Plant Circular RNA Database

Detail information of Zm00001d041108_circ_g.5

			
						 General Information
					
						CircRNA Name
						Zm00001d041108_circ_g.5
					
						ID in PlantcircBase
						zma_circ_007609
					
						Alias
						Zm03circ00034, zma_circ_0001097, GRMZM2G135322_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr3: 97778909-97779717  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d041108						
					
						Parent gene annotation
						
							Probable Ufm1-specific protease
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d041108_circ_g.1 Zm00001d041108_circ_g.2 Zm00001d041108_circ_g.3 Zm00001d041108_circ_g.4 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d041108_T008:4
Zm00001d041108_T012:3
Zm00001d041108_T002:4
Zm00001d041108_T011:4
Zm00001d041108_T004:4
Zm00001d041108_T014:4
Zm00001d041108_T005:4
Zm00001d041108_T007:2
Zm00001d041108_T020:4
Zm00001d041108_T013:2
Zm00001d041108_T016:4
Zm00001d041108_T021:4
Zm00001d041108_T009:4
Zm00001d041108_T018:4
Zm00001d041108_T010:4
Zm00001d041108_T001:2
Zm00001d041108_T015:4
Zm00001d041108_T003:4
Zm00001d041108_T019:4
Zm00001d041108_T017:4

General Information
CircRNA Name	Zm00001d041108_circ_g.5
ID in PlantcircBase	zma_circ_007609
Alias	Zm03circ00034, zma_circ_0001097, GRMZM2G135322_C1
Organism	Zea mays
Position	chr3: 97778909-97779717 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d041108
Parent gene annotation	Probable Ufm1-specific protease
Parent gene strand	+
Alternative splicing	Zm00001d041108_circ_g.1 Zm00001d041108_circ_g.2 Zm00001d041108_circ_g.3 Zm00001d041108_circ_g.4
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d041108_T008:4 Zm00001d041108_T012:3 Zm00001d041108_T002:4 Zm00001d041108_T011:4 Zm00001d041108_T004:4 Zm00001d041108_T014:4 Zm00001d041108_T005:4 Zm00001d041108_T007:2 Zm00001d041108_T020:4 Zm00001d041108_T013:2 Zm00001d041108_T016:4 Zm00001d041108_T021:4 Zm00001d041108_T009:4 Zm00001d041108_T018:4 Zm00001d041108_T010:4 Zm00001d041108_T001:2 Zm00001d041108_T015:4 Zm00001d041108_T003:4 Zm00001d041108_T019:4 Zm00001d041108_T017:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTAAAGTTTTATGTTTATTGCAGTATCCGGAGGTGTTATGTCTTTGATTGATGGTTCATATGAG
TATTATCATTATCTTCACGATGGTATTGATGACAATcttcaaccattccattttattccttctg
gttggcatgttccagtaacagctatatacaatacaagatatggtgagactgaggagaggcaaag
tatgaaaa

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CTTCAACCATTCCATTTTATTCCTTCTGGTTGGCATGTTCCAGTAACAGCTATATACAATACAA
GATATGGTGAGACTGAGGAGAGGCAAAGTATGAAAAATTCTATTTCTGAAAAGTTCATAAATTC
TTTCTTGAAGCACGAGAGTGAATATAGGACCTTAATACTGCCTCTGTCTCTATCTAAAAATATC
CTTTATTATTTACAGGTGAGCTGCGAAAAGAACTGCATTTAAGGCTAGGACTTCCCTTAGATCG
GCCTTTGTTGCGAATTTCTAATGCATTGACTTTCGGTGGCATGGGAAGGAGGAAGAAAAGCATG
CCAAGAAGTGGTATTCACCATGCATATGTTGCGACATATGCTTCATTACTCTGTTTTCCCCCTA
AATTACATGTGGGATCTTGTGGTAACTTTGTGCAAAAAATGTTTCAAAGTATAATATATATAAG
AACTGCAAAATATCATATTATACTATGTGAGAAGTTAATAACTTCATCTAACATGAAGATAAGA
ACTTATCTTTGTATATGTAATTCCTCAGGAAGTAGAATTTGTTTTCATCCATTGTGGTATGTTA
TCACATTCAGGTTCTTCGCTGCTTCGAGACATCCACAGAGAACTTCCATCTAGTGGTGGTTAGT
TGTATCTCTATTTCTTGCTTTAAGCAAAATAAGAAATTTGGTGAATGTATTCTAGAAGGAGATA
GTGACGTAAAGTTTTATGTTTATTGCAGTATCCGGAGGTGTTATGTCTTTGATTGATGGTTCAT
ATGAGTATTATCATTATCTTCACGATGGTATTGATGACAAT

Sequences
Splice junction sequence	GTAAAGTTTTATGTTTATTGCAGTATCCGGAGGTGTTATGTCTTTGATTGATGGTTCATATGAG TATTATCATTATCTTCACGATGGTATTGATGACAATcttcaaccattccattttattccttctg gttggcatgttccagtaacagctatatacaatacaagatatggtgagactgaggagaggcaaag tatgaaaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTTCAACCATTCCATTTTATTCCTTCTGGTTGGCATGTTCCAGTAACAGCTATATACAATACAA GATATGGTGAGACTGAGGAGAGGCAAAGTATGAAAAATTCTATTTCTGAAAAGTTCATAAATTC TTTCTTGAAGCACGAGAGTGAATATAGGACCTTAATACTGCCTCTGTCTCTATCTAAAAATATC CTTTATTATTTACAGGTGAGCTGCGAAAAGAACTGCATTTAAGGCTAGGACTTCCCTTAGATCG GCCTTTGTTGCGAATTTCTAATGCATTGACTTTCGGTGGCATGGGAAGGAGGAAGAAAAGCATG CCAAGAAGTGGTATTCACCATGCATATGTTGCGACATATGCTTCATTACTCTGTTTTCCCCCTA AATTACATGTGGGATCTTGTGGTAACTTTGTGCAAAAAATGTTTCAAAGTATAATATATATAAG AACTGCAAAATATCATATTATACTATGTGAGAAGTTAATAACTTCATCTAACATGAAGATAAGA ACTTATCTTTGTATATGTAATTCCTCAGGAAGTAGAATTTGTTTTCATCCATTGTGGTATGTTA TCACATTCAGGTTCTTCGCTGCTTCGAGACATCCACAGAGAACTTCCATCTAGTGGTGGTTAGT TGTATCTCTATTTCTTGCTTTAAGCAAAATAAGAAATTTGGTGAATGTATTCTAGAAGGAGATA GTGACGTAAAGTTTTATGTTTATTGCAGTATCCGGAGGTGTTATGTCTTTGATTGATGGTTCAT ATGAGTATTATCATTATCTTCACGATGGTATTGATGACAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.140910795

Conservation Information
Conserved circRNAs	NA
PMCS	0.140910795

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							97779206-97779714(+)
97779669-97778951(+)
97779140-97779675(-)
97778920-97779655(-)
						
						Potential amino acid sequence
						
							MGRRKKSMPRSGSSLLRDIHRELPSSGVSGGVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGW
HVPVTAIYNTRYGETEERQSELRKELHLRLGLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSL
LRDIHRELPSSGVSGGVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGWHVPVTAIYNTRYGET
EERQSELRKELHLRLGLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSLLRDIHRELPSSGVSG
GVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGWHVPVTAIYNTRYGETEERQSELRKELHLRL
GLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSLLRDIHRELPSSGVSGGVMSLIDGSYEYYHY
LHDGIDDN(+)
MVHMSIIIIFTMVLMTIFNHSILFLLVGMFQ*(+)
MQFFSQLTLPLLSLTISCIVYSCYWNMPTRRNKMEWLKIVINTIVKIMIILI*(-)
MVEDCHQYHREDNDNTHMNHQSKT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress; regulating the response to N deficiency

Functional Information
Coding potential	Y
Potential coding position	97779206-97779714(+) 97779669-97778951(+) 97779140-97779675(-) 97778920-97779655(-)
Potential amino acid sequence	MGRRKKSMPRSGSSLLRDIHRELPSSGVSGGVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGW HVPVTAIYNTRYGETEERQSELRKELHLRLGLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSL LRDIHRELPSSGVSGGVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGWHVPVTAIYNTRYGET EERQSELRKELHLRLGLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSLLRDIHRELPSSGVSG GVMSLIDGSYEYYHYLHDGIDDNLQPFHFIPSGWHVPVTAIYNTRYGETEERQSELRKELHLRL GLPLDRPLLRISNALTFGGMGRRKKSMPRSGSSLLRDIHRELPSSGVSGGVMSLIDGSYEYYHY LHDGIDDN(+) MVHMSIIIIFTMVLMTIFNHSILFLLVGMFQ(+) MQFFSQLTLPLLSLTISCIVYSCYWNMPTRRNKMEWLKIVINTIVKIMIILI(-) MVEDCHQYHREDNDNTHMNHQSKT*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress; regulating the response to N deficiency

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d041108_circ_g.5

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