Plant Circular RNA Database

Detail information of orai.003G158800_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.003G158800_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000280
					
						Alias
						Chr03:42805727|42806490
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr03: 42805727-42806490  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.003G158800						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.003G158800_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.003G158800.1:3
Gorai.003G158800.2:3
Gorai.003G158800.6:3
Gorai.003G158800.4:3
Gorai.003G158800.3:3
Gorai.003G158800.5:3

General Information
CircRNA Name	orai.003G158800_circ_g.1
ID in PlantcircBase	gra_circ_000280
Alias	Chr03:42805727\|42806490
Organism	Gossypium raimondii
Position	chrChr03: 42805727-42806490 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.003G158800
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.003G158800_circ_g.2
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.003G158800.1:3 Gorai.003G158800.2:3 Gorai.003G158800.6:3 Gorai.003G158800.4:3 Gorai.003G158800.3:3 Gorai.003G158800.5:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CATAAGTACTTTGAAGTTTGCCGAAAGAGTTTCTGGTGTTGAGTTAGGTGCTGCACGGACTAAC
AGAGAAGGAAGAGATATCAGAGAACTTATGGAACAAacttgggatttggagcaccacccaacca
aatgggttagctgtccctgaagcaagcatgcattctgtaaaatcaaccacagatgttctggaat
tgatgaat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 583
Transcript exons: 42805727-42805891,42805973-42806226,42806327-42806490
ACTTGGGATTTGGAGCACCACCCAACCAAATGGGTTAGCTGTCCCTGAAGCAAGCATGCATTCT
GTAAAATCAACCACAGATGTTCTGGAATTGATGAATATCGGTTTAATGAATAGGGCTGTTGGTG
CTACCGCCCTAAATGAGAGGAGTAGCAGATCCCACAGCATTCTCACCGTTCATGTTCGTGGCAC
GGACATAAAAACTAATGCTGTTTTGCGCGGTAGTCTACATTTGGTGGATCTTGCTGGCAGTGAA
CGGGTAGATCGTTCTGAAGCAACTGGAGATAGGCTTAGGGAGGCACAACATATAAACAAATCCC
TGTCAGCTCTAGGAGATGTAATTTTTGCTCTGGCACAAAAGAATTCTCATGTCCCTTATAGAAA
CAGCAAACTGACTCAAGTTCTCCAGAGTTCTCTGGGTGGCCAGGCGAAAACCCTTATGTTTGTG
CAGCTTAATCCTGATGTAGAATCATACGCTGAAACCATAAGTACTTTGAAGTTTGCCGAAAGAG
TTTCTGGTGTTGAGTTAGGTGCTGCACGGACTAACAGAGAAGGAAGAGATATCAGAGAACTTAT
GGAACAA
						
						Genomic sequence
						
							ACTTGGGATTTGGAGCACCACCCAACCAAATGGGTTAGCTGTCCCTGAAGCAAGCATGCATTCT
GTAAAATCAACCACAGATGTTCTGGAATTGATGAATATCGGTTTAATGAATAGGGCTGTTGGTG
CTACCGCCCTAAATGAGAGGAGTAGCAGATCCCACAGGTTATATTTTACTCTGTATGTAACTTG
TTATTTGGAGAATTTGAGTTTTGCATCTAATGGGTTTCAGTAAATTATGTGCAGCATTCTCACC
GTTCATGTTCGTGGCACGGACATAAAAACTAATGCTGTTTTGCGCGGTAGTCTACATTTGGTGG
ATCTTGCTGGCAGTGAACGGGTAGATCGTTCTGAAGCAACTGGAGATAGGCTTAGGGAGGCACA
ACATATAAACAAATCCCTGTCAGCTCTAGGAGATGTAATTTTTGCTCTGGCACAAAAGAATTCT
CATGTCCCTTATAGAAACAGCAAACTGACTCAAGTTCTCCAGAGTTCTCTGGGTACTTTTTACT
GTTCAGATTTTAATTTGCTATTTCACGGTCATGCCCATTAGATTCTACATACCTTAAAAGCCTA
TTTATTTGCACTTACTGATTGTAGGTGGCCAGGCGAAAACCCTTATGTTTGTGCAGCTTAATCC
TGATGTAGAATCATACGCTGAAACCATAAGTACTTTGAAGTTTGCCGAAAGAGTTTCTGGTGTT
GAGTTAGGTGCTGCACGGACTAACAGAGAAGGAAGAGATATCAGAGAACTTATGGAACAA

Sequences
Splice junction sequence	CATAAGTACTTTGAAGTTTGCCGAAAGAGTTTCTGGTGTTGAGTTAGGTGCTGCACGGACTAAC AGAGAAGGAAGAGATATCAGAGAACTTATGGAACAAacttgggatttggagcaccacccaacca aatgggttagctgtccctgaagcaagcatgcattctgtaaaatcaaccacagatgttctggaat tgatgaat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 583 Transcript exons: 42805727-42805891,42805973-42806226,42806327-42806490 ACTTGGGATTTGGAGCACCACCCAACCAAATGGGTTAGCTGTCCCTGAAGCAAGCATGCATTCT GTAAAATCAACCACAGATGTTCTGGAATTGATGAATATCGGTTTAATGAATAGGGCTGTTGGTG CTACCGCCCTAAATGAGAGGAGTAGCAGATCCCACAGCATTCTCACCGTTCATGTTCGTGGCAC GGACATAAAAACTAATGCTGTTTTGCGCGGTAGTCTACATTTGGTGGATCTTGCTGGCAGTGAA CGGGTAGATCGTTCTGAAGCAACTGGAGATAGGCTTAGGGAGGCACAACATATAAACAAATCCC TGTCAGCTCTAGGAGATGTAATTTTTGCTCTGGCACAAAAGAATTCTCATGTCCCTTATAGAAA CAGCAAACTGACTCAAGTTCTCCAGAGTTCTCTGGGTGGCCAGGCGAAAACCCTTATGTTTGTG CAGCTTAATCCTGATGTAGAATCATACGCTGAAACCATAAGTACTTTGAAGTTTGCCGAAAGAG TTTCTGGTGTTGAGTTAGGTGCTGCACGGACTAACAGAGAAGGAAGAGATATCAGAGAACTTAT GGAACAA
Genomic sequence	ACTTGGGATTTGGAGCACCACCCAACCAAATGGGTTAGCTGTCCCTGAAGCAAGCATGCATTCT GTAAAATCAACCACAGATGTTCTGGAATTGATGAATATCGGTTTAATGAATAGGGCTGTTGGTG CTACCGCCCTAAATGAGAGGAGTAGCAGATCCCACAGGTTATATTTTACTCTGTATGTAACTTG TTATTTGGAGAATTTGAGTTTTGCATCTAATGGGTTTCAGTAAATTATGTGCAGCATTCTCACC GTTCATGTTCGTGGCACGGACATAAAAACTAATGCTGTTTTGCGCGGTAGTCTACATTTGGTGG ATCTTGCTGGCAGTGAACGGGTAGATCGTTCTGAAGCAACTGGAGATAGGCTTAGGGAGGCACA ACATATAAACAAATCCCTGTCAGCTCTAGGAGATGTAATTTTTGCTCTGGCACAAAAGAATTCT CATGTCCCTTATAGAAACAGCAAACTGACTCAAGTTCTCCAGAGTTCTCTGGGTACTTTTTACT GTTCAGATTTTAATTTGCTATTTCACGGTCATGCCCATTAGATTCTACATACCTTAAAAGCCTA TTTATTTGCACTTACTGATTGTAGGTGGCCAGGCGAAAACCCTTATGTTTGTGCAGCTTAATCC TGATGTAGAATCATACGCTGAAACCATAAGTACTTTGAAGTTTGCCGAAAGAGTTTCTGGTGTT GAGTTAGGTGCTGCACGGACTAACAGAGAAGGAAGAGATATCAGAGAACTTATGGAACAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.704858082

Conservation Information
Conserved circRNAs	NA
PMCS	0.704858082

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							42805782-42805771(+)
42805976-42806436(-)
						
						Potential amino acid sequence
						
							MHSVKSTTDVLELMNIGLMNRAVGATALNERSSRSHSILTVHVRGTDIKTNAVLRGSLHLVDLA
GSERVDRSEATGDRLREAQHINKSLSALGDVIFALAQKNSHVPYRNSKLTQVLQSSLGGQAKTL
MFVQLNPDVESYAETISTLKFAERVSGVELGAARTNREGRDIRELMEQTWDLEHHPTKWVSCP*
(+)
MLWDLLLLSFRAVAPTALFIKPIFINSRTSVVDFTECMLASGTANPFGWVVLQIPSLFHKFSDI
SSFSVSPCST*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	42805782-42805771(+) 42805976-42806436(-)
Potential amino acid sequence	MHSVKSTTDVLELMNIGLMNRAVGATALNERSSRSHSILTVHVRGTDIKTNAVLRGSLHLVDLA GSERVDRSEATGDRLREAQHINKSLSALGDVIFALAQKNSHVPYRNSKLTQVLQSSLGGQAKTL MFVQLNPDVESYAETISTLKFAERVSGVELGAARTNREGRDIRELMEQTWDLEHHPTKWVSCP* (+) MLWDLLLLSFRAVAPTALFIKPIFINSRTSVVDFTECMLASGTANPFGWVVLQIPSLFHKFSDI SSFSVSPCST*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.003G158800_circ_g.1

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