Plant Circular RNA Database

Detail information of Os01g0729100_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os01g0729100_circ_g.1
					
						ID in PlantcircBase
						osa_circ_003761
					
						Alias
						Os_ciR2345
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 30400528-30401330  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os01g0729100						
					
						Parent gene annotation
						
							Protein of unknown function DUF92, transmembrane family protein.
 (Os01t0729100-01);Similar to uncharacterized conserved membrane
 protein. (Os01t0729100-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0729100_circ_g.2 						
					
						Support reads
						4
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0729100-01:3
Os01t0729100-02:3

General Information
CircRNA Name	Os01g0729100_circ_g.1
ID in PlantcircBase	osa_circ_003761
Alias	Os_ciR2345
Organism	Oryza sativa
Position	chr1: 30400528-30401330 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os01g0729100
Parent gene annotation	Protein of unknown function DUF92, transmembrane family protein. (Os01t0729100-01);Similar to uncharacterized conserved membrane protein. (Os01t0729100-02)
Parent gene strand	+
Alternative splicing	Os01g0729100_circ_g.2
Support reads	4
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0729100-01:3 Os01t0729100-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATT
GGTGCCACACTACAAGATAAAGAAGGTTTTGAATGGggcacagcagcaactaagttgaaaataa
agcagaaagaagctttgggggtggcggagaaaagaggaggaaggagagggactggaagtgttat
tggttcca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 483
Transcript exons: 30400528-30400781,30400917-30401040,30401226-30401330
GGCACAGCAGCAACTAAGTTGAAAATAAAGCAGAAAGAAGCTTTGGGGGTGGCGGAGAAAAGAG
GAGGAAGGAGAGGGACTGGAAGTGTTATTGGTTCCAGTGCTGCTGGTTGTGTCTGTGCTCTGCT
ATCAATCTACAATGTAGGCAGTGCAGCGTTGGCTGAGCTCTGGAGACTTGGCTTCGTTGCTAGT
TATTGTACTAAACTAAGTGACACAGTTTCCAGTGAAATAGGGAAGGCCTACGGAAGAACAACGT
ACCTGGTGACAACACTTAAGGTTGTTCCGCGAGGTACAGAAGGTGCGATCAGTATTGAGGGTAC
TCTAGCTGGAATTCTTGCATCGATAATTCTTGCAAGTGTTGGTTATCTCCTGGGGCAGGTTAAT
GTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATTG
GTGCCACACTACAAGATAAAGAAGGTTTTGAATGG
						
						Genomic sequence
						
							GGCACAGCAGCAACTAAGTTGAAAATAAAGCAGAAAGAAGCTTTGGGGGTGGCGGAGAAAAGAG
GAGGAAGGAGAGGGACTGGAAGTGTTATTGGTTCCAGTGCTGCTGGTTGTGTCTGTGCTCTGCT
ATCAATCTACAATGTAGGCAGTGCAGCGTTGGCTGAGCTCTGGAGACTTGGCTTCGTTGCTAGT
TATTGTACTAAACTAAGTGACACAGTTTCCAGTGAAATAGGGAAGGCCTACGGAAGAACAACGT
AAGATTCCTGTCACTACACAGCCTTCAAATAATTCATGGTTCCACATCCATAAACTTGTGTATA
TATTTGTGCACTCTTAGTGAACTCAATGAACTGTACTTAGAGCTCAGTTTTGGTCTTTTTGTAG
AGTAGGTACCTGGTGACAACACTTAAGGTTGTTCCGCGAGGTACAGAAGGTGCGATCAGTATTG
AGGGTACTCTAGCTGGAATTCTTGCATCGATAATTCTTGCAAGTGTTGGTTATCTCCTGGGGCA
GGTAAATTATTTATACAGTAAACTTTGCAGAACAGCAGATATTTTGGAAAACCTACAGATTTAG
CACTGAATTGTCACAGAACTTATATGGAAATGACAGTGATCAGTGAATATATAGTCCTTACCGT
TTGCTCCAATACACTCGTATGATTATTGAAAAAAATAAAACCATGCATTTTATCGCAGGTTAAT
GTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATTG
GTGCCACACTACAAGATAAAGAAGGTTTTGAATGG

Sequences
Splice junction sequence	TGTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATT GGTGCCACACTACAAGATAAAGAAGGTTTTGAATGGggcacagcagcaactaagttgaaaataa agcagaaagaagctttgggggtggcggagaaaagaggaggaaggagagggactggaagtgttat tggttcca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 483 Transcript exons: 30400528-30400781,30400917-30401040,30401226-30401330 GGCACAGCAGCAACTAAGTTGAAAATAAAGCAGAAAGAAGCTTTGGGGGTGGCGGAGAAAAGAG GAGGAAGGAGAGGGACTGGAAGTGTTATTGGTTCCAGTGCTGCTGGTTGTGTCTGTGCTCTGCT ATCAATCTACAATGTAGGCAGTGCAGCGTTGGCTGAGCTCTGGAGACTTGGCTTCGTTGCTAGT TATTGTACTAAACTAAGTGACACAGTTTCCAGTGAAATAGGGAAGGCCTACGGAAGAACAACGT ACCTGGTGACAACACTTAAGGTTGTTCCGCGAGGTACAGAAGGTGCGATCAGTATTGAGGGTAC TCTAGCTGGAATTCTTGCATCGATAATTCTTGCAAGTGTTGGTTATCTCCTGGGGCAGGTTAAT GTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATTG GTGCCACACTACAAGATAAAGAAGGTTTTGAATGG
Genomic sequence	GGCACAGCAGCAACTAAGTTGAAAATAAAGCAGAAAGAAGCTTTGGGGGTGGCGGAGAAAAGAG GAGGAAGGAGAGGGACTGGAAGTGTTATTGGTTCCAGTGCTGCTGGTTGTGTCTGTGCTCTGCT ATCAATCTACAATGTAGGCAGTGCAGCGTTGGCTGAGCTCTGGAGACTTGGCTTCGTTGCTAGT TATTGTACTAAACTAAGTGACACAGTTTCCAGTGAAATAGGGAAGGCCTACGGAAGAACAACGT AAGATTCCTGTCACTACACAGCCTTCAAATAATTCATGGTTCCACATCCATAAACTTGTGTATA TATTTGTGCACTCTTAGTGAACTCAATGAACTGTACTTAGAGCTCAGTTTTGGTCTTTTTGTAG AGTAGGTACCTGGTGACAACACTTAAGGTTGTTCCGCGAGGTACAGAAGGTGCGATCAGTATTG AGGGTACTCTAGCTGGAATTCTTGCATCGATAATTCTTGCAAGTGTTGGTTATCTCCTGGGGCA GGTAAATTATTTATACAGTAAACTTTGCAGAACAGCAGATATTTTGGAAAACCTACAGATTTAG CACTGAATTGTCACAGAACTTATATGGAAATGACAGTGATCAGTGAATATATAGTCCTTACCGT TTGCTCCAATACACTCGTATGATTATTGAAAAAAATAAAACCATGCATTTTATCGCAGGTTAAT GTATCACAAGGCGCAGTATGTGTCCTTGCATCCCAGATTGCAAATTTTTGTGAGAGTTATATTG GTGCCACACTACAAGATAAAGAAGGTTTTGAATGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002367* osi_circ_010670 						
					
						PMCS
						0.391498236

Conservation Information
Conserved circRNAs	osi_circ_002367* osi_circ_010670
PMCS	0.391498236

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							30401231-30400546(+)
						
						Potential amino acid sequence
						
							MYHKAQYVSLHPRLQIFVRVILVPHYKIKKVLNGAQQQLS*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	30401231-30400546(+)
Potential amino acid sequence	MYHKAQYVSLHPRLQIFVRVILVPHYKIKKVLNGAQQQLS*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os01g0729100_circ_g.1

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