Plant Circular RNA Database

Detail information of Os02g0745600_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0745600_circ_g.2
					
						ID in PlantcircBase
						osa_circ_016562
					
						Alias
						Os_ciR2273
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 31295645-31296313  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
					
						Parent gene
						
							Os02g0745600						
					
						Parent gene annotation
						
							Kelch related domain containing protein. (Os02t0745600-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0745600_circ_g.1 						
					
						Support reads
						5/3
					
						Tissues
						root/root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0745600-01:2

General Information
CircRNA Name	Os02g0745600_circ_g.2
ID in PlantcircBase	osa_circ_016562
Alias	Os_ciR2273
Organism	Oryza sativa
Position	chr2: 31295645-31296313 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, find_circ
Parent gene	Os02g0745600
Parent gene annotation	Kelch related domain containing protein. (Os02t0745600-01)
Parent gene strand	+
Alternative splicing	Os02g0745600_circ_g.1
Support reads	5/3
Tissues	root/root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0745600-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TACCAGAGCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATACTTTTGACGAACG
GTACCATCAACCTCAAGTTGATATCGTGTATGAACGgtcaaatttaaggttgacaaagattgcc
ttcctcttccggagagtagtttcaagcatgccattaaggagaactacaattcaaagggaaagtt
cacccagg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 560
Transcript exons: 31295645-31295761,31295871-31296313
GTCAAATTTAAGGTTGACAAAGATTGCCTTCCTCTTCCGGAGAGTAGTTTCAAGCATGCCATTA
AGGAGAACTACAATTCAAAGGGAAAGTTCACCCAGGAACTCAGCCTTAAACAAGTTCATAGGTT
GTTAGAACTATTCAAGCCTATTAGCTTGCCTCAATCATCTATTCAATATGTTAAAGAAAGGCAT
CGACGATTGGATGTCTTTGAGGGTCGCCTACCACACTATGTTGAAGGAAGGAGACTGCCACGAC
ATGTCGAGGAAATGCATCATTTACGACATGTTGAAGAAAGGAGATTGCCATATGACCATGAAGA
AAGAAGATTACCGTATGACCATGAAGAAAGAAGACGACCACGATATGTTGAAGATATTCGTCAT
CCACAATTTCTTAAAGAAAGACATGCTATAACCGACTCTCTGCATGATCCATTTCGATCTCGGC
ATGTGACTCATTTACCAGAGCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATAC
TTTTGACGAACGGTACCATCAACCTCAAGTTGATATCGTGTATGAACG
						
						Genomic sequence
						
							GTCAAATTTAAGGTTGACAAAGATTGCCTTCCTCTTCCGGAGAGTAGTTTCAAGCATGCCATTA
AGGAGAACTACAATTCAAAGGGAAAGTTCACCCAGGAACTCAGCCTTAAACAAGTGAGTTGTGG
AGATTCTTCTATGTAGTTTTAAAAGAGTGCCAAAAGAAAATTAAATTGTAAACATCAAGTTGTG
TGCTGATGTCATAAGCTATGGTGCTATCTTGTAGGTTCATAGGTTGTTAGAACTATTCAAGCCT
ATTAGCTTGCCTCAATCATCTATTCAATATGTTAAAGAAAGGCATCGACGATTGGATGTCTTTG
AGGGTCGCCTACCACACTATGTTGAAGGAAGGAGACTGCCACGACATGTCGAGGAAATGCATCA
TTTACGACATGTTGAAGAAAGGAGATTGCCATATGACCATGAAGAAAGAAGATTACCGTATGAC
CATGAAGAAAGAAGACGACCACGATATGTTGAAGATATTCGTCATCCACAATTTCTTAAAGAAA
GACATGCTATAACCGACTCTCTGCATGATCCATTTCGATCTCGGCATGTGACTCATTTACCAGA
GCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATACTTTTGACGAACGGTACCAT
CAACCTCAAGTTGATATCGTGTATGAACG

Sequences
Splice junction sequence	TACCAGAGCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATACTTTTGACGAACG GTACCATCAACCTCAAGTTGATATCGTGTATGAACGgtcaaatttaaggttgacaaagattgcc ttcctcttccggagagtagtttcaagcatgccattaaggagaactacaattcaaagggaaagtt cacccagg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 560 Transcript exons: 31295645-31295761,31295871-31296313 GTCAAATTTAAGGTTGACAAAGATTGCCTTCCTCTTCCGGAGAGTAGTTTCAAGCATGCCATTA AGGAGAACTACAATTCAAAGGGAAAGTTCACCCAGGAACTCAGCCTTAAACAAGTTCATAGGTT GTTAGAACTATTCAAGCCTATTAGCTTGCCTCAATCATCTATTCAATATGTTAAAGAAAGGCAT CGACGATTGGATGTCTTTGAGGGTCGCCTACCACACTATGTTGAAGGAAGGAGACTGCCACGAC ATGTCGAGGAAATGCATCATTTACGACATGTTGAAGAAAGGAGATTGCCATATGACCATGAAGA AAGAAGATTACCGTATGACCATGAAGAAAGAAGACGACCACGATATGTTGAAGATATTCGTCAT CCACAATTTCTTAAAGAAAGACATGCTATAACCGACTCTCTGCATGATCCATTTCGATCTCGGC ATGTGACTCATTTACCAGAGCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATAC TTTTGACGAACGGTACCATCAACCTCAAGTTGATATCGTGTATGAACG
Genomic sequence	GTCAAATTTAAGGTTGACAAAGATTGCCTTCCTCTTCCGGAGAGTAGTTTCAAGCATGCCATTA AGGAGAACTACAATTCAAAGGGAAAGTTCACCCAGGAACTCAGCCTTAAACAAGTGAGTTGTGG AGATTCTTCTATGTAGTTTTAAAAGAGTGCCAAAAGAAAATTAAATTGTAAACATCAAGTTGTG TGCTGATGTCATAAGCTATGGTGCTATCTTGTAGGTTCATAGGTTGTTAGAACTATTCAAGCCT ATTAGCTTGCCTCAATCATCTATTCAATATGTTAAAGAAAGGCATCGACGATTGGATGTCTTTG AGGGTCGCCTACCACACTATGTTGAAGGAAGGAGACTGCCACGACATGTCGAGGAAATGCATCA TTTACGACATGTTGAAGAAAGGAGATTGCCATATGACCATGAAGAAAGAAGATTACCGTATGAC CATGAAGAAAGAAGACGACCACGATATGTTGAAGATATTCGTCATCCACAATTTCTTAAAGAAA GACATGCTATAACCGACTCTCTGCATGATCCATTTCGATCTCGGCATGTGACTCATTTACCAGA GCTTCAACATGCTCCTCCCACATACTATCATCATGTTGCTCATACTTTTGACGAACGGTACCAT CAACCTCAAGTTGATATCGTGTATGAACG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004295* osi_circ_012295 						
					
						PMCS
						0.245920179

Conservation Information
Conserved circRNAs	osi_circ_004295* osi_circ_012295
PMCS	0.245920179

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							31296022-31295883(+)
31296230-31295652(+)
31295708-31296165(-)
31295703-31296292(-)
						
						Potential amino acid sequence
						
							MHHLRHVEERRLPYDHEERRLPYDHEERRRPRYVEDIRHPQFLKERHAITDSLHDPFRSRHVTH
LPELQHAPPTYYHHVAHTFDERYHQPQVDIVYERSNLRLTKIAFLFRRVVSSMPLRRTTIQRES
SPRNSALNKFIGC*(+)
MLLPHTIIMLLILLTNGTINLKLISCMNGQI*(+)
MACLKLLSGRGRQSLSTLNLTVHTRYQLEVDGTVRQKYEQHDDSMWEEHVEALVNESHAEIEMD
HAESRL*(-)
MLETTLRKRKAIFVNLKFDRSYTIST*(-)
						
						Sponge-miRNAs
						
							osa-miR2928 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	31296022-31295883(+) 31296230-31295652(+) 31295708-31296165(-) 31295703-31296292(-)
Potential amino acid sequence	MHHLRHVEERRLPYDHEERRLPYDHEERRRPRYVEDIRHPQFLKERHAITDSLHDPFRSRHVTH LPELQHAPPTYYHHVAHTFDERYHQPQVDIVYERSNLRLTKIAFLFRRVVSSMPLRRTTIQRES SPRNSALNKFIGC(+) MLLPHTIIMLLILLTNGTINLKLISCMNGQI(+) MACLKLLSGRGRQSLSTLNLTVHTRYQLEVDGTVRQKYEQHDDSMWEEHVEALVNESHAEIEMD HAESRL(-) MLETTLRKRKAIFVNLKFDRSYTIST(-)
Sponge-miRNAs	osa-miR2928
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0745600_circ_g.2

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