Plant Circular RNA Database

Detail information of AT5G19730_circ_g.1

			
						 General Information
					
						CircRNA Name
						AT5G19730_circ_g.1
					
						ID in PlantcircBase
						ath_circ_039299
					
						Alias
						At_ciR436, AT5G19730_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 6671815-6672618  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI2
					
						Parent gene
						
							AT5G19730						
					
						Parent gene annotation
						
							Probable pectinesterase 53
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G19730_circ_g.2 						
					
						Support reads
						19/16
					
						Tissues
						leaf/root, leaf, aerial
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G19730.1:3

General Information
CircRNA Name	AT5G19730_circ_g.1
ID in PlantcircBase	ath_circ_039299
Alias	At_ciR436, AT5G19730_C1
Organism	Arabidpsis thaliana
Position	chr5: 6671815-6672618 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, MapSplice, PcircRNA_finder, circRNA_finder, find_circ, CIRI2
Parent gene	AT5G19730
Parent gene annotation	Probable pectinesterase 53
Parent gene strand	+
Alternative splicing	AT5G19730_circ_g.2
Support reads	19/16
Tissues	leaf/root, leaf, aerial
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G19730.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACATGGACAACATCATCCTCCCTAG
AGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGATggagaaggtgagcatacccccactgaag
gcatttataacaatagaaggagaaggagcagagaagacaacagtggaatggggagacacagcac
aaacccct

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 154
Transcript exons: 6671815-6671938,6672589-6672618
GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG
ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCGTAC
AATTGGGGAGACCCTTCCCGTGAGAT
Transcript length: 260
Transcript exons: 6671815-6671963,6672508-6672618
GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG
ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT
ACAACTCGGCCTCGTTCGCGGTCTTGGGAGGGCATGGGGTCCCTTCTCAAGAGTCGTTTTTGCT
TACACTTACATGGACAACATCATCCTCCCTAGAGGCTGGTACAATTGGGGAGACCCTTCCCGTG
AGAT
Transcript length: 633
Transcript exons: 6671815-6672004,6672098-6672304,6672383-6672618
GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG
ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT
ACAACTCGGCCTCGTTCGCGGTCAACTCTCCTTTCTTTGTCGCTAAGAATATCACATTCAGGAA
CACGACCCCAGTCCCCTTGCCCGGTGCAGTTGGTAAACAGGCGGTAGCATTGAGGGTGTCGGCA
GACAATGCCGCGTTTTTTGGGTGTAGAATGCTTGGTGCTCAAGACACTCTCTATGACCATTTGG
GAAGACATTATTACAAAGATTGTTACATTGAGGGATCAGTGGACTTCATCTTTGGAAATGCCCT
TTCTCTTTATGAAGGGTGCCACGTGCATGCGATAGCGGATAAGCTAGGAGCAGTGACGGCACAG
GGGAGGAGCAGTGTTCTAGAGGACACCGGATTCTCGTTCGTAAAGTGTAAGGTGACAGGGACAG
GGGTTTTGTATCTTGGGAGGGCATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACAT
GGACAACATCATCCTCCCTAGAGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGAT
						
					

						Genomic sequence
						
							GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG
ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT
ACAACTCGGCCTCGTTCGCGGTCAACTCTCCTTTCTTTGTCGCTAAGAATATCACATTCAGGGT
ATATAACTTATTAACATATACACTAGTTGTCTTAAAATTTAATTGGACGTATAAGTTTTATATC
CATCTTAGCGATCTTATATGTATGTAGAACACGACCCCAGTCCCCTTGCCCGGTGCAGTTGGTA
AACAGGCGGTAGCATTGAGGGTGTCGGCAGACAATGCCGCGTTTTTTGGGTGTAGAATGCTTGG
TGCTCAAGACACTCTCTATGACCATTTGGGAAGACATTATTACAAAGATTGTTACATTGAGGGA
TCAGTGGACTTCATCTTTGGAAATGCCCTTTCTCTTTATGAAGTAAGCATTCTCACTTAGAATA
CTATTTAAAGTTAGAACTTTTAGTTTGGTCCTTGCTCTAACAAAAGTGGTGTTTAGGGGTGCCA
CGTGCATGCGATAGCGGATAAGCTAGGAGCAGTGACGGCACAGGGGAGGAGCAGTGTTCTAGAG
GACACCGGATTCTCGTTCGTAAAGTGTAAGGTGACAGGGACAGGGGTTTTGTATCTTGGGAGGG
CATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACATGGACAACATCATCCTCCCTAG
AGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGAT
						
					

			
		

Sequences
Splice junction sequence	CATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACATGGACAACATCATCCTCCCTAG AGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGATggagaaggtgagcatacccccactgaag gcatttataacaatagaaggagaaggagcagagaagacaacagtggaatggggagacacagcac aaacccct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 154 Transcript exons: 6671815-6671938,6672589-6672618 GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCGTAC AATTGGGGAGACCCTTCCCGTGAGAT Transcript length: 260 Transcript exons: 6671815-6671963,6672508-6672618 GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT ACAACTCGGCCTCGTTCGCGGTCTTGGGAGGGCATGGGGTCCCTTCTCAAGAGTCGTTTTTGCT TACACTTACATGGACAACATCATCCTCCCTAGAGGCTGGTACAATTGGGGAGACCCTTCCCGTG AGAT Transcript length: 633 Transcript exons: 6671815-6672004,6672098-6672304,6672383-6672618 GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT ACAACTCGGCCTCGTTCGCGGTCAACTCTCCTTTCTTTGTCGCTAAGAATATCACATTCAGGAA CACGACCCCAGTCCCCTTGCCCGGTGCAGTTGGTAAACAGGCGGTAGCATTGAGGGTGTCGGCA GACAATGCCGCGTTTTTTGGGTGTAGAATGCTTGGTGCTCAAGACACTCTCTATGACCATTTGG GAAGACATTATTACAAAGATTGTTACATTGAGGGATCAGTGGACTTCATCTTTGGAAATGCCCT TTCTCTTTATGAAGGGTGCCACGTGCATGCGATAGCGGATAAGCTAGGAGCAGTGACGGCACAG GGGAGGAGCAGTGTTCTAGAGGACACCGGATTCTCGTTCGTAAAGTGTAAGGTGACAGGGACAG GGGTTTTGTATCTTGGGAGGGCATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACAT GGACAACATCATCCTCCCTAGAGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGAT
Genomic sequence	GGAGAAGGTGAGCATACCCCCACTGAAGGCATTTATAACAATAGAAGGAGAAGGAGCAGAGAAG ACAACAGTGGAATGGGGAGACACAGCACAAACCCCTGACTCAAAAGGCAACCCCATGGGCACTT ACAACTCGGCCTCGTTCGCGGTCAACTCTCCTTTCTTTGTCGCTAAGAATATCACATTCAGGGT ATATAACTTATTAACATATACACTAGTTGTCTTAAAATTTAATTGGACGTATAAGTTTTATATC CATCTTAGCGATCTTATATGTATGTAGAACACGACCCCAGTCCCCTTGCCCGGTGCAGTTGGTA AACAGGCGGTAGCATTGAGGGTGTCGGCAGACAATGCCGCGTTTTTTGGGTGTAGAATGCTTGG TGCTCAAGACACTCTCTATGACCATTTGGGAAGACATTATTACAAAGATTGTTACATTGAGGGA TCAGTGGACTTCATCTTTGGAAATGCCCTTTCTCTTTATGAAGTAAGCATTCTCACTTAGAATA CTATTTAAAGTTAGAACTTTTAGTTTGGTCCTTGCTCTAACAAAAGTGGTGTTTAGGGGTGCCA CGTGCATGCGATAGCGGATAAGCTAGGAGCAGTGACGGCACAGGGGAGGAGCAGTGTTCTAGAG GACACCGGATTCTCGTTCGTAAAGTGTAAGGTGACAGGGACAGGGGTTTTGTATCTTGGGAGGG CATGGGGTCCCTTCTCAAGAGTCGTTTTTGCTTACACTTACATGGACAACATCATCCTCCCTAG AGGCTGGTACAATTGGGGAGACCCTTCCCGTGAGAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.681093101

Conservation Information
Conserved circRNAs	NA
PMCS	0.681093101

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6671933-6672615(+)
						
						Potential amino acid sequence
						
							MGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLY
DHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKV
TGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMEKVSIPPLKAFITIEGEGAE
KTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVS
ADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTA
QGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREME
KVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNT
TPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALS
LYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMD
NIILPRGWYNWGDPSREMEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSA
SFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHY
YKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLY
LGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSRE(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	6671933-6672615(+)
Potential amino acid sequence	MGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLY DHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKV TGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMEKVSIPPLKAFITIEGEGAE KTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVS ADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTA QGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREME KVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNT TPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALS LYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMD NIILPRGWYNWGDPSREMEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSA SFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHY YKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLY LGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSRE(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Liu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT5G19730_circ_g.1

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