Plant Circular RNA Database

Detail information of orai.002G201600_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.002G201600_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000193
					
						Alias
						Chr02:54286671|54288307
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr02: 54286671-54288307  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.002G201600						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.002G201600.2:5
Gorai.002G201600.1:5

General Information
CircRNA Name	orai.002G201600_circ_g.1
ID in PlantcircBase	gra_circ_000193
Alias	Chr02:54286671\|54288307
Organism	Gossypium raimondii
Position	chrChr02: 54286671-54288307 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.002G201600
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.002G201600.2:5 Gorai.002G201600.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTGCTTCTTGTGAGTGCAACTGCTTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCTG
TTCGTAAGAAGTCACGTTCAGCAACATTATCACCTCcttcaaaaggcaaacagatttgatctta
gacagataaacatccatattgctatttgcagtaaatcggtgaacaacagtttgaactaatcttt
ggcacttc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 845
Transcript exons: 54286671-54286859,54287262-54287522,54287596-54287701,54
287843-54288030,54288207-54288307
CTTCAAAAGGCAAACAGATTTGATCTTAGACAGATAAACATCCATATTGCTATTTGCAGTAAAT
CGGTGAACAACAGTTTGAACTAATCTTTGGCACTTCATTATAGCATTCACACCCATTGGGGAAT
GTCTTGCTATTGCAACTAGTACTGAAATCAAGCATTCTTCCAAAGGTGCAGTTGGTTCTATCTC
CAAAAGATATCGAATCCTCGGAAGGATTCCCATCCTAACCAAACCAGCAGCAAAATCTTGTCCA
GCAACAACAATGTCATCCTGAATTGTATGCTTCCCTTCAGTTTCATCTTCCACAATATTATCTC
CATAAAGAAGAACATTTGAAGGTTTAGCACTGTATTTCCAAAATCCACCATGAAGGAAACCAAC
ATCAATCTCTGGTTTAGACCGAAATATAGGAGCAGTATAAGTACCCCTCATGTCAATTGCTGTT
TTCTCCAAAAGATCAAAGAAACTCTGATTTATATCACAGCTCAATACGCACTGAATAACTTTGG
CGGTTGCTAAAACTACAGAATTGTGGTTATCATCAAGCGACATCCTTAATGATAAGATTAGCTC
AGGTTCTGGGCCCAGTGCAAAAGCCCAAACAGCTTCCCAGTCAACAGTGCTGTCAACATTGTCC
TTATCAGCCAGAGTAGAACCAATTGGATTGAGATAAATATTACGTAATGCCTTATCAAGCACAG
ATGCAAGGAGATGCAATGCAAGGGCCCGTTGACCTGGAATCGTGCTTCTTGTGAGTGCAACTGC
TTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCTGTTCGTAAGAAGTCACGTTCAGCA
ACATTATCACCTC
						
					

						Genomic sequence
						
							CTTCAAAAGGCAAACAGATTTGATCTTAGACAGATAAACATCCATATTGCTATTTGCAGTAAAT
CGGTGAACAACAGTTTGAACTAATCTTTGGCACTTCATTATAGCATTCACACCCATTGGGGAAT
GTCTTGCTATTGCAACTAGTACTGAAATCAAGCATTCTTCCAAAGGTGCAGTTGGTTCTATCTG
AAAACATGAGTAACGTTACTAATGTATATATCAGAAATATTTCACAAAGTAAGCATTGAACTTC
CAGGAGAGACACTATGGCTGCTCTCCTCTTAGGAGTCGGAACAACTAAAATATAGACCCACCAA
ATTAGCATAGATATTATTGTGCTACATATTAGTTTCCAGGGTCATGCAAGTATGAAGAAAGGAA
AAAGGTAAACACGAAAATCTGCAGTAGAAAAAAGAAGATGCTAGCACAAATTCTAAATCAGTAC
TAATTTCTCTTCACACGACCCACAAAAAAGACAATTGTTTAAACTAGATAACAAGTTTATTAAA
TTTGAATAAAACATACGCGGAAACCTGATGGCAGGTATATCAAGAACTTAAGTAAACATAATGG
ATTTATTCTGCTCACCTCCAAAAGATATCGAATCCTCGGAAGGATTCCCATCCTAACCAAACCA
GCAGCAAAATCTTGTCCAGCAACAACAATGTCATCCTGAATTGTATGCTTCCCTTCAGTTTCAT
CTTCCACAATATTATCTCCATAAAGAAGAACATTTGAAGGTTTAGCACTGTATTTCCAAAATCC
ACCATGAAGGAAACCAACATCAATCTCTGGTTTAGACCGAAATATAGGAGCAGTATAAGTACCC
CTCATGTCAATTGCTGTTTTCTGCATTATAGAACATTGGTTAAACTAAACATTACATATGGACA
TACAAGGCATATAAAAGATAGAAATCTACCTCCAAAAGATCAAAGAAACTCTGATTTATATCAC
AGCTCAATACGCACTGAATAACTTTGGCGGTTGCTAAAACTACAGAATTGTGGTTATCATCAAG
CGACATCCTACAGATTTCAAAATGTTCAAATTCAAGGGTAAAGAATACAGAATCAAAATCATTT
TATGAATCAACATCTAAATTTTAACAGGAAAAAAACAGTTTGACTTAACATTATTCAGTTTTTT
GGGATAAAAATTCATTTAACCTTAATGATAAGATTAGCTCAGGTTCTGGGCCCAGTGCAAAAGC
CCAAACAGCTTCCCAGTCAACAGTGCTGTCAACATTGTCCTTATCAGCCAGAGTAGAACCAATT
GGATTGAGATAAATATTACGTAATGCCTTATCAAGCACAGATGCAAGGAGATGCAATGCAAGGG
CCCGTTGACCTGGAATCTAAAGTTAACAATATCAGTAAAAACAGGAGATCAGTATAGTCATATC
AAAGATAAACTGCAACAATATTTAAGATTGACATGCACAGTCTAGCTATATCATGACAACATGC
AGCTGCATTTGCATTAAAGATCTACATGTCATCCAATAAAATAATAAATAAAATATAAACTGAC
CGTGCTTCTTGTGAGTGCAACTGCTTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCT
GTTCGTAAGAAGTCACGTTCAGCAACATTATCACCTC
						
					

			
		

Sequences
Splice junction sequence	GTGCTTCTTGTGAGTGCAACTGCTTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCTG TTCGTAAGAAGTCACGTTCAGCAACATTATCACCTCcttcaaaaggcaaacagatttgatctta gacagataaacatccatattgctatttgcagtaaatcggtgaacaacagtttgaactaatcttt ggcacttc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 845 Transcript exons: 54286671-54286859,54287262-54287522,54287596-54287701,54 287843-54288030,54288207-54288307 CTTCAAAAGGCAAACAGATTTGATCTTAGACAGATAAACATCCATATTGCTATTTGCAGTAAAT CGGTGAACAACAGTTTGAACTAATCTTTGGCACTTCATTATAGCATTCACACCCATTGGGGAAT GTCTTGCTATTGCAACTAGTACTGAAATCAAGCATTCTTCCAAAGGTGCAGTTGGTTCTATCTC CAAAAGATATCGAATCCTCGGAAGGATTCCCATCCTAACCAAACCAGCAGCAAAATCTTGTCCA GCAACAACAATGTCATCCTGAATTGTATGCTTCCCTTCAGTTTCATCTTCCACAATATTATCTC CATAAAGAAGAACATTTGAAGGTTTAGCACTGTATTTCCAAAATCCACCATGAAGGAAACCAAC ATCAATCTCTGGTTTAGACCGAAATATAGGAGCAGTATAAGTACCCCTCATGTCAATTGCTGTT TTCTCCAAAAGATCAAAGAAACTCTGATTTATATCACAGCTCAATACGCACTGAATAACTTTGG CGGTTGCTAAAACTACAGAATTGTGGTTATCATCAAGCGACATCCTTAATGATAAGATTAGCTC AGGTTCTGGGCCCAGTGCAAAAGCCCAAACAGCTTCCCAGTCAACAGTGCTGTCAACATTGTCC TTATCAGCCAGAGTAGAACCAATTGGATTGAGATAAATATTACGTAATGCCTTATCAAGCACAG ATGCAAGGAGATGCAATGCAAGGGCCCGTTGACCTGGAATCGTGCTTCTTGTGAGTGCAACTGC TTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCTGTTCGTAAGAAGTCACGTTCAGCA ACATTATCACCTC
Genomic sequence	CTTCAAAAGGCAAACAGATTTGATCTTAGACAGATAAACATCCATATTGCTATTTGCAGTAAAT CGGTGAACAACAGTTTGAACTAATCTTTGGCACTTCATTATAGCATTCACACCCATTGGGGAAT GTCTTGCTATTGCAACTAGTACTGAAATCAAGCATTCTTCCAAAGGTGCAGTTGGTTCTATCTG AAAACATGAGTAACGTTACTAATGTATATATCAGAAATATTTCACAAAGTAAGCATTGAACTTC CAGGAGAGACACTATGGCTGCTCTCCTCTTAGGAGTCGGAACAACTAAAATATAGACCCACCAA ATTAGCATAGATATTATTGTGCTACATATTAGTTTCCAGGGTCATGCAAGTATGAAGAAAGGAA AAAGGTAAACACGAAAATCTGCAGTAGAAAAAAGAAGATGCTAGCACAAATTCTAAATCAGTAC TAATTTCTCTTCACACGACCCACAAAAAAGACAATTGTTTAAACTAGATAACAAGTTTATTAAA TTTGAATAAAACATACGCGGAAACCTGATGGCAGGTATATCAAGAACTTAAGTAAACATAATGG ATTTATTCTGCTCACCTCCAAAAGATATCGAATCCTCGGAAGGATTCCCATCCTAACCAAACCA GCAGCAAAATCTTGTCCAGCAACAACAATGTCATCCTGAATTGTATGCTTCCCTTCAGTTTCAT CTTCCACAATATTATCTCCATAAAGAAGAACATTTGAAGGTTTAGCACTGTATTTCCAAAATCC ACCATGAAGGAAACCAACATCAATCTCTGGTTTAGACCGAAATATAGGAGCAGTATAAGTACCC CTCATGTCAATTGCTGTTTTCTGCATTATAGAACATTGGTTAAACTAAACATTACATATGGACA TACAAGGCATATAAAAGATAGAAATCTACCTCCAAAAGATCAAAGAAACTCTGATTTATATCAC AGCTCAATACGCACTGAATAACTTTGGCGGTTGCTAAAACTACAGAATTGTGGTTATCATCAAG CGACATCCTACAGATTTCAAAATGTTCAAATTCAAGGGTAAAGAATACAGAATCAAAATCATTT TATGAATCAACATCTAAATTTTAACAGGAAAAAAACAGTTTGACTTAACATTATTCAGTTTTTT GGGATAAAAATTCATTTAACCTTAATGATAAGATTAGCTCAGGTTCTGGGCCCAGTGCAAAAGC CCAAACAGCTTCCCAGTCAACAGTGCTGTCAACATTGTCCTTATCAGCCAGAGTAGAACCAATT GGATTGAGATAAATATTACGTAATGCCTTATCAAGCACAGATGCAAGGAGATGCAATGCAAGGG CCCGTTGACCTGGAATCTAAAGTTAACAATATCAGTAAAAACAGGAGATCAGTATAGTCATATC AAAGATAAACTGCAACAATATTTAAGATTGACATGCACAGTCTAGCTATATCATGACAACATGC AGCTGCATTTGCATTAAAGATCTACATGTCATCCAATAAAATAATAAATAAAATATAAACTGAC CGTGCTTCTTGTGAGTGCAACTGCTTCTTTAATTGTATAACCTAAAGCGCCAGGATCTCCCTCT GTTCGTAAGAAGTCACGTTCAGCAACATTATCACCTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.452973234

Conservation Information
Conserved circRNAs	NA
PMCS	0.452973234

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							54287701-54288250(-)
						
						Potential amino acid sequence
						
							MSLDDNHNSVVLATAKVIQCVLSCDINQSFFDLLEKTAIDMRGTYTAPIFRSKPEIDVGFLHGG
FWKYSAKPSNVLLYGDNIVEDETEGKHTIQDDIVVAGQDFAAGLVRMGILPRIRYLLEIEPTAP
LEECLISVLVAIARHSPMGVNAIMKCQRLVQTVVHRFTANSNMDVYLSKIKSVCLLKEVIMLLN
VTSYEQREILAL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	54287701-54288250(-)
Potential amino acid sequence	MSLDDNHNSVVLATAKVIQCVLSCDINQSFFDLLEKTAIDMRGTYTAPIFRSKPEIDVGFLHGG FWKYSAKPSNVLLYGDNIVEDETEGKHTIQDDIVVAGQDFAAGLVRMGILPRIRYLLEIEPTAP LEECLISVLVAIARHSPMGVNAIMKCQRLVQTVVHRFTANSNMDVYLSKIKSVCLLKEVIMLLN VTSYEQREILAL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.002G201600_circ_g.1

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