Plant Circular RNA Database

Detail information of Zm00001d018460_circ_g.4

			
						 General Information
					
						CircRNA Name
						Zm00001d018460_circ_g.4
					
						ID in PlantcircBase
						zma_circ_008842
					
						Alias
						Zm05circ00141, zma_circ_0002082, GRMZM2G079013_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr5: 221202380-221202672  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d018460						
					
						Parent gene annotation
						
							 leunig-related2
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d018460_circ_g.2 Zm00001d018460_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d018460_T002:1
Zm00001d018460_T008:1
Zm00001d018460_T004:1
Zm00001d018460_T005:1
Zm00001d018460_T009:1
Zm00001d018460_T012:1
Zm00001d018460_T011:1
Zm00001d018460_T010:1
Zm00001d018460_T003:1
Zm00001d018460_T006:1
Zm00001d018460_T007:1

General Information
CircRNA Name	Zm00001d018460_circ_g.4
ID in PlantcircBase	zma_circ_008842
Alias	Zm05circ00141, zma_circ_0002082, GRMZM2G079013_C1
Organism	Zea mays
Position	chr5: 221202380-221202672 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d018460
Parent gene annotation	leunig-related2
Parent gene strand	-
Alternative splicing	Zm00001d018460_circ_g.2 Zm00001d018460_circ_g.3
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d018460_T002:1 Zm00001d018460_T008:1 Zm00001d018460_T004:1 Zm00001d018460_T005:1 Zm00001d018460_T009:1 Zm00001d018460_T012:1 Zm00001d018460_T011:1 Zm00001d018460_T010:1 Zm00001d018460_T003:1 Zm00001d018460_T006:1 Zm00001d018460_T007:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCAT
CTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGCcctgcatggtttgttgctgacttgagaa
gagccattctactagcttcaataagttgtgatccctcagactccattgaatgtgggtgcttcaa
tcgctctt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 293
Transcript exons: 221202380-221202672
CCTGCATGGTTTGTTGCTGACTTGAGAAGAGCCATTCTACTAGCTTCAATAAGTTGTGATCCCT
CAGACTCCATTGAATGTGGGTGCTTCAATCGCTCTTCATACATCTTGGCAGCTAAGACACTAGC
TGTTGATGGCCCCAAAATGCCCTCAGAATTTAAAGCATTTATTGGACCATTAAGTGAAGGATGA
TTCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCA
TCTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGC
						
						Genomic sequence
						
							CCTGCATGGTTTGTTGCTGACTTGAGAAGAGCCATTCTACTAGCTTCAATAAGTTGTGATCCCT
CAGACTCCATTGAATGTGGGTGCTTCAATCGCTCTTCATACATCTTGGCAGCTAAGACACTAGC
TGTTGATGGCCCCAAAATGCCCTCAGAATTTAAAGCATTTATTGGACCATTAAGTGAAGGATGA
TTCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCA
TCTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGC

Sequences
Splice junction sequence	TCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCAT CTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGCcctgcatggtttgttgctgacttgagaa gagccattctactagcttcaataagttgtgatccctcagactccattgaatgtgggtgcttcaa tcgctctt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 293 Transcript exons: 221202380-221202672 CCTGCATGGTTTGTTGCTGACTTGAGAAGAGCCATTCTACTAGCTTCAATAAGTTGTGATCCCT CAGACTCCATTGAATGTGGGTGCTTCAATCGCTCTTCATACATCTTGGCAGCTAAGACACTAGC TGTTGATGGCCCCAAAATGCCCTCAGAATTTAAAGCATTTATTGGACCATTAAGTGAAGGATGA TTCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCA TCTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGC
Genomic sequence	CCTGCATGGTTTGTTGCTGACTTGAGAAGAGCCATTCTACTAGCTTCAATAAGTTGTGATCCCT CAGACTCCATTGAATGTGGGTGCTTCAATCGCTCTTCATACATCTTGGCAGCTAAGACACTAGC TGTTGATGGCCCCAAAATGCCCTCAGAATTTAAAGCATTTATTGGACCATTAAGTGAAGGATGA TTCGCGTTAGTTCGCTGAAGTTGAGCATGTCTTTGATGCATAAGTTGCAATTGCTGCATCTGCA TCTGCTGCTGGTGCTCTCTTGCTTTTATTTGCTGTGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.35609583

Conservation Information
Conserved circRNAs	NA
PMCS	0.35609583

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							221202569-221202568(+)
221202638-221202662(-)
221202388-221202580(-)
						
						Potential amino acid sequence
						
							MIRVSSLKLSMSLMHKLQLLHLHLLLVLSCFYLLCPAWFVADLRRAILLASISCDPSDSIECGC
FNRSSYILAAKTLAVDGPKMPSEFKAFIGPLSEG*(+)
MQMQQLQLMHQRHAQLQRTNANHPSLNGPINALNSEGILGPSTASVLAAKMYEERLKHPHSMES
EGSQLIEASRMALLKSATNHAGHSK*(-)
MQGTANKSKRAPAADADAAIATYASKTCSTSAN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	221202569-221202568(+) 221202638-221202662(-) 221202388-221202580(-)
Potential amino acid sequence	MIRVSSLKLSMSLMHKLQLLHLHLLLVLSCFYLLCPAWFVADLRRAILLASISCDPSDSIECGC FNRSSYILAAKTLAVDGPKMPSEFKAFIGPLSEG(+) MQMQQLQLMHQRHAQLQRTNANHPSLNGPINALNSEGILGPSTASVLAAKMYEERLKHPHSMES EGSQLIEASRMALLKSATNHAGHSK(-) MQGTANKSKRAPAADADAAIATYASKTCSTSAN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d018460_circ_g.4

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