Plant Circular RNA Database

Detail information of Zm00001d002844_circ_g.1

			
						 General Information
					
						CircRNA Name
						Zm00001d002844_circ_g.1
					
						ID in PlantcircBase
						zma_circ_003866
					
						Alias
						pollen_test_circ_000794
					
						Organism
						
							Zea mays						
					
						Position
						
							chr2: 24434069-24434867  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d002844						
					
						Parent gene annotation
						
							LIM domain-containing protein PLIM2b
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d002844_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						pollen
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d002844_T007:3
Zm00001d002844_T001:1
Zm00001d002844_T004:1
Zm00001d002844_T005:3
Zm00001d002844_T002:4
Zm00001d002844_T003:4
Zm00001d002844_T008:2
Zm00001d002844_T006:3
Zm00001d002844_T009:2

General Information
CircRNA Name	Zm00001d002844_circ_g.1
ID in PlantcircBase	zma_circ_003866
Alias	pollen_test_circ_000794
Organism	Zea mays
Position	chr2: 24434069-24434867 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	Zm00001d002844
Parent gene annotation	LIM domain-containing protein PLIM2b
Parent gene strand	+
Alternative splicing	Zm00001d002844_circ_g.2
Support reads	NA
Tissues	pollen
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d002844_T007:3 Zm00001d002844_T001:1 Zm00001d002844_T004:1 Zm00001d002844_T005:3 Zm00001d002844_T002:4 Zm00001d002844_T003:4 Zm00001d002844_T008:2 Zm00001d002844_T006:3 Zm00001d002844_T009:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CACTTTGCAGACAAGGGCCCCAAGCAAGCTGTCGTCTGCATTTTCTGGTACCCAGGATAAGTGC
GCAGCATGCCAGAAAACAGTGTACCCGCTGGAGAAGgagaagcgatcgggattgtgggcgagtg
cgtgcgttcgtgcagccacagacaggcgtccgggcaccatgtctttcaccggcacgcaggacaa
gtgcacgg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GAGAAGCGATCGGGATTGTGGGCGAGTGCGTGCGTTCGTGCAGCCACAGACAGGCGTCCGGGCA
CCATGTCTTTCACCGGCACGCAGGACAAGTGCACGGCGTGCGACAAGACCGTCCACTTCATCGA
CCTCCTCACGGCCGACGGCGCCATCTACCATAAGACATGCTTCAAGTGCAGCCACTGCAAAGGG
GTCCTCTCGGTACGTGCATATGTCCTGATGTCCATGGCTTAGAAAAACGATCGATCCAGTTTAT
TATCATCCGATCTCTGCATGCAAAAACACAAAAACAAGAGCTTTCTGACGACGATGCAGCAAAC
CGAGAATGATGCGTTAATTGGCCGCCTGCAGATGTGCAGCTACTCCTCCATGGACGGTGTGCTG
TACTGCAAGACCCACTTCGAGCAGCTCTTCAAGGAGACCGGGAGCTTCTCCAAGAACTTCACGC
CAGGTAATGTAACAGTTGCTGCGTGCTACTAGATCTTCAGTTGCCACGAACTTAATTTGTCGCG
CCGAAAAAAAAAGGAATTTATCCTGAAAAACTTTCTTGTGTATGTAATTCAGGTGGCAAGTCAT
CAGACAAGGGTGAACTGGTACAGCTAGAGCCTCACAAAACTAGCACCATCTTCTGGAATTCCTT
TCTTTATTTGTAGTAATAACTGGATAAATGTCTGCCAAACAAATTGTTGGAACCCTGTGCACTT
TGCAGACAAGGGCCCCAAGCAAGCTGTCGTCTGCATTTTCTGGTACCCAGGATAAGTGCGCAGC
ATGCCAGAAAACAGTGTACCCGCTGGAGAAG

Sequences
Splice junction sequence	CACTTTGCAGACAAGGGCCCCAAGCAAGCTGTCGTCTGCATTTTCTGGTACCCAGGATAAGTGC GCAGCATGCCAGAAAACAGTGTACCCGCTGGAGAAGgagaagcgatcgggattgtgggcgagtg cgtgcgttcgtgcagccacagacaggcgtccgggcaccatgtctttcaccggcacgcaggacaa gtgcacgg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GAGAAGCGATCGGGATTGTGGGCGAGTGCGTGCGTTCGTGCAGCCACAGACAGGCGTCCGGGCA CCATGTCTTTCACCGGCACGCAGGACAAGTGCACGGCGTGCGACAAGACCGTCCACTTCATCGA CCTCCTCACGGCCGACGGCGCCATCTACCATAAGACATGCTTCAAGTGCAGCCACTGCAAAGGG GTCCTCTCGGTACGTGCATATGTCCTGATGTCCATGGCTTAGAAAAACGATCGATCCAGTTTAT TATCATCCGATCTCTGCATGCAAAAACACAAAAACAAGAGCTTTCTGACGACGATGCAGCAAAC CGAGAATGATGCGTTAATTGGCCGCCTGCAGATGTGCAGCTACTCCTCCATGGACGGTGTGCTG TACTGCAAGACCCACTTCGAGCAGCTCTTCAAGGAGACCGGGAGCTTCTCCAAGAACTTCACGC CAGGTAATGTAACAGTTGCTGCGTGCTACTAGATCTTCAGTTGCCACGAACTTAATTTGTCGCG CCGAAAAAAAAAGGAATTTATCCTGAAAAACTTTCTTGTGTATGTAATTCAGGTGGCAAGTCAT CAGACAAGGGTGAACTGGTACAGCTAGAGCCTCACAAAACTAGCACCATCTTCTGGAATTCCTT TCTTTATTTGTAGTAATAACTGGATAAATGTCTGCCAAACAAATTGTTGGAACCCTGTGCACTT TGCAGACAAGGGCCCCAAGCAAGCTGTCGTCTGCATTTTCTGGTACCCAGGATAAGTGCGCAGC ATGCCAGAAAACAGTGTACCCGCTGGAGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.029620359

Conservation Information
Conserved circRNAs	NA
PMCS	0.029620359

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24434136-24434864(+)
24434838-24434226(+)
24434229-24434646(-)
24434222-24434430(-)
						
						Potential amino acid sequence
						
							MSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHCKGVLSMCSYSSMDGVLYCKTHFEQ
LFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQDKCAACQKTVYPLEKEKRSGLWASA
CVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHCKGVLSMCSYSSM
DGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQDKCAACQKTVYPL
EKEKRSGLWASACVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHC
KGVLSMCSYSSMDGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQD
KCAACQKTVYPLEKEKRSGLWASACVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGA
IYHKTCFKCSHCKGVLSMCSYSSMDGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAP
SKLSSAFSGTQDKCAACQKTVYPLEK(+)
MPENSVPAGEGEAIGIVGECVRSCSHRQASGHHVFHRHAGQVHGVRQDRPLHRPPHGRRRHLP*
(+)
MVDGAVGREEVDEVDGLVARRALVLRAGERHGARTPVCGCTNARTRPQSRSLLLLQRVHCFLAC
CALILGTRKCRRQLAWGPCQFTLV*(-)
MAPSAVRRSMKWTVLSHAVHLSCVPVKDMVPGRLSVAARTHALAHNPDRFSFSSGYTVFWHAAH
LSWVPENADDSLLGALVSSPLSDDLPPGVKFLEKLPVSLKSCSKWVLQYSTPSMEE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24434136-24434864(+) 24434838-24434226(+) 24434229-24434646(-) 24434222-24434430(-)
Potential amino acid sequence	MSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHCKGVLSMCSYSSMDGVLYCKTHFEQ LFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQDKCAACQKTVYPLEKEKRSGLWASA CVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHCKGVLSMCSYSSM DGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQDKCAACQKTVYPL EKEKRSGLWASACVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGAIYHKTCFKCSHC KGVLSMCSYSSMDGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAPSKLSSAFSGTQD KCAACQKTVYPLEKEKRSGLWASACVRAATDRRPGTMSFTGTQDKCTACDKTVHFIDLLTADGA IYHKTCFKCSHCKGVLSMCSYSSMDGVLYCKTHFEQLFKETGSFSKNFTPGGKSSDKGELTRAP SKLSSAFSGTQDKCAACQKTVYPLEK(+) MPENSVPAGEGEAIGIVGECVRSCSHRQASGHHVFHRHAGQVHGVRQDRPLHRPPHGRRRHLP* (+) MVDGAVGREEVDEVDGLVARRALVLRAGERHGARTPVCGCTNARTRPQSRSLLLLQRVHCFLAC CALILGTRKCRRQLAWGPCQFTLV(-) MAPSAVRRSMKWTVLSHAVHLSCVPVKDMVPGRLSVAARTHALAHNPDRFSFSSGYTVFWHAAH LSWVPENADDSLLGALVSSPLSDDLPPGVKFLEKLPVSLKSCSKWVLQYSTPSMEE(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Tang et al., 2018

circRNA

Detail information of Zm00001d002844_circ_g.1

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