Plant Circular RNA Database

Detail information of Os07g0496200_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os07g0496200_circ_g.1
					
						ID in PlantcircBase
						osa_circ_033912
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 18588747-18590252  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os07g0496200						
					
						Parent gene annotation
						
							Similar to NAD-dependent malic enzyme 62 kDa isoform, mitochondr
ial precursor (EC 1.1.1.39) (NAD-ME). (Os07t0496200-01);Similar 
to NAD-dependent malic enzyme 62 kDa isoform, mitochondrial prec
ursor (EC 1.1.1.39) (NAD-ME). (Os07t0496200-02);Similar to Malic
 enzyme. (Os07t0496200-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0496200-02:4
Os07t0496200-03:3
Os07t0496200-01:4

General Information
CircRNA Name	Os07g0496200_circ_g.1
ID in PlantcircBase	osa_circ_033912
Alias	NA
Organism	Oryza sativa
Position	chr7: 18588747-18590252 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os07g0496200
Parent gene annotation	Similar to NAD-dependent malic enzyme 62 kDa isoform, mitochondr ial precursor (EC 1.1.1.39) (NAD-ME). (Os07t0496200-01);Similar to NAD-dependent malic enzyme 62 kDa isoform, mitochondrial prec ursor (EC 1.1.1.39) (NAD-ME). (Os07t0496200-02);Similar to Malic enzyme. (Os07t0496200-03)
Parent gene strand	+
Alternative splicing	NA
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0496200-02:4 Os07t0496200-03:3 Os07t0496200-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTACCATTGATTTAAAACTGTCTTCACTTTTTTTCTAGGTTCTTCCGGTCATGATTGATGTGG
GAACAAACAATGAGAAGCTCCTCAAGGATCCTCTGTtgcttgacatgcaacggctgcagaaata
tgcgagagatggcccatcggatacttacccgttagcaaaatggcgtatccttaacaggctgcat
gacagaaa

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							TGCTTGACATGCAACGGCTGCAGAAATATGCGAGAGATGGCCCATCGGATACTTACCCGTTAGC
AAAATGGCGTATCCTTAACAGGCTGCATGACAGAAATGAAACAATGTATTACAAGGTTTGTAAC
TTTACAACTCATACATTTGTGTGCAACCTTATTTCTATTGATCATGTATGCTCTCACCATTTAA
TACGCACAATTTGGACTGTTTACGGGACACTGTATGATTACACATGTTCGTTGCTAACTTCGTT
TCTACTATAATCTCAATTGTTGTTAACTTTAGCAGGCTGAGGTGTGCCTTTTCAAAGTGGTTAC
CTGATTTCCATATTTTTTTCATCTTTTTAATAGGTCTTAATAGATAATATTGAGGAACATGCAC
CTATTGTTTACACGCCGACTGTTGGACTTGTTTGTCAAAACTATAGTGGTTTATTTAGACGGCC
TCGGGGAATGTATTTTAGTGCAGAAGATCGTGGGGAAATGATGTCCATGGTGTACAACTGGCCA
GCTGACCAGGTACTCATTTGTGTTCATCCAGTTACTGATTTTACATTTAGCAGCTGAGCTCTTT
CATTGGTTTAATCTTTAGTGGTATAATTCAGTAACGCTATCTCAGAGCATAAGACAGTCTACCT
TTTGCTTTTGATTTTTGAACATCTCTCAAGAAAAAAAATGTCATATAATCTCAAAGTAAATAAA
ATATGTCCGTTCCTTTGCAGGTTGACATGATAGTAGTGACTGATGGAAGCAGGATATTGGGCCT
GGGTGATCTCGGGGTGCATGGTATTGGTATTGCCATTGGAAAGTTGGATCTATATGTTGCTGCT
GCTGGAATAAATCCCCAGAGGGTAAAAACTTTTGGTTTATGGTACCTTAGAAGATTGTTAGACT
CTTCCCTTTCTCCCTCTTCTAATTTTACATCTCCCGTGCTTTTTTGTAATGACTTTTGTTGCAG
GAGTTAATCAGCTAAACTAAGAAAGTATAGTGAAGCTTGTTTGCTGAAGAGGTTAAAAAAACTA
TTAGTGGATGCTAAAAATACAGTTTCATATGTATCATCTATAGGGATTTCGTTTGATCAGTAAT
GATGGTCCTGCTACTTATGTGATAATGTGTCAGTTAGGCCCATTTTATTATAGATTGGGGAGAT
AAAATACCTGCTAGAGTTTTACTATCTCGACTCATTTCTGGACACAGCTAAAATGAAATATGTT
ACTAAAGTGTGAATTCATAGGGTCGGAAAAATAACAAAGCTTCAAATTAAGATACATTGACTTA
AATTACTGTACCTTAGCAAAAGTGGTACCAATGCCTAGTGCTTAAATGTTATGGATTCTATAAG
TACTCTACTGCTATTCATTTCTTGTTGTTTGCCTACTGCTCATGTGGCTAGCAGAATTTATATT
TACCATTGATTTAAAACTGTCTTCACTTTTTTTCTAGGTTCTTCCGGTCATGATTGATGTGGGA
ACAAACAATGAGAAGCTCCTCAAGGATCCTCTGT
						
					

			
		

Sequences
Splice junction sequence	TTTACCATTGATTTAAAACTGTCTTCACTTTTTTTCTAGGTTCTTCCGGTCATGATTGATGTGG GAACAAACAATGAGAAGCTCCTCAAGGATCCTCTGTtgcttgacatgcaacggctgcagaaata tgcgagagatggcccatcggatacttacccgttagcaaaatggcgtatccttaacaggctgcat gacagaaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TGCTTGACATGCAACGGCTGCAGAAATATGCGAGAGATGGCCCATCGGATACTTACCCGTTAGC AAAATGGCGTATCCTTAACAGGCTGCATGACAGAAATGAAACAATGTATTACAAGGTTTGTAAC TTTACAACTCATACATTTGTGTGCAACCTTATTTCTATTGATCATGTATGCTCTCACCATTTAA TACGCACAATTTGGACTGTTTACGGGACACTGTATGATTACACATGTTCGTTGCTAACTTCGTT TCTACTATAATCTCAATTGTTGTTAACTTTAGCAGGCTGAGGTGTGCCTTTTCAAAGTGGTTAC CTGATTTCCATATTTTTTTCATCTTTTTAATAGGTCTTAATAGATAATATTGAGGAACATGCAC CTATTGTTTACACGCCGACTGTTGGACTTGTTTGTCAAAACTATAGTGGTTTATTTAGACGGCC TCGGGGAATGTATTTTAGTGCAGAAGATCGTGGGGAAATGATGTCCATGGTGTACAACTGGCCA GCTGACCAGGTACTCATTTGTGTTCATCCAGTTACTGATTTTACATTTAGCAGCTGAGCTCTTT CATTGGTTTAATCTTTAGTGGTATAATTCAGTAACGCTATCTCAGAGCATAAGACAGTCTACCT TTTGCTTTTGATTTTTGAACATCTCTCAAGAAAAAAAATGTCATATAATCTCAAAGTAAATAAA ATATGTCCGTTCCTTTGCAGGTTGACATGATAGTAGTGACTGATGGAAGCAGGATATTGGGCCT GGGTGATCTCGGGGTGCATGGTATTGGTATTGCCATTGGAAAGTTGGATCTATATGTTGCTGCT GCTGGAATAAATCCCCAGAGGGTAAAAACTTTTGGTTTATGGTACCTTAGAAGATTGTTAGACT CTTCCCTTTCTCCCTCTTCTAATTTTACATCTCCCGTGCTTTTTTGTAATGACTTTTGTTGCAG GAGTTAATCAGCTAAACTAAGAAAGTATAGTGAAGCTTGTTTGCTGAAGAGGTTAAAAAAACTA TTAGTGGATGCTAAAAATACAGTTTCATATGTATCATCTATAGGGATTTCGTTTGATCAGTAAT GATGGTCCTGCTACTTATGTGATAATGTGTCAGTTAGGCCCATTTTATTATAGATTGGGGAGAT AAAATACCTGCTAGAGTTTTACTATCTCGACTCATTTCTGGACACAGCTAAAATGAAATATGTT ACTAAAGTGTGAATTCATAGGGTCGGAAAAATAACAAAGCTTCAAATTAAGATACATTGACTTA AATTACTGTACCTTAGCAAAAGTGGTACCAATGCCTAGTGCTTAAATGTTATGGATTCTATAAG TACTCTACTGCTATTCATTTCTTGTTGTTTGCCTACTGCTCATGTGGCTAGCAGAATTTATATT TACCATTGATTTAAAACTGTCTTCACTTTTTTTCTAGGTTCTTCCGGTCATGATTGATGTGGGA ACAAACAATGAGAAGCTCCTCAAGGATCCTCTGT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.158234318

Conservation Information
Conserved circRNAs	NA
PMCS	0.158234318

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18590212-18588806(+)
18588756-18590249(+)
18588758-18589173(-)
						
						Potential amino acid sequence
						
							MWEQTMRSSSRILCCLTCNGCRNMREMAHRILTR*(+)
MQRLQKYARDGPSDTYPLAKWRILNRLHDRNETMYYKVLIDNIEEHAPIVYTPTVGLVCQNYSG
LFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVA
AAGINPQRVLPVMIDVGTNNEKLLKDPLLLDMQRLQKYARDGPSDTYPLAKWRILNRLHDRNET
MYYKVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMI
VVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPLLLDMQ
RLQKYARDGPSDTYPLAKWRILNRLHDRNETMYYKVLIDNIEEHAPIVYTPTVGLVCQNYSGLF
RRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAA
GINPQRVLPVMIDVGTNNEKLLKDPLLLDMQRLQKYARDGPSDTYPLAKWRILNRLHDRNETMY
YKVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVV
TDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL(+)
MSSNRGSLRSFSLFVPTSIMTGRTLWGFIPAAATYRSNFPMAIPIPCTPRSPRPNILLPSVTTI
MSTWSAGQLYTMDIISPRSSALKYIPRGRLNKPL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	18590212-18588806(+) 18588756-18590249(+) 18588758-18589173(-)
Potential amino acid sequence	MWEQTMRSSSRILCCLTCNGCRNMREMAHRILTR(+) MQRLQKYARDGPSDTYPLAKWRILNRLHDRNETMYYKVLIDNIEEHAPIVYTPTVGLVCQNYSG LFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVA AAGINPQRVLPVMIDVGTNNEKLLKDPLLLDMQRLQKYARDGPSDTYPLAKWRILNRLHDRNET MYYKVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMI VVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPLLLDMQ RLQKYARDGPSDTYPLAKWRILNRLHDRNETMYYKVLIDNIEEHAPIVYTPTVGLVCQNYSGLF RRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAA GINPQRVLPVMIDVGTNNEKLLKDPLLLDMQRLQKYARDGPSDTYPLAKWRILNRLHDRNETMY YKVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVV TDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL(+) MSSNRGSLRSFSLFVPTSIMTGRTLWGFIPAAATYRSNFPMAIPIPCTPRSPRPNILLPSVTTI MSTWSAGQLYTMDIISPRSSALKYIPRGRLNKPL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os07g0496200_circ_g.1

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