Plant Circular RNA Database

Detail information of Os02g0728500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0728500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_016389
					
						Alias
						Os_ciR1064
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 30319091-30319406  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, circseq_cup, find_circ
					
						Parent gene
						
							Os02g0728500						
					
						Parent gene annotation
						
							Similar to BRASSINOSTEROID INSENSITIVE 1-associated receptor kin
ase 1. (Os02t0728500-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0728500_circ_g.1 						
					
						Support reads
						22/8/11
					
						Tissues
						root/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0728500-01:2

General Information
CircRNA Name	Os02g0728500_circ_g.2
ID in PlantcircBase	osa_circ_016389
Alias	Os_ciR1064
Organism	Oryza sativa
Position	chr2: 30319091-30319406 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, circseq_cup, find_circ
Parent gene	Os02g0728500
Parent gene annotation	Similar to BRASSINOSTEROID INSENSITIVE 1-associated receptor kin ase 1. (Os02t0728500-01)
Parent gene strand	-
Alternative splicing	Os02g0728500_circ_g.1
Support reads	22/8/11
Tissues	root/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0728500-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GAATCTTTCTGGCCTGCTCT
 R: AGGATCCACGGAGTCTTGG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GAATCTTTCTGGCCTGCTCT R: AGGATCCACGGAGTCTTGG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACCATGGTCCAGCTGCAAGGATCCACGGAGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGAT
CCTTGAGGGAAGTCTTGATCATCATGAGAGCTTGCAactatctcaagattggtcaagttgccta
tgcttgcggagagcaggccagaaagattctggcttggagcctccctatcaaattcagacaagca
aacaaata

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 200
Transcript exons: 30319091-30319156,30319273-30319406
ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG
GACAGGCCAGTGACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATCCACGG
AGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATCATGAG
AGCTTGCA
Transcript length: 205
Transcript exons: 30319091-30319162,30319274-30319406
ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG
GAGCCTCCAGGCCAGTGACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATC
CACGGAGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATC
ATGAGAGCTTGCA
						
						Genomic sequence
						
							ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG
GAGCCTCCCTATCAAATTCAGACAAGCAAACAAATAATGGTAGGTCAGCAAGTCAAAGGAAACT
TGTCAAGATATGTTGGGGTTGAGTAAAAATATGATACTCTAGATGGCTTGCTTACAGGCCAGTG
ACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATCCACGGAGTCTTGGTCCC
AGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATCATGAGAGCTTGCA

Sequences
Splice junction sequence	ACCATGGTCCAGCTGCAAGGATCCACGGAGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGAT CCTTGAGGGAAGTCTTGATCATCATGAGAGCTTGCAactatctcaagattggtcaagttgccta tgcttgcggagagcaggccagaaagattctggcttggagcctccctatcaaattcagacaagca aacaaata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 200 Transcript exons: 30319091-30319156,30319273-30319406 ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG GACAGGCCAGTGACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATCCACGG AGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATCATGAG AGCTTGCA Transcript length: 205 Transcript exons: 30319091-30319162,30319274-30319406 ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG GAGCCTCCAGGCCAGTGACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATC CACGGAGTCTTGGTCCCAGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATC ATGAGAGCTTGCA
Genomic sequence	ACTATCTCAAGATTGGTCAAGTTGCCTATGCTTGCGGAGAGCAGGCCAGAAAGATTCTGGCTTG GAGCCTCCCTATCAAATTCAGACAAGCAAACAAATAATGGTAGGTCAGCAAGTCAAAGGAAACT TGTCAAGATATGTTGGGGTTGAGTAAAAATATGATACTCTAGATGGCTTGCTTACAGGCCAGTG ACAAGGTTCTCAGGTGAGCAAGTGACCATGGTCCAGCTGCAAGGATCCACGGAGTCTTGGTCCC AGTTCTTGAGCACACCGTGAGGATCCTTGAGGGAAGTCTTGATCATCATGAGAGCTTGCA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_012240 						
					
						PMCS
						0.36106192

Conservation Information
Conserved circRNAs	osi_circ_012240
PMCS	0.36106192

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							30319395-30319296(+)
30319397-30319294(-)
						
						Potential amino acid sequence
						
							MRACNYLKIGQVAYACGEQARKILAWSLQASDKVLR*(+)
MMIKTSLKDPHGVLKNWDQDSVDPCSWTMVTCSPENLVTGLEAPSQNLSGLLSASIGNLTNLEI
VASSHDDQDFPQGSSRCAQELGPRLRGSLQLDHGHLLT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	30319395-30319296(+) 30319397-30319294(-)
Potential amino acid sequence	MRACNYLKIGQVAYACGEQARKILAWSLQASDKVLR(+) MMIKTSLKDPHGVLKNWDQDSVDPCSWTMVTCSPENLVTGLEAPSQNLSGLLSASIGNLTNLEI VASSHDDQDFPQGSSRCAQELGPRLRGSLQLDHGHLLT(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Ye et al., 2016;Chu et al., 2017

circRNA

Detail information of Os02g0728500_circ_g.2

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