Plant Circular RNA Database

Detail information of AT4G16660_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT4G16660_circ_g.5
					
						ID in PlantcircBase
						ath_circ_031248
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 9377179-9378719  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT4G16660						
					
						Parent gene annotation
						
							heat shock protein 70 (Hsp 70) family protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G16660_circ_g.2 AT4G16660_circ_g.3 AT4G16660_circ_g.4 AT4G16660_circ_g.6 AT4G16660_circ_g.7 AT4G16660_circ_g.8 AT4G16660_circ_g.9 AT4G16660_circ_g.10 AT4G16660_circ_g.11 AT4G16660_circ_g.12 AT4G16660_circ_g.13 AT4G16660_circ_g.14 AT4G16660_circ_g.15 AT4G16660_circ_g.16 AT4G16660_circ_g.17 AT4G16660_circ_g.18 AT4G16660_circ_g.19 						
					
						Support reads
						6
					
						Tissues
						seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G16660.1:3
AT4G16660.2:3

General Information
CircRNA Name	AT4G16660_circ_g.5
ID in PlantcircBase	ath_circ_031248
Alias	NA
Organism	Arabidpsis thaliana
Position	chr4: 9377179-9378719 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	AT4G16660
Parent gene annotation	heat shock protein 70 (Hsp 70) family protein
Parent gene strand	+
Alternative splicing	AT4G16660_circ_g.2 AT4G16660_circ_g.3 AT4G16660_circ_g.4 AT4G16660_circ_g.6 AT4G16660_circ_g.7 AT4G16660_circ_g.8 AT4G16660_circ_g.9 AT4G16660_circ_g.10 AT4G16660_circ_g.11 AT4G16660_circ_g.12 AT4G16660_circ_g.13 AT4G16660_circ_g.14 AT4G16660_circ_g.15 AT4G16660_circ_g.16 AT4G16660_circ_g.17 AT4G16660_circ_g.18 AT4G16660_circ_g.19
Support reads	6
Tissues	seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G16660.1:3 AT4G16660.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: CAGCCTCCTCGCCTAGTAAC
 R: ACTGCGGCTCCAATATCTGTG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: CAGCCTCCTCGCCTAGTAAC R: ACTGCGGCTCCAATATCTGTG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TACACCTTTAAAAGATGTGCTCAAGCATTCAGGTTTGAAGATTGATGATATATCTGCAGTGGAA
CTAATAGGAGGAGCTACTAGAGTTCCCAAACTACAGaggaattgctgctctctactcgcggtgt
ttagtttacgattggaatatggggaagatatttagctggttagtggtgttattgtcattgatct
cattagtc

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							AGGAATTGCTGCTCTCTACTCGCGGTGTTTAGTTTACGATTGGAATATGGGGAAGATATTTAGC
TGGTTAGTGGTGTTATTGTCATTGATCTCATTAGTCCCTGTTCCATCGGAATCGGCTGTTTTGA
GTGTAGATCTAGGTTCAGAATGGGTTAAAGTTGCCGTAGTTAATCTGAAACGAGGACAGAGTCC
AATCTCTGTAGCCATTAATGAGATGTCCAAGAGGAAATCCCCAGCTTTAGTAGCATTTCAATCT
GGAGACCGGTTACTAGGCGAGGAGGCTGCGGGAATAACGGCTCGTTATCCGAATAAAGTTTATT
CACAACTGAGGGATATGGTGGGAAAACCTTTCAAACATGTTAAGGATTTCATTGATTCTGTCTA
CTTGCCTTTTGATATTGTAGAAGATTCTAGAGGTGCAGTTGGTATTAAGATCGATGACGGTTCG
ACTGTTTACTCAGTGGAGGAGTTGTTAGCTATGATCTTGGGTTATGCCTCAAATCTAGCTGAGT
TCCATGCTAAAATCCCAGTGAAGGATATGGTTGTTTCAGTTCCACCTTACTTTGGACAGGCTGA
GAGACGGGGTTTGATCCAGGCTTCTCAGTTGGCTGGGGTTAATGTCCTCTCCCTGGTTAATGAG
CATTCCGGTGCTGCTTTGCAATATGGGATTGATAAGGATTTTGCTAATGGGTCGAGACATGTCA
TATTTTATGACATGGGTTCAAGTAGTACTTACGCAGCCCTTGTCTATTACTCAGCTTACAGTGA
GAAGGAATATGGCAAGACTGTTTCTGTCAACCAATTTCAGGTTAATAATGCTCTTCTATTATAT
GACTCGGATTTTTTTGAGATATATTGTATTTTGTGGTTTCTTCATTTTTTCCTTCTTGGAACAT
GTGTGACACAAGCTCAGCGTACTAATTTGTATTCGTTTGGTCTGCTTTCAGTTGGAATAGATTT
TCAATATGGGTCATGATATATTTATGTTAATGTAGGTCAAGGATGTTAGATGGGACTTGGGACT
TGGAGGACAGAGTATGGAGATGCGCTTGGTAGAGCACTTTGCAGATGAGTTTAATAAACAGCTC
GGTAATGGAGTTGATGTGAGGAAGTTCCCCAAAGCAATGGCTAAATTGAAAAAACAAGTCAAGC
GTACAAAGGAAATTTTGAGTGCAAACACTGCGGCTCCAATATCTGTGGAATCTCTTCATGATGA
TCGTGACTTCAGGTATAACTACAATATTTGGGTTTCTCTATTTCCCCAGCACATCGAAAATTGG
CTGCAAAAGTGTTATAATGATGTCTCATGCTCTAAATTGCATCATGATTGGTCTAATTTTTGTG
ATGTGCGTGATATTCTAACCTACTTATCTTTTTTCAGGAGCACAATTACCCGTGAGAAGTTTGA
GGAACTGTGTAAAGATCTGTGGGAGAGATCTCTTACACCTTTAAAAGATGTGCTCAAGCATTCA
GGTTTGAAGATTGATGATATATCTGCAGTGGAACTAATAGGAGGAGCTACTAGAGTTCCCAAAC
TACAG
						
					

			
		

Sequences
Splice junction sequence	TACACCTTTAAAAGATGTGCTCAAGCATTCAGGTTTGAAGATTGATGATATATCTGCAGTGGAA CTAATAGGAGGAGCTACTAGAGTTCCCAAACTACAGaggaattgctgctctctactcgcggtgt ttagtttacgattggaatatggggaagatatttagctggttagtggtgttattgtcattgatct cattagtc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	AGGAATTGCTGCTCTCTACTCGCGGTGTTTAGTTTACGATTGGAATATGGGGAAGATATTTAGC TGGTTAGTGGTGTTATTGTCATTGATCTCATTAGTCCCTGTTCCATCGGAATCGGCTGTTTTGA GTGTAGATCTAGGTTCAGAATGGGTTAAAGTTGCCGTAGTTAATCTGAAACGAGGACAGAGTCC AATCTCTGTAGCCATTAATGAGATGTCCAAGAGGAAATCCCCAGCTTTAGTAGCATTTCAATCT GGAGACCGGTTACTAGGCGAGGAGGCTGCGGGAATAACGGCTCGTTATCCGAATAAAGTTTATT CACAACTGAGGGATATGGTGGGAAAACCTTTCAAACATGTTAAGGATTTCATTGATTCTGTCTA CTTGCCTTTTGATATTGTAGAAGATTCTAGAGGTGCAGTTGGTATTAAGATCGATGACGGTTCG ACTGTTTACTCAGTGGAGGAGTTGTTAGCTATGATCTTGGGTTATGCCTCAAATCTAGCTGAGT TCCATGCTAAAATCCCAGTGAAGGATATGGTTGTTTCAGTTCCACCTTACTTTGGACAGGCTGA GAGACGGGGTTTGATCCAGGCTTCTCAGTTGGCTGGGGTTAATGTCCTCTCCCTGGTTAATGAG CATTCCGGTGCTGCTTTGCAATATGGGATTGATAAGGATTTTGCTAATGGGTCGAGACATGTCA TATTTTATGACATGGGTTCAAGTAGTACTTACGCAGCCCTTGTCTATTACTCAGCTTACAGTGA GAAGGAATATGGCAAGACTGTTTCTGTCAACCAATTTCAGGTTAATAATGCTCTTCTATTATAT GACTCGGATTTTTTTGAGATATATTGTATTTTGTGGTTTCTTCATTTTTTCCTTCTTGGAACAT GTGTGACACAAGCTCAGCGTACTAATTTGTATTCGTTTGGTCTGCTTTCAGTTGGAATAGATTT TCAATATGGGTCATGATATATTTATGTTAATGTAGGTCAAGGATGTTAGATGGGACTTGGGACT TGGAGGACAGAGTATGGAGATGCGCTTGGTAGAGCACTTTGCAGATGAGTTTAATAAACAGCTC GGTAATGGAGTTGATGTGAGGAAGTTCCCCAAAGCAATGGCTAAATTGAAAAAACAAGTCAAGC GTACAAAGGAAATTTTGAGTGCAAACACTGCGGCTCCAATATCTGTGGAATCTCTTCATGATGA TCGTGACTTCAGGTATAACTACAATATTTGGGTTTCTCTATTTCCCCAGCACATCGAAAATTGG CTGCAAAAGTGTTATAATGATGTCTCATGCTCTAAATTGCATCATGATTGGTCTAATTTTTGTG ATGTGCGTGATATTCTAACCTACTTATCTTTTTTCAGGAGCACAATTACCCGTGAGAAGTTTGA GGAACTGTGTAAAGATCTGTGGGAGAGATCTCTTACACCTTTAAAAGATGTGCTCAAGCATTCA GGTTTGAAGATTGATGATATATCTGCAGTGGAACTAATAGGAGGAGCTACTAGAGTTCCCAAAC TACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.177841525

Conservation Information
Conserved circRNAs	NA
PMCS	0.177841525

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT4G16660_circ_g.5

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