Plant Circular RNA Database

Detail information of Os03g0158500_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0158500_circ_g.4
					
						ID in PlantcircBase
						osa_circ_018035
					
						Alias
						Os_ciR3863
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 3127937-3128137  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os03g0158500						
					
						Parent gene annotation
						
							YT521-B-like protein family protein. (Os03t0158500-01);Similar t
o YT521-B-like family protein, expressed. (Os03t0158500-02);Simi
lar to YT521-B-like family protein, expressed. (Os03t0158500-03)
;Similar to YT521-B-like family protein, expressed. (Os03t015850
0-04)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0158500_circ_g.5 Os03g0158500_circ_g.6 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0158500-02:1
Os03t0158500-01:1
Os03t0158500-03:1

General Information
CircRNA Name	Os03g0158500_circ_g.4
ID in PlantcircBase	osa_circ_018035
Alias	Os_ciR3863
Organism	Oryza sativa
Position	chr3: 3127937-3128137 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os03g0158500
Parent gene annotation	YT521-B-like protein family protein. (Os03t0158500-01);Similar t o YT521-B-like family protein, expressed. (Os03t0158500-02);Simi lar to YT521-B-like family protein, expressed. (Os03t0158500-03) ;Similar to YT521-B-like family protein, expressed. (Os03t015850 0-04)
Parent gene strand	-
Alternative splicing	Os03g0158500_circ_g.5 Os03g0158500_circ_g.6
Support reads	2
Tissues	root
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0158500-02:1 Os03t0158500-01:1 Os03t0158500-03:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGTTTTGTATGAGTTTCCATACTGGCTGTAAGACCGGGTGCTCGGGTACATCCTATTCGGATAT
GTAGGAGCAGCTGATCCTGTTGTAGTTGTAGGTCTTctgttatccatactaagaccccaccgac
catatagcctggaatcataaccattactaccatagccattacttccataaccatttgtgctgta
cgaaagac

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CTGTTATCCATACTAAGACCCCACCGACCATATAGCCTGGAATCATAACCATTACTACCATAGC
CATTACTTCCATAACCATTTGTGCTGTACGAAAGACCAGTTTTGTATGAGTTTCCATACTGGCT
GTAAGACCGGGTGCTCGGGTACATCCTATTCGGATATGTAGGAGCAGCTGATCCTGTTGTAGTT
GTAGGTCTT

Sequences
Splice junction sequence	AGTTTTGTATGAGTTTCCATACTGGCTGTAAGACCGGGTGCTCGGGTACATCCTATTCGGATAT GTAGGAGCAGCTGATCCTGTTGTAGTTGTAGGTCTTctgttatccatactaagaccccaccgac catatagcctggaatcataaccattactaccatagccattacttccataaccatttgtgctgta cgaaagac
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGTTATCCATACTAAGACCCCACCGACCATATAGCCTGGAATCATAACCATTACTACCATAGC CATTACTTCCATAACCATTTGTGCTGTACGAAAGACCAGTTTTGTATGAGTTTCCATACTGGCT GTAAGACCGGGTGCTCGGGTACATCCTATTCGGATATGTAGGAGCAGCTGATCCTGTTGTAGTT GTAGGTCTT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004558* osi_circ_013699 						
					
						PMCS
						0.676107557

Conservation Information
Conserved circRNAs	osi_circ_004558* osi_circ_013699
PMCS	0.676107557

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os03g0158500_circ_g.4

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