Plant Circular RNA Database

Detail information of Os03g0165400_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0165400_circ_g.4
					
						ID in PlantcircBase
						osa_circ_018092
					
						Alias
						Os_ciR4211
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 3528982-3530024  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0165400						
					
						Parent gene annotation
						
							Similar to Relative to SR12 protein (Fragment). (Os03t0165400-01
);Similar to Beta-galactosidase. (Os03t0165400-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os03g0165400_circ_g.5 						
					
						Support reads
						8/4
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0165400-01:4

General Information
CircRNA Name	Os03g0165400_circ_g.4
ID in PlantcircBase	osa_circ_018092
Alias	Os_ciR4211
Organism	Oryza sativa
Position	chr3: 3528982-3530024 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0165400
Parent gene annotation	Similar to Relative to SR12 protein (Fragment). (Os03t0165400-01 );Similar to Beta-galactosidase. (Os03t0165400-02)
Parent gene strand	-
Alternative splicing	Os03g0165400_circ_g.5
Support reads	8/4
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0165400-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AAGATCCAGAAATGCTGAAATACTTCCACAAACCTTGAAAGGTTCATTGTCCGTCCTGAAGCTG
ATGCCTGGTACATACTTCAACCAAACTGGAAATCCAcatccactccaagcttcagtccacatcg
taggcttgtaaggcttgttaggagaaaatgtgtcacaatagaaaccattgcatgcattgatctg
tttaaaga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 117
Transcript exons: 3528982-3529047,3529974-3530024
CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC
AATCCGTCCTGAAGCTGATGCCTGGTACATACTTCAACCAAACTGGAAATCCA
Transcript length: 393
Transcript exons: 3528982-3529070,3529424-3529567,3529777-3529869,3529958-
3530024
CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC
AATAGAAACCATTGCATGCATTGATCACTGGGTCAGGTGCGTCATCCTCCTTGCACATCACCCA
CGGCACACCGGTGTCCAATCCCACGGCCATCTTTGCCGCCCAGTTGATATATGCCTTGCCGGCA
GCCCCAAACTCTTTACCTTCTGGCCCATACTCGTTCTCAATCTGAGAGAGGATAATAGGACCGC
CTTGTGAAGCAAAGAGGTTTTCACTCTTCATCATGCCCACAATTTTCTCTGTGAACCCCTGCAT
TGCATTCTTGAAAGGTTCATTGTCCGTCCTGAAGCTGATGCCTGGTACATACTTCAACCAAACT
GGAAATCCA
						
					

						Genomic sequence
						
							CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC
AATAGAAACCATTGCATGCATTGATCTGTTTAAAGAACAGAGGTTAAATGTAATTTCAATAATT
TGTTCGTACAGAAGTCATGAGATATTTGTATATTCCACGACTGAAATTTCACAAGATCAAAATC
AATGTGGTTGGCGTCAATTTTTCTTCTTCTAATATATGTTTGATATATGAGTAGGCGACAAATA
TGCTCATACATATGCTAGAAACCTAGAACACCAATCATGACCTCTTATGGAGATAACCCACTGA
ACTCGCAAAAATACGAATCAGCATGTCCTGTATCGTTGTATAATTGTATTAACATGTTCGGTAT
AGTTTCACAATTTACACCTGATGGTGGTAGTATTTGACAGAGGATAAGATCATCATACCACTGG
GTCAGGTGCGTCATCCTCCTTGCACATCACCCACGGCACACCGGTGTCCAATCCCACGGCCATC
TTTGCCGCCCAGTTGATATATGCCTTGCCGGCAGCCCCAAACTCTTTACCTTCTGGCCCATACT
CGTTCTCAATCTGTGTCCAAACAATAACACCCACCGCACCATGAGCAACCCAGGAAAATGTGCA
GGCTAAGATGAATCGTGCAAACAACACATTACTCAACCACTACTACGTACTAACCCAATGTTAT
ACAATGTGCTTGCATCCATATACATGACATGGTTGAGGCCCTCTCTCTAAAAAAAATATACATG
ACATGGGAAGCAAAGTTTTGCACTTGCCTGAGAGAGGATAATAGGACCGCCTTGTGAAGCAAAG
AGGTTTTCACTCTTCATCATGCCCACAATTTTCTCTGTGAACCCCTGCATTGCATTCTGCAAAG
CAAAACTCAAGTCAGAAACACATATGAACAAGATTTACACCTCTGGTAAGATCCAGAAATGCTG
AAATACTTCCACAAACCTTGAAAGGTTCATTGTCCGTCCTGAAGCTGATGCCTGGTACATACTT
CAACCAAACTGGAAATCCA
						
					

			
		

Sequences
Splice junction sequence	AAGATCCAGAAATGCTGAAATACTTCCACAAACCTTGAAAGGTTCATTGTCCGTCCTGAAGCTG ATGCCTGGTACATACTTCAACCAAACTGGAAATCCAcatccactccaagcttcagtccacatcg taggcttgtaaggcttgttaggagaaaatgtgtcacaatagaaaccattgcatgcattgatctg tttaaaga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 117 Transcript exons: 3528982-3529047,3529974-3530024 CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC AATCCGTCCTGAAGCTGATGCCTGGTACATACTTCAACCAAACTGGAAATCCA Transcript length: 393 Transcript exons: 3528982-3529070,3529424-3529567,3529777-3529869,3529958- 3530024 CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC AATAGAAACCATTGCATGCATTGATCACTGGGTCAGGTGCGTCATCCTCCTTGCACATCACCCA CGGCACACCGGTGTCCAATCCCACGGCCATCTTTGCCGCCCAGTTGATATATGCCTTGCCGGCA GCCCCAAACTCTTTACCTTCTGGCCCATACTCGTTCTCAATCTGAGAGAGGATAATAGGACCGC CTTGTGAAGCAAAGAGGTTTTCACTCTTCATCATGCCCACAATTTTCTCTGTGAACCCCTGCAT TGCATTCTTGAAAGGTTCATTGTCCGTCCTGAAGCTGATGCCTGGTACATACTTCAACCAAACT GGAAATCCA
Genomic sequence	CATCCACTCCAAGCTTCAGTCCACATCGTAGGCTTGTAAGGCTTGTTAGGAGAAAATGTGTCAC AATAGAAACCATTGCATGCATTGATCTGTTTAAAGAACAGAGGTTAAATGTAATTTCAATAATT TGTTCGTACAGAAGTCATGAGATATTTGTATATTCCACGACTGAAATTTCACAAGATCAAAATC AATGTGGTTGGCGTCAATTTTTCTTCTTCTAATATATGTTTGATATATGAGTAGGCGACAAATA TGCTCATACATATGCTAGAAACCTAGAACACCAATCATGACCTCTTATGGAGATAACCCACTGA ACTCGCAAAAATACGAATCAGCATGTCCTGTATCGTTGTATAATTGTATTAACATGTTCGGTAT AGTTTCACAATTTACACCTGATGGTGGTAGTATTTGACAGAGGATAAGATCATCATACCACTGG GTCAGGTGCGTCATCCTCCTTGCACATCACCCACGGCACACCGGTGTCCAATCCCACGGCCATC TTTGCCGCCCAGTTGATATATGCCTTGCCGGCAGCCCCAAACTCTTTACCTTCTGGCCCATACT CGTTCTCAATCTGTGTCCAAACAATAACACCCACCGCACCATGAGCAACCCAGGAAAATGTGCA GGCTAAGATGAATCGTGCAAACAACACATTACTCAACCACTACTACGTACTAACCCAATGTTAT ACAATGTGCTTGCATCCATATACATGACATGGTTGAGGCCCTCTCTCTAAAAAAAATATACATG ACATGGGAAGCAAAGTTTTGCACTTGCCTGAGAGAGGATAATAGGACCGCCTTGTGAAGCAAAG AGGTTTTCACTCTTCATCATGCCCACAATTTTCTCTGTGAACCCCTGCATTGCATTCTGCAAAG CAAAACTCAAGTCAGAAACACATATGAACAAGATTTACACCTCTGGTAAGATCCAGAAATGCTG AAATACTTCCACAAACCTTGAAAGGTTCATTGTCCGTCCTGAAGCTGATGCCTGGTACATACTT CAACCAAACTGGAAATCCA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_013887 						
					
						PMCS
						0.395726575

Conservation Information
Conserved circRNAs	osi_circ_013887
PMCS	0.395726575

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3529833-3529017(+)
3529865-3528984(-)
3529068-3530007(-)
						
						Potential amino acid sequence
						
							MPTIFSVNPCIAFLKGSLSVLKLMPGTYFNQTGNPHPLQASVHIVGL*(+)
MQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVGLDTGVP
WVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFRTDNEPF
KNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVGLDT
GVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFRTDN
EPFKNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVG
LDTGVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFR
TDNEPFKNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKM
AVGLDTGVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSG(-)
MHAMVSIVTHFLLTSLTSLRCGLKLGVDVDFQFG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3529833-3529017(+) 3529865-3528984(-) 3529068-3530007(-)
Potential amino acid sequence	MPTIFSVNPCIAFLKGSLSVLKLMPGTYFNQTGNPHPLQASVHIVGL(+) MQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVGLDTGVP WVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFRTDNEPF KNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVGLDT GVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFRTDN EPFKNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKMAVG LDTGVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSGCGFPVWLKYVPGISFR TDNEPFKNAMQGFTEKIVGMMKSENLFASQGGPIILSQIENEYGPEGKEFGAAGKAYINWAAKM AVGLDTGVPWVMCKEDDAPDPVINACNGFYCDTFSPNKPYKPTMWTEAWSG(-) MHAMVSIVTHFLLTSLTSLRCGLKLGVDVDFQFG(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0165400_circ_g.4

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