
Transcript exons: 14379-14443,14464-14549
CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA
GACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGAAGGAGACATTGTCGAATCTG
ACCATCAGGACCCTCCGCAAAAG
Transcript length: 171
Transcript exons: 14379-14549
CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA
GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGA
AGGAGACATTGTCGAATCTGACCATCAGGACCCTCCGCAAAAG
						
						Genomic sequence
						
							CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA
GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGA
AGGAGACATTGTCGAATCTGACCATCAGGACCCTCCGCAAAAG

Sequences
Splice junction sequence	CGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGAAGGAGACATTGTCGAATCTGAC CATCAGGACCCTCCGCAAAAGccgtcctccatggatgacatcgacgatgaatacgacgacgacg acgacgacgatctcaacatgagggatgccactccggagccgga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 144 Transcript exons: 14379-14489,14517-14549 CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGGTCGAATCTGACCATCA GGACCCTCCGCAAAAG Transcript length: 151 Transcript exons: 14379-14443,14464-14549 CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA GACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGAAGGAGACATTGTCGAATCTG ACCATCAGGACCCTCCGCAAAAG Transcript length: 171 Transcript exons: 14379-14549 CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGA AGGAGACATTGTCGAATCTGACCATCAGGACCCTCCGCAAAAG
Genomic sequence	CCGTCCTCCATGGATGACATCGACGATGAATACGACGACGACGACGACGACGATCTCAACATGA GGGATGCCACTCCGGAGCCGGACGAGCTTGACCAGGACATCGTCGAGTCCGATCTTGAACTTGA AGGAGACATTGTCGAATCTGACCATCAGGACCCTCCGCAAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.623941172

Conservation Information
Conserved circRNAs	NA
PMCS	0.623941172

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							14389-14546(+)
14447-14439(+)
14533-14381(-)
14517-14533(-)
						
						Potential amino acid sequence
						
							MDDIDDEYDDDDDDDLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDE
YDDDDDDDLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDEYDDDDDD
DLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDEYDDDDDDDLNMRDA
TPEPDELDQDIVESDLELEGDIVESDHQDPPQK(+)
MPLRSRTSLTRTSSSPILNLKETLSNLTIRTLRKSRPPWMTSTMNTTTTTTTIST*(+)
MVRFDNVSFKFKIGLDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNV
SFKFKIGLDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNVSFKFKIG
LDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNVSFKFKIGLDDVLVK
LVRLRSGIPHVEIVVVVVVVFIVDVIHGGR(-)
MSPSSSRSDSTMSWSSSSGSGVASLMLRSSSSSSSYSSSMSSMEDGFCGGS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	14389-14546(+) 14447-14439(+) 14533-14381(-) 14517-14533(-)
Potential amino acid sequence	MDDIDDEYDDDDDDDLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDE YDDDDDDDLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDEYDDDDDD DLNMRDATPEPDELDQDIVESDLELEGDIVESDHQDPPQKPSSMDDIDDEYDDDDDDDLNMRDA TPEPDELDQDIVESDLELEGDIVESDHQDPPQK(+) MPLRSRTSLTRTSSSPILNLKETLSNLTIRTLRKSRPPWMTSTMNTTTTTTTIST(+) MVRFDNVSFKFKIGLDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNV SFKFKIGLDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNVSFKFKIG LDDVLVKLVRLRSGIPHVEIVVVVVVVFIVDVIHGGRLLRRVLMVRFDNVSFKFKIGLDDVLVK LVRLRSGIPHVEIVVVVVVVFIVDVIHGGR(-) MSPSSSRSDSTMSWSSSSGSGVASLMLRSSSSSSSYSSSMSSMEDGFCGGS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0100333_circ_g.2

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