Plant Circular RNA Database

Detail information of AT1G65230_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT1G65230_circ_g.5
					
						ID in PlantcircBase
						ath_circ_008762
					
						Alias
						At_ciR3856
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr1: 24230222-24230569  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT1G65230						
					
						Parent gene annotation
						
							Putative uncharacterized protein At1g65230
							
						Parent gene strand
						+
					
						Alternative splicing
						
							1_circ_ag.3 AT1G65080_circ_g.1 AT1G65130_circ_g.1 AT1G65220_circ_g.1 AT1G65220_circ_g.2 AT1G65220_circ_g.3 AT1G65220_circ_g.4 AT1G65220_circ_g.5 AT1G65220_circ_g.6 AT1G65230_circ_g.1 AT1G65230_circ_g.2 AT1G65230_circ_g.3 AT1G65230_circ_g.4 AT1G65260_circ_g.1 AT1G65260_circ_g.2 AT1G65260_circ_g.3 AT1G65260_circ_g.4 AT1G65260_circ_g.5 AT1G65260_circ_g.6 AT1G65260_circ_g.7 AT1G65260_circ_g.8 AT1G65260_circ_g.9 AT1G65260_circ_g.10 AT1G65260_circ_g.11 AT1G65260_circ_g.12 AT1G65260_circ_g.13 AT1G65260_circ_g.14 AT1G65260_circ_g.15 AT1G65270_circ_g.1 AT1G65270_circ_g.2 AT1G65270_circ_g.3 AT1G65270_circ_g.4 AT1G65270_circ_g.5 AT1G65270_circ_g.6 AT1G65280_circ_g.1 AT1G65280_circ_g.2 AT1G65280_circ_g.3 AT1G65290_circ_g.1 AT1G65290_circ_g.2 AT1G65295_circ_g.1 AT1G65320_circ_g.1 AT1G65350_circ_g.1 AT1G65370_circ_g.1 AT1G65370_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT1G65230.1:1

General Information
CircRNA Name	AT1G65230_circ_g.5
ID in PlantcircBase	ath_circ_008762
Alias	At_ciR3856
Organism	Arabidpsis thaliana
Position	chr1: 24230222-24230569 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ
Parent gene	AT1G65230
Parent gene annotation	Putative uncharacterized protein At1g65230
Parent gene strand	+
Alternative splicing	1_circ_ag.3 AT1G65080_circ_g.1 AT1G65130_circ_g.1 AT1G65220_circ_g.1 AT1G65220_circ_g.2 AT1G65220_circ_g.3 AT1G65220_circ_g.4 AT1G65220_circ_g.5 AT1G65220_circ_g.6 AT1G65230_circ_g.1 AT1G65230_circ_g.2 AT1G65230_circ_g.3 AT1G65230_circ_g.4 AT1G65260_circ_g.1 AT1G65260_circ_g.2 AT1G65260_circ_g.3 AT1G65260_circ_g.4 AT1G65260_circ_g.5 AT1G65260_circ_g.6 AT1G65260_circ_g.7 AT1G65260_circ_g.8 AT1G65260_circ_g.9 AT1G65260_circ_g.10 AT1G65260_circ_g.11 AT1G65260_circ_g.12 AT1G65260_circ_g.13 AT1G65260_circ_g.14 AT1G65260_circ_g.15 AT1G65270_circ_g.1 AT1G65270_circ_g.2 AT1G65270_circ_g.3 AT1G65270_circ_g.4 AT1G65270_circ_g.5 AT1G65270_circ_g.6 AT1G65280_circ_g.1 AT1G65280_circ_g.2 AT1G65280_circ_g.3 AT1G65290_circ_g.1 AT1G65290_circ_g.2 AT1G65295_circ_g.1 AT1G65320_circ_g.1 AT1G65350_circ_g.1 AT1G65370_circ_g.1 AT1G65370_circ_g.2
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT1G65230.1:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGCAAAAGATGTCGCCGATGTCACAAAAGACTTGACCGCCAATTTGACAACTCGAAAAGAGGAC
ACAGATATATATCGCGATCCAACCGATCCAAATAAGacggtacaagagatggtaatgttatcga
ccagcgtcttgcgaattaaatggacagtgaaaggaaaaccaaaatctattcttgctgctgtaag
tggagatt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							ACGGTACAAGAGATGGTAATGTTATCGACCAGCGTCTTGCGAATTAAATGGACAGTGAAAGGAA
AACCAAAATCTATTCTTGCTGCTGTAAGTGGAGATTTGATAGTAAAAGTCAAATCTGAATTTAC
TCTTAACCAGATTAGTGGGCAAGTGTTTGAGCATGAAGAGTCTTGGGACTTATCTTCATCATCC
CCTATTGCTCAAGCCTATTTCTGGACATCCCGCCGCCTCTTTGCTGCTTCTGAATCCGCAAAAG
ATGTCGCCGATGTCACAAAAGACTTGACCGCCAATTTGACAACTCGAAAAGAGGACACAGATAT
ATATCGCGATCCAACCGATCCAAATAAG

Sequences
Splice junction sequence	CGCAAAAGATGTCGCCGATGTCACAAAAGACTTGACCGCCAATTTGACAACTCGAAAAGAGGAC ACAGATATATATCGCGATCCAACCGATCCAAATAAGacggtacaagagatggtaatgttatcga ccagcgtcttgcgaattaaatggacagtgaaaggaaaaccaaaatctattcttgctgctgtaag tggagatt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	ACGGTACAAGAGATGGTAATGTTATCGACCAGCGTCTTGCGAATTAAATGGACAGTGAAAGGAA AACCAAAATCTATTCTTGCTGCTGTAAGTGGAGATTTGATAGTAAAAGTCAAATCTGAATTTAC TCTTAACCAGATTAGTGGGCAAGTGTTTGAGCATGAAGAGTCTTGGGACTTATCTTCATCATCC CCTATTGCTCAAGCCTATTTCTGGACATCCCGCCGCCTCTTTGCTGCTTCTGAATCCGCAAAAG ATGTCGCCGATGTCACAAAAGACTTGACCGCCAATTTGACAACTCGAAAAGAGGACACAGATAT ATATCGCGATCCAACCGATCCAAATAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.187510919

Conservation Information
Conserved circRNAs	NA
PMCS	0.187510919

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24230234-24230566(+)
						
						Potential amino acid sequence
						
							MVMLSTSVLRIKWTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQ
AYFWTSRRLFAASESAKDVADVTKDLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIK
WTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAA
SESAKDVADVTKDLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIKWTVKGKPKSILA
AVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAASESAKDVADVTK
DLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIKWTVKGKPKSILAAVSGDLIVKVKS
EFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAASESAKDVADVTKDLTANLTTRKED
TDIYRDPTDPNK(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24230234-24230566(+)
Potential amino acid sequence	MVMLSTSVLRIKWTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQ AYFWTSRRLFAASESAKDVADVTKDLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIK WTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAA SESAKDVADVTKDLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIKWTVKGKPKSILA AVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAASESAKDVADVTK DLTANLTTRKEDTDIYRDPTDPNKTVQEMVMLSTSVLRIKWTVKGKPKSILAAVSGDLIVKVKS EFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRRLFAASESAKDVADVTKDLTANLTTRKED TDIYRDPTDPNK(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of AT1G65230_circ_g.5

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