Plant Circular RNA Database

Detail information of Os07g0164300_circ_g.7

			
						 General Information
					
						CircRNA Name
						Os07g0164300_circ_g.7
					
						ID in PlantcircBase
						osa_circ_032891
					
						Alias
						Os_ciR4344
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 3448114-3449205  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os07g0164300						
					
						Parent gene annotation
						
							Similar to syntaxin 132. (Os07t0164300-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os07g0164300_circ_g.2 Os07g0164300_circ_g.3 Os07g0164300_circ_g.4 Os07g0164300_circ_g.5 Os07g0164300_circ_g.6 Os07g0164300_circ_g.8 Os07g0164300_circ_g.9 						
					
						Support reads
						6/3
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0164300-01:5

General Information
CircRNA Name	Os07g0164300_circ_g.7
ID in PlantcircBase	osa_circ_032891
Alias	Os_ciR4344
Organism	Oryza sativa
Position	chr7: 3448114-3449205 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os07g0164300
Parent gene annotation	Similar to syntaxin 132. (Os07t0164300-01)
Parent gene strand	-
Alternative splicing	Os07g0164300_circ_g.2 Os07g0164300_circ_g.3 Os07g0164300_circ_g.4 Os07g0164300_circ_g.5 Os07g0164300_circ_g.6 Os07g0164300_circ_g.8 Os07g0164300_circ_g.9
Support reads	6/3
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0164300-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTTCAAGAAACCTTTTAGATTGTCTGAAGGATCAGCTTGCATTCCCATTTCAATGTCCCCATC
TCTTGAAGAGCCTCCCCGAGGGAGCTCAAACGAATCccagtagtctgttctcttgatcgatcta
ccgcagatcctttcccacatccaggcttttgtctgtttgataagttctgtttcacatggcagcg
aacagtga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 371
Transcript exons: 3448114-3448187,3448535-3448623,3448726-3448774,3448938-
3448994,3449104-3449205
CCAGTAGTCTGTTCTCTTGATCGATCTACCGCAGATCCTTTCCCACATCCAGGCTTTTGTCTGT
TTGATAAGTTATCTTTTTCCAATTCATCAACTTTTGTCTTTGCCATGCGAGCGATTTTCCCCAC
TTCATCAATATCTTTCTCCATCCTCTGTTTAATTGCTTTCATGTCCCTTGCTTTTGTAACCGCT
TTAGATTCCTCATTTGCAGTCTGAAGCTTATGCAAGAGATTCGTCAGCTTAGCAATTAGGCTTT
CAATTGCGTCAACCTTTTTCAAGAAACCTTTTAGATTGTCTGAAGGATCAGCTTGCATTCCCAT
TTCAATGTCCCCATCTCTTGAAGAGCCTCCCCGAGGGAGCTCAAACGAATC
						
					

						Genomic sequence
						
							CCAGTAGTCTGTTCTCTTGATCGATCTACCGCAGATCCTTTCCCACATCCAGGCTTTTGTCTGT
TTGATAAGTTCTGTTTCACATGGCAGCGAACAGTGAGAGACAACTCAATGTTGCTATTGACAAA
TGACACTAGCACGTGAGATGGCTTAGTGAAAAGTATAGCAAATCCTACAATTTGTTTTAGGGGC
ACTACTAAATCATAACCTTGTGATACAAGTTGCAGAGAATGCTCTTATGAAAATATAATGCATA
CGTTGGATAGACATGGTCAATTTGTTACTTACTGCAGTGCTATATGACTGTTGATGATTTATAT
ATATTGTATATAGAGTGCAGACACATGAAATAAAAACAGAATATTACGCAGATATGGCAAATTA
ATACAATGCCATTAGCTAGCAGGGCCAAGTGACATACATCTTTTTCCAATTCATCAACTTTTGT
CTTTGCCATGCGAGCGATTTTCCCCACTTCATCAATATCTTTCTCCATCCTCTGTTTAATTGCT
ATGGAAAAATAATTGTTGCATCAACAATAGGTAAAGTGGGTTATCAAAAGTGTACAAGAACTTT
TTAGACAAGGTTAAATATACTAAACTAAGTACTCACCTTTCATGTCCCTTGCTTTTGTAACCGC
TTTAGATTCCTCATTTGCAGTCTACAAGCAAAAGTAAGGTGTATATGAAAATTCAAGATGCTAA
ATGAATTCATCAAAACTTAGACACAACATTATATTTCTGGTACTACGAAAATCAATATCATCAG
AACAAGACAAGGACCAGAAACAGAAAGAAATGAATGGAAGTGTGAGATAAATAAACCTGAAGCT
TATGCAAGAGATTCGTCAGCTTAGCAATTAGGCTTTCAATTGCGTCAACCTGATGTATCAAAGC
AAGATGTCAATAATATGAAGTGTCTAGTAAGTGGGTAATATAATACTGCTGCAAAATGCAAGAT
GCACACACCAAAATGCTTTGTAAACAGCACCTTTTTCAAGAAACCTTTTAGATTGTCTGAAGGA
TCAGCTTGCATTCCCATTTCAATGTCCCCATCTCTTGAAGAGCCTCCCCGAGGGAGCTCAAACG
AATC
						
					

			
		

Sequences
Splice junction sequence	TTTTCAAGAAACCTTTTAGATTGTCTGAAGGATCAGCTTGCATTCCCATTTCAATGTCCCCATC TCTTGAAGAGCCTCCCCGAGGGAGCTCAAACGAATCccagtagtctgttctcttgatcgatcta ccgcagatcctttcccacatccaggcttttgtctgtttgataagttctgtttcacatggcagcg aacagtga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 371 Transcript exons: 3448114-3448187,3448535-3448623,3448726-3448774,3448938- 3448994,3449104-3449205 CCAGTAGTCTGTTCTCTTGATCGATCTACCGCAGATCCTTTCCCACATCCAGGCTTTTGTCTGT TTGATAAGTTATCTTTTTCCAATTCATCAACTTTTGTCTTTGCCATGCGAGCGATTTTCCCCAC TTCATCAATATCTTTCTCCATCCTCTGTTTAATTGCTTTCATGTCCCTTGCTTTTGTAACCGCT TTAGATTCCTCATTTGCAGTCTGAAGCTTATGCAAGAGATTCGTCAGCTTAGCAATTAGGCTTT CAATTGCGTCAACCTTTTTCAAGAAACCTTTTAGATTGTCTGAAGGATCAGCTTGCATTCCCAT TTCAATGTCCCCATCTCTTGAAGAGCCTCCCCGAGGGAGCTCAAACGAATC
Genomic sequence	CCAGTAGTCTGTTCTCTTGATCGATCTACCGCAGATCCTTTCCCACATCCAGGCTTTTGTCTGT TTGATAAGTTCTGTTTCACATGGCAGCGAACAGTGAGAGACAACTCAATGTTGCTATTGACAAA TGACACTAGCACGTGAGATGGCTTAGTGAAAAGTATAGCAAATCCTACAATTTGTTTTAGGGGC ACTACTAAATCATAACCTTGTGATACAAGTTGCAGAGAATGCTCTTATGAAAATATAATGCATA CGTTGGATAGACATGGTCAATTTGTTACTTACTGCAGTGCTATATGACTGTTGATGATTTATAT ATATTGTATATAGAGTGCAGACACATGAAATAAAAACAGAATATTACGCAGATATGGCAAATTA ATACAATGCCATTAGCTAGCAGGGCCAAGTGACATACATCTTTTTCCAATTCATCAACTTTTGT CTTTGCCATGCGAGCGATTTTCCCCACTTCATCAATATCTTTCTCCATCCTCTGTTTAATTGCT ATGGAAAAATAATTGTTGCATCAACAATAGGTAAAGTGGGTTATCAAAAGTGTACAAGAACTTT TTAGACAAGGTTAAATATACTAAACTAAGTACTCACCTTTCATGTCCCTTGCTTTTGTAACCGC TTTAGATTCCTCATTTGCAGTCTACAAGCAAAAGTAAGGTGTATATGAAAATTCAAGATGCTAA ATGAATTCATCAAAACTTAGACACAACATTATATTTCTGGTACTACGAAAATCAATATCATCAG AACAAGACAAGGACCAGAAACAGAAAGAAATGAATGGAAGTGTGAGATAAATAAACCTGAAGCT TATGCAAGAGATTCGTCAGCTTAGCAATTAGGCTTTCAATTGCGTCAACCTGATGTATCAAAGC AAGATGTCAATAATATGAAGTGTCTAGTAAGTGGGTAATATAATACTGCTGCAAAATGCAAGAT GCACACACCAAAATGCTTTGTAAACAGCACCTTTTTCAAGAAACCTTTTAGATTGTCTGAAGGA TCAGCTTGCATTCCCATTTCAATGTCCCCATCTCTTGAAGAGCCTCCCCGAGGGAGCTCAAACG AATC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.235014744

Conservation Information
Conserved circRNAs	NA
PMCS	0.235014744

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3449156-3449123(-)
3448163-3449123(-)
						
						Potential amino acid sequence
						
							MGMQADPSDNLKGFLKKVDAIESLIAKLTNLLHKLQTANEESKAVTKARDMKAIKQRMEKDIDE
VGKIARMAKTKVDELEKDNLSNRQKPGCGKGSAVDRSREQTTGIRLSSLGEALQEMGTLKWECK
LILQTI*(-)
MWERICGRSIKRTDYWDSFELPRGGSSRDGDIEMGMQADPSDNLKGFLKKVDAIESLIAKLTNL
LHKLQTANEESKAVTKARDMKAIKQRMEKDIDEVGKIARMAKTKVDELEKDNLSNRQKPGCGKG
SAVDRSREQTTGIRLSSLGEALQEMGTLKWECKLILQTI*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3449156-3449123(-) 3448163-3449123(-)
Potential amino acid sequence	MGMQADPSDNLKGFLKKVDAIESLIAKLTNLLHKLQTANEESKAVTKARDMKAIKQRMEKDIDE VGKIARMAKTKVDELEKDNLSNRQKPGCGKGSAVDRSREQTTGIRLSSLGEALQEMGTLKWECK LILQTI(-) MWERICGRSIKRTDYWDSFELPRGGSSRDGDIEMGMQADPSDNLKGFLKKVDAIESLIAKLTNL LHKLQTANEESKAVTKARDMKAIKQRMEKDIDEVGKIARMAKTKVDELEKDNLSNRQKPGCGKG SAVDRSREQTTGIRLSSLGEALQEMGTLKWECKLILQTI(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os07g0164300_circ_g.7

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