Plant Circular RNA Database

Detail information of AT5G42220_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT5G42220_circ_g.5
					
						ID in PlantcircBase
						ath_circ_041998
					
						Alias
						Ath_circ_FC3637, Ath_circ_FC3638
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 16874694-16875293  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, CIRI-full
					
						Parent gene
						
							AT5G42220						
					
						Parent gene annotation
						
							AT5g42220/K5J14_2
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G42220_circ_g.2 AT5G42220_circ_g.3 AT5G42220_circ_g.4 AT5G42220_circ_g.6 AT5G42220_circ_g.7 AT5G42220_circ_g.8 AT5G42220_circ_g.9 AT5G42220_circ_g.10 AT5G42220_circ_g.11 						
					
						Support reads
						5/5
					
						Tissues
						whole_plant/seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G42220.2:3
AT5G42220.1:3

General Information
CircRNA Name	AT5G42220_circ_g.5
ID in PlantcircBase	ath_circ_041998
Alias	Ath_circ_FC3637, Ath_circ_FC3638
Organism	Arabidpsis thaliana
Position	chr5: 16874694-16875293 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, CIRI-full
Parent gene	AT5G42220
Parent gene annotation	AT5g42220/K5J14_2
Parent gene strand	+
Alternative splicing	AT5G42220_circ_g.2 AT5G42220_circ_g.3 AT5G42220_circ_g.4 AT5G42220_circ_g.6 AT5G42220_circ_g.7 AT5G42220_circ_g.8 AT5G42220_circ_g.9 AT5G42220_circ_g.10 AT5G42220_circ_g.11
Support reads	5/5
Tissues	whole_plant/seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G42220.2:3 AT5G42220.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGCGGCTCATTAACACTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTAT
ATATCTCCATCAGGACCGAATCCTATTATGGTTCAGgctatcagccagatacctcttctgctgg
atccggagggcgaccaagggaagagttgcctagaaatagacgaggtgcagcaacacctgaagca
ctctctgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 392
Transcript exons: 16874694-16874848,16874980-16875144,16875222-16875293
GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA
TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG
AGTGGTCTCGGTGTCTCTTCTTTATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTT
CAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCAACA
TTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCGCCG
GAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGACCGAATCCTATTA
TGGTTCAG
Transcript length: 469
Transcript exons: 16874694-16875293
GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA
TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG
AGTGGTCTCGGTGTCTCTTCTTTATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTT
CAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCAACA
TTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCGCCG
GTGCGTTTTGGGTCATTCCTTTCGTGCATTTTCCTATTCATTCTTGACATGCGGCTCATTAACA
CTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGA
CCGAATCCTATTATGGTTCAG
						
					

						Genomic sequence
						
							GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA
TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG
AGTGGTCTCGGTGTCTCTTCTTTATCGGTAAACACCTTCTTCACACTGCTTATTAATTGATTCC
ATTCTTTCTCTTTACAAGAACTGTTATGTATATGGTTACCATGCATTGTCATCTAATTATGTGC
TGTTTGTAATTTGCAAAAACATTGTTGTAGCATATTGCGGGACGTCTGGAGCAAGATGGTTCAT
CTTCAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCA
ACATTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCG
CCGGTGCGTTTTGGGTCATTCCTTTCGTGCATTTTCCTATTCATTCTTGACATGCGGCTCATTA
ACACTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCA
GGACCGAATCCTATTATGGTTCAG
						
					

			
		

Sequences
Splice junction sequence	TGCGGCTCATTAACACTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTAT ATATCTCCATCAGGACCGAATCCTATTATGGTTCAGgctatcagccagatacctcttctgctgg atccggagggcgaccaagggaagagttgcctagaaatagacgaggtgcagcaacacctgaagca ctctctgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 392 Transcript exons: 16874694-16874848,16874980-16875144,16875222-16875293 GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG AGTGGTCTCGGTGTCTCTTCTTTATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTT CAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCAACA TTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCGCCG GAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGACCGAATCCTATTA TGGTTCAG Transcript length: 469 Transcript exons: 16874694-16875293 GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG AGTGGTCTCGGTGTCTCTTCTTTATCGCATATTGCGGGACGTCTGGAGCAAGATGGTTCATCTT CAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCAACA TTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCGCCG GTGCGTTTTGGGTCATTCCTTTCGTGCATTTTCCTATTCATTCTTGACATGCGGCTCATTAACA CTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCAGGA CCGAATCCTATTATGGTTCAG
Genomic sequence	GCTATCAGCCAGATACCTCTTCTGCTGGATCCGGAGGGCGACCAAGGGAAGAGTTGCCTAGAAA TAGACGAGGTGCAGCAACACCTGAAGCACTCTCTGTTGTCCTAAGGAATGCACAACATTTACTG AGTGGTCTCGGTGTCTCTTCTTTATCGGTAAACACCTTCTTCACACTGCTTATTAATTGATTCC ATTCTTTCTCTTTACAAGAACTGTTATGTATATGGTTACCATGCATTGTCATCTAATTATGTGC TGTTTGTAATTTGCAAAAACATTGTTGTAGCATATTGCGGGACGTCTGGAGCAAGATGGTTCAT CTTCAGATCCTACTCTAAGGTCACAAATCCAGACAGAGGCCGTGCAAGTAGGTCTTGCCATGCA ACATTTAGGGGCCCTCCTACTGGAGCTTGGACGCACAATTTTGACATTGAGAATGGCACCGTCG CCGGTGCGTTTTGGGTCATTCCTTTCGTGCATTTTCCTATTCATTCTTGACATGCGGCTCATTA ACACTTTGATCTACAGGAATTATCCTATGTGAATGCAGGGCCTGCTGTGTATATATCTCCATCA GGACCGAATCCTATTATGGTTCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.333669485

Conservation Information
Conserved circRNAs	NA
PMCS	0.333669485

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Chu et al., 2017;Pan et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G42220_circ_g.5

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