Plant Circular RNA Database

Detail information of Os05g0589500_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os05g0589500_circ_g.1
					
						ID in PlantcircBase
						osa_circ_029152
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 29347844-29348049  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0589400						
					
						Parent gene annotation
						
							Similar to I-box binding factor (Fragment). (Os05t0589400-01);Si
milar to I-box binding factor (Fragment). (Os05t0589400-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						4
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0589500-01:1
Os05t0589400-02:1
Os05t0589400-01:1
Os05t0589500-01:1

General Information
CircRNA Name	Os05g0589500_circ_g.1
ID in PlantcircBase	osa_circ_029152
Alias	NA
Organism	Oryza sativa
Position	chr5: 29347844-29348049 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0589400
Parent gene annotation	Similar to I-box binding factor (Fragment). (Os05t0589400-01);Si milar to I-box binding factor (Fragment). (Os05t0589400-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	4
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0589500-01:1 Os05t0589400-02:1 Os05t0589400-01:1 Os05t0589500-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTGGTGACGAAGCTCCGGGAGATGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCA
GGAACAGCCTGTGCTCTTGCTCGCTCCATGGAACTCcatgtcgaagaggctggagcggcgcttg
ttgttggtcttcttggcggcgctgttgtggcggaggaagaacttctgggcatggctggcgacct
gggtgggc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 206
Transcript exons: 29347844-29348049
CATGTCGAAGAGGCTGGAGCGGCGCTTGTTGTTGGTCTTCTTGGCGGCGCTGTTGTGGCGGAGG
AAGAACTTCTGGGCATGGCTGGCGACCTGGGTGGGCGTCCTGGTGGTGACGAAGCTCCGGGAGA
TGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCAGGAACAGCCTGTGCTCTTGCTC
GCTCCATGGAACTC
						
						Genomic sequence
						
							CATGTCGAAGAGGCTGGAGCGGCGCTTGTTGTTGGTCTTCTTGGCGGCGCTGTTGTGGCGGAGG
AAGAACTTCTGGGCATGGCTGGCGACCTGGGTGGGCGTCCTGGTGGTGACGAAGCTCCGGGAGA
TGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCAGGAACAGCCTGTGCTCTTGCTC
GCTCCATGGAACTC

Sequences
Splice junction sequence	GTGGTGACGAAGCTCCGGGAGATGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCA GGAACAGCCTGTGCTCTTGCTCGCTCCATGGAACTCcatgtcgaagaggctggagcggcgcttg ttgttggtcttcttggcggcgctgttgtggcggaggaagaacttctgggcatggctggcgacct gggtgggc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 206 Transcript exons: 29347844-29348049 CATGTCGAAGAGGCTGGAGCGGCGCTTGTTGTTGGTCTTCTTGGCGGCGCTGTTGTGGCGGAGG AAGAACTTCTGGGCATGGCTGGCGACCTGGGTGGGCGTCCTGGTGGTGACGAAGCTCCGGGAGA TGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCAGGAACAGCCTGTGCTCTTGCTC GCTCCATGGAACTC
Genomic sequence	CATGTCGAAGAGGCTGGAGCGGCGCTTGTTGTTGGTCTTCTTGGCGGCGCTGTTGTGGCGGAGG AAGAACTTCTGGGCATGGCTGGCGACCTGGGTGGGCGTCCTGGTGGTGACGAAGCTCCGGGAGA TGCCTCGCCAGTCGCCCTTGCCCAGCTTCTCCAGCCCCGCCAGGAACAGCCTGTGCTCTTGCTC GCTCCATGGAACTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.470164482

Conservation Information
Conserved circRNAs	NA
PMCS	0.470164482

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							29347923-29347953(+)
29347846-29347953(+)
29348042-29347953(+)
29347925-29347846(-)
29348044-29347846(-)
29347848-29347846(-)
						
					

						Potential amino acid sequence
						
							MAGDLGGRPGGDEAPGDASPVALAQLLQPRQEQPVLLLAPWNSMSKRLERRLLLVFLAALLWRR
KNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPARNSLCSCSLHGTPCRRGWSGACCWSSWRR
CCGGGRTSGHGWRPGWASWW*(+)
MSKRLERRLLLVFLAALLWRRKNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPARNSLCSCS
LHGTPCRRGWSGACCWSSWRRCCGGGRTSGHGWRPGWASWW*(+)
MELHVEEAGAALVVGLLGGAVVAEEELLGMAGDLGGRPGGDEAPGDASPVALAQLLQPRQEQPV
LLLAPWNSMSKRLERRLLLVFLAALLWRRKNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPA
RNSLCSCSLHGTPCRRGWSGACCWSSWRRCCGGGRTSGHGWRPGWASWW*(+)
MPRSSSSATTAPPRRPTTSAAPASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAH
PGRQPCPEVLPPPQQRRQEDQQQAPLQPLRHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVT
TRTPTQVASHAQKFFLRHNSAAKKTNNKRRSSLFDMEFHGASKSTGCSWRGWRSWARATGEASP
GASSPPGRPPRSPAMPRSSSSATTAPPRRPTTSAAPASST(-)
MERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQRRQEDQQQAPLQPL
RHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVTTRTPTQVASHAQKFFLRHNSAAKKTNNKR
RSSLFDMEFHGASKSTGCSWRGWRSWARATGEASPGASSPPGRPPRSPAMPRSSSSATTAPPRR
PTTSAAPASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQ
RRQEDQQQAPLQPLRH(-)
MEFHGASKSTGCSWRGWRSWARATGEASPGASSPPGRPPRSPAMPRSSSSATTAPPRRPTTSAA
PASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQRRQEDQ
QQAPLQPLRHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVTTRTPTQVASHAQKFFLRHNSA
AKKTNNKRRSSLFDM(-)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	29347923-29347953(+) 29347846-29347953(+) 29348042-29347953(+) 29347925-29347846(-) 29348044-29347846(-) 29347848-29347846(-)
Potential amino acid sequence	MAGDLGGRPGGDEAPGDASPVALAQLLQPRQEQPVLLLAPWNSMSKRLERRLLLVFLAALLWRR KNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPARNSLCSCSLHGTPCRRGWSGACCWSSWRR CCGGGRTSGHGWRPGWASWW(+) MSKRLERRLLLVFLAALLWRRKNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPARNSLCSCS LHGTPCRRGWSGACCWSSWRRCCGGGRTSGHGWRPGWASWW(+) MELHVEEAGAALVVGLLGGAVVAEEELLGMAGDLGGRPGGDEAPGDASPVALAQLLQPRQEQPV LLLAPWNSMSKRLERRLLLVFLAALLWRRKNFWAWLATWVGVLVVTKLREMPRQSPLPSFSSPA RNSLCSCSLHGTPCRRGWSGACCWSSWRRCCGGGRTSGHGWRPGWASWW*(+) MPRSSSSATTAPPRRPTTSAAPASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAH PGRQPCPEVLPPPQQRRQEDQQQAPLQPLRHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVT TRTPTQVASHAQKFFLRHNSAAKKTNNKRRSSLFDMEFHGASKSTGCSWRGWRSWARATGEASP GASSPPGRPPRSPAMPRSSSSATTAPPRRPTTSAAPASST(-) MERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQRRQEDQQQAPLQPL RHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVTTRTPTQVASHAQKFFLRHNSAAKKTNNKR RSSLFDMEFHGASKSTGCSWRGWRSWARATGEASPGASSPPGRPPRSPAMPRSSSSATTAPPRR PTTSAAPASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQ RRQEDQQQAPLQPLRH(-) MEFHGASKSTGCSWRGWRSWARATGEASPGASSPPGRPPRSPAMPRSSSSATTAPPRRPTTSAA PASSTWSSMERARAQAVPGGAGEAGQGRLARHLPELRHHQDAHPGRQPCPEVLPPPQQRRQEDQ QQAPLQPLRHGVPWSEQEHRLFLAGLEKLGKGDWRGISRSFVTTRTPTQVASHAQKFFLRHNSA AKKTNNKRRSSLFDM(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0589500_circ_g.1

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