Plant Circular RNA Database

Detail information of Os06g0644500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os06g0644500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_031714
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 26298486-26300179  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os06g0644500						
					
						Parent gene annotation
						
							Ankyrin domain containing protein. (Os06t0644500-01);Ankyrin dom
ain containing protein. (Os06t0644500-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0644500_circ_g.1 Os06g0644500_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0644500-01:5
Os06t0644500-02:5

General Information
CircRNA Name	Os06g0644500_circ_g.2
ID in PlantcircBase	osa_circ_031714
Alias	NA
Organism	Oryza sativa
Position	chr6: 26298486-26300179 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os06g0644500
Parent gene annotation	Ankyrin domain containing protein. (Os06t0644500-01);Ankyrin dom ain containing protein. (Os06t0644500-02)
Parent gene strand	+
Alternative splicing	Os06g0644500_circ_g.1 Os06g0644500_circ_g.3
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0644500-01:5 Os06t0644500-02:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTCAATATATCATGGATATGACAGCACCATTTGGGCTGTTTGCATGGTCAGGAGTTTTCCTTGC
AACTGCTGGCCTAGTCATGTTCTATAAATGTAGCAGgcaacacatgttgcagcgcagtatggcc
agacagcatttatttatcatattgttgcgaagtggaatgctgatccagatgtccctgataatga
tggaagaa

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GCAACACATGTTGCAGCGCAGTATGGCCAGACAGCATTTATTTATCATATTGTTGCGAAGTGGA
ATGCTGATCCAGATGTCCCTGATAATGATGGAAGAAGCCCACTGCACTGGTTTGTACCTTGTTC
TCTACATTTATCATTATCTTATGTTGCACTGGAGTAGTAGTTTCTACCAGTGCACTTAGTAATG
CATGTCCCCATAAACCTTCAGTGCCCCTTATATTTCTTTCTCTCTCTCTCTGTTTTTTTTTGGC
ATATTACCTTGTAGGGCTGCTTACAAGGGATTTGCGGATTCTATACGTCTCCTTTTGTATTTGG
ATGCTTATAGGGGGCGGCAGGATAAAGAAGGCATGTGCTAAATTTATCTATTTCCATTATTACC
TGTACTGCTGTGCACATAACAGTGTAAACCAATGGCTTAGCCATATCTCCAATTATATCAGTAC
ACTTCAGAGCATGAGATTTACTTCTTTACTACAATGCTGCCCCTAGGTTGTACCCCTTTACATT
GGGCTGCTATTAGGGGGAACCTGGAGGCATGCACTGTGCTAGTTCAGGCTGGCAAAAAGGATGA
CTTGATGGTGAAGGATAAGACTGGTCTAACTCCAGCACAGCTTGCTGCTGATAAGAGTCATCGG
CAAGTTGCATTTTTTCTTGTAAGTAACATGAACCATGGCATTTCTGTTTTTGTATTATAGCCAT
TCTTTTGTAGAGCCCAATGCATTAAGCCATTAACAGTTCATCTACTAGAGATTCTGCCAAGTTA
CTTAATATGATTTGCGTAATGATGTTTACTAATAGGAAACATTTTTCTTCATCATTTTAGGTTT
TATATTCCTTCTGACTTATAAATTCTTTAGAGCTGAGTTTCATGTCTCCATGATGAGGTGTTAT
TGGTGTAATTCACTGATTATGCATAATTGCATACATAAGCTTTCTGAACTTTGTAGTTTCCCTG
TAGATTCAGTACAAGAAGCTGCAAAATTCTATGACTTCTACACATGGAAATGATATTATTGACC
AGAAATGTCATAAACTAGCATAAGACATAAAACTATGTAACCAAGGAGGCAAAATGCTACTCTT
TGTTGTGACTTTGCTGATGCTTATATGCTCTTATATATTTTTATTATAGATTCGTTGTGAGTTT
GGGAACCGAAATGATTTCCTGACTTTTTGTGATTGCACATGTAGATTAATATCCTTTTCACTAA
TTCATTATTGTTAATCTAGCTAATGTCATAAGAGTGGTCATATTGACCTTGAGGTGAAAGAATA
TTACTGGTATTTCAAGAGAGAATCTACTGGAAGATGATCCTTTTGATGATGCTTGTTTATTTAG
ACAGAAACAAGACTTGATTTGATGATTGCTTGCAGGACAATGCTAAAAGGGTATATGATAGAGG
ATGTGATGGGAATACCAATTTTGGAAAGCTATCGAAACTGGGGCTAGCTCCTGTACTTTGGTGT
ATTATTGTTGGCTTGCTTGCTACATATATACACTCTGTTATATCAGGTAAGTACCATTTCCAAT
GTCGCTCACTCCATTTGATCTCCCACGATGCAGTATTTACTACATTTCATTCTTTCAGGTCAAT
ATATCATGGATATGACAGCACCATTTGGGCTGTTTGCATGGTCAGGAGTTTTCCTTGCAACTGC
TGGCCTAGTCATGTTCTATAAATGTAGCAG
						
					

			
		

Sequences
Splice junction sequence	GTCAATATATCATGGATATGACAGCACCATTTGGGCTGTTTGCATGGTCAGGAGTTTTCCTTGC AACTGCTGGCCTAGTCATGTTCTATAAATGTAGCAGgcaacacatgttgcagcgcagtatggcc agacagcatttatttatcatattgttgcgaagtggaatgctgatccagatgtccctgataatga tggaagaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GCAACACATGTTGCAGCGCAGTATGGCCAGACAGCATTTATTTATCATATTGTTGCGAAGTGGA ATGCTGATCCAGATGTCCCTGATAATGATGGAAGAAGCCCACTGCACTGGTTTGTACCTTGTTC TCTACATTTATCATTATCTTATGTTGCACTGGAGTAGTAGTTTCTACCAGTGCACTTAGTAATG CATGTCCCCATAAACCTTCAGTGCCCCTTATATTTCTTTCTCTCTCTCTCTGTTTTTTTTTGGC ATATTACCTTGTAGGGCTGCTTACAAGGGATTTGCGGATTCTATACGTCTCCTTTTGTATTTGG ATGCTTATAGGGGGCGGCAGGATAAAGAAGGCATGTGCTAAATTTATCTATTTCCATTATTACC TGTACTGCTGTGCACATAACAGTGTAAACCAATGGCTTAGCCATATCTCCAATTATATCAGTAC ACTTCAGAGCATGAGATTTACTTCTTTACTACAATGCTGCCCCTAGGTTGTACCCCTTTACATT GGGCTGCTATTAGGGGGAACCTGGAGGCATGCACTGTGCTAGTTCAGGCTGGCAAAAAGGATGA CTTGATGGTGAAGGATAAGACTGGTCTAACTCCAGCACAGCTTGCTGCTGATAAGAGTCATCGG CAAGTTGCATTTTTTCTTGTAAGTAACATGAACCATGGCATTTCTGTTTTTGTATTATAGCCAT TCTTTTGTAGAGCCCAATGCATTAAGCCATTAACAGTTCATCTACTAGAGATTCTGCCAAGTTA CTTAATATGATTTGCGTAATGATGTTTACTAATAGGAAACATTTTTCTTCATCATTTTAGGTTT TATATTCCTTCTGACTTATAAATTCTTTAGAGCTGAGTTTCATGTCTCCATGATGAGGTGTTAT TGGTGTAATTCACTGATTATGCATAATTGCATACATAAGCTTTCTGAACTTTGTAGTTTCCCTG TAGATTCAGTACAAGAAGCTGCAAAATTCTATGACTTCTACACATGGAAATGATATTATTGACC AGAAATGTCATAAACTAGCATAAGACATAAAACTATGTAACCAAGGAGGCAAAATGCTACTCTT TGTTGTGACTTTGCTGATGCTTATATGCTCTTATATATTTTTATTATAGATTCGTTGTGAGTTT GGGAACCGAAATGATTTCCTGACTTTTTGTGATTGCACATGTAGATTAATATCCTTTTCACTAA TTCATTATTGTTAATCTAGCTAATGTCATAAGAGTGGTCATATTGACCTTGAGGTGAAAGAATA TTACTGGTATTTCAAGAGAGAATCTACTGGAAGATGATCCTTTTGATGATGCTTGTTTATTTAG ACAGAAACAAGACTTGATTTGATGATTGCTTGCAGGACAATGCTAAAAGGGTATATGATAGAGG ATGTGATGGGAATACCAATTTTGGAAAGCTATCGAAACTGGGGCTAGCTCCTGTACTTTGGTGT ATTATTGTTGGCTTGCTTGCTACATATATACACTCTGTTATATCAGGTAAGTACCATTTCCAAT GTCGCTCACTCCATTTGATCTCCCACGATGCAGTATTTACTACATTTCATTCTTTCAGGTCAAT ATATCATGGATATGACAGCACCATTTGGGCTGTTTGCATGGTCAGGAGTTTTCCTTGCAACTGC TGGCCTAGTCATGTTCTATAAATGTAGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.155417695

Conservation Information
Conserved circRNAs	NA
PMCS	0.155417695

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26299067-26299036(+)
26298524-26300156(-)
26298535-26300167(-)
						
						Potential amino acid sequence
						
							MVKDKTGLTPAQLAADKSHRQVAFFLDNAKRVYDRGCDGNTNFGKLSKLGLAPVLWCIIVGLLA
TYIHSVISGQYIMDMTAPFGLFAWSGVFLATAGLVMFYKCSRQHMLQRSMARQHLFIILLRSGM
LIQMSLIMMEEAHCTGLLTRDLRILYVSFCIWMLIGGGRIKKVVPLYIGLLLGGTWRHALC*(+
)
MLSGHTALQHVLPATFIEHD*(-)
MINKCCLAILRCNMCCLLHL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26299067-26299036(+) 26298524-26300156(-) 26298535-26300167(-)
Potential amino acid sequence	MVKDKTGLTPAQLAADKSHRQVAFFLDNAKRVYDRGCDGNTNFGKLSKLGLAPVLWCIIVGLLA TYIHSVISGQYIMDMTAPFGLFAWSGVFLATAGLVMFYKCSRQHMLQRSMARQHLFIILLRSGM LIQMSLIMMEEAHCTGLLTRDLRILYVSFCIWMLIGGGRIKKVVPLYIGLLLGGTWRHALC(+ ) MLSGHTALQHVLPATFIEHD(-) MINKCCLAILRCNMCCLLHL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0644500_circ_g.2

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