Plant Circular RNA Database

Detail information of Os02g0197200_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os02g0197200_circ_g.4
					
						ID in PlantcircBase
						osa_circ_013654
					
						Alias
						NA, 
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 5444834-5445220  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, CIRI-long
					
						Parent gene
						
							Os02g0197125						
					
						Parent gene annotation
						
							Hypothetical protein. (Os02t0197125-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0197200_circ_g.2 Os02g0197200_circ_g.3 Os02g0197200_circ_g.5 Os02g0197200_circ_g.6 Os02g0197200_circ_g.7 Os02g0197200_circ_g.8 Os02g0197200_circ_g.9 Os02g0197200_circ_g.10 						
					
						Support reads
						1
					
						Tissues
						shoot, leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0197200-01:3
Os02t0197125-00:2
Os02t0197200-01:3

General Information
CircRNA Name	Os02g0197200_circ_g.4
ID in PlantcircBase	osa_circ_013654
Alias	NA,
Organism	Oryza sativa
Position	chr2: 5444834-5445220 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, CIRI-long
Parent gene	Os02g0197125
Parent gene annotation	Hypothetical protein. (Os02t0197125-00)
Parent gene strand	-
Alternative splicing	Os02g0197200_circ_g.2 Os02g0197200_circ_g.3 Os02g0197200_circ_g.5 Os02g0197200_circ_g.6 Os02g0197200_circ_g.7 Os02g0197200_circ_g.8 Os02g0197200_circ_g.9 Os02g0197200_circ_g.10
Support reads	1
Tissues	shoot, leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0197200-01:3 Os02t0197125-00:2 Os02t0197200-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TAAAAACTACATCATCTTTACTGTATCTAATGCTATGCAATTACATGCAGCTGGTACCTATGGC
TTTTCGTTATTGTCTTCCTGCTACTGAATATAAATGatctcattcttgaagcagttttatgatt
ctgttggtaaacctgattatcaagtacttagatcagcttttgttcaggtcagatgcatctctga
gtaacttc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 214
Transcript exons: 5444834-5444908,5444996-5445084,5445171-5445220
ATCTCATTCTTGAAGCAGTTTTATGATTCTGTTGGTAAACCTGATTATCAAGTACTTAGATCAG
CTTTTGTTCAGAGGCACTACCCAAACCGTCCTGATTTTGATTTCCACAAGTACATGGTTCGTGC
TCTTGAGCATGATTTTAAAGAAGTAGTTGGAATCAGCTGGTACCTATGGCTTTTCGTTATTGTC
TTCCTGCTACTGAATATAAATG
						
						Genomic sequence
						
							ATCTCATTCTTGAAGCAGTTTTATGATTCTGTTGGTAAACCTGATTATCAAGTACTTAGATCAG
CTTTTGTTCAGGTCAGATGCATCTCTGAGTAACTTCAGCTCAATCATAAGTGTCATTGTACTAT
TTTTGAAGCTAATAGGACATAATTTTTTCCCCAGAGGCACTACCCAAACCGTCCTGATTTTGAT
TTCCACAAGTACATGGTTCGTGCTCTTGAGCATGATTTTAAAGAAGTAGTTGGAATCAGGTTAG
TATCTTGGTTAAAATACTAACTTTACTTGATTAAAAACTACATCATCTTTACTGTATCTAATGC
TATGCAATTACATGCAGCTGGTACCTATGGCTTTTCGTTATTGTCTTCCTGCTACTGAATATAA
ATG

Sequences
Splice junction sequence	TAAAAACTACATCATCTTTACTGTATCTAATGCTATGCAATTACATGCAGCTGGTACCTATGGC TTTTCGTTATTGTCTTCCTGCTACTGAATATAAATGatctcattcttgaagcagttttatgatt ctgttggtaaacctgattatcaagtacttagatcagcttttgttcaggtcagatgcatctctga gtaacttc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 214 Transcript exons: 5444834-5444908,5444996-5445084,5445171-5445220 ATCTCATTCTTGAAGCAGTTTTATGATTCTGTTGGTAAACCTGATTATCAAGTACTTAGATCAG CTTTTGTTCAGAGGCACTACCCAAACCGTCCTGATTTTGATTTCCACAAGTACATGGTTCGTGC TCTTGAGCATGATTTTAAAGAAGTAGTTGGAATCAGCTGGTACCTATGGCTTTTCGTTATTGTC TTCCTGCTACTGAATATAAATG
Genomic sequence	ATCTCATTCTTGAAGCAGTTTTATGATTCTGTTGGTAAACCTGATTATCAAGTACTTAGATCAG CTTTTGTTCAGGTCAGATGCATCTCTGAGTAACTTCAGCTCAATCATAAGTGTCATTGTACTAT TTTTGAAGCTAATAGGACATAATTTTTTCCCCAGAGGCACTACCCAAACCGTCCTGATTTTGAT TTCCACAAGTACATGGTTCGTGCTCTTGAGCATGATTTTAAAGAAGTAGTTGGAATCAGGTTAG TATCTTGGTTAAAATACTAACTTTACTTGATTAAAAACTACATCATCTTTACTGTATCTAATGC TATGCAATTACATGCAGCTGGTACCTATGGCTTTTCGTTATTGTCTTCCTGCTACTGAATATAA ATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.195466193

Conservation Information
Conserved circRNAs	NA
PMCS	0.195466193

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5445039-5444856(+)
5445219-5444856(+)
5444842-5445003(-)
						
						Potential amino acid sequence
						
							MVRALEHDFKEVVGISWYLWLFVIVFLLLNINDLILEAVL*(+)
MISFLKQFYDSVGKPDYQVLRSAFVQRHYPNRPDFDFHKYMVRALEHDFKEVVGISWYLWLFVI
VFLLLNINDLILEAVL*(+)
MRSFIFSSRKTITKSHRYQLIPTTSLKSCSRARTMYLWKSKSGRFG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5445039-5444856(+) 5445219-5444856(+) 5444842-5445003(-)
Potential amino acid sequence	MVRALEHDFKEVVGISWYLWLFVIVFLLLNINDLILEAVL(+) MISFLKQFYDSVGKPDYQVLRSAFVQRHYPNRPDFDFHKYMVRALEHDFKEVVGISWYLWLFVI VFLLLNINDLILEAVL(+) MRSFIFSSRKTITKSHRYQLIPTTSLKSCSRARTMYLWKSKSGRFG*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017; this study

circRNA

Detail information of Os02g0197200_circ_g.4

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