Plant Circular RNA Database

Detail information of TGACv1_scaffold_641408_U_circ_ag.1

			
						 General Information
					
						CircRNA Name
						TGACv1_scaffold_641408_U_circ_ag.1
					
						ID in PlantcircBase
						tae_circ_000159
					
						Alias
						circRNA3645
					
						Organism
						
							Triticum aestivum						
					
						Position
						
							chrTGACv1_scaffold_641408_U: 124270-137099  JBrowse»						
					
						Reference genome
						
							TGACv1.38						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							NA						
					
						Support reads
						
						Tissues
						root
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-CA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	TGACv1_scaffold_641408_U_circ_ag.1
ID in PlantcircBase	tae_circ_000159
Alias	circRNA3645
Organism	Triticum aestivum
Position	chrTGACv1_scaffold_641408_U: 124270-137099 JBrowse»
Reference genome	TGACv1.38
Type	ag-circRNA
Identification method	find_circ
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	NA
Support reads
Tissues	root
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-CA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F:CGAGAAGCAAAGTGAGTTGATAA
R:ATGGTCATGGTGGCTATGTTAG
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F:CGAGAAGCAAAGTGAGTTGATAA R:ATGGTCATGGTGGCTATGTTAG PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGCTCCGCTGCACTCTTGATGGTCATGGTGGCTATGTTAGCGCCGTTGCAGTCAGCCCAGACGG
TTCGCTTTGCGCGTCTGGTGGCAAGGATGGCGTCACccttgctgtgggatttgaccgagggcaa
gagactctactcgctggatgcgggatccattatcaactcactttgcttctcgcccaaccgctac
tggctctg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	AGCTCCGCTGCACTCTTGATGGTCATGGTGGCTATGTTAGCGCCGTTGCAGTCAGCCCAGACGG TTCGCTTTGCGCGTCTGGTGGCAAGGATGGCGTCACccttgctgtgggatttgaccgagggcaa gagactctactcgctggatgcgggatccattatcaactcactttgcttctcgcccaaccgctac tggctctg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.243826578

Conservation Information
Conserved circRNAs	NA
PMCS	0.243826578

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						involved in the regulation of wheat root length (acting as miRNA sponges) (validated)

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	involved in the regulation of wheat root length (acting as miRNA sponges) (validated)

			
						 Other Information
					
						References
						Xu et al., 2019

circRNA

Detail information of TGACv1_scaffold_641408_U_circ_ag.1

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