Plant Circular RNA Database

Detail information of Os06g0563250_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os06g0563250_circ_g.2
					
						ID in PlantcircBase
						osa_circ_031174
					
						Alias
						Os_ciR1444
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 21656567-21658786  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os06g0563250						
					
						Parent gene annotation
						
							Hypothetical gene. (Os06t0563250-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0563250_circ_g.1 Os06g0563250_circ_g.3 Os06g0563250_circ_g.4 Os06g0563250_circ_g.5 Os06g0563250_circ_g.6 Os06g0563250_circ_g.7 Os06g0563250_circ_g.8 Os06g0563250_circ_g.9 Os06g0563250_circ_g.10 Os06g0563250_circ_g.11 Os06g0563250_circ_g.12 Os06g0563250_circ_g.13 Os06g0563300_circ_g.1 						
					
						Support reads
						12/3
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0563250-01:6

General Information
CircRNA Name	Os06g0563250_circ_g.2
ID in PlantcircBase	osa_circ_031174
Alias	Os_ciR1444
Organism	Oryza sativa
Position	chr6: 21656567-21658786 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os06g0563250
Parent gene annotation	Hypothetical gene. (Os06t0563250-01)
Parent gene strand	-
Alternative splicing	Os06g0563250_circ_g.1 Os06g0563250_circ_g.3 Os06g0563250_circ_g.4 Os06g0563250_circ_g.5 Os06g0563250_circ_g.6 Os06g0563250_circ_g.7 Os06g0563250_circ_g.8 Os06g0563250_circ_g.9 Os06g0563250_circ_g.10 Os06g0563250_circ_g.11 Os06g0563250_circ_g.12 Os06g0563250_circ_g.13 Os06g0563300_circ_g.1
Support reads	12/3
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0563250-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCAGCAGATGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTTCAATATTG
TTGACGTGAAGCCTCCAAACATGGAAGACCTAACGGttgacatcatctctgcaattgagtttga
taaatctggtgatcatcttgccaccggagacagaggaggacgtgttgttttatttgaaaggaca
gatgctcg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 732
Transcript exons: 21656567-21656670,21656744-21656839,21657084-21657203,21
657869-21658069,21658212-21658303,21658668-21658786
TTGACATCATCTCTGCAATTGAGTTTGATAAATCTGGTGATCATCTTGCCACCGGAGACAGAGG
AGGACGTGTTGTTTTATTTGAAAGGACAGATGCTCGGGATAATGCCAGTCGGAGAGAAATGGAG
AGACAAGATGCTCCGATTACTAGACACCCAGAGTTTCGCTACAAAAGTGAATTTCAGAGTCATG
AACCTGAGTTTGATTACCTCAAAAGTTTGGAAATAGAGGAGAAGATCAACAAGATCAGGTGGTG
CCAGACAGCCAATAATTCACTCTCCCTTCTGTCTACAAATGATAAAACAATAAAATACTGGAAG
GTGCAAGAGAAGAAAGTAAAACAAGTTTCAGTTATGAATTTGGATTCACGGAGTGTAGGGACTG
GTACCTCTTCTAGTGCAAGTACTAGTAGTTCTAGGGGTCTTCTTCCAAATGGTGGATGTTCAGA
CAAGTCCAGTTTCTTGAACAGTGACATTTTGTTTCCACCTGGAGGCTACCCGTCATTACGTTTG
CCTGTGGTAGTTGCAAGTCAAGATGTGAACCTTGTTGCTAGATGTCGACGTGTATATGCACATG
CTCATGATTACCATATTAACTCTATATCAACCAATAGTGATGGCGAGACATACATATCAGCAGA
TGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTTCAATATTGTTGACGTG
AAGCCTCCAAACATGGAAGACCTAACGG
						
					

						Genomic sequence
						
							TTGACATCATCTCTGCAATTGAGTTTGATAAATCTGGTGATCATCTTGCCACCGGAGACAGAGG
AGGACGTGTTGTTTTATTTGAAAGGACAGATGCTCGGGATGTAAGCACCATTAGTTGAACTTCT
CTTGCTCAATTTCATTATTTTATACTCATGAAAGTCTATCTGTTAGCAGAATGCCAGTCGGAGA
GAAATGGAGAGACAAGATGCTCCGATTACTAGACACCCAGAGTTTCGCTACAAAAGTGAATTTC
AGAGTCATGAACCTGAGGTATGTTGATAATCTGGATTGGCTGTTGTAGCACATAGTATTATCTG
TTATTTCCATGAAGTTGGACGCATAAGATCAAATTAACAAGCTGGCATTCTTATCTCAGAGCAA
GAAAGGAATCCTTAGCTTAACCATTGCAAGCAAATGAAGCATTACATTATTGTGCTATGCTCTT
GTTTTGTTCTTGCTTGAATTACTTGCATATGGAAACTCACATCTATGTTTTTTTGATGAACTGT
TCTAGTTTGATTACCTCAAAAGTTTGGAAATAGAGGAGAAGATCAACAAGATCAGGTGGTGCCA
GACAGCCAATAATTCACTCTCCCTTCTGTCTACAAATGATAAAACAATAAAATACTGGAAGGTA
TGGAGAAAAATATGTTAGGACCCAGGAATGGTTTTCAAGATTTCTGCATGCCAATATCTTTCTA
ATCTAATGTAACATTTAAGCCTGTCTTGACCAAGACCAACTCTGTTGGATGTTTGTATTTCTCC
GAACTGGTGGATCAAGATATTTATGTTGATTATTCCATCATTAGTGGAAAATTTAATGAAGTTT
ACATGAGCACTCTCTAGTTACGCAATGGAAATGATTTGTGATACCAGAGTACCAGACGTGATTT
TGTTACTACCTCTGTCCCATAATAATTGTATTTCTAGGATTTGAATTTGTCCCAAAATAATTGT
CACAATAGAGTACTAATGGCCCCATTAATCACTTCTCATTCAAATTTCTCCCTATTTTACCCTC
AACTACTCTTCCACTCTTACCTATACACCATTTAATAAGGGACATTATAGTCTTTCTTCTCAAA
GCTTAATATGCAGTAAACAACCTAGAATTACAATTATTTTGGGAAGGAAGTAGTATTAATTTTT
AACCTTTCGAAGCACCAACAAGATGCATTTGTTTAATCGGTGGTCGTACACGAGCTATGCCTTT
TCTTTTCTTTTTTCCTCACAAAATATCCACAATCCAAGTATTAGCTGTTTTGACCTTGGTTACA
TATTGCTTTACTTCCTTTACAGGTGCAAGAGAAGAAAGTAAAACAAGTTTCAGTTATGAATTTG
GATTCACGGAGTGTAGGGACTGGTACCTCTTCTAGTGCAAGTACTAGTAGTTCTAGGGGTCTTC
TTCCAAATGGTGGATGTTCAGACAAGTCCAGTTTCTTGAACAGTGACATTTTGTTTCCACCTGG
AGGCTACCCGTCATTACGTTTGCCTGTGGTAGTTGTACTTAACCTCTATATATCTTAATCAACA
GAAGATGACTTGATGTCTTGATCAGCTCTAGCACATCTAGCTGGCATATGATAACTTGCACGGC
AACCTATGATCAAGTCCAAAATTGACTGCATTTCTTATCATGCAGGTTGCAAGTCAAGATGTGA
ACCTTGTTGCTAGATGTCGACGTGTATATGCACATGCTCATGATTACCATATTAACTCTATATC
AACCAATAGGTAAGTATGATCATTGCATTGAATATTAATCTATTAAGCTGTATCCCTAATGCAT
TTTTTCTCCATGAGTTCATCTGTATCTATTTCTTTTCATGTTGAAATAAAATAATATCGAGCCG
AGCATCCAATTCTCATGGATTGCTCTTATTGTAAAACCAATAGTCAGTGCAATAGTCATGACAC
ATTGAATCAGCTTTAAAATCAGTGCAAACCATTGTTGTAGAGGCATACACTTTTTTATATTACA
ACAGTAACGCAATAGAGTTATCTGCAGTTTTTCTACCTGTATATTCCATATACTTTTTCAATGA
TCTAATTTGTGAACATTGTGCTGATTTGCTTCTCTTTTTCATTTAATTGTCAGTGATGGCGAGA
CATACATATCAGCAGATGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTT
CAATATTGTTGACGTGAAGCCTCCAAACATGGAAGACCTAACGG
						
					

			
		

Sequences
Splice junction sequence	TCAGCAGATGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTTCAATATTG TTGACGTGAAGCCTCCAAACATGGAAGACCTAACGGttgacatcatctctgcaattgagtttga taaatctggtgatcatcttgccaccggagacagaggaggacgtgttgttttatttgaaaggaca gatgctcg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 732 Transcript exons: 21656567-21656670,21656744-21656839,21657084-21657203,21 657869-21658069,21658212-21658303,21658668-21658786 TTGACATCATCTCTGCAATTGAGTTTGATAAATCTGGTGATCATCTTGCCACCGGAGACAGAGG AGGACGTGTTGTTTTATTTGAAAGGACAGATGCTCGGGATAATGCCAGTCGGAGAGAAATGGAG AGACAAGATGCTCCGATTACTAGACACCCAGAGTTTCGCTACAAAAGTGAATTTCAGAGTCATG AACCTGAGTTTGATTACCTCAAAAGTTTGGAAATAGAGGAGAAGATCAACAAGATCAGGTGGTG CCAGACAGCCAATAATTCACTCTCCCTTCTGTCTACAAATGATAAAACAATAAAATACTGGAAG GTGCAAGAGAAGAAAGTAAAACAAGTTTCAGTTATGAATTTGGATTCACGGAGTGTAGGGACTG GTACCTCTTCTAGTGCAAGTACTAGTAGTTCTAGGGGTCTTCTTCCAAATGGTGGATGTTCAGA CAAGTCCAGTTTCTTGAACAGTGACATTTTGTTTCCACCTGGAGGCTACCCGTCATTACGTTTG CCTGTGGTAGTTGCAAGTCAAGATGTGAACCTTGTTGCTAGATGTCGACGTGTATATGCACATG CTCATGATTACCATATTAACTCTATATCAACCAATAGTGATGGCGAGACATACATATCAGCAGA TGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTTCAATATTGTTGACGTG AAGCCTCCAAACATGGAAGACCTAACGG
Genomic sequence	TTGACATCATCTCTGCAATTGAGTTTGATAAATCTGGTGATCATCTTGCCACCGGAGACAGAGG AGGACGTGTTGTTTTATTTGAAAGGACAGATGCTCGGGATGTAAGCACCATTAGTTGAACTTCT CTTGCTCAATTTCATTATTTTATACTCATGAAAGTCTATCTGTTAGCAGAATGCCAGTCGGAGA GAAATGGAGAGACAAGATGCTCCGATTACTAGACACCCAGAGTTTCGCTACAAAAGTGAATTTC AGAGTCATGAACCTGAGGTATGTTGATAATCTGGATTGGCTGTTGTAGCACATAGTATTATCTG TTATTTCCATGAAGTTGGACGCATAAGATCAAATTAACAAGCTGGCATTCTTATCTCAGAGCAA GAAAGGAATCCTTAGCTTAACCATTGCAAGCAAATGAAGCATTACATTATTGTGCTATGCTCTT GTTTTGTTCTTGCTTGAATTACTTGCATATGGAAACTCACATCTATGTTTTTTTGATGAACTGT TCTAGTTTGATTACCTCAAAAGTTTGGAAATAGAGGAGAAGATCAACAAGATCAGGTGGTGCCA GACAGCCAATAATTCACTCTCCCTTCTGTCTACAAATGATAAAACAATAAAATACTGGAAGGTA TGGAGAAAAATATGTTAGGACCCAGGAATGGTTTTCAAGATTTCTGCATGCCAATATCTTTCTA ATCTAATGTAACATTTAAGCCTGTCTTGACCAAGACCAACTCTGTTGGATGTTTGTATTTCTCC GAACTGGTGGATCAAGATATTTATGTTGATTATTCCATCATTAGTGGAAAATTTAATGAAGTTT ACATGAGCACTCTCTAGTTACGCAATGGAAATGATTTGTGATACCAGAGTACCAGACGTGATTT TGTTACTACCTCTGTCCCATAATAATTGTATTTCTAGGATTTGAATTTGTCCCAAAATAATTGT CACAATAGAGTACTAATGGCCCCATTAATCACTTCTCATTCAAATTTCTCCCTATTTTACCCTC AACTACTCTTCCACTCTTACCTATACACCATTTAATAAGGGACATTATAGTCTTTCTTCTCAAA GCTTAATATGCAGTAAACAACCTAGAATTACAATTATTTTGGGAAGGAAGTAGTATTAATTTTT AACCTTTCGAAGCACCAACAAGATGCATTTGTTTAATCGGTGGTCGTACACGAGCTATGCCTTT TCTTTTCTTTTTTCCTCACAAAATATCCACAATCCAAGTATTAGCTGTTTTGACCTTGGTTACA TATTGCTTTACTTCCTTTACAGGTGCAAGAGAAGAAAGTAAAACAAGTTTCAGTTATGAATTTG GATTCACGGAGTGTAGGGACTGGTACCTCTTCTAGTGCAAGTACTAGTAGTTCTAGGGGTCTTC TTCCAAATGGTGGATGTTCAGACAAGTCCAGTTTCTTGAACAGTGACATTTTGTTTCCACCTGG AGGCTACCCGTCATTACGTTTGCCTGTGGTAGTTGTACTTAACCTCTATATATCTTAATCAACA GAAGATGACTTGATGTCTTGATCAGCTCTAGCACATCTAGCTGGCATATGATAACTTGCACGGC AACCTATGATCAAGTCCAAAATTGACTGCATTTCTTATCATGCAGGTTGCAAGTCAAGATGTGA ACCTTGTTGCTAGATGTCGACGTGTATATGCACATGCTCATGATTACCATATTAACTCTATATC AACCAATAGGTAAGTATGATCATTGCATTGAATATTAATCTATTAAGCTGTATCCCTAATGCAT TTTTTCTCCATGAGTTCATCTGTATCTATTTCTTTTCATGTTGAAATAAAATAATATCGAGCCG AGCATCCAATTCTCATGGATTGCTCTTATTGTAAAACCAATAGTCAGTGCAATAGTCATGACAC ATTGAATCAGCTTTAAAATCAGTGCAAACCATTGTTGTAGAGGCATACACTTTTTTATATTACA ACAGTAACGCAATAGAGTTATCTGCAGTTTTTCTACCTGTATATTCCATATACTTTTTCAATGA TCTAATTTGTGAACATTGTGCTGATTTGCTTCTCTTTTTCATTTAATTGTCAGTGATGGCGAGA CATACATATCAGCAGATGATCTGCGTATAAATCTATGGAATTTGGAGATAAATAACCAGAGCTT CAATATTGTTGACGTGAAGCCTCCAAACATGGAAGACCTAACGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016330 osi_circ_003765 						
					
						PMCS
						0.342768866

Conservation Information
Conserved circRNAs	osi_circ_016330 osi_circ_003765
PMCS	0.342768866

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							21656763-21658783(+)
21656578-21658276(-)
						
						Potential amino acid sequence
						
							MERQDAPITRHPEFRYKSEFQSHEPEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKY
WKVQEKKVKQVSVMNLDSRSVGTGTSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSL
RLPVVVASQDVNLVARCRRVYAHAHDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIV
DVKPPNMEDLTVDIISAIEFDKSGDHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHP
EFRYKSEFQSHEPEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVS
VMNLDSRSVGTGTSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVN
LVARCRRVYAHAHDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLTV
DIISAIEFDKSGDHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHPEFRYKSEFQSHE
PEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVSVMNLDSRSVGTG
TSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVNLVARCRRVYAHA
HDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLTVDIISAIEFDKSG
DHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHPEFRYKSEFQSHEPEFDYLKSLEIE
EKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVSVMNLDSRSVGTGTSSSASTSSSRG
LLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVNLVARCRRVYAHAHDYHINSISTNS
DGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLT(+)
MMSTVRSSMFGGFTSTILKLWLFISKFHRFIRRSSADMYVSPSLLVDIELIW*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	21656763-21658783(+) 21656578-21658276(-)
Potential amino acid sequence	MERQDAPITRHPEFRYKSEFQSHEPEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKY WKVQEKKVKQVSVMNLDSRSVGTGTSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSL RLPVVVASQDVNLVARCRRVYAHAHDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIV DVKPPNMEDLTVDIISAIEFDKSGDHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHP EFRYKSEFQSHEPEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVS VMNLDSRSVGTGTSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVN LVARCRRVYAHAHDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLTV DIISAIEFDKSGDHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHPEFRYKSEFQSHE PEFDYLKSLEIEEKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVSVMNLDSRSVGTG TSSSASTSSSRGLLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVNLVARCRRVYAHA HDYHINSISTNSDGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLTVDIISAIEFDKSG DHLATGDRGGRVVLFERTDARDNASRREMERQDAPITRHPEFRYKSEFQSHEPEFDYLKSLEIE EKINKIRWCQTANNSLSLLSTNDKTIKYWKVQEKKVKQVSVMNLDSRSVGTGTSSSASTSSSRG LLPNGGCSDKSSFLNSDILFPPGGYPSLRLPVVVASQDVNLVARCRRVYAHAHDYHINSISTNS DGETYISADDLRINLWNLEINNQSFNIVDVKPPNMEDLT(+) MMSTVRSSMFGGFTSTILKLWLFISKFHRFIRRSSADMYVSPSLLVDIELIW*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os06g0563250_circ_g.2

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