Plant Circular RNA Database

Detail information of Os02g0656500_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os02g0656500_circ_g.5
					
						ID in PlantcircBase
						osa_circ_015828
					
						Alias
						Os02circ20414
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 26522136-26522278  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os02g0656500						
					
						Parent gene annotation
						
							DnaJ-like protein. (Os02t0656500-01);Similar to DnaJ-like protei
n. (Os02t0656500-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0656500_circ_g.1 Os02g0656500_circ_g.2 Os02g0656500_circ_g.3 Os02g0656500_circ_g.4 Os02g0656500_circ_g.6 						
					
						Support reads
						3
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0656500-02:1
Os02t0656500-01:1

General Information
CircRNA Name	Os02g0656500_circ_g.5
ID in PlantcircBase	osa_circ_015828
Alias	Os02circ20414
Organism	Oryza sativa
Position	chr2: 26522136-26522278 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os02g0656500
Parent gene annotation	DnaJ-like protein. (Os02t0656500-01);Similar to DnaJ-like protei n. (Os02t0656500-02)
Parent gene strand	+
Alternative splicing	Os02g0656500_circ_g.1 Os02g0656500_circ_g.2 Os02g0656500_circ_g.3 Os02g0656500_circ_g.4 Os02g0656500_circ_g.6
Support reads	3
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0656500-02:1 Os02t0656500-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGTGAAGCTGA
TGAAGCTgtgagatgataagtgacaaggataaatgcccgagttgtaagggaaacaaagtagtcc
agcagaagaaggtct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 143
Transcript exons: 26522136-26522278
GTGAGATGATAAGTGACAAGGATAAATGCCCGAGTTGTAAGGGAAACAAAGTAGTCCAGCAGAA
GAAGGTCTTGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGT
GAAGCTGATGAAGCT
						
						Genomic sequence
						
							GTGAGATGATAAGTGACAAGGATAAATGCCCGAGTTGTAAGGGAAACAAAGTAGTCCAGCAGAA
GAAGGTCTTGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGT
GAAGCTGATGAAGCT

Sequences
Splice junction sequence	TGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGTGAAGCTGA TGAAGCTgtgagatgataagtgacaaggataaatgcccgagttgtaagggaaacaaagtagtcc agcagaagaaggtct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 143 Transcript exons: 26522136-26522278 GTGAGATGATAAGTGACAAGGATAAATGCCCGAGTTGTAAGGGAAACAAAGTAGTCCAGCAGAA GAAGGTCTTGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGT GAAGCTGATGAAGCT
Genomic sequence	GTGAGATGATAAGTGACAAGGATAAATGCCCGAGTTGTAAGGGAAACAAAGTAGTCCAGCAGAA GAAGGTCTTGGAGGTTCATGTTGAGAAGGGAATGCAACATGGCCAAAAGATTGTATTCCAGGGT GAAGCTGATGAAGCT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.425637995

Conservation Information
Conserved circRNAs	NA
PMCS	0.425637995

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26522218-26522136(+)
26522142-26522141(+)
26522241-26522217(-)
						
						Potential amino acid sequence
						
							MLRRECNMAKRLYSRVKLMKL*(+)
MISDKDKCPSCKGNKVVQQKKVLEVHVEKGMQHGQKIVFQGEADEAVR*(+)
MLHSLLNMNLQDLLLLDYFVSLTTRAFILVTYHLTASSASPWNTIFWPCCIPFST*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26522218-26522136(+) 26522142-26522141(+) 26522241-26522217(-)
Potential amino acid sequence	MLRRECNMAKRLYSRVKLMKL(+) MISDKDKCPSCKGNKVVQQKKVLEVHVEKGMQHGQKIVFQGEADEAVR(+) MLHSLLNMNLQDLLLLDYFVSLTTRAFILVTYHLTASSASPWNTIFWPCCIPFST*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os02g0656500_circ_g.5

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