Plant Circular RNA Database

Detail information of Os04g0616300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os04g0616300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_025252
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 31275382-31276145  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os04g0616300						
					
						Parent gene annotation
						
							Similar to H0525G02.10 protein. (Os04t0616300-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os04g0616300_circ_ag.1 Os04g0616300_circ_ag.2 Os04g0616300_circ_ag.3 Os04g0616300_circ_ag.4 Os04g0616300_circ_ag.5 Os04g0616300_circ_ag.6 Os04g0616300_circ_ag.7 Os04g0616300_circ_ag.8 Os04g0616300_circ_ag.9 Os04g0616300_circ_ag.10 Os04g0616300_circ_igg.1 Os04g0616300_circ_igg.2 Os04g0616300_circ_igg.3 Os04g0616300_circ_ag.4 Os04g0616300_circ_ag.5 Os04g0616300_circ_ag.6 Os04g0616300_circ_ag.7 Os04g0616300_circ_igg.8 Os04g0616300_circ_ag.9 Os04g0616300_circ_ag.10 Os04g0616300_circ_igg.11 Os04g0616300_circ_ag.12 Os04g0616300_circ_igg.13 Os04g0616400_circ_g.1 Os04g0616400_circ_ag.1 Os04g0616400_circ_igg.1 Os04g0616400_circ_ag.2 Os04g0616400_circ_ag.3 Os04g0616400_circ_ag.4 Os04g0616500_circ_ag.1 Os04g0616500_circ_ag.2 Os04g0616500_circ_ag.3 Os04g0616500_circ_igg.1 Os04g0616700_circ_igg.1 Os04g0616700_circ_g.1 Os04g0616700_circ_g.2 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0616300-01:3

General Information
CircRNA Name	Os04g0616300_circ_g.1
ID in PlantcircBase	osa_circ_025252
Alias	NA
Organism	Oryza sativa
Position	chr4: 31275382-31276145 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os04g0616300
Parent gene annotation	Similar to H0525G02.10 protein. (Os04t0616300-01)
Parent gene strand	-
Alternative splicing	Os04g0616300_circ_ag.1 Os04g0616300_circ_ag.2 Os04g0616300_circ_ag.3 Os04g0616300_circ_ag.4 Os04g0616300_circ_ag.5 Os04g0616300_circ_ag.6 Os04g0616300_circ_ag.7 Os04g0616300_circ_ag.8 Os04g0616300_circ_ag.9 Os04g0616300_circ_ag.10 Os04g0616300_circ_igg.1 Os04g0616300_circ_igg.2 Os04g0616300_circ_igg.3 Os04g0616300_circ_ag.4 Os04g0616300_circ_ag.5 Os04g0616300_circ_ag.6 Os04g0616300_circ_ag.7 Os04g0616300_circ_igg.8 Os04g0616300_circ_ag.9 Os04g0616300_circ_ag.10 Os04g0616300_circ_igg.11 Os04g0616300_circ_ag.12 Os04g0616300_circ_igg.13 Os04g0616400_circ_g.1 Os04g0616400_circ_ag.1 Os04g0616400_circ_igg.1 Os04g0616400_circ_ag.2 Os04g0616400_circ_ag.3 Os04g0616400_circ_ag.4 Os04g0616500_circ_ag.1 Os04g0616500_circ_ag.2 Os04g0616500_circ_ag.3 Os04g0616500_circ_igg.1 Os04g0616700_circ_igg.1 Os04g0616700_circ_g.1 Os04g0616700_circ_g.2
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0616300-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGGATATAGTTTCTATTTCCGTTGCAAATTGCTTTTTTCCTTGATGAGATGTTTGAGATAGCTG
CTTCACTGCCACTACCCTTCCATCATTTAACTTACCccattcgaaaatgtaaatcttatcttct
tcaagtgtatcatcgtagttcggccgtccagctaaagtctccaataagaccacgccaaatgcaa
agacatca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 594
Transcript exons: 31275382-31275532,31275613-31275844,31275935-31276145
CCATTCGAAAATGTAAATCTTATCTTCTTCAAGTGTATCATCGTAGTTCGGCCGTCCAGCTAAA
GTCTCCAATAAGACCACGCCAAATGCAAAGACATCAACTTTCTCAGTCATATGACCTCTCATGG
CATACTCAGGTGCAAGATAACCGAATGTACCAGCAACTTTCGTGCTGACATGCGTCTTCTTGTC
ATCATAAAGTTTGGCTAGCCCAAAATCTGAGATCTTAGGATTGAGATTGGCGTCAAGTAAGACA
TTGCTGGCCTTGATGTCCCTGTGCACGACACGGATGCTGGACTCTTCATGAAGATAAGCCAGAC
CTCTTGCAATGCCTAAGCATATGTCAAAACGTGCTGGCCAATCTATGTTCAATTTCTCAATTCC
AAAGAGTGCTTTATCAAGGCTTCCATTTTCCATGTACTCATAAACCAGCAGTGGATTATTGCCT
TCAAGGCAGCAACCATATAACTTCACAAGATTACGGTGTTGCACTCGGGATATAGTTTCTATTT
CCGTTGCAAATTGCTTTTTTCCTTGATGAGATGTTTGAGATAGCTGCTTCACTGCCACTACCCT
TCCATCATTTAACTTACC
						
						Genomic sequence
						
							CCATTCGAAAATGTAAATCTTATCTTCTTCAAGTGTATCATCGTAGTTCGGCCGTCCAGCTAAA
GTCTCCAATAAGACCACGCCAAATGCAAAGACATCAACTTTCTCAGTCATATGACCTCTCATGG
CATACTCAGGTGCAAGATAACCGCTGGACAAAGGATACCCAAAAATACCATTAACATAAATATG
ATTGTAAACATAAAGGTGGAACAGATGGAATGTACTTACAATGTACCAGCAACTTTCGTGCTGA
CATGCGTCTTCTTGTCATCATAAAGTTTGGCTAGCCCAAAATCTGAGATCTTAGGATTGAGATT
GGCGTCAAGTAAGACATTGCTGGCCTTGATGTCCCTGTGCACGACACGGATGCTGGACTCTTCA
TGAAGATAAGCCAGACCTCTTGCAATGCCTAAGCATATGTCAAAACGTGCTGGCCAATCTATGT
TCAATTTCTCAATTCCTGCAAATGGAGGGTTTAGATTTTAGGTTACAGACTATGATAAACCTAA
ATTATAAAATCCCAATTGCATCAAACTGAAATAGCTTGTACCAAAGAGTGCTTTATCAAGGCTT
CCATTTTCCATGTACTCATAAACCAGCAGTGGATTATTGCCTTCAAGGCAGCAACCATATAACT
TCACAAGATTACGGTGTTGCACTCGGGATATAGTTTCTATTTCCGTTGCAAATTGCTTTTTTCC
TTGATGAGATGTTTGAGATAGCTGCTTCACTGCCACTACCCTTCCATCATTTAACTTACC

Sequences
Splice junction sequence	GGGATATAGTTTCTATTTCCGTTGCAAATTGCTTTTTTCCTTGATGAGATGTTTGAGATAGCTG CTTCACTGCCACTACCCTTCCATCATTTAACTTACCccattcgaaaatgtaaatcttatcttct tcaagtgtatcatcgtagttcggccgtccagctaaagtctccaataagaccacgccaaatgcaa agacatca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 594 Transcript exons: 31275382-31275532,31275613-31275844,31275935-31276145 CCATTCGAAAATGTAAATCTTATCTTCTTCAAGTGTATCATCGTAGTTCGGCCGTCCAGCTAAA GTCTCCAATAAGACCACGCCAAATGCAAAGACATCAACTTTCTCAGTCATATGACCTCTCATGG CATACTCAGGTGCAAGATAACCGAATGTACCAGCAACTTTCGTGCTGACATGCGTCTTCTTGTC ATCATAAAGTTTGGCTAGCCCAAAATCTGAGATCTTAGGATTGAGATTGGCGTCAAGTAAGACA TTGCTGGCCTTGATGTCCCTGTGCACGACACGGATGCTGGACTCTTCATGAAGATAAGCCAGAC CTCTTGCAATGCCTAAGCATATGTCAAAACGTGCTGGCCAATCTATGTTCAATTTCTCAATTCC AAAGAGTGCTTTATCAAGGCTTCCATTTTCCATGTACTCATAAACCAGCAGTGGATTATTGCCT TCAAGGCAGCAACCATATAACTTCACAAGATTACGGTGTTGCACTCGGGATATAGTTTCTATTT CCGTTGCAAATTGCTTTTTTCCTTGATGAGATGTTTGAGATAGCTGCTTCACTGCCACTACCCT TCCATCATTTAACTTACC
Genomic sequence	CCATTCGAAAATGTAAATCTTATCTTCTTCAAGTGTATCATCGTAGTTCGGCCGTCCAGCTAAA GTCTCCAATAAGACCACGCCAAATGCAAAGACATCAACTTTCTCAGTCATATGACCTCTCATGG CATACTCAGGTGCAAGATAACCGCTGGACAAAGGATACCCAAAAATACCATTAACATAAATATG ATTGTAAACATAAAGGTGGAACAGATGGAATGTACTTACAATGTACCAGCAACTTTCGTGCTGA CATGCGTCTTCTTGTCATCATAAAGTTTGGCTAGCCCAAAATCTGAGATCTTAGGATTGAGATT GGCGTCAAGTAAGACATTGCTGGCCTTGATGTCCCTGTGCACGACACGGATGCTGGACTCTTCA TGAAGATAAGCCAGACCTCTTGCAATGCCTAAGCATATGTCAAAACGTGCTGGCCAATCTATGT TCAATTTCTCAATTCCTGCAAATGGAGGGTTTAGATTTTAGGTTACAGACTATGATAAACCTAA ATTATAAAATCCCAATTGCATCAAACTGAAATAGCTTGTACCAAAGAGTGCTTTATCAAGGCTT CCATTTTCCATGTACTCATAAACCAGCAGTGGATTATTGCCTTCAAGGCAGCAACCATATAACT TCACAAGATTACGGTGTTGCACTCGGGATATAGTTTCTATTTCCGTTGCAAATTGCTTTTTTCC TTGATGAGATGTTTGAGATAGCTGCTTCACTGCCACTACCCTTCCATCATTTAACTTACC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.321070212

Conservation Information
Conserved circRNAs	NA
PMCS	0.321070212

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							31276095-31275634(+)
31275970-31275384(-)
						
						Potential amino acid sequence
						
							MFEIAASLPLPFHHLTYPIRKCKSYLLQVYHRSSAVQLKSPIRPRQMQRHQLSQSYDLSWHTQV
QDNRMYQQLSC*(+)
MENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKASNVLLDANLNPK
ISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLLETLAGRPNYDDT
LEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLVKLYGCCLEGNNP
LLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKASNVLLD
ANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLLETLAGR
PNYDDTLEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLVKLYGCC
LEGNNPLLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKA
SNVLLDANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLL
ETLAGRPNYDDTLEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLV
KLYGCCLEGNNPLLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVV
HRDIKASNVLLDANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFA
FGVVLLETLAGRPNYDDTLEEDKIYIFEW(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	31276095-31275634(+) 31275970-31275384(-)
Potential amino acid sequence	MFEIAASLPLPFHHLTYPIRKCKSYLLQVYHRSSAVQLKSPIRPRQMQRHQLSQSYDLSWHTQV QDNRMYQQLSC*(+) MENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKASNVLLDANLNPK ISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLLETLAGRPNYDDT LEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLVKLYGCCLEGNNP LLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKASNVLLD ANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLLETLAGR PNYDDTLEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLVKLYGCC LEGNNPLLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVVHRDIKA SNVLLDANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFAFGVVLL ETLAGRPNYDDTLEEDKIYIFEWGKLNDGRVVAVKQLSQTSHQGKKQFATEIETISRVQHRNLV KLYGCCLEGNNPLLVYEYMENGSLDKALFGIEKLNIDWPARFDICLGIARGLAYLHEESSIRVV HRDIKASNVLLDANLNPKISDFGLAKLYDDKKTHVSTKVAGTFGYLAPEYAMRGHMTEKVDVFA FGVVLLETLAGRPNYDDTLEEDKIYIFEW(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os04g0616300_circ_g.1

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