Plant Circular RNA Database

Detail information of Os02g0194700_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os02g0194700_circ_g.6
					
						ID in PlantcircBase
						osa_circ_013618
					
						Alias
						Os02circ07060
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 5280637-5281014  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl
					
						Parent gene
						
							Os02g0194700						
					
						Parent gene annotation
						
							Similar to Lipoxygenase 2.3, chloroplast precursor (EC 1.13.11.1
2) (LOX2:Hv:3). (Os02t0194700-01);Similar to Lipoxygenase. (Os02
t0194700-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0194700_circ_g.4 Os02g0194700_circ_g.5 						
					
						Support reads
						15/2
					
						Tissues
						leaf/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0194700-02:1
Os02t0194700-01:1

General Information
CircRNA Name	Os02g0194700_circ_g.6
ID in PlantcircBase	osa_circ_013618
Alias	Os02circ07060
Organism	Oryza sativa
Position	chr2: 5280637-5281014 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl
Parent gene	Os02g0194700
Parent gene annotation	Similar to Lipoxygenase 2.3, chloroplast precursor (EC 1.13.11.1 2) (LOX2:Hv:3). (Os02t0194700-01);Similar to Lipoxygenase. (Os02 t0194700-02)
Parent gene strand	-
Alternative splicing	Os02g0194700_circ_g.4 Os02g0194700_circ_g.5
Support reads	15/2
Tissues	leaf/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0194700-02:1 Os02t0194700-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CTTCACGTCCGAGAACTGCT
 R: CACCTCCACCAACATCGTC
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CTTCACGTCCGAGAACTGCT R: CACCTCCACCAACATCGTC PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCA
CGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGATgctcgaacatctcggggacgtcgaagcg
gaggacgtggtcggtggtgtcctcgatcatgcggacgaggcgggggatgacgccgccgacgatg
ttggtgga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 151
Transcript exons: 5280637-5280665,5280893-5281014
GCTCGAACATCTCGGGGACGTCGAAGCGGCGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCG
TCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACACCGGCCC
CCTCCTCTTCTCCGTCCGCGGAT
Transcript length: 158
Transcript exons: 5280637-5280681,5280902-5281014
GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGCGCGTGCAGCCCCGACCTC
AGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACA
CCGGCCCCCTCCTCTTCTCCGTCCGCGGAT
Transcript length: 184
Transcript exons: 5280637-5280705,5280900-5281014
GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC
GAGGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGA
GAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT
Transcript length: 221
Transcript exons: 5280637-5280754,5280912-5281014
GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC
GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCCCCGACCTCA
GCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACAC
CGGCCCCCTCCTCTTCTCCGTCCGCGGAT
Transcript length: 378
Transcript exons: 5280637-5281014
GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC
GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCGGCGCCGCCG
GCCGTGGCAGTGGCGGCGCCGGCGGCGGCGAGCTGCGCCGGCAGCGGGATGCCGACGCTGTAGA
GGCCGTCGATGGCCGGGAAGTGCGGGAACCTCAGCTCCTGGTTCGCCAGCAGCGGCTCCAGCGC
CGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCC
GAGAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT
						
					

						Genomic sequence
						
							GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC
GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCGGCGCCGCCG
GCCGTGGCAGTGGCGGCGCCGGCGGCGGCGAGCTGCGCCGGCAGCGGGATGCCGACGCTGTAGA
GGCCGTCGATGGCCGGGAAGTGCGGGAACCTCAGCTCCTGGTTCGCCAGCAGCGGCTCCAGCGC
CGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCC
GAGAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT
						
					

			
		

Sequences
Splice junction sequence	GACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCA CGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGATgctcgaacatctcggggacgtcgaagcg gaggacgtggtcggtggtgtcctcgatcatgcggacgaggcgggggatgacgccgccgacgatg ttggtgga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 151 Transcript exons: 5280637-5280665,5280893-5281014 GCTCGAACATCTCGGGGACGTCGAAGCGGCGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCG TCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACACCGGCCC CCTCCTCTTCTCCGTCCGCGGAT Transcript length: 158 Transcript exons: 5280637-5280681,5280902-5281014 GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGCGCGTGCAGCCCCGACCTC AGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACA CCGGCCCCCTCCTCTTCTCCGTCCGCGGAT Transcript length: 184 Transcript exons: 5280637-5280705,5280900-5281014 GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC GAGGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGA GAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT Transcript length: 221 Transcript exons: 5280637-5280754,5280912-5281014 GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCCCCGACCTCA GCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCCGAGAACTGCTCGTCCCGAGGCACGTACAC CGGCCCCCTCCTCTTCTCCGTCCGCGGAT Transcript length: 378 Transcript exons: 5280637-5281014 GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCGGCGCCGCCG GCCGTGGCAGTGGCGGCGCCGGCGGCGGCGAGCTGCGCCGGCAGCGGGATGCCGACGCTGTAGA GGCCGTCGATGGCCGGGAAGTGCGGGAACCTCAGCTCCTGGTTCGCCAGCAGCGGCTCCAGCGC CGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCC GAGAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT
Genomic sequence	GCTCGAACATCTCGGGGACGTCGAAGCGGAGGACGTGGTCGGTGGTGTCCTCGATCATGCGGAC GAGGCGGGGGATGACGCCGCCGACGATGTTGGTGGAGGTGGAGGAGGAGGCCGCGGCGCCGCCG GCCGTGGCAGTGGCGGCGCCGGCGGCGGCGAGCTGCGCCGGCAGCGGGATGCCGACGCTGTAGA GGCCGTCGATGGCCGGGAAGTGCGGGAACCTCAGCTCCTGGTTCGCCAGCAGCGGCTCCAGCGC CGGCAGCATCGCGTGCAGCCCCGACCTCAGCGTCGTCGCGCTGAACGTCATCCCCTTCACGTCC GAGAACTGCTCGTCCCGAGGCACGTACACCGGCCCCCTCCTCTTCTCCGTCCGCGGAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_003721* osi_circ_012631 						
					
						PMCS
						0.415084083

Conservation Information
Conserved circRNAs	osi_circ_003721* osi_circ_012631
PMCS	0.415084083

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5281014-5281011(+)
5280838-5280824(+)
5280945-5280639(-)
						
						Potential amino acid sequence
						
							MLEHLGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAV
EAVDGREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPRML
EHLGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAVEA
VDGREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPRMLEH
LGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAVEAVD
GREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPR(+)
MAGKCGNLSSWFASSGSSAGSIACSPDLSVVALNVIPFTSENCSSRGTYTGPLLFSVRGCSNIS
GTSKRRTWSVVSSIMRTRRGMTPPTMLVEVEEEAAAPPAVAVAAPAAASCAGSGMPTL*(+)
MTFSATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAA
ASSSTSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMT
FSATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAAS
SSTSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMTFS
ATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAASSS
TSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMTFSAT
TLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAASSSTS
TNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFE(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5281014-5281011(+) 5280838-5280824(+) 5280945-5280639(-)
Potential amino acid sequence	MLEHLGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAV EAVDGREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPRML EHLGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAVEA VDGREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPRMLEH LGDVEAEDVVGGVLDHADEAGDDAADDVGGGGGGGRGAAGRGSGGAGGGELRRQRDADAVEAVD GREVREPQLLVRQQRLQRRQHRVQPRPQRRRAERHPLHVRELLVPRHVHRPPPLLRPR(+) MAGKCGNLSSWFASSGSSAGSIACSPDLSVVALNVIPFTSENCSSRGTYTGPLLFSVRGCSNIS GTSKRRTWSVVSSIMRTRRGMTPPTMLVEVEEEAAAPPAVAVAAPAAASCAGSGMPTL*(+) MTFSATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAA ASSSTSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMT FSATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAAS SSTSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMTFS ATTLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAASSS TSTNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFEHPRTEKRRGPVYVPRDEQFSDVKGMTFSAT TLRSGLHAMLPALEPLLANQELRFPHFPAIDGLYSVGIPLPAQLAAAGAATATAGGAAASSSTS TNIVGGVIPRLVRMIEDTTDHVLRFDVPEMFE(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0194700_circ_g.6

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