Plant Circular RNA Database

Detail information of AT5G04140_circ_g.36

			
						 General Information
					
						CircRNA Name
						AT5G04140_circ_g.36
					
						ID in PlantcircBase
						ath_circ_036338
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 1135700-1136294  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT5G04140						
					
						Parent gene annotation
						
							glutamate synthase 1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G04140_circ_g.3 AT5G04140_circ_g.4 AT5G04140_circ_g.5 AT5G04140_circ_g.6 AT5G04140_circ_g.7 AT5G04140_circ_g.8 AT5G04140_circ_g.9 AT5G04140_circ_g.10 AT5G04140_circ_g.11 AT5G04140_circ_g.12 AT5G04140_circ_g.13 AT5G04140_circ_g.14 AT5G04140_circ_g.15 AT5G04140_circ_g.16 AT5G04140_circ_g.17 AT5G04140_circ_g.18 AT5G04140_circ_g.19 AT5G04140_circ_g.20 AT5G04140_circ_g.21 AT5G04140_circ_g.22 AT5G04140_circ_g.23 AT5G04140_circ_g.24 AT5G04140_circ_g.25 AT5G04140_circ_g.26 AT5G04140_circ_g.27 AT5G04140_circ_g.28 AT5G04140_circ_g.29 AT5G04140_circ_g.30 AT5G04140_circ_g.31 AT5G04140_circ_g.32 AT5G04140_circ_g.33 AT5G04140_circ_g.34 AT5G04140_circ_g.35 AT5G04140_circ_g.37 AT5G04140_circ_g.38 AT5G04140_circ_g.39 AT5G04140_circ_g.40 AT5G04140_circ_g.41 AT5G04140_circ_g.42 AT5G04140_circ_g.43 AT5G04140_circ_g.44 AT5G04140_circ_g.45 AT5G04140_circ_g.46 						
					
						Support reads
						2
					
						Tissues
						seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G04140.2:3
AT5G04140.1:3

General Information
CircRNA Name	AT5G04140_circ_g.36
ID in PlantcircBase	ath_circ_036338
Alias	NA
Organism	Arabidpsis thaliana
Position	chr5: 1135700-1136294 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	AT5G04140
Parent gene annotation	glutamate synthase 1
Parent gene strand	+
Alternative splicing	AT5G04140_circ_g.3 AT5G04140_circ_g.4 AT5G04140_circ_g.5 AT5G04140_circ_g.6 AT5G04140_circ_g.7 AT5G04140_circ_g.8 AT5G04140_circ_g.9 AT5G04140_circ_g.10 AT5G04140_circ_g.11 AT5G04140_circ_g.12 AT5G04140_circ_g.13 AT5G04140_circ_g.14 AT5G04140_circ_g.15 AT5G04140_circ_g.16 AT5G04140_circ_g.17 AT5G04140_circ_g.18 AT5G04140_circ_g.19 AT5G04140_circ_g.20 AT5G04140_circ_g.21 AT5G04140_circ_g.22 AT5G04140_circ_g.23 AT5G04140_circ_g.24 AT5G04140_circ_g.25 AT5G04140_circ_g.26 AT5G04140_circ_g.27 AT5G04140_circ_g.28 AT5G04140_circ_g.29 AT5G04140_circ_g.30 AT5G04140_circ_g.31 AT5G04140_circ_g.32 AT5G04140_circ_g.33 AT5G04140_circ_g.34 AT5G04140_circ_g.35 AT5G04140_circ_g.37 AT5G04140_circ_g.38 AT5G04140_circ_g.39 AT5G04140_circ_g.40 AT5G04140_circ_g.41 AT5G04140_circ_g.42 AT5G04140_circ_g.43 AT5G04140_circ_g.44 AT5G04140_circ_g.45 AT5G04140_circ_g.46
Support reads	2
Tissues	seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G04140.2:3 AT5G04140.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGATGAATACGGATTCGGTTCCTTGGCAATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGC
CACACTAATAATTGCCCAGTGGGTGTAGCAAGTCAGattaatccaaatgcaaaagtatcagtca
agctagttgcagaagctggaatcggaactgttgcttcaggagttgcaaagggtaacgctgatat
catccagg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 393
Transcript exons: 1135700-1135798,1135913-1136008,1136097-1136294
ATTAATCCAAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTT
CAGGAGTTGCAAAGGGTAACGCTGATATCATCCAGATATCAGGCCATGATGGTGGAACCGGGGC
TAGTCCAATAAGCTCCATAAAACATGCTGGTGGACCATGGGAACTTGGACTAACAGAAACTCAC
CAAACACTTATCGCAAATGGACTAAGAGAAAGAGTCATTTTAAGAGTCGATGGAGGCTTAAAGA
GTGGTGTTGATGTTCTAATGGCTGCAGCTATGGGTGCTGATGAATACGGATTCGGTTCCTTGGC
AATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGCCACACTAATAATTGCCCAGTGGGTGTA
GCAAGTCAG
						
						Genomic sequence
						
							ATTAATCCAAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTT
CAGGAGTTGCAAAGGGTAACGCTGATATCATCCAGGTAACTTGAAAGTTGAAAATATAACATTG
CATCTTGTTACTCCCATGTTCTTGTGCCGTCTCATTTTGTGTCTGCCAAGAAGTTTCATTTTGT
TCGTTTTGATATGTTTTTCAGATATCAGGCCATGATGGTGGAACCGGGGCTAGTCCAATAAGCT
CCATAAAACATGCTGGTGGACCATGGGAACTTGGACTAACAGAAACTCACCAAGTAAAAACCTT
TTGGCTACCATGTGGTACTAATTCTCTTGGACGTTCTGAGTCTCTAAGCAAAAAAACTTATTTC
TTCTCTTTTGCAGACACTTATCGCAAATGGACTAAGAGAAAGAGTCATTTTAAGAGTCGATGGA
GGCTTAAAGAGTGGTGTTGATGTTCTAATGGCTGCAGCTATGGGTGCTGATGAATACGGATTCG
GTTCCTTGGCAATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGCCACACTAATAATTGCCC
AGTGGGTGTAGCAAGTCAG

Sequences
Splice junction sequence	TGATGAATACGGATTCGGTTCCTTGGCAATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGC CACACTAATAATTGCCCAGTGGGTGTAGCAAGTCAGattaatccaaatgcaaaagtatcagtca agctagttgcagaagctggaatcggaactgttgcttcaggagttgcaaagggtaacgctgatat catccagg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 393 Transcript exons: 1135700-1135798,1135913-1136008,1136097-1136294 ATTAATCCAAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTT CAGGAGTTGCAAAGGGTAACGCTGATATCATCCAGATATCAGGCCATGATGGTGGAACCGGGGC TAGTCCAATAAGCTCCATAAAACATGCTGGTGGACCATGGGAACTTGGACTAACAGAAACTCAC CAAACACTTATCGCAAATGGACTAAGAGAAAGAGTCATTTTAAGAGTCGATGGAGGCTTAAAGA GTGGTGTTGATGTTCTAATGGCTGCAGCTATGGGTGCTGATGAATACGGATTCGGTTCCTTGGC AATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGCCACACTAATAATTGCCCAGTGGGTGTA GCAAGTCAG
Genomic sequence	ATTAATCCAAATGCAAAAGTATCAGTCAAGCTAGTTGCAGAAGCTGGAATCGGAACTGTTGCTT CAGGAGTTGCAAAGGGTAACGCTGATATCATCCAGGTAACTTGAAAGTTGAAAATATAACATTG CATCTTGTTACTCCCATGTTCTTGTGCCGTCTCATTTTGTGTCTGCCAAGAAGTTTCATTTTGT TCGTTTTGATATGTTTTTCAGATATCAGGCCATGATGGTGGAACCGGGGCTAGTCCAATAAGCT CCATAAAACATGCTGGTGGACCATGGGAACTTGGACTAACAGAAACTCACCAAGTAAAAACCTT TTGGCTACCATGTGGTACTAATTCTCTTGGACGTTCTGAGTCTCTAAGCAAAAAAACTTATTTC TTCTCTTTTGCAGACACTTATCGCAAATGGACTAAGAGAAAGAGTCATTTTAAGAGTCGATGGA GGCTTAAAGAGTGGTGTTGATGTTCTAATGGCTGCAGCTATGGGTGCTGATGAATACGGATTCG GTTCCTTGGCAATGATTGCTACTGGTTGTGTTATGGCTCGTATTTGCCACACTAATAATTGCCC AGTGGGTGTAGCAAGTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.277475445

Conservation Information
Conserved circRNAs	NA
PMCS	0.277475445

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1136175-1136291(+)
						
						Potential amino acid sequence
						
							MAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIGTVASGV
AKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLKSGV
DVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIGTVA
SGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLK
SGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIG
TVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDG
GLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQ(+)
						
						Sponge-miRNAs
						
							ath-miR837-3p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1136175-1136291(+)
Potential amino acid sequence	MAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIGTVASGV AKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLKSGV DVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIGTVA SGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLK SGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQINPNAKVSVKLVAEAGIG TVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLTETHQTLIANGLRERVILRVDG GLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQ(+)
Sponge-miRNAs	ath-miR837-3p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT5G04140_circ_g.36

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