Plant Circular RNA Database

Detail information of AT5G55310_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT5G55310_circ_g.4
					
						ID in PlantcircBase
						ath_circ_044227
					
						Alias
						At_ciR2721
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 22431752-22432564  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, find_circ, CIRI-full
					
						Parent gene
						
							AT5G55310						
					
						Parent gene annotation
						
							DNA topoisomerase 1 beta
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G55310_circ_g.2 AT5G55310_circ_g.3 AT5G55310_circ_g.5 						
					
						Support reads
						3/1
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G55310.1:4

General Information
CircRNA Name	AT5G55310_circ_g.4
ID in PlantcircBase	ath_circ_044227
Alias	At_ciR2721
Organism	Arabidpsis thaliana
Position	chr5: 22431752-22432564 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, find_circ, CIRI-full
Parent gene	AT5G55310
Parent gene annotation	DNA topoisomerase 1 beta
Parent gene strand	-
Alternative splicing	AT5G55310_circ_g.2 AT5G55310_circ_g.3 AT5G55310_circ_g.5
Support reads	3/1
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G55310.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AAGACCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAATGTTGATTGTAAT
ATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCATctttaacttatttggaggaatacactcc
acgtttcctactttcaatgtacaacaaccaacagtatctgcctcatcgtcatcctatacaataa
aaataaga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 176
Transcript exons: 22431752-22432564
CTTTAACTTATTTGGAGGAATACACTCCACGTTTCCTACTTTCAATGTACAACAACCAACAGTA
TCTGCCTCATCGTCATCCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAAT
GTTGATTGTAATATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCAT
						
						Genomic sequence
						
							CTTTAACTTATTTGGAGGAATACACTCCACGTTTCCTACTTTCAATGTACAACAACCAACAGTA
TCTGCCTCATCGTCATCCTATACAATAAAAATAAGAAAGGAAAATGTGAGAGAATTGCTTGAAA
TTCCAGAAACATGTTCTATTATGAGTAAGAAGCGGGTCTTATGGAGTAATGATCTCTTATAAGC
CAAAACCAATGAGAAAATGAATAGAACGAAGGATCTCTTTAATTAAAGAAGAATCTTTGTTAAC
TTACCTTCTCATTTCCAGCCCTAAGGGCTAGCTTATCGATGAGATATGTTGCCACTGCTATTTG
CCGCTTTGTTACATCCTTGTTATTAAAATCTTTGGTGTATGCTGCTCTAATACTCCCTATATGG
TTCTGCAAACAGATGGTATGTTGCTAGGTAAGAAATGGGAAAGCAGGATGGTTTCCAGAAAAGC
TTTAAACGGCATCAAAAGCTCATTACATGTAGCTTCCTCGCTTTCTCATACTTCTCTTTGTCGC
TTTGTCCCTTCAACGAACTGCTAGCTGCCAAGAATACATACTTGAACTCTTTTGGATTGATAGG
ATCACTCCAAAAAGCAAGCCAGGTAACAGTATTGTCATGCTTAACTTCTTTCCATCTGAAAAAG
AACAGCACTCTTAGAATCAGGTCATAGGAAAAAACAAAACATGTGTCTTTTTCAAAGGGAAGCA
TATAAAAACAAGACCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAATGTT
GATTGTAATATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCAT

Sequences
Splice junction sequence	AAGACCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAATGTTGATTGTAAT ATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCATctttaacttatttggaggaatacactcc acgtttcctactttcaatgtacaacaaccaacagtatctgcctcatcgtcatcctatacaataa aaataaga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 176 Transcript exons: 22431752-22432564 CTTTAACTTATTTGGAGGAATACACTCCACGTTTCCTACTTTCAATGTACAACAACCAACAGTA TCTGCCTCATCGTCATCCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAAT GTTGATTGTAATATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCAT
Genomic sequence	CTTTAACTTATTTGGAGGAATACACTCCACGTTTCCTACTTTCAATGTACAACAACCAACAGTA TCTGCCTCATCGTCATCCTATACAATAAAAATAAGAAAGGAAAATGTGAGAGAATTGCTTGAAA TTCCAGAAACATGTTCTATTATGAGTAAGAAGCGGGTCTTATGGAGTAATGATCTCTTATAAGC CAAAACCAATGAGAAAATGAATAGAACGAAGGATCTCTTTAATTAAAGAAGAATCTTTGTTAAC TTACCTTCTCATTTCCAGCCCTAAGGGCTAGCTTATCGATGAGATATGTTGCCACTGCTATTTG CCGCTTTGTTACATCCTTGTTATTAAAATCTTTGGTGTATGCTGCTCTAATACTCCCTATATGG TTCTGCAAACAGATGGTATGTTGCTAGGTAAGAAATGGGAAAGCAGGATGGTTTCCAGAAAAGC TTTAAACGGCATCAAAAGCTCATTACATGTAGCTTCCTCGCTTTCTCATACTTCTCTTTGTCGC TTTGTCCCTTCAACGAACTGCTAGCTGCCAAGAATACATACTTGAACTCTTTTGGATTGATAGG ATCACTCCAAAAAGCAAGCCAGGTAACAGTATTGTCATGCTTAACTTCTTTCCATCTGAAAAAG AACAGCACTCTTAGAATCAGGTCATAGGAAAAAACAAAACATGTGTCTTTTTCAAAGGGAAGCA TATAAAAACAAGACCTTTCACCAGGGATAGGGCATTCTGGAATAGGCGCCTCTTTACCAATGTT GATTGTAATATCACAAGGACGAATCCGCTTTTTCAGTTTCCCCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.188348875

Conservation Information
Conserved circRNAs	NA
PMCS	0.188348875

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							22432565-22431754(-)
22432046-22432561(+)
						
						Potential amino acid sequence
						
							MGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAA
SSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDD
DEADTVGCCTLKVGNVECIPPNKLKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHD
NTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDV
TKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKMGKLKKRIRPCDIT
INIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYE
KARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVG
NVECIPPNKLKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN
PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDK
LALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLK(-)
MRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAAKNTYLNSFGLI
GSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFPIFNLFGGIHSTFPT
FNVQQPTVSASSSSFSFPALRASLSMRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSY
FSLSLCPFNELLAAKNTYLNSFGLIGSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVI
SQGRIRFFSFPIFNLFGGIHSTFPTFNVQQPTVSASSSSFSFPALRASLSMRYVATAICRFVTS
LLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAAKNTYLNSFGLIGSLQKASQVTVLSC
LTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFPIFNLFGGIHSTFPTFNVQQPTVSASSSS
FSFPALRASLSMRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAA
KNTYLNSFGLIGSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFP(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	22432565-22431754(-) 22432046-22432561(+)
Potential amino acid sequence	MGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAA SSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDD DEADTVGCCTLKVGNVECIPPNKLKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHD NTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDV TKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKMGKLKKRIRPCDIT INIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYE KARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVG NVECIPPNKLKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDK LALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLK(-) MRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAAKNTYLNSFGLI GSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFPIFNLFGGIHSTFPT FNVQQPTVSASSSSFSFPALRASLSMRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSY FSLSLCPFNELLAAKNTYLNSFGLIGSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVI SQGRIRFFSFPIFNLFGGIHSTFPTFNVQQPTVSASSSSFSFPALRASLSMRYVATAICRFVTS LLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAAKNTYLNSFGLIGSLQKASQVTVLSC LTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFPIFNLFGGIHSTFPTFNVQQPTVSASSSS FSFPALRASLSMRYVATAICRFVTSLLLKSLVYAALILPIWLCSFLAFSYFSLSLCPFNELLAA KNTYLNSFGLIGSLQKASQVTVLSCLTSFHLSPGIGHSGIGASLPMLIVISQGRIRFFSFP(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of AT5G55310_circ_g.4

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