Plant Circular RNA Database

Detail information of Csa2G076520_circ_g.2

			
						 General Information
					
						CircRNA Name
						Csa2G076520_circ_g.2
					
						ID in PlantcircBase
						csa_circ_000911
					
						Alias
						Chr2:6337912_6345904
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr2: 6337912-6345904  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2;Find_circ
					
						Parent gene
						
							Csa2G076520						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Csa2G076520_circ_g.3 Csa2G076520_circ_g.4 Csa2G076520_circ_g.5 Csa2G076520_circ_g.6 Csa2G076520_circ_g.7 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa2G076520.1:4

General Information
CircRNA Name	Csa2G076520_circ_g.2
ID in PlantcircBase	csa_circ_000911
Alias	Chr2:6337912_6345904
Organism	Cucumis sativus
Position	chrChr2: 6337912-6345904 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2;Find_circ
Parent gene	Csa2G076520
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Csa2G076520_circ_g.3 Csa2G076520_circ_g.4 Csa2G076520_circ_g.5 Csa2G076520_circ_g.6 Csa2G076520_circ_g.7
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa2G076520.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCTCTTTTTCCAAGCTTCAGTAAAGCAACCATTAGAAGCTTGACTTTCAATTAGCTTGTAGAG
ACTTGGCAGCTCAATACCAGCACCATCAAGTTGCTCctgtttacatcttttctgatttcgcttt
tgtttcaaatgtatttggagttttcggtcaaaaatggcatcatcttcttctttgacctgtcaat
acaaagta

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 636
Transcript exons: 6337912-6337995,6341594-6341707,6345373-6345619,6345714-
6345904
CTGTTTACATCTTTTCTGATTTCGCTTTTGTTTCAAATGTATTTGGAGTTTTCGGTCAAAAATG
GCATCATCTTCTTCTTTGACCTTTCTGAAATTTTTCTTTTGTTCTTCAGAAAGATCCAACTCTT
TTTCATTTTCAATGGCAGCTGCAACAAATGGATCACAAGAACTTCCATCAACATCATCTATCTC
CTCTACCTCATCAACTCCAGGAAATTTGAGTCCCATTTCTGCAGCTTGTTGCGGAGTGCTAGCC
AAGTAAACAGAAGCCCAGTTCTTGCTGCCAAACAACGTGTCACTGTCCTTTGAGCCTTCTGAAA
AAACCTTATTAAGAGAGCCCCAATCAACTTCCAATTTTTCTGTACCGATTCCCTCAATCTCGTG
AGTGGAAAATTTCTTCTGCAGATATCCACTTGCTCCCTCCTCCAACAGCTTACCATGTCGTCTC
ATTACAGGCCTTTCAATTTGGAGGGTTTTCTCTGCGTCAGATACAGATGCAAGAAGATCACCAG
TTACCTGAGACCCTACCCAGTGTATCCTCTTTTTCCAAGCTTCAGTAAAGCAACCATTAGAAGC
TTGACTTTCAATTAGCTTGTAGAGACTTGGCAGCTCAATACCAGCACCATCAAGTTGCTC
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	TCCTCTTTTTCCAAGCTTCAGTAAAGCAACCATTAGAAGCTTGACTTTCAATTAGCTTGTAGAG ACTTGGCAGCTCAATACCAGCACCATCAAGTTGCTCctgtttacatcttttctgatttcgcttt tgtttcaaatgtatttggagttttcggtcaaaaatggcatcatcttcttctttgacctgtcaat acaaagta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 636 Transcript exons: 6337912-6337995,6341594-6341707,6345373-6345619,6345714- 6345904 CTGTTTACATCTTTTCTGATTTCGCTTTTGTTTCAAATGTATTTGGAGTTTTCGGTCAAAAATG GCATCATCTTCTTCTTTGACCTTTCTGAAATTTTTCTTTTGTTCTTCAGAAAGATCCAACTCTT TTTCATTTTCAATGGCAGCTGCAACAAATGGATCACAAGAACTTCCATCAACATCATCTATCTC CTCTACCTCATCAACTCCAGGAAATTTGAGTCCCATTTCTGCAGCTTGTTGCGGAGTGCTAGCC AAGTAAACAGAAGCCCAGTTCTTGCTGCCAAACAACGTGTCACTGTCCTTTGAGCCTTCTGAAA AAACCTTATTAAGAGAGCCCCAATCAACTTCCAATTTTTCTGTACCGATTCCCTCAATCTCGTG AGTGGAAAATTTCTTCTGCAGATATCCACTTGCTCCCTCCTCCAACAGCTTACCATGTCGTCTC ATTACAGGCCTTTCAATTTGGAGGGTTTTCTCTGCGTCAGATACAGATGCAAGAAGATCACCAG TTACCTGAGACCCTACCCAGTGTATCCTCTTTTTCCAAGCTTCAGTAAAGCAACCATTAGAAGC TTGACTTTCAATTAGCTTGTAGAGACTTGGCAGCTCAATACCAGCACCATCAAGTTGCTC
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.828721479

Conservation Information
Conserved circRNAs	NA
PMCS	0.828721479

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6345720-6337914(-)
						
						Potential amino acid sequence
						
							MRRHGKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVY
LASTPQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDD
AIFDRKLQIHLKQKRNQKRCKQEQLDGAGIELPSLYKLIESQASNGCFTEAWKKRIHWVGSQVT
GDLLASVSDAEKTLQIERPVMRRHGKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVF
SEGSKDSDTLFGSKNWASVYLASTPQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEK
ELDLSEEQKKNFRKVKEEDDAIFDRKLQIHLKQKRNQKRCKQEQLDGAGIELPSLYKLIESQAS
NGCFTEAWKKRIHWVGSQVTGDLLASVSDAEKTLQIERPVMRRHGKLLEEGASGYLQKKFSTHE
IEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVYLASTPQQAAEMGLKFPGVDEVEEI
DDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDDAIFDRKLQIHLKQKRNQKRCKQEQ
LDGAGIELPSLYKLIESQASNGCFTEAWKKRIHWVGSQVTGDLLASVSDAEKTLQIERPVMRRH
GKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVYLAST
PQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDDAIFD
RKLQIHLKQKRNQKRCKQ
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root, responsive to salt stress in leaf

Functional Information
Coding potential	Y
Potential coding position	6345720-6337914(-)
Potential amino acid sequence	MRRHGKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVY LASTPQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDD AIFDRKLQIHLKQKRNQKRCKQEQLDGAGIELPSLYKLIESQASNGCFTEAWKKRIHWVGSQVT GDLLASVSDAEKTLQIERPVMRRHGKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVF SEGSKDSDTLFGSKNWASVYLASTPQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEK ELDLSEEQKKNFRKVKEEDDAIFDRKLQIHLKQKRNQKRCKQEQLDGAGIELPSLYKLIESQAS NGCFTEAWKKRIHWVGSQVTGDLLASVSDAEKTLQIERPVMRRHGKLLEEGASGYLQKKFSTHE IEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVYLASTPQQAAEMGLKFPGVDEVEEI DDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDDAIFDRKLQIHLKQKRNQKRCKQEQ LDGAGIELPSLYKLIESQASNGCFTEAWKKRIHWVGSQVTGDLLASVSDAEKTLQIERPVMRRH GKLLEEGASGYLQKKFSTHEIEGIGTEKLEVDWGSLNKVFSEGSKDSDTLFGSKNWASVYLAST PQQAAEMGLKFPGVDEVEEIDDVDGSSCDPFVAAAIENEKELDLSEEQKKNFRKVKEEDDAIFD RKLQIHLKQKRNQKRCKQ
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root, responsive to salt stress in leaf

			
						 Other Information
					
						References
						Zhu et al., 2019;He et al., 2020

circRNA

Detail information of Csa2G076520_circ_g.2

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