Plant Circular RNA Database

Detail information of Os01g0767700_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os01g0767700_circ_g.2
					
						ID in PlantcircBase
						osa_circ_004049
					
						Alias
						Os01circ24173
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 32352071-32352839  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, SMALT, Segemehl, circRNA_finder
					
						Parent gene
						
							Os01g0767700						
					
						Parent gene annotation
						
							Similar to DEIH-box RNA/DNA helicase. (Os01t0767700-01);Similar 
to predicted protein. (Os01t0767700-02);Similar to predicted pro
tein. (Os01t0767700-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0767700_circ_g.3 Os01g0767700_circ_g.4 Os01g0767700_circ_g.5 Os01g0767700_circ_g.6 Os01g0767700_circ_g.7 						
					
						Support reads
						3/3
					
						Tissues
						leaf/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0767700-01:3
Os01t0767700-02:3

General Information
CircRNA Name	Os01g0767700_circ_g.2
ID in PlantcircBase	osa_circ_004049
Alias	Os01circ24173
Organism	Oryza sativa
Position	chr1: 32352071-32352839 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, SMALT, Segemehl, circRNA_finder
Parent gene	Os01g0767700
Parent gene annotation	Similar to DEIH-box RNA/DNA helicase. (Os01t0767700-01);Similar to predicted protein. (Os01t0767700-02);Similar to predicted pro tein. (Os01t0767700-03)
Parent gene strand	+
Alternative splicing	Os01g0767700_circ_g.3 Os01g0767700_circ_g.4 Os01g0767700_circ_g.5 Os01g0767700_circ_g.6 Os01g0767700_circ_g.7
Support reads	3/3
Tissues	leaf/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0767700-01:3 Os01t0767700-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AACTTAGTGTTGTACATTTATACAGATTGTCCAAGATAGATCTAAACTTCCAATTTCCTCATAC
AAGGATGCAATTTCTTCGACTTTAGAGAATCACCAGtgtacaaatttgaacctggcatatcgaa
acaagagcgagcggcgatacatgagatgtgtagaaaaatgggcatgatatccaaaagttctggg
tgagtgat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 476
Transcript exons: 32352071-32352161,32352326-32352635,32352765-32352839
TGTACAAATTTGAACCTGGCATATCGAAACAAGAGCGAGCGGCGATACATGAGATGTGTAGAAA
AATGGGCATGATATCCAAAAGTTCTGGGAATGGGGAACGACGATGCCTATCTGTTTACAAAAGA
AAACAGAACCAGGGCCTTGAAACAGAAGAAGGACCTAGCCATCTTGGATTTTCTGTGGAAGCGA
GAAATGTTTTGCAAGATCTATTTATGCATTATCCCCCAGATGATGCGGAGCTAAATGGGCATAC
TGTCAGGAATTCTAGTGATAAGGCTGTAAAGATTCAGTGGAAGCCAGATGGTGCATTTTGTAGG
CCAGCATTGCGCAAACCGGATATATTGAAGAAGGTGGAGATGCTTGCATCGAAAGTAAATAAAT
CCGAACAGTTGAGGAAGATTGTCCAAGATAGATCTAAACTTCCAATTTCCTCATACAAGGATGC
AATTTCTTCGACTTTAGAGAATCACCAG
						
						Genomic sequence
						
							TGTACAAATTTGAACCTGGCATATCGAAACAAGAGCGAGCGGCGATACATGAGATGTGTAGAAA
AATGGGCATGATATCCAAAAGTTCTGGGTGAGTGATAGTCTAATTGACCTCTGGAATGATGCAT
TCCGTACACTGGTTCTAGTAAAGAGTAATAAGCATCTTTTAAACTTCATTCATCATAGCGACTG
TTATCTTAGTAGGATTAAACTATGTGTATCTGATGTTTTCTGAAATCTATTAATTAACTATAGG
AATGGGGAACGACGATGCCTATCTGTTTACAAAAGAAAACAGAACCAGGGCCTTGAAACAGAAG
AAGGACCTAGCCATCTTGGATTTTCTGTGGAAGCGAGAAATGTTTTGCAAGATCTATTTATGCA
TTATCCCCCAGATGATGCGGAGCTAAATGGGCATACTGTCAGGAATTCTAGTGATAAGGCTGTA
AAGATTCAGTGGAAGCCAGATGGTGCATTTTGTAGGCCAGCATTGCGCAAACCGGATATATTGA
AGAAGGTGGAGATGCTTGCATCGAAAGTAAATAAATCCGAACAGTTGAGGAAGGTTGTATATTC
TGGCAATCTGGCTTGATATGGTGTCCTTTTTGCCATTCAGTTTAGCCAATTCCTACTTTTATAT
ATGTTATATTGGATAGTTTTATCTAATTGAACTTAGTGTTGTACATTTATACAGATTGTCCAAG
ATAGATCTAAACTTCCAATTTCCTCATACAAGGATGCAATTTCTTCGACTTTAGAGAATCACCA
G

Sequences
Splice junction sequence	AACTTAGTGTTGTACATTTATACAGATTGTCCAAGATAGATCTAAACTTCCAATTTCCTCATAC AAGGATGCAATTTCTTCGACTTTAGAGAATCACCAGtgtacaaatttgaacctggcatatcgaa acaagagcgagcggcgatacatgagatgtgtagaaaaatgggcatgatatccaaaagttctggg tgagtgat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 476 Transcript exons: 32352071-32352161,32352326-32352635,32352765-32352839 TGTACAAATTTGAACCTGGCATATCGAAACAAGAGCGAGCGGCGATACATGAGATGTGTAGAAA AATGGGCATGATATCCAAAAGTTCTGGGAATGGGGAACGACGATGCCTATCTGTTTACAAAAGA AAACAGAACCAGGGCCTTGAAACAGAAGAAGGACCTAGCCATCTTGGATTTTCTGTGGAAGCGA GAAATGTTTTGCAAGATCTATTTATGCATTATCCCCCAGATGATGCGGAGCTAAATGGGCATAC TGTCAGGAATTCTAGTGATAAGGCTGTAAAGATTCAGTGGAAGCCAGATGGTGCATTTTGTAGG CCAGCATTGCGCAAACCGGATATATTGAAGAAGGTGGAGATGCTTGCATCGAAAGTAAATAAAT CCGAACAGTTGAGGAAGATTGTCCAAGATAGATCTAAACTTCCAATTTCCTCATACAAGGATGC AATTTCTTCGACTTTAGAGAATCACCAG
Genomic sequence	TGTACAAATTTGAACCTGGCATATCGAAACAAGAGCGAGCGGCGATACATGAGATGTGTAGAAA AATGGGCATGATATCCAAAAGTTCTGGGTGAGTGATAGTCTAATTGACCTCTGGAATGATGCAT TCCGTACACTGGTTCTAGTAAAGAGTAATAAGCATCTTTTAAACTTCATTCATCATAGCGACTG TTATCTTAGTAGGATTAAACTATGTGTATCTGATGTTTTCTGAAATCTATTAATTAACTATAGG AATGGGGAACGACGATGCCTATCTGTTTACAAAAGAAAACAGAACCAGGGCCTTGAAACAGAAG AAGGACCTAGCCATCTTGGATTTTCTGTGGAAGCGAGAAATGTTTTGCAAGATCTATTTATGCA TTATCCCCCAGATGATGCGGAGCTAAATGGGCATACTGTCAGGAATTCTAGTGATAAGGCTGTA AAGATTCAGTGGAAGCCAGATGGTGCATTTTGTAGGCCAGCATTGCGCAAACCGGATATATTGA AGAAGGTGGAGATGCTTGCATCGAAAGTAAATAAATCCGAACAGTTGAGGAAGGTTGTATATTC TGGCAATCTGGCTTGATATGGTGTCCTTTTTGCCATTCAGTTTAGCCAATTCCTACTTTTATAT ATGTTATATTGGATAGTTTTATCTAATTGAACTTAGTGTTGTACATTTATACAGATTGTCCAAG ATAGATCTAAACTTCCAATTTCCTCATACAAGGATGCAATTTCTTCGACTTTAGAGAATCACCA G

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_002420* osi_circ_010758 						
					
						PMCS
						0.169807607

Conservation Information
Conserved circRNAs	osi_circ_002420* osi_circ_010758
PMCS	0.169807607

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32352125-32352142(+)
32352145-32352783(-)
						
						Potential amino acid sequence
						
							MCRKMGMISKSSGNGERRCLSVYKRKQNQGLETEEGPSHLGFSVEARNVLQDLFMHYPPDDAEL
NGHTVRNSSDKAVKIQWKPDGAFCRPALRKPDILKKVEMLASKVNKSEQLRKIVQDRSKLPISS
YKDAISSTLENHQCTNLNLAYRNKSERRYMRCVEKWA*(+)
MPIFLHISCIAARSCFDMPGSNLYTGDSLKSKKLHPCMRKLEV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32352125-32352142(+) 32352145-32352783(-)
Potential amino acid sequence	MCRKMGMISKSSGNGERRCLSVYKRKQNQGLETEEGPSHLGFSVEARNVLQDLFMHYPPDDAEL NGHTVRNSSDKAVKIQWKPDGAFCRPALRKPDILKKVEMLASKVNKSEQLRKIVQDRSKLPISS YKDAISSTLENHQCTNLNLAYRNKSERRYMRCVEKWA(+) MPIFLHISCIAARSCFDMPGSNLYTGDSLKSKKLHPCMRKLEV(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0767700_circ_g.2

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