Plant Circular RNA Database

Detail information of Zm00001d034018_circ_g.5

			
						 General Information
					
						CircRNA Name
						Zm00001d034018_circ_g.5
					
						ID in PlantcircBase
						zma_circ_006931
					
						Alias
						Zm01circ00179, GRMZM2G016671_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr1: 281266932-281267167  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d034018						
					
						Parent gene annotation
						
							Tousled-like kinase 1  
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d034018_circ_g.1 Zm00001d034018_circ_g.2 Zm00001d034018_circ_g.3 Zm00001d034018_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d034018_T001:2
Zm00001d034018_T041:2
Zm00001d034018_T008:2
Zm00001d034018_T044:2
Zm00001d034018_T038:2
Zm00001d034018_T045:2
Zm00001d034018_T002:2
Zm00001d034018_T029:2
Zm00001d034018_T035:2
Zm00001d034018_T023:2
Zm00001d034018_T039:2
Zm00001d034018_T036:2
Zm00001d034018_T046:2
Zm00001d034018_T007:2
Zm00001d034018_T016:2
Zm00001d034018_T003:2
Zm00001d034018_T021:2
Zm00001d034018_T010:2
Zm00001d034018_T006:2
Zm00001d034018_T043:2
Zm00001d034018_T026:2
Zm00001d034018_T019:2
Zm00001d034018_T024:2
Zm00001d034018_T034:2
Zm00001d034018_T012:2
Zm00001d034018_T020:2
Zm00001d034018_T030:2
Zm00001d034018_T042:2
Zm00001d034018_T028:2
Zm00001d034018_T025:2
Zm00001d034018_T031:2
Zm00001d034018_T005:2
Zm00001d034018_T040:2
Zm00001d034018_T004:2
Zm00001d034018_T009:2
Zm00001d034018_T017:2
Zm00001d034018_T015:2
Zm00001d034018_T037:2
Zm00001d034018_T013:2
Zm00001d034018_T014:2
Zm00001d034018_T022:2
Zm00001d034018_T027:2
Zm00001d034018_T033:2

General Information
CircRNA Name	Zm00001d034018_circ_g.5
ID in PlantcircBase	zma_circ_006931
Alias	Zm01circ00179, GRMZM2G016671_C1
Organism	Zea mays
Position	chr1: 281266932-281267167 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2
Parent gene	Zm00001d034018
Parent gene annotation	Tousled-like kinase 1
Parent gene strand	-
Alternative splicing	Zm00001d034018_circ_g.1 Zm00001d034018_circ_g.2 Zm00001d034018_circ_g.3 Zm00001d034018_circ_g.4
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d034018_T001:2 Zm00001d034018_T041:2 Zm00001d034018_T008:2 Zm00001d034018_T044:2 Zm00001d034018_T038:2 Zm00001d034018_T045:2 Zm00001d034018_T002:2 Zm00001d034018_T029:2 Zm00001d034018_T035:2 Zm00001d034018_T023:2 Zm00001d034018_T039:2 Zm00001d034018_T036:2 Zm00001d034018_T046:2 Zm00001d034018_T007:2 Zm00001d034018_T016:2 Zm00001d034018_T003:2 Zm00001d034018_T021:2 Zm00001d034018_T010:2 Zm00001d034018_T006:2 Zm00001d034018_T043:2 Zm00001d034018_T026:2 Zm00001d034018_T019:2 Zm00001d034018_T024:2 Zm00001d034018_T034:2 Zm00001d034018_T012:2 Zm00001d034018_T020:2 Zm00001d034018_T030:2 Zm00001d034018_T042:2 Zm00001d034018_T028:2 Zm00001d034018_T025:2 Zm00001d034018_T031:2 Zm00001d034018_T005:2 Zm00001d034018_T040:2 Zm00001d034018_T004:2 Zm00001d034018_T009:2 Zm00001d034018_T017:2 Zm00001d034018_T015:2 Zm00001d034018_T037:2 Zm00001d034018_T013:2 Zm00001d034018_T014:2 Zm00001d034018_T022:2 Zm00001d034018_T027:2 Zm00001d034018_T033:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACCTATCACTAAGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCTTCCTCCAA
AAATGCAACTTTGCCACGTAATGTTGACAATTCGTCctttaatctcatttgttggtcatgatct
ttgagatccttcaattcctggaagaataatgcaaagctaacatgtcatgacactaaaaaataca
tgtttaaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 153
Transcript exons: 281266932-281266976,281267060-281267167
CTTTAATCTCATTTGTTGGTCATGATCTTTGAGATCCTTCAATTCCTTTGCTAACCTATCACTA
AGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCTTCCTCCAAAAATGCAACTT
TGCCACGTAATGTTGACAATTCGTC
						
						Genomic sequence
						
							CTTTAATCTCATTTGTTGGTCATGATCTTTGAGATCCTTCAATTCCTGGAAGAATAATGCAAAG
CTAACATGTCATGACACTAAAAAATACATGTTTAAATGAAAACTGAAAGTAAGTATATCCTTAC
CTTTGCTAACCTATCACTAAGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCT
TCCTCCAAAAATGCAACTTTGCCACGTAATGTTGACAATTCGTC

Sequences
Splice junction sequence	ACCTATCACTAAGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCTTCCTCCAA AAATGCAACTTTGCCACGTAATGTTGACAATTCGTCctttaatctcatttgttggtcatgatct ttgagatccttcaattcctggaagaataatgcaaagctaacatgtcatgacactaaaaaataca tgtttaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 153 Transcript exons: 281266932-281266976,281267060-281267167 CTTTAATCTCATTTGTTGGTCATGATCTTTGAGATCCTTCAATTCCTTTGCTAACCTATCACTA AGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCTTCCTCCAAAAATGCAACTT TGCCACGTAATGTTGACAATTCGTC
Genomic sequence	CTTTAATCTCATTTGTTGGTCATGATCTTTGAGATCCTTCAATTCCTGGAAGAATAATGCAAAG CTAACATGTCATGACACTAAAAAATACATGTTTAAATGAAAACTGAAAGTAAGTATATCCTTAC CTTTGCTAACCTATCACTAAGTTCATGGTAATTTGTTGCTTCTTGATGCGATTTAGAAAGGTCT TCCTCCAAAAATGCAACTTTGCCACGTAATGTTGACAATTCGTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.229896628

Conservation Information
Conserved circRNAs	NA
PMCS	0.229896628

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							281267135-281266960(+)
281266945-281266934(-)
						
						Potential amino acid sequence
						
							MQLCHVMLTIRPLISFVGHDL*(+)
MRLKDELSTLRGKVAFLEEDLSKSHQEATNYHELSDRLAKELKDLKDHDQQMRLKDELSTLRGK
VAFLEEDLSKSHQEATNYHELSDRLAKELKDLKDHDQQMRLKDELSTLRGKVAFLEEDLSKSHQ
EATNYHELSDRLAKELKDLKDHDQQMRLK(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	281267135-281266960(+) 281266945-281266934(-)
Potential amino acid sequence	MQLCHVMLTIRPLISFVGHDL*(+) MRLKDELSTLRGKVAFLEEDLSKSHQEATNYHELSDRLAKELKDLKDHDQQMRLKDELSTLRGK VAFLEEDLSKSHQEATNYHELSDRLAKELKDLKDHDQQMRLKDELSTLRGKVAFLEEDLSKSHQ EATNYHELSDRLAKELKDLKDHDQQMRLK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d034018_circ_g.5

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