Plant Circular RNA Database

Detail information of orai.009G208800_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.009G208800_circ_g.2
					
						ID in PlantcircBase
						gra_circ_000956
					
						Alias
						Chr09:16194546|16195236
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr09: 16194546-16195236  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.009G208800						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.009G208800_circ_g.1 						
					
						Support reads
						14
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.009G208800.3:3
Gorai.009G208800.5:3
Gorai.009G208800.4:3
Gorai.009G208800.2:3
Gorai.009G208800.1:3

General Information
CircRNA Name	orai.009G208800_circ_g.2
ID in PlantcircBase	gra_circ_000956
Alias	Chr09:16194546\|16195236
Organism	Gossypium raimondii
Position	chrChr09: 16194546-16195236 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.009G208800
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.009G208800_circ_g.1
Support reads	14
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.009G208800.3:3 Gorai.009G208800.5:3 Gorai.009G208800.4:3 Gorai.009G208800.2:3 Gorai.009G208800.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCACTTGAATGCTTGTTCGTATAGGGACATAAAAGGTGCAAATTTGCTGGTCGATGCCAACGGG
ATTGTTAAACTTGCTGATTTTGGCATGGCAAAACATgaaattagagttttgagacgcttaaagc
accaaaatattgttcaatactatggcagtgaaatagtatgcagtccttccacttctacactatg
ctgaaata

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GAAATTAGAGTTTTGAGACGCTTAAAGCACCAAAATATTGTTCAATACTATGGCAGTGAAATAG
TATGCAGTCCTTCCACTTCTACACTATGCTGAAATAGTTACTTTTAAGCTGGGTTAATTTGAGG
GGTTCATTGTTTCATATATAGGTTGATGATCGCTTATACATATATTTGGAGTATGTCCATCCAG
GCTCAATTAATAAATATGTCCGTGAGCACTGTGGAGCTATAACAGAATCAATAGTTCGCAATTT
CACCCGTCATATTCTATCTGGCTTAGCATACCTTCATAGTTTCAAGACAATACACAGGTGACCT
TCATGCTCTGTTAGAATTATATAGCAGCTTAACTACTTGTTTATTTTGTATTTAGTGCGTTCAA
AATTGATAAATCAAGGGCGAACAATCCTTTGTAAAATATATAAATTGCAAAATCCTAATGCACC
AATGGTTCTTTTCGAACTTTTGTTGTAAGGGAACTTCTGCTCCATGTTACAGCCATCGAAATGG
TTGAATTAAAAATTTCTTCTAGATGTGATTCTGTTTTTTGTTATAGTATTTGATGTATCTTGTC
AACATCGGATTTCATTCACTTGAATGCTTGTTCGTATAGGGACATAAAAGGTGCAAATTTGCTG
GTCGATGCCAACGGGATTGTTAAACTTGCTGATTTTGGCATGGCAAAACAT

Sequences
Splice junction sequence	TCACTTGAATGCTTGTTCGTATAGGGACATAAAAGGTGCAAATTTGCTGGTCGATGCCAACGGG ATTGTTAAACTTGCTGATTTTGGCATGGCAAAACATgaaattagagttttgagacgcttaaagc accaaaatattgttcaatactatggcagtgaaatagtatgcagtccttccacttctacactatg ctgaaata
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GAAATTAGAGTTTTGAGACGCTTAAAGCACCAAAATATTGTTCAATACTATGGCAGTGAAATAG TATGCAGTCCTTCCACTTCTACACTATGCTGAAATAGTTACTTTTAAGCTGGGTTAATTTGAGG GGTTCATTGTTTCATATATAGGTTGATGATCGCTTATACATATATTTGGAGTATGTCCATCCAG GCTCAATTAATAAATATGTCCGTGAGCACTGTGGAGCTATAACAGAATCAATAGTTCGCAATTT CACCCGTCATATTCTATCTGGCTTAGCATACCTTCATAGTTTCAAGACAATACACAGGTGACCT TCATGCTCTGTTAGAATTATATAGCAGCTTAACTACTTGTTTATTTTGTATTTAGTGCGTTCAA AATTGATAAATCAAGGGCGAACAATCCTTTGTAAAATATATAAATTGCAAAATCCTAATGCACC AATGGTTCTTTTCGAACTTTTGTTGTAAGGGAACTTCTGCTCCATGTTACAGCCATCGAAATGG TTGAATTAAAAATTTCTTCTAGATGTGATTCTGTTTTTTGTTATAGTATTTGATGTATCTTGTC AACATCGGATTTCATTCACTTGAATGCTTGTTCGTATAGGGACATAAAAGGTGCAAATTTGCTG GTCGATGCCAACGGGATTGTTAAACTTGCTGATTTTGGCATGGCAAAACAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.132176628

Conservation Information
Conserved circRNAs	NA
PMCS	0.132176628

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							16195225-16195233(+)
16195190-16194558(+)
						
						Potential amino acid sequence
						
							MAKHEIRVLRRLKHQNIVQYYGSEIVDDRLYIYLEYVHPGSINKYVREHCGAITESIVRNFTRH
ILSGLAYLHSFKTIHRDIKGANLLVDANGIVKLADFGMAKHEIRVLRRLKHQNIVQYYGSEIVD
DRLYIYLEYVHPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSFKTIHRDIKGANLLVDA
NGIVKLADFGMAKHEIRVLRRLKHQNIVQYYGSEIVDDRLYIYLEYVHPGSINKYVREHCGAIT
ESIVRNFTRHILSGLAYLHSFKTIHRDIKGANLLVDANGIVKLADFGMAKH(+)
MPTGLLNLLILAWQNMKLEF*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	16195225-16195233(+) 16195190-16194558(+)
Potential amino acid sequence	MAKHEIRVLRRLKHQNIVQYYGSEIVDDRLYIYLEYVHPGSINKYVREHCGAITESIVRNFTRH ILSGLAYLHSFKTIHRDIKGANLLVDANGIVKLADFGMAKHEIRVLRRLKHQNIVQYYGSEIVD DRLYIYLEYVHPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSFKTIHRDIKGANLLVDA NGIVKLADFGMAKHEIRVLRRLKHQNIVQYYGSEIVDDRLYIYLEYVHPGSINKYVREHCGAIT ESIVRNFTRHILSGLAYLHSFKTIHRDIKGANLLVDANGIVKLADFGMAKH(+) MPTGLLNLLILAWQNMKLEF*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.009G208800_circ_g.2

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