Plant Circular RNA Database

Detail information of Os03g0825300_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0825300_circ_g.4
					
						ID in PlantcircBase
						osa_circ_022520
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 34665232-34667448  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0825300						
					
						Parent gene annotation
						
							Serine/threonine protein kinase domain containing protein. (Os03
t0825300-01);Serine/threonine protein kinase domain containing p
rotein. (Os03t0825300-02);Serine/threonine protein kinase domain
 containing protein. (Os03t0825300-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0825300_circ_g.1 Os03g0825300_circ_g.2 Os03g0825300_circ_g.3 Os03g0825300_circ_g.5 Os03g0825300_circ_g.6 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0825300-01:6
Os03t0825300-03:6
Os03t0825300-02:6

General Information
CircRNA Name	Os03g0825300_circ_g.4
ID in PlantcircBase	osa_circ_022520
Alias	NA
Organism	Oryza sativa
Position	chr3: 34665232-34667448 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0825300
Parent gene annotation	Serine/threonine protein kinase domain containing protein. (Os03 t0825300-01);Serine/threonine protein kinase domain containing p rotein. (Os03t0825300-02);Serine/threonine protein kinase domain containing protein. (Os03t0825300-03)
Parent gene strand	+
Alternative splicing	Os03g0825300_circ_g.1 Os03g0825300_circ_g.2 Os03g0825300_circ_g.3 Os03g0825300_circ_g.5 Os03g0825300_circ_g.6
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0825300-01:6 Os03t0825300-03:6 Os03t0825300-02:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GACTGGCCAACAAGTATCTGATAGTATTCTTGTCAAGAAACATGGAGACAGTGCTTTTCAATCC
AAGGATTTTGCGACTGCAGTTGAGTGCTACTCAAGGgggagacaaagccattgagctgggcaat
gagggtacgagctgcattctatgtggctcaggcattggagtactgcagtagcaaagggagagct
ctctacca

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GGGAGACAAAGCCATTGAGCTGGGCAATGAGGGTACGAGCTGCATTCTATGTGGCTCAGGCATT
GGAGTACTGCAGTAGCAAAGGGAGAGCTCTCTACCATGATCTGCATGCATACAGGGTTCTCTTT
GATGTGGTGAGTCAGTACCTGTGCTACTTAGTAACTTCTTAGCTTGGGCTTCCAGTAATAAGGC
ATGTAATGTTTTGATGATAAATCATAGGATGGTAACCCCAGACTGTCGTGCTTCGGTCTGATGA
AGAACAGCAGGGATGGGAAGAGCTATAGTACCAATTTGGCTTTCACGCCTCCTGAGTACCTTAA
GACTGGTATGTGTTGTGCATACAAGCAAGTTTTTTTCTGTGAGTTATGCATTTCATGAATTTTA
TTTGTTAATGTTTGATCATGAAGTAAGGACTTGTAATATGAGCGTAATATGAGCTAACTCTTAG
ACACTTGTTGAGTATGTCCATGTTCTTTGCTAGTTATTTTCCCCAGTGGGGTAAATCATTCTAT
GCAAATGTTATTGCTGGGTTTCCATTGTTTATTTTTAGCTATTCACACCTTGTTTATTTTTAGC
TATGGTACCATGGTTATGAACCACAGACATTAGGCAACAACGTAAGCTGCACGGACTTGTTTTT
CATGAAGGGATAGTATTTAGCATTCGACCTCTCTCATAGTGAAGCAACAGAATTCTGTGTTGCA
AAATCTACCTCGTTTTCTTATGACTTTGTCCATCAAAGACACAGTCTCTATTTCTTTACTAACC
ACTACATTTTCTGCAGGCAGAGTAATTCCAGAGAGTGTGGTGTACAGTTTTGGTACTATCTTGC
TTGATCTCCTAAGTGGAAAACATATCCCACCAAGCCATGTAAGTTATCTATCCTTCCTGCTTTT
CTGCTTACCCAAGATAAGTATTCACTTTGCTTGGTTTTGCTGTAGGCGCTTGACCTTATAAGAG
GAAAAAATTACCTGGTGTTGATGGACTCTTGCTTAGAAGGCCATGTCTCCAGCTCTGATGGAAC
TGAGTTGATACGATTAGCATCCCGTTGCTTGCAGTATGAAGGTCGTGACCGGCCAAACCTGAAA
TCGGTGGTGTCTGCTCTTGGAAACCTCCAGAAAGATGCTTCTGTAATGTTTCTTTTTCTTGCTA
CTTTCTAGACTAGTAACAGTATTGTGTAATATTGAAAAAGCTGCAGGAAAAAAACTCCACACAA
TCGTTTGATAACTATTTTGTCTTCTGTCAACGTGCTACCTTTGCTTTTCATTTGATATTATGTT
AGCATAAATGGTTTTTTGAAGTATGCGCCAACTCATCTTTCTCCTTATTAATGTGTCGCGCCTG
CCAGCTTAATATTTCTTACTCGATGTTTAATTGTCTGTGCAGGCTCCTTCACATGCTTTACTAG
GCATACAACATGATAAGGAAAATACAGAGCGAATTTCGTTATCTGCTATAGCGAAAGTTTATGC
CAGAGCAGACCTTGATGAGGTTCATGAGATGCTGGAGAATGATGGATATTGTGAGGATGAAAGA
GCAACATTCGAGGTATTAACTTTTTGTCAGTTAAGCTTATCATAATGAAATATTCTTGTTGGTA
CCATAGATCCTTCCCTAACTCATTGCTGCCATTTCCCTAAAAAGGAATAAACTGCCGGAACTTA
TTACTACATTATTGTCATCTTGGAATATTTCAATTGTGTAGATGAAACAAAATATGTTGAAAGC
CCACACATTTTGTGGCATAGGATAGGAAGAAAGTAGATGGTCTATGTTGATGCCCATGCTCTTC
GACCAATGAAACCTAATGGTTAGCTCATGTTTTAAGTGCCATTCTTTTGAGCTCGAGGCTAAAC
TTTTAACTAGTTTGACCATGCTTATTTTTATTGCTCTATAGGTCTGAAAGTACAATAGAACATT
TATGTATCTTTTGAAGATATATTATACATCCATCAGTTGACCTGTAATATATCCCATACAAAAC
TTAACTTCTCACCATGTTGGCTGGTCCAAGTTTTTTTAACGTGAAGGTTTTCCATGTTTTCGCA
CTGCTCCCGTTCATATCTGACCCATACCTTAAAAATTTGGTGTATATAACAGGTATCTTTCCAT
TCCTGGACTGGCCAACAAGTATCTGATAGTATTCTTGTCAAGAAACATGGAGACAGTGCTTTTC
AATCCAAGGATTTTGCGACTGCAGTTGAGTGCTACTCAAGG
						
					

			
		

Sequences
Splice junction sequence	GACTGGCCAACAAGTATCTGATAGTATTCTTGTCAAGAAACATGGAGACAGTGCTTTTCAATCC AAGGATTTTGCGACTGCAGTTGAGTGCTACTCAAGGgggagacaaagccattgagctgggcaat gagggtacgagctgcattctatgtggctcaggcattggagtactgcagtagcaaagggagagct ctctacca
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGGAGACAAAGCCATTGAGCTGGGCAATGAGGGTACGAGCTGCATTCTATGTGGCTCAGGCATT GGAGTACTGCAGTAGCAAAGGGAGAGCTCTCTACCATGATCTGCATGCATACAGGGTTCTCTTT GATGTGGTGAGTCAGTACCTGTGCTACTTAGTAACTTCTTAGCTTGGGCTTCCAGTAATAAGGC ATGTAATGTTTTGATGATAAATCATAGGATGGTAACCCCAGACTGTCGTGCTTCGGTCTGATGA AGAACAGCAGGGATGGGAAGAGCTATAGTACCAATTTGGCTTTCACGCCTCCTGAGTACCTTAA GACTGGTATGTGTTGTGCATACAAGCAAGTTTTTTTCTGTGAGTTATGCATTTCATGAATTTTA TTTGTTAATGTTTGATCATGAAGTAAGGACTTGTAATATGAGCGTAATATGAGCTAACTCTTAG ACACTTGTTGAGTATGTCCATGTTCTTTGCTAGTTATTTTCCCCAGTGGGGTAAATCATTCTAT GCAAATGTTATTGCTGGGTTTCCATTGTTTATTTTTAGCTATTCACACCTTGTTTATTTTTAGC TATGGTACCATGGTTATGAACCACAGACATTAGGCAACAACGTAAGCTGCACGGACTTGTTTTT CATGAAGGGATAGTATTTAGCATTCGACCTCTCTCATAGTGAAGCAACAGAATTCTGTGTTGCA AAATCTACCTCGTTTTCTTATGACTTTGTCCATCAAAGACACAGTCTCTATTTCTTTACTAACC ACTACATTTTCTGCAGGCAGAGTAATTCCAGAGAGTGTGGTGTACAGTTTTGGTACTATCTTGC TTGATCTCCTAAGTGGAAAACATATCCCACCAAGCCATGTAAGTTATCTATCCTTCCTGCTTTT CTGCTTACCCAAGATAAGTATTCACTTTGCTTGGTTTTGCTGTAGGCGCTTGACCTTATAAGAG GAAAAAATTACCTGGTGTTGATGGACTCTTGCTTAGAAGGCCATGTCTCCAGCTCTGATGGAAC TGAGTTGATACGATTAGCATCCCGTTGCTTGCAGTATGAAGGTCGTGACCGGCCAAACCTGAAA TCGGTGGTGTCTGCTCTTGGAAACCTCCAGAAAGATGCTTCTGTAATGTTTCTTTTTCTTGCTA CTTTCTAGACTAGTAACAGTATTGTGTAATATTGAAAAAGCTGCAGGAAAAAAACTCCACACAA TCGTTTGATAACTATTTTGTCTTCTGTCAACGTGCTACCTTTGCTTTTCATTTGATATTATGTT AGCATAAATGGTTTTTTGAAGTATGCGCCAACTCATCTTTCTCCTTATTAATGTGTCGCGCCTG CCAGCTTAATATTTCTTACTCGATGTTTAATTGTCTGTGCAGGCTCCTTCACATGCTTTACTAG GCATACAACATGATAAGGAAAATACAGAGCGAATTTCGTTATCTGCTATAGCGAAAGTTTATGC CAGAGCAGACCTTGATGAGGTTCATGAGATGCTGGAGAATGATGGATATTGTGAGGATGAAAGA GCAACATTCGAGGTATTAACTTTTTGTCAGTTAAGCTTATCATAATGAAATATTCTTGTTGGTA CCATAGATCCTTCCCTAACTCATTGCTGCCATTTCCCTAAAAAGGAATAAACTGCCGGAACTTA TTACTACATTATTGTCATCTTGGAATATTTCAATTGTGTAGATGAAACAAAATATGTTGAAAGC CCACACATTTTGTGGCATAGGATAGGAAGAAAGTAGATGGTCTATGTTGATGCCCATGCTCTTC GACCAATGAAACCTAATGGTTAGCTCATGTTTTAAGTGCCATTCTTTTGAGCTCGAGGCTAAAC TTTTAACTAGTTTGACCATGCTTATTTTTATTGCTCTATAGGTCTGAAAGTACAATAGAACATT TATGTATCTTTTGAAGATATATTATACATCCATCAGTTGACCTGTAATATATCCCATACAAAAC TTAACTTCTCACCATGTTGGCTGGTCCAAGTTTTTTTAACGTGAAGGTTTTCCATGTTTTCGCA CTGCTCCCGTTCATATCTGACCCATACCTTAAAAATTTGGTGTATATAACAGGTATCTTTCCAT TCCTGGACTGGCCAACAAGTATCTGATAGTATTCTTGTCAAGAAACATGGAGACAGTGCTTTTC AATCCAAGGATTTTGCGACTGCAGTTGAGTGCTACTCAAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.12537167

Conservation Information
Conserved circRNAs	NA
PMCS	0.12537167

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							34666235-34665307(+)
34665259-34665246(+)
34665343-34667441(-)
34665347-34667445(-)
						
						Potential amino acid sequence
						
							MSPALMRFMRCWRMMDIVRMKEQHSRYLSIPGLANKYLIVFLSRNMETVLFNPRILRLQLSATQ
GGDKAIELGNEGTSCILCGSGIGVLQ*(+)
MRVRAAFYVAQALEYCSSKGRALYHDLHAYRVLFDVDGNPRLSCFGLMKNSRDGKSYSTNLAFT
PPEYLKTGRVIPESVVYSFGTILLDLLSGKHIPPSHALDLIRGKNYLVLMDSCLEGHVSSSDEV
HEMLENDGYCEDERATFEVSFHSWTGQQVSDSILVKKHGDSAFQSKDFATAVECYSRGRQSH*(
+)
MQIMVESSPFATAVLQCLSHIECSSYPHCPAQWLCLPLE*(-)
MHADHGRELSLCYCSTPMPEPHRMQLVPSLPSSMALSPP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	34666235-34665307(+) 34665259-34665246(+) 34665343-34667441(-) 34665347-34667445(-)
Potential amino acid sequence	MSPALMRFMRCWRMMDIVRMKEQHSRYLSIPGLANKYLIVFLSRNMETVLFNPRILRLQLSATQ GGDKAIELGNEGTSCILCGSGIGVLQ(+) MRVRAAFYVAQALEYCSSKGRALYHDLHAYRVLFDVDGNPRLSCFGLMKNSRDGKSYSTNLAFT PPEYLKTGRVIPESVVYSFGTILLDLLSGKHIPPSHALDLIRGKNYLVLMDSCLEGHVSSSDEV HEMLENDGYCEDERATFEVSFHSWTGQQVSDSILVKKHGDSAFQSKDFATAVECYSRGRQSH( +) MQIMVESSPFATAVLQCLSHIECSSYPHCPAQWLCLPLE(-) MHADHGRELSLCYCSTPMPEPHRMQLVPSLPSSMALSPP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0825300_circ_g.4

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