Plant Circular RNA Database

Detail information of Os12g0448900_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os12g0448900_circ_g.5
					
						ID in PlantcircBase
						osa_circ_011427
					
						Alias
						Os_ciR2834
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 15332964-15334232  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os12g0448900						
					
						Parent gene annotation
						
							Fatty acid alpha-dioxygenase family, Enzyme that oxygenates fatt
y acids into 2R-hydroperoxides (Os12t0448900-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os12g0448900_circ_g.6 						
					
						Support reads
						4
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0448900-01:4

General Information
CircRNA Name	Os12g0448900_circ_g.5
ID in PlantcircBase	osa_circ_011427
Alias	Os_ciR2834
Organism	Oryza sativa
Position	chr12: 15332964-15334232 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os12g0448900
Parent gene annotation	Fatty acid alpha-dioxygenase family, Enzyme that oxygenates fatt y acids into 2R-hydroperoxides (Os12t0448900-01)
Parent gene strand	-
Alternative splicing	Os12g0448900_circ_g.6
Support reads	4
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0448900-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTATTCCATCAGAATTCGTAGGCAGTTCTTTTGTGGGGTGGAACTTGAACGATTTGAGTGGGCA
CTCATTGGCCACTTCTTTTGGAGCTGTGATTCCAATtcttccaggcatggtggggaattatttg
catcgggttgccctgtcggatccctgagcttgagagtgcttggtattagggagtgcattctgta
aacactgg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 722
Transcript exons: 15332964-15333191,15333363-15333508,15333709-15333922,15
334099-15334232
TCTTCCAGGCATGGTGGGGAATTATTTGCATCGGGTTGCCCTGTCGGATCCCTGAGCTTGAGAG
TGCTTGGTATTAGGGAGTGCATTCTGTAAACACTGGTAAACTCTTCAGTCAAAGAATAAGGTAC
GCCATGGTTGTTCGGCTTCTTCAGTCCCACAAGCCCACCTAATATCGGTCCTCCTATGTGACCA
AATGTGTCTTTTATTTTCTTACCCAATAATCCATACCAATTTGCGCGCATTGCAGCTCTCATGG
TTTTTGTCTTGAGAAGCTCGACAGTCCAATCAATAGTGTGAACCTTTGCAATAACAGCAGAAGT
GACCAATTTGGCATACCGATATAATTCTTCGTCTGATAGGTTGGGATGTTCTTCCTTTATTGCA
TCACAAACTGCATTGTGTTCCTTAACAAAAAGAGCTTGTAAGATCGAAACACCAGCCCAACTGT
TGCGAATGTCGCCGGATAATGCCACACCGTTCTCCTTATGCAAAAGAAGACCGTCATCTCCGAT
CACTAGTTTTCCATCAACATAAGTCCTGAGCTTTTCTGCCCTTTCCTCATTATTACCATATACT
GCACTCCCATCCCACCAAGCTGTGCGGATATTGTAGTGACCAATCTTTATTCCATCAGAATTCG
TAGGCAGTTCTTTTGTGGGGTGGAACTTGAACGATTTGAGTGGGCACTCATTGGCCACTTCTTT
TGGAGCTGTGATTCCAAT
						
					

						Genomic sequence
						
							TCTTCCAGGCATGGTGGGGAATTATTTGCATCGGGTTGCCCTGTCGGATCCCTGAGCTTGAGAG
TGCTTGGTATTAGGGAGTGCATTCTGTAAACACTGGTAAACTCTTCAGTCAAAGAATAAGGTAC
GCCATGGTTGTTCGGCTTCTTCAGTCCCACAAGCCCACCTAATATCGGTCCTCCTATGTGACCA
AATGTGTCTTTTATTTTCTTACCCAATAATCCATACCTTCACAAAAGAATCAGAATATCAGGTA
AATTGTACACATTCTCACTAATTTAATTAATTTGAATTTGCAGATGTAAATAAAATTTCCACTT
GGAAAAGTAATACCCGCATCAATGGTTATATTTTTTTTCTTTCGGCAAATATGAGAAAATGAGA
TAAATGCATCCTCACCAATTTGCGCGCATTGCAGCTCTCATGGTTTTTGTCTTGAGAAGCTCGA
CAGTCCAATCAATAGTGTGAACCTTTGCAATAACAGCAGAAGTGACCAATTTGGCATACCGATA
TAATTCTTCGTCTGATAGGTTGGGATGTTCTTCCTGAGATTAAAAAAAACATTTGTAATCTTTT
CTTCATATTTATAGCATAAAGAAAACAAATTAATGTTTTGGTAAATAACAGTTTATATTCTTCC
TAAAACTAAACAAATAGACAATCAGTGGCAAGCTAGTCAAGAACAGCAAATTATATTCTTCCTA
AAACTAAACAAATAGAAAATAGTGATGATATATTCGCTTACCTTTATTGCATCACAAACTGCAT
TGTGTTCCTTAACAAAAAGAGCTTGTAAGATCGAAACACCAGCCCAACTGTTGCGAATGTCGCC
GGATAATGCCACACCGTTCTCCTTATGCAAAAGAAGACCGTCATCTCCGATCACTAGTTTTCCA
TCAACATAAGTCCTGAGCTTTTCTGCCCTTTCCTCATTATTACCATATACTGCACTCCCATCCC
TTGGTATATATAATTATAGAAATTGCAAAAAAAAAATTACGACATTTTTTATGGGCAAATATGC
ATTGATTTATATATATACATCGATTGTCTCTATTTTTTAAGTTCATTCTTTGTGCTTAAGATAA
ATTTCTGATAAAGATCTTAACTCGAGAAAATCTTAAGCATTACTCACCACCAAGCTGTGCGGAT
ATTGTAGTGACCAATCTTTATTCCATCAGAATTCGTAGGCAGTTCTTTTGTGGGGTGGAACTTG
AACGATTTGAGTGGGCACTCATTGGCCACTTCTTTTGGAGCTGTGATTCCAAT
						
					

			
		

Sequences
Splice junction sequence	TTATTCCATCAGAATTCGTAGGCAGTTCTTTTGTGGGGTGGAACTTGAACGATTTGAGTGGGCA CTCATTGGCCACTTCTTTTGGAGCTGTGATTCCAATtcttccaggcatggtggggaattatttg catcgggttgccctgtcggatccctgagcttgagagtgcttggtattagggagtgcattctgta aacactgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 722 Transcript exons: 15332964-15333191,15333363-15333508,15333709-15333922,15 334099-15334232 TCTTCCAGGCATGGTGGGGAATTATTTGCATCGGGTTGCCCTGTCGGATCCCTGAGCTTGAGAG TGCTTGGTATTAGGGAGTGCATTCTGTAAACACTGGTAAACTCTTCAGTCAAAGAATAAGGTAC GCCATGGTTGTTCGGCTTCTTCAGTCCCACAAGCCCACCTAATATCGGTCCTCCTATGTGACCA AATGTGTCTTTTATTTTCTTACCCAATAATCCATACCAATTTGCGCGCATTGCAGCTCTCATGG TTTTTGTCTTGAGAAGCTCGACAGTCCAATCAATAGTGTGAACCTTTGCAATAACAGCAGAAGT GACCAATTTGGCATACCGATATAATTCTTCGTCTGATAGGTTGGGATGTTCTTCCTTTATTGCA TCACAAACTGCATTGTGTTCCTTAACAAAAAGAGCTTGTAAGATCGAAACACCAGCCCAACTGT TGCGAATGTCGCCGGATAATGCCACACCGTTCTCCTTATGCAAAAGAAGACCGTCATCTCCGAT CACTAGTTTTCCATCAACATAAGTCCTGAGCTTTTCTGCCCTTTCCTCATTATTACCATATACT GCACTCCCATCCCACCAAGCTGTGCGGATATTGTAGTGACCAATCTTTATTCCATCAGAATTCG TAGGCAGTTCTTTTGTGGGGTGGAACTTGAACGATTTGAGTGGGCACTCATTGGCCACTTCTTT TGGAGCTGTGATTCCAAT
Genomic sequence	TCTTCCAGGCATGGTGGGGAATTATTTGCATCGGGTTGCCCTGTCGGATCCCTGAGCTTGAGAG TGCTTGGTATTAGGGAGTGCATTCTGTAAACACTGGTAAACTCTTCAGTCAAAGAATAAGGTAC GCCATGGTTGTTCGGCTTCTTCAGTCCCACAAGCCCACCTAATATCGGTCCTCCTATGTGACCA AATGTGTCTTTTATTTTCTTACCCAATAATCCATACCTTCACAAAAGAATCAGAATATCAGGTA AATTGTACACATTCTCACTAATTTAATTAATTTGAATTTGCAGATGTAAATAAAATTTCCACTT GGAAAAGTAATACCCGCATCAATGGTTATATTTTTTTTCTTTCGGCAAATATGAGAAAATGAGA TAAATGCATCCTCACCAATTTGCGCGCATTGCAGCTCTCATGGTTTTTGTCTTGAGAAGCTCGA CAGTCCAATCAATAGTGTGAACCTTTGCAATAACAGCAGAAGTGACCAATTTGGCATACCGATA TAATTCTTCGTCTGATAGGTTGGGATGTTCTTCCTGAGATTAAAAAAAACATTTGTAATCTTTT CTTCATATTTATAGCATAAAGAAAACAAATTAATGTTTTGGTAAATAACAGTTTATATTCTTCC TAAAACTAAACAAATAGACAATCAGTGGCAAGCTAGTCAAGAACAGCAAATTATATTCTTCCTA AAACTAAACAAATAGAAAATAGTGATGATATATTCGCTTACCTTTATTGCATCACAAACTGCAT TGTGTTCCTTAACAAAAAGAGCTTGTAAGATCGAAACACCAGCCCAACTGTTGCGAATGTCGCC GGATAATGCCACACCGTTCTCCTTATGCAAAAGAAGACCGTCATCTCCGATCACTAGTTTTCCA TCAACATAAGTCCTGAGCTTTTCTGCCCTTTCCTCATTATTACCATATACTGCACTCCCATCCC TTGGTATATATAATTATAGAAATTGCAAAAAAAAAATTACGACATTTTTTATGGGCAAATATGC ATTGATTTATATATATACATCGATTGTCTCTATTTTTTAAGTTCATTCTTTGTGCTTAAGATAA ATTTCTGATAAAGATCTTAACTCGAGAAAATCTTAAGCATTACTCACCACCAAGCTGTGCGGAT ATTGTAGTGACCAATCTTTATTCCATCAGAATTCGTAGGCAGTTCTTTTGTGGGGTGGAACTTG AACGATTTGAGTGGGCACTCATTGGCCACTTCTTTTGGAGCTGTGATTCCAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.424028677

Conservation Information
Conserved circRNAs	NA
PMCS	0.424028677

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							15333390-15334135(-)
15332996-15334200(-)
15332977-15334135(-)
						
						Potential amino acid sequence
						
							MRAAMRANWYGLLGKKIKDTFGHIGGPILGGLVGLKKPNNHGVPYSLTEEFTSVYRMHSLIPST
LKLRDPTGQPDANNSPPCLEELESQLQKKWPMSAHSNRSSSTPQKNCLRILME*(-)
MQIIPHHAWKNWNHSSKRSGQ*(-)
MPGRIGITAPKEVANECPLKSFKFHPTKELPTNSDGIKIGHYNIRTAWWDGSAVYGNNEERAEK
LRTYVDGKLVIGDDGLLLHKENGVALSGDIRNSWAGVSILQALFVKEHNAVCDAIKEEHPNLSD
EELYRYAKLVTSAVIAKVHTIDWTVELLKTKTMRAAMRANWYGLLGKKIKDTFGHIGGPILGGL
VGLKKPNNHGVPYSLTEEFTSVYRMHSLIPSTLKLRDPTGQPDANNSPPCLEELESQLQKKWPM
SAHSNRSSSTPQKNCLRILME*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	15333390-15334135(-) 15332996-15334200(-) 15332977-15334135(-)
Potential amino acid sequence	MRAAMRANWYGLLGKKIKDTFGHIGGPILGGLVGLKKPNNHGVPYSLTEEFTSVYRMHSLIPST LKLRDPTGQPDANNSPPCLEELESQLQKKWPMSAHSNRSSSTPQKNCLRILME(-) MQIIPHHAWKNWNHSSKRSGQ(-) MPGRIGITAPKEVANECPLKSFKFHPTKELPTNSDGIKIGHYNIRTAWWDGSAVYGNNEERAEK LRTYVDGKLVIGDDGLLLHKENGVALSGDIRNSWAGVSILQALFVKEHNAVCDAIKEEHPNLSD EELYRYAKLVTSAVIAKVHTIDWTVELLKTKTMRAAMRANWYGLLGKKIKDTFGHIGGPILGGL VGLKKPNNHGVPYSLTEEFTSVYRMHSLIPSTLKLRDPTGQPDANNSPPCLEELESQLQKKWPM SAHSNRSSSTPQKNCLRILME*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os12g0448900_circ_g.5

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