Plant Circular RNA Database

Detail information of AT4G25520_circ_g.1

			
						 General Information
					
						CircRNA Name
						AT4G25520_circ_g.1
					
						ID in PlantcircBase
						ath_circ_032772
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 13033674-13034815  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT4G25520						
					
						Parent gene annotation
						
							Probable transcriptional regulator SLK1
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT4G25520_circ_g.2 						
					
						Support reads
						1
					
						Tissues
						whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G25520.2:5
AT4G25520.1:5

General Information
CircRNA Name	AT4G25520_circ_g.1
ID in PlantcircBase	ath_circ_032772
Alias	NA
Organism	Arabidpsis thaliana
Position	chr4: 13033674-13034815 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT4G25520
Parent gene annotation	Probable transcriptional regulator SLK1
Parent gene strand	-
Alternative splicing	AT4G25520_circ_g.2
Support reads	1
Tissues	whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G25520.2:5 AT4G25520.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAGGCACAGATATATAAGTACACACAAGAATGAAATTTGCAACTAACTTACCAAATCCCTTTCC
AGACTTGGTGCCGCAAAGATCACACTGCCACATATCctgcagagtcctgatatatctttttgga
tagccaagatcattcagcgactgtagttccatgaactttgccagttgccgtcctgctcccaaga
ccctgata

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 528
Transcript exons: 13033674-13033767,13033860-13033960,13034222-13034290,13
034392-13034606,13034767-13034815
CTGCAGAGTCCTGATATATCTTTTTGGATAGCCAAGATCATTCAGCGACTGTAGTTCCATGAAC
TTTGCCAGTTGCCGTCCTGCTCCCAAGACCATGTTACTATTTGACTGTAAATCCTGCTGAGAAA
CCCCCTCTGACCCACTCTCTGAGATCGTGCTCTGGCATTTCTGTGCAACCTGAAGCAATTGGTT
CACCTGTGGAGCAATAAGTCTGCGAAGAAGAAGCTCTTCATGACGCCGAGCGCAAAACTCCCAA
GAAAGTATCTTCAAATCTTGAGAAAATATGATGCGAAGATGGCCCTCACGGACAACACGAAACT
GCTCGTGTACAGTTTCCTGAACTGCTTTTCTATATTCTAACATCATCAGTCCATTGGGAAATCT
GTTTTCTCGAGGGTGATCCAGATATAACAGCTCATCAATAATACCACTTGCAAATTTGATTTCA
ATTAGTCTGGCAAGCACATCAAACGTTGCCTCAAATCCCTTTCCAGACTTGGTGCCGCAAAGAT
CACACTGCCACATATC
						
					

						Genomic sequence
						
							CTGCAGAGTCCTGATATATCTTTTTGGATAGCCAAGATCATTCAGCGACTGTAGTTCCATGAAC
TTTGCCAGTTGCCGTCCTGCTCCCAAGACCCTGATACACAAAATGGCACCCAGCAATGATAAAA
AGCACTAGAAACAAATGCCAGAGTATCGACAATAAAAGAATAATGTGCATTAACTTACATGTTA
CTATTTGACTGTAAATCCTGCTGAGAAACCCCCTCTGACCCACTCTCTGAGATCGTGCTCTGGC
ATTTCTGTGCAACCTGAAGCAATTGGTTCACCTGCATGACCAATAAATAAGATTTTAGTACACT
TCTTAGCACCGTTGCAGCATCTGTAAACATCTGTTCTGAAGTAAAATAACAGACTTTGTGCTCT
ATTCATAGGAAACCAGAAGATAAGCTACACAGAAACCGGGTATTAGCTATCCTCAATTTCATAA
CAAGGGTTTAAGGAAACAAAGTCATACAGTTGACCACAAACATTTTATGTAAAGTCTTAAGTTC
CAATTAATAAACGCAAGGGTCGTGCCATTAGAATACCTGTGGAGCAATAAGTCTGCGAAGAAGA
AGCTCTTCATGACGCCGAGCGCAAAACTCCCAAGAAAGTATCTAGATAGAAGCAAAGTCAAGAT
TAACTAAGCATTAGCATACTGGTGCCTGATTTTTTCATCGCTTTACACAATTTGGAACCAGAGA
ATTGTTCTAGATACCTTCAAATCTTGAGAAAATATGATGCGAAGATGGCCCTCACGGACAACAC
GAAACTGCTCGTGTACAGTTTCCTGAACTGCTTTTCTATATTCTAACATCATCAGTCCATTGGG
AAATCTGTTTTCTCGAGGGTGATCCAGATATAACAGCTCATCAATAATACCACTTGCAAATTTG
ATTTCAATTAGTCTGGCAAGCACATCAAACGTTGCCTCTGCGGATTAACACGAGAAGTGACAGT
TAGCTACTATTTTGACTTAATAGTTATAAACAATAAAAAGAGACCAATTAGCATGTGTAAGTTG
CTTCATTGTGCTCAAGTGAAGGCACAGATATATAAGTACACACAAGAATGAAATTTGCAACTAA
CTTACCAAATCCCTTTCCAGACTTGGTGCCGCAAAGATCACACTGCCACATATC
						
					

			
		

Sequences
Splice junction sequence	AAGGCACAGATATATAAGTACACACAAGAATGAAATTTGCAACTAACTTACCAAATCCCTTTCC AGACTTGGTGCCGCAAAGATCACACTGCCACATATCctgcagagtcctgatatatctttttgga tagccaagatcattcagcgactgtagttccatgaactttgccagttgccgtcctgctcccaaga ccctgata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 528 Transcript exons: 13033674-13033767,13033860-13033960,13034222-13034290,13 034392-13034606,13034767-13034815 CTGCAGAGTCCTGATATATCTTTTTGGATAGCCAAGATCATTCAGCGACTGTAGTTCCATGAAC TTTGCCAGTTGCCGTCCTGCTCCCAAGACCATGTTACTATTTGACTGTAAATCCTGCTGAGAAA CCCCCTCTGACCCACTCTCTGAGATCGTGCTCTGGCATTTCTGTGCAACCTGAAGCAATTGGTT CACCTGTGGAGCAATAAGTCTGCGAAGAAGAAGCTCTTCATGACGCCGAGCGCAAAACTCCCAA GAAAGTATCTTCAAATCTTGAGAAAATATGATGCGAAGATGGCCCTCACGGACAACACGAAACT GCTCGTGTACAGTTTCCTGAACTGCTTTTCTATATTCTAACATCATCAGTCCATTGGGAAATCT GTTTTCTCGAGGGTGATCCAGATATAACAGCTCATCAATAATACCACTTGCAAATTTGATTTCA ATTAGTCTGGCAAGCACATCAAACGTTGCCTCAAATCCCTTTCCAGACTTGGTGCCGCAAAGAT CACACTGCCACATATC
Genomic sequence	CTGCAGAGTCCTGATATATCTTTTTGGATAGCCAAGATCATTCAGCGACTGTAGTTCCATGAAC TTTGCCAGTTGCCGTCCTGCTCCCAAGACCCTGATACACAAAATGGCACCCAGCAATGATAAAA AGCACTAGAAACAAATGCCAGAGTATCGACAATAAAAGAATAATGTGCATTAACTTACATGTTA CTATTTGACTGTAAATCCTGCTGAGAAACCCCCTCTGACCCACTCTCTGAGATCGTGCTCTGGC ATTTCTGTGCAACCTGAAGCAATTGGTTCACCTGCATGACCAATAAATAAGATTTTAGTACACT TCTTAGCACCGTTGCAGCATCTGTAAACATCTGTTCTGAAGTAAAATAACAGACTTTGTGCTCT ATTCATAGGAAACCAGAAGATAAGCTACACAGAAACCGGGTATTAGCTATCCTCAATTTCATAA CAAGGGTTTAAGGAAACAAAGTCATACAGTTGACCACAAACATTTTATGTAAAGTCTTAAGTTC CAATTAATAAACGCAAGGGTCGTGCCATTAGAATACCTGTGGAGCAATAAGTCTGCGAAGAAGA AGCTCTTCATGACGCCGAGCGCAAAACTCCCAAGAAAGTATCTAGATAGAAGCAAAGTCAAGAT TAACTAAGCATTAGCATACTGGTGCCTGATTTTTTCATCGCTTTACACAATTTGGAACCAGAGA ATTGTTCTAGATACCTTCAAATCTTGAGAAAATATGATGCGAAGATGGCCCTCACGGACAACAC GAAACTGCTCGTGTACAGTTTCCTGAACTGCTTTTCTATATTCTAACATCATCAGTCCATTGGG AAATCTGTTTTCTCGAGGGTGATCCAGATATAACAGCTCATCAATAATACCACTTGCAAATTTG ATTTCAATTAGTCTGGCAAGCACATCAAACGTTGCCTCTGCGGATTAACACGAGAAGTGACAGT TAGCTACTATTTTGACTTAATAGTTATAAACAATAAAAAGAGACCAATTAGCATGTGTAAGTTG CTTCATTGTGCTCAAGTGAAGGCACAGATATATAAGTACACACAAGAATGAAATTTGCAACTAA CTTACCAAATCCCTTTCCAGACTTGGTGCCGCAAAGATCACACTGCCACATATC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.21101774

Conservation Information
Conserved circRNAs	NA
PMCS	0.21101774

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13034813-13033676(-)
						
						Potential amino acid sequence
						
							MWQCDLCGTKSGKGFEATFDVLARLIEIKFASGIIDELLYLDHPRENRFPNGLMMLEYRKAVQE
TVHEQFRVVREGHLRIIFSQDLKILSWEFCARRHEELLLRRLIAPQVNQLLQVAQKCQSTISES
GSEGVSQQDLQSNSNMVLGAGRQLAKFMELQSLNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFE
ATFDVLARLIEIKFASGIIDELLYLDHPRENRFPNGLMMLEYRKAVQETVHEQFRVVREGHLRI
IFSQDLKILSWEFCARRHEELLLRRLIAPQVNQLLQVAQKCQSTISESGSEGVSQQDLQSNSNM
VLGAGRQLAKFMELQSLNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFEATFDVLARLIEIKFAS
GIIDELLYLDHPRENRFPNGLMMLEYRKAVQETVHEQFRVVREGHLRIIFSQDLKILSWEFCAR
RHEELLLRRLIAPQVNQLLQVAQKCQSTISESGSEGVSQQDLQSNSNMVLGAGRQLAKFMELQS
LNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFEATFDVLARLIEIKFASGIIDELLYLDHPRENR
FPNGLMMLEYRKAVQETVHEQFRVVREGHLRIIFSQDLKILSWEFCARRHEELLLRRLIAPQVN
QLLQVAQKCQSTISESGSEGVSQQDLQSNSNMVLGAGRQLAKFMELQSLNDLGYPKRYIRTLQ(
-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13034813-13033676(-)
Potential amino acid sequence	MWQCDLCGTKSGKGFEATFDVLARLIEIKFASGIIDELLYLDHPRENRFPNGLMMLEYRKAVQE TVHEQFRVVREGHLRIIFSQDLKILSWEFCARRHEELLLRRLIAPQVNQLLQVAQKCQSTISES GSEGVSQQDLQSNSNMVLGAGRQLAKFMELQSLNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFE ATFDVLARLIEIKFASGIIDELLYLDHPRENRFPNGLMMLEYRKAVQETVHEQFRVVREGHLRI IFSQDLKILSWEFCARRHEELLLRRLIAPQVNQLLQVAQKCQSTISESGSEGVSQQDLQSNSNM VLGAGRQLAKFMELQSLNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFEATFDVLARLIEIKFAS GIIDELLYLDHPRENRFPNGLMMLEYRKAVQETVHEQFRVVREGHLRIIFSQDLKILSWEFCAR RHEELLLRRLIAPQVNQLLQVAQKCQSTISESGSEGVSQQDLQSNSNMVLGAGRQLAKFMELQS LNDLGYPKRYIRTLQDMWQCDLCGTKSGKGFEATFDVLARLIEIKFASGIIDELLYLDHPRENR FPNGLMMLEYRKAVQETVHEQFRVVREGHLRIIFSQDLKILSWEFCARRHEELLLRRLIAPQVN QLLQVAQKCQSTISESGSEGVSQQDLQSNSNMVLGAGRQLAKFMELQSLNDLGYPKRYIRTLQ( -)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT4G25520_circ_g.1

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